##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294728_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3194450
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.99309552505126	33.0	33.0	33.0	27.0	33.0
2	30.981018015620844	33.0	33.0	33.0	27.0	33.0
3	30.965678285776896	33.0	33.0	33.0	27.0	33.0
4	34.353768880401944	37.0	37.0	37.0	27.0	37.0
5	34.72262392587143	37.0	37.0	37.0	27.0	37.0
6	34.93972702656169	37.0	37.0	37.0	33.0	37.0
7	34.84514047801656	37.0	37.0	37.0	33.0	37.0
8	34.79781809075115	37.0	37.0	37.0	27.0	37.0
9	34.472445647920615	37.0	37.0	37.0	27.0	37.0
10	34.55688177933604	37.0	37.0	37.0	27.0	37.0
11	34.809458592245925	37.0	37.0	37.0	33.0	37.0
12	34.73311180328382	37.0	37.0	37.0	27.0	37.0
13	34.66652412778413	37.0	37.0	37.0	27.0	37.0
14	35.78579661600588	40.0	37.0	40.0	27.0	40.0
15	36.11026812127283	40.0	37.0	40.0	27.0	40.0
16	36.12614440670538	40.0	37.0	40.0	27.0	40.0
17	36.06209206592684	40.0	37.0	40.0	27.0	40.0
18	36.05736198719654	40.0	37.0	40.0	27.0	40.0
19	36.14049429479253	40.0	37.0	40.0	27.0	40.0
20	36.03416300145565	40.0	37.0	40.0	27.0	40.0
21	35.99161671023181	40.0	37.0	40.0	27.0	40.0
22	35.852354239383935	37.0	37.0	40.0	27.0	40.0
23	35.46111224154393	37.0	33.0	40.0	27.0	40.0
24	35.78662555369469	37.0	37.0	40.0	27.0	40.0
25	34.63421496658267	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6595017862145909
1101	2	0.7998955383923274
1101	3	0.631831587325479
1101	4	0.7228666113688007
1101	5	0.767042010337974
1101	6	0.5907583796887224
1101	7	0.6926241912420465
1101	8	0.7273780973146913
1101	9	0.9856294258454099
1101	10	0.9937044631865533
1101	11	0.8013252577408707
1101	12	0.7540642457191709
1101	13	0.7265124986754401
1101	14	1.4346185278271761
1101	15	1.232275297518747
1101	16	1.0969274709265733
1101	17	1.1217475245175166
1101	18	1.1504195045012153
1101	19	1.018110826464003
1101	20	1.2259838415129352
1101	21	1.210682762703449
1101	22	1.300160689681988
1101	23	1.605248250061969
1101	24	1.3329455066895832
1101	25	1.2297200490022817
1102	1	0.3761633084344034
1102	2	0.5448755726284276
1102	3	0.4133243955158541
1102	4	0.32310746316030503
1102	5	0.3736733679904063
1102	6	0.41039879306534033
1102	7	0.40434377672534794
1102	8	0.49038888449585016
1102	9	0.7536763700689377
1102	10	0.7992819059013101
1102	11	0.5627522269963237
1102	12	0.638328215615509
1102	13	0.6963309571141352
1102	14	1.3233353602712015
1102	15	1.0349738015716952
1102	16	0.7358673617753411
1102	17	0.9191711891636629
1102	18	0.9366648103352873
1102	19	0.80564848451845
1102	20	0.9705537269513016
1102	21	0.8790150773591492
1102	22	0.9543402611493335
1102	23	1.3603577841175962
1102	24	1.0871722473110168
1102	25	0.9849213979320695
1103	1	0.48307434652732795
1103	2	0.5957927645421712
1103	3	0.4700464756992453
1103	4	0.23869022323624023
1103	5	0.529675479293509
1103	6	0.4350335992065695
1103	7	0.4052210662853142
1103	8	0.6133321380674133
1103	9	0.7833857055374835
1103	10	0.7654891033943443
1103	11	0.5622236486370369
1103	12	0.5734112230032551
1103	13	0.5684217674075001
