FastQCFastQC Report
Wed 25 May 2016
SRR1294728_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294728_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194450
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC112170.3511402588865063No Hit
CCCATGTACTCTGCGTTGATACCAC101050.3163298846436789No Hit
GAGTACATGGGAAGCAGTGGTATCA52840.16541188624019784No Hit
GTATCAACGCAGAGTACTTTTTTTT44690.1398988871323702No Hit
CATGTACTCTGCGTTGATACCACTG37930.11873718480489598No Hit
ATTCCATTCCATTCCATTCCATTCC35380.11075458999201741No Hit
GCGTTGATACCACTGCTTCCCATGT34500.10799981217423969No Hit
GGTATCAACGCAGAGTACTTTTTTT33930.10621546745136096No Hit
GTATCAACGCAGAGTACATGGGAAG32530.10183286637762369No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC701.0916206E-412.2142563
AGTCCGC600.005897965711.0782756
CTGGACG1409.7132215E-810.177594
TATCGCG750.002658929210.1287097
GCCGTTC750.002658929210.1287098
GTATTAG4750.010.0021681
GTCTTAA3250.09.6482461
CTACGTC900.00111517369.4990844
TCGCGCG900.0011186929.4958139
GTCTAGG3250.09.3558741
GTCCTGG6050.09.2664721
TACACTG7800.09.1323045
GTATAGA4700.09.0977171
TCTAGAC2304.1836756E-119.0869353
TGCTCGG1151.11661226E-49.08295210
GTTCTAG3700.08.9884351
CTATCGG2451.6370905E-118.91429612
ACTATCG2252.6557245E-108.86261911
TTACACT5150.08.8535154
TAGACTG4200.08.8191975
Produced by FastQC (version 0.11.5)