1103	14	1.1828227641028093
1103	15	0.9708716341923562
1103	16	0.6770469494603191
1103	17	0.7090480547541986
1103	18	0.7473474320998861
1103	19	0.8156879760508602
1103	20	1.0321571884400953
1103	21	0.9987727830599127
1103	22	1.0080833551557546
1103	23	1.3791071314185004
1103	24	1.1021693965737072
1103	25	1.075396747919335
1104	1	0.38825639678669077
1104	2	0.5831289251906959
1104	3	0.4714448229230648
1104	4	0.46284581950988724
1104	5	0.42492503444938023
1104	6	0.41298350765141123
1104	7	0.32018095095064325
1104	8	0.45378366945484316
1104	9	0.7429575895028577
1104	10	0.7598846858419464
1104	11	0.5314800775440247
1104	12	0.4719377598120289
1104	13	0.46166562070438744
1104	14	0.983474164167049
1104	15	0.8032938706984964
1104	16	0.6913581241887599
1104	17	0.7039698555202563
1104	18	0.7215898087677104
1104	19	0.7913789127696376
1104	20	0.8811279465828576
1104	21	0.9770884536744902
1104	22	1.0101711015738033
1104	23	1.3638153144485372
1104	24	1.1363262721715799
1104	25	0.9726423170157759
1105	1	0.333519711020859
1105	2	0.3701694509465092
1105	3	0.2973241426080726
1105	4	0.2898577084931162
1105	5	0.28934653091923934
1105	6	0.26928991153599924
1105	7	0.2526704782351388
1105	8	0.4271220060101584
1105	9	-1.7443020753223308
1105	10	-0.08214953113157009
1105	11	0.17787325755701033
1105	12	0.3074475973715778
1105	13	0.3600257821900499
1105	14	-1.4885494577481637
1105	15	-0.22675335191442514
1105	16	0.12264090650035087
1105	17	0.3144984551622301
1105	18	0.23021600340673842
1105	19	0.40976294735826
1105	20	0.5946824517583877
1105	21	0.6553421820501839
1105	22	0.5873790812679758
1105	23	1.095612244550928
1105	24	0.818239761604076
1105	25	0.42633445186184815
1106	1	0.05698432620863869
1106	2	0.14792011550694184
1106	3	0.19160976550643838
1106	4	-1.1253273899450562
1106	5	-0.27336298747091803
1106	6	-0.03891204880120824
1106	7	0.047324313528712025
1106	8	0.19760988227483267
1106	9	0.45219225446128775
1106	10	0.5169658442638223
1106	11	0.2655766625758531
1106	12	0.2744025468980027
1106	13	0.25764684671801774
1106	14	0.6719463151694143
1106	15	0.4336095777025264
1106	16	0.2619364363275807
1106	17	0.24905786767326532
1106	18	0.2886435823982012
1106	19	0.2611966748019796
1106	20	0.5055482083151688
1106	21	0.609770911146498
1106	22	0.7916582864162081
1106	23	0.8967100547086488
1106	24	0.5755918859447178
1106	25	0.6297778190001466
1107	1	0.4535948634950202
1107	2	0.39020343363506527
1107	3	0.37728875068479795
1107	4	0.6133564960479632
1107	5	0.66464239471469
1107	6	0.2760989310408135
1107	7	0.345882798570301
1107	8	0.4581362423005686
1107	9	0.5492490149723679
1107	10	0.562410470559513
1107	11	0.2977323944812724
1107	12	0.456824866616941
1107	13	0.48080766587383295
1107	14	0.8546296449115687
1107	15	0.7358266347381033
1107	16	0.8034440595623664
1107	17	0.6492267295442531
1107	18	0.6817804552597266
1107	19	0.7098108613385676
1107	20	0.8806226679953824
1107	21	0.7774305773042869
1107	22	0.6336553798525983
1107	23	1.0684572478575944
1107	24	0.961975964944564
1107	25	0.9421518029636218
1108	1	0.4672216407581615
1108	2	0.46000837616728063
1108	3	0.5977379984067746
1108	4	0.6638938374943919
1108	5	0.6033037333760234
1108	6	0.42326691457243015
1108	7	0.4248291047893318
1108	8	0.5356546834553697
1108	9	0.3333194899519327
1108	10	0.559979568128611
1108	11	0.3994750962239735
1108	12	0.4451416003882187
1108	13	0.4163539377837395
1108	14	-0.9362209186662724
1108	15	0.06341419868930842
1108	16	0.45230328114885765
1108	17	0.5993513523961482
1108	18	0.6586197775535965
1108	19	0.6399877551518287
1108	20	0.7587001460728615
1108	21	0.7848655665574569
1108	22	0.9324565786537988
1108	23	1.0539626515448148
1108	24	1.0477326325194127
1108	25	0.9044834107497763
1109	1	0.39004117399793614
1109	2	0.3625902318473351
1109	3	0.4885840198810101
1109	4	0.5268988106515948
1109	5	0.42898728724354385
1109	6	0.27424669522631007
1109	7	0.23229584830882288
1109	8	0.3896313064607213
1109	9	0.4664150250629078
1109	10	0.4853603979905756
1109	11	0.323753255171944
1109	12	0.26764685385402487
1109	13	0.2513654100164828
1109	14	0.715682441695499
1109	15	0.4450977488441268
1109	16	0.47561228703719394
1109	17	0.39830766695066444
1109	18	0.4732353185306337
1109	19	0.44311899712312197
1109	20	0.6168206026603045
1109	21	0.6606779509997764
1109	22	0.6565336022591239
1109	23	0.9876664191894093
1109	24	0.7988768392275105
1109	25	0.5685129025558524
1110	1	0.30229653062130524
1110	2	0.18214246250074595
1110	3	0.3554661245656767
1110	4	0.6183287381790947
1110	5	0.4590750828574599
1110	6	0.25094692028787335
1110	7	0.24313815702171127
1110	8	0.30130883213465864
1110	9	0.3394854477867
1110	10	0.41843067769255526
1110	11	0.1474854885153789
1110	12	0.20486910244905943
1110	13	0.14839579028409133
1110	14	0.5577847999730494
1110	15	0.35176662017683213
1110	16	0.3204627181718891
1110	17	0.3383884118934688
1110	18	0.32380038402244793
1110	19	0.35178697867756625
1110	20	0.354756370346621
1110	21	0.39045036829968893
1110	22	0.4260967040918757
1110	23	0.6479309578132089
1110	24	0.6363993803782364
1110	25	0.6394513036693539
1111	1	0.05817037512094814
1111	2	0.09258083645674375
1111	3	0.19529555876307825
1111	4	0.3827491534838856
1111	5	0.36640342423310557
1111	6	0.2859153846277849
1111	7	0.11876784497282245
1111	8	0.2391232461965913
1111	9	0.2077338177878616
1111	10	0.170084452620209
1111	11	0.18522910141007998
1111	12	0.175427458196161
1111	13	0.13505026185874414
1111	14	-1.7753166541481207
1111	15	-0.6342369247788042
1111	16	-0.04062566463097994
1111	17	-0.021068317841773876
1111	18	0.1424768339781295
1111	19	0.1717795624805376
1111	20	0.2672348153183677
1111	21	0.27954311932411
1111	22	0.010200128002523456
1111	23	-2.040008981682611
1111	24	-0.4639516198008664
1111	25	0.0010417508240152529
1112	1	-0.17854172138128988
1112	2	-0.3356997727475921
1112	3	-0.04227110346409546
1112	4	0.09256769580710511
1112	5	-0.6970654180205216
1112	6	-0.8477055749554765
1112	7	-1.0096585398477558
1112	8	-0.7720015803030122
1112	9	-0.6454643006313603
1112	10	-0.9920006054269024
1112	11	-0.8543127825639019
1112	12	-0.8350533845078871
1112	13	-0.9100134992624049
1112	14	-0.6548958372166567
1112	15	-0.9258987403434347
1112	16	-0.9650691623755137
1112	17	-0.9697882737173273
1112	18	-1.1326373947745623
1112	19	-0.8846277289904307
1112	20	-1.033463170063058
1112	21	-1.7419858510162527
1112	22	-1.7530432335583441
1112	23	-1.408202812021642
1112	24	-1.800473229696415
1112	25	-1.98024269751307
1113	1	0.04796250302395322
1113	2	-0.1854765246751633
1113	3	-0.18090756086138882
1113	4	0.12337022095002226
1113	5	-0.031033806668709474
1113	6	-0.222267714720374
1113	7	-0.036920777781666914
1113	8	0.02554429606097841
1113	9	0.18560818929680067
1113	10	-0.10986795407622907
1113	11	-0.29422657018240983
1113	12	-0.21292429792130463
1113	13	-0.2633710668905138
1113	14	-0.15112087696798682
1113	15	-0.24370780931246827
1113	16	-0.09724157958481072
1113	17	-0.08194637085961887
1113	18	0.016351150624466015
1113	19	-0.24112549207917056
1113	20	-0.806441081599381
1113	21	-0.843373102060184
1113	22	-0.26302246670118024
1113	23	-0.5054472595456687
1113	24	-0.2598638990789226
1113	25	-1.3641625609189845
1114	1	-0.986226218720077
1114	2	-0.777120624960812
1114	3	-0.5215210014915357
1114	4	-1.5489721146596978
1114	5	-0.7981858825774282
1114	6	-0.7559692501545499
1114	7	-0.5886246446653232
1114	8	-0.482750247214355
1114	9	-0.4992177302524823
1114	10	-0.5822535105134463
1114	11	-0.6367397280207783
1114	12	-0.5985702231921124
1114	13	-0.5961300265252092
1114	14	-0.6654925161083511
1114	15	-1.4385433012278384
1114	16	-0.8980354528897152
1114	17	-0.9054202305889945
1114	18	-0.7911191029266149
1114	19	-0.7858559545277473
1114	20	-1.9239422323301127
1114	21	-6.776088555267215
1114	22	-7.714560161879152
1114	23	-8.122592600050108
1114	24	-5.86211947411347
1114	25	-7.415413989514889
1115	1	-1.0981814373830758
1115	2	-1.1787217197212243
1115	3	-0.9307040549127983
1115	4	-0.6244557914649249
1115	5	-0.8288190340760835
1115	6	-0.9869968554082718
1115	7	-1.1669252848964646
1115	8	-1.0029474511184588
1115	9	-3.0930839016120473
1115	10	-1.93743599036479
1115	11	-1.3377958164960404
1115	12	-1.2927587500325401
1115	13	-1.6442170100490472
1115	14	-1.3141872999532822
1115	15	-2.2457815774090264
1115	16	-1.5405419610703603
1115	17	-3.1114424083703938
1115	18	-2.974946384101841
1115	19	-2.291345799698327
1115	20	-2.064134703708092
1115	21	-1.6280288335443487
1115	22	-1.0753771046289842
1115	23	-0.6917892920296609
1115	24	-1.6097245899895256
1115	25	-1.2939167193324437
1116	1	-1.8462706446248838
1116	2	-1.017515309807628
1116	3	-0.8292922641506131
1116	4	-0.4149705670558248
1116	5	-0.549473800321671
1116	6	-0.6150265875542615
1116	7	-0.926592959020482
1116	8	-1.9818397342112704
1116	9	-1.0815494603457267
1116	10	-2.5425164924188692
1116	11	-1.2997909061543425
1116	12	-1.0298080614232958
1116	13	-0.9397904099339911
1116	14	-1.5267721437973947
1116	15	-1.6433150625362032
1116	16	-1.1522055081304998
1116	17	-0.946812534154013
1116	18	-1.5056784247566597
1116	19	-1.0122066490415946
1116	20	-1.3368396321462086
1116	21	-0.8708422594665208
1116	22	-0.50918094485111
1116	23	-0.46249091989706415
1116	24	-0.3243346121643924
1116	25	-0.5221881872221061
1201	1	0.4306674418703942
1201	2	0.502955648383498
1201	3	0.44848633505742086
1201	4	0.552873139662104
1201	5	0.6092864737567467
1201	6	0.5496031532317289
1201	7	0.494933976198908
1201	8	0.562947613134213
1201	9	0.880689501361374
1201	10	0.7246477429880045
1201	11	0.5735694756135814
1201	12	0.6697380256952599
1201	13	0.6653245288525511
1201	14	1.3943937728244649
1201	15	1.1320243153232639
1201	16	1.012344617107594
1201	17	0.9379735317602709
1201	18	0.8462736679143177
1201	19	0.8688818433555525
1201	20	1.1068561576035307
1201	21	1.166875369396692
1201	22	1.232219888818456
1201	23	0.9672784518689639
1201	24	1.1014758290924647
1201	25	0.9668913556292082
1202	1	-0.15349521546545475
1202	2	0.0569049860509061
1202	3	0.014335258423500363
1202	4	0.01749854938000084
1202	5	0.14241459796553357
1202	6	0.11471421855153352
1202	7	0.07183905471801211
1202	8	0.20425430358799446
1202	9	0.22638572215326036
1202	10	0.4914582514482362
1202	11	0.2180713257185829
1202	12	0.2620882989790516
1202	13	0.4031649355065028
1202	14	0.6357359547365817
1202	15	0.6425241591086817
1202	16	0.49746223252015653
1202	17	0.2655439093371328
1202	18	0.29364628538770887
1202	19	0.3816193997155892
1202	20	0.5234292804632616
1202	21	0.4803949668666121
1202	22	0.5189219051780327
1202	23	0.9121122673594471
1202	24	0.5678130341100598
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2211	5	0.32393795195186215
2211	6	0.17949516055979586
2211	7	0.18937461530313016
2211	8	0.3339537464863369
2211	9	0.4315543219408511
2211	10	0.33020373019409277
2211	11	0.19783292601893265
2211	12	0.19238346553631658
2211	13	0.13694566460164737
2211	14	0.4882952349768601
2211	15	0.32924337269076887
2211	16	0.19264167166416968
2211	17	0.3080436195737164
2211	18	0.24002367577839578
2211	19	0.3348143799423866
2211	20	0.37804344000448253
2211	21	0.3315520047790059
2211	22	0.32227758598977374
2211	23	0.49188086765097694
2211	24	0.46986622837374625
2211	25	0.41734825072743575
2212	1	-0.39019326485686534
2212	2	-0.39127355321104673
2212	3	-0.3463692467987016
2212	4	-0.5620886404709964
2212	5	-0.41909668499754815
2212	6	-0.6568817348257596
2212	7	-0.6715196349395214
2212	8	-0.41124568077263035
2212	9	-0.2692282571961826
2212	10	-0.7771743294279716
2212	11	-0.5612733276116941
2212	12	-0.7279182053744506
2212	13	-0.7712208425305178
2212	14	-0.5427529873703918
2212	15	-0.8643186338635402
2212	16	-0.6752940554173605
2212	17	-0.7326534335439661
2212	18	-0.5432322650550034
2212	19	-0.7036411601671446
2212	20	-0.5770155923274416
2212	21	-0.648156363718897
2212	22	-0.5492105238186724
2212	23	-0.15344657538850726
2212	24	-0.34714176296510857
2212	25	-1.258218743715652
2213	1	-0.13369162381014732
2213	2	-1.3224131151854799
2213	3	-0.3214582461380431
2213	4	0.0056860172024428834
2213	5	-0.05778609528339018
2213	6	-0.04096595811449788
2213	7	-0.03802959225410518
2213	8	0.03727242670482411
2213	9	0.06874860747775102
2213	10	-0.38396132191726906
2213	11	-0.10836814092992597
2213	12	-0.073516979510444
2213	13	-0.4358504813839801
2213	14	-0.14415277531676907
2213	15	-0.11048829325064702
2213	16	0.061543462701934004
2213	17	0.19441693589642028
2213	18	0.08355313097224837
2213	19	0.1023735428607111
2213	20	0.18452601706966476
2213	21	-0.45533760102712506
2213	22	-0.9786476045408037
2213	23	-0.18846923682902172
2213	24	-0.0846950674914595
2213	25	-0.6881519738374493
2214	1	-0.24501161696379725
2214	2	-0.48851400814831436
2214	3	-0.5487170614002927
2214	4	-0.7308521664774403
2214	5	-0.4746980444190996
2214	6	-0.3443386062481295
2214	7	-0.6111725034032816
2214	8	-0.23364937621809645
2214	9	-0.163618016549556
2214	10	-2.1991043875550034
2214	11	-1.0679532735151227
2214	12	-0.6592944368779001
2214	13	-0.3643227688489432
2214	14	-0.862856409226751
2214	15	-0.6183309742006387
2214	16	-0.7882975863968298
2214	17	-0.559029743562867
2214	18	-0.541859486321222
2214	19	-1.465123586975551
2214	20	-2.555058735786716
2214	21	-1.9986164203470693
2214	22	-2.191845539167012
2214	23	-2.584241697007421
2214	24	-1.8949677426097011
2214	25	-2.0615672158220875
2215	1	-0.19819868251919104
2215	2	-0.3143258180045194
2215	3	-0.8239523510816973
2215	4	-0.31495255625408447
2215	5	-0.15701145250833548
2215	6	-0.16965803625731013
2215	7	-0.09381021905616649
2215	8	-0.24965100839386167
2215	9	-0.32569864876688825
2215	10	-0.20540128569357563
2215	11	-0.538079079508833
2215	12	-0.408406963238356
2215	13	-0.2519444623248859
2215	14	-0.12139297034047303
2215	15	-0.7125040577256883
2215	16	-0.40065174733554443
2215	17	-0.2718585288439854
2215	18	-0.2073547722292659
2215	19	-2.260060559320941
2215	20	-2.444333442249693
2215	21	-3.353144276913298
2215	22	-2.546919367447188
2215	23	-3.5114186847328703
2215	24	-2.721131664923135
2215	25	-1.783485159801188
2216	1	-1.5215203673579154
2216	2	-1.3202962670285672
2216	3	-0.918848991354583
2216	4	-1.0052631356864197
2216	5	-2.058962961677551
2216	6	-1.7272288886804503
2216	7	-1.1874217839146155
2216	8	-5.398300581349371
2216	9	-4.349534169056717
2216	10	-2.294076212156675
2216	11	-1.3167977020160393
2216	12	-1.319143791318929
2216	13	-2.045085020674591
2216	14	-3.4939689331952053
2216	15	-2.037333538422068
2216	16	-3.2936177129092457
2216	17	-2.984870684881031
2216	18	-2.888789593998844
2216	19	-1.7601515987878216
2216	20	-1.0270496232834603
2216	21	-0.9570393243787336
2216	22	-0.918050837427387
2216	23	-1.0602108054006223
2216	24	-0.6933206510077881
2216	25	-0.6291937913327956
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	37.0
8	252.0
9	658.0
10	1451.0
11	2872.0
12	5111.0
13	7929.0
14	10717.0
15	13393.0
16	15331.0
17	16401.0
18	17359.0
19	17918.0
20	18811.0
21	20190.0
22	21217.0
23	22856.0
24	24874.0
25	26886.0
26	29470.0
27	33247.0
28	38085.0
29	45116.0
30	54221.0
31	66994.0
32	88876.0
33	125809.0
34	181744.0
35	283659.0
36	486280.0
37	1516681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.043066270349755	20.69083648670823	12.85195985224544	23.414137390696574
2	15.647240603587145	20.546447060886283	39.949841964616986	23.85647037090959
3	19.092515374766688	25.266281471656605	28.788645921494986	26.852557232081715
4	12.524094371028891	16.464674740081385	37.40576736892599	33.60546351996373
5	13.56555517598872	37.80954310346717	34.76316960364768	13.861732116896427
6	32.42098935880458	37.85278896997361	16.511416039747164	13.214805631474647
7	27.209247142042326	32.49081052111592	22.33470677250976	17.965235564331987
8	24.586906216817262	37.43967628673893	20.342802726744537	17.630614769699278
9	24.24651380469167	17.23474158562603	21.681888689059083	36.836855920623215
10	14.998981034724022	30.456512095947396	32.86571540284189	21.678791466486686
11	33.21061904350472	25.820526594565763	21.937464547466014	19.031389814463505
12	20.93594239429803	27.5899807350725	31.00019221100887	20.4738846596206
13	26.775790007848187	23.764238736500847	26.28054321873473	23.17942803691623
14	20.410208281550428	23.027642524822927	27.716215683193163	28.845933510433486
15	23.416227797355546	30.51534521690104	23.297425512219416	22.771001473523995
16	22.08063992526859	31.157945549877862	25.35652536507164	21.404889159781902
17	20.551287120282222	30.237896004199875	28.04983378616	21.160983089357902
18	20.81672084508312	29.792911559787427	28.81009296315033	20.580274631979123
19	21.293380924445458	28.596176975326458	29.159970123032753	20.950471977195335
20	22.50578114390736	28.24209712071329	28.244382343430157	21.007739391949194
21	22.570915936618576	28.751002925205043	27.736658235985757	20.941422902190627
22	21.394927292190562	28.308975217283834	28.800794981740417	21.49530250878518
23	21.41451159800637	28.644433173108403	28.570403678479533	21.37065155040569
24	21.44048509740453	28.146825235332972	28.488609790227304	21.924079877035197
25	22.105393618022653	27.906717705751312	27.68234445106837	22.305544225157668
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2264.0
1	2264.0
2	2683.5
3	3103.0
4	3103.0
5	3103.0
6	4538.5
7	5974.0
8	5974.0
9	5974.0
10	8824.5
11	11675.0
12	11675.0
13	11675.0
14	19096.5
15	26518.0
16	26518.0
17	26518.0
18	41613.5
19	56709.0
20	56709.0
21	56709.0
22	81307.0
23	105905.0
24	105905.0
25	105905.0
26	138199.0
27	170493.0
28	170493.0
29	170493.0
30	206990.5
31	243488.0
32	243488.0
33	243488.0
34	276722.5
35	309957.0
36	309957.0
37	309957.0
38	336982.5
39	364008.0
40	364008.0
41	364008.0
42	367094.0
43	370180.0
44	370180.0
45	370180.0
46	383538.0
47	396896.0
48	396896.0
49	396896.0
50	374338.0
51	351780.0
52	351780.0
53	351780.0
54	317307.0
55	282834.0
56	282834.0
57	282834.0
58	247109.5
59	211385.0
60	211385.0
61	211385.0
62	176987.0
63	142589.0
64	142589.0
65	142589.0
66	110841.0
67	79093.0
68	79093.0
69	79093.0
70	58023.5
71	36954.0
72	36954.0
73	36954.0
74	26031.0
75	15108.0
76	15108.0
77	15108.0
78	10188.5
79	5269.0
80	5269.0
81	5269.0
82	3436.5
83	1604.0
84	1604.0
85	1604.0
86	1041.0
87	478.0
88	478.0
89	478.0
90	310.5
91	143.0
92	143.0
93	143.0
94	88.5
95	34.0
96	34.0
97	34.0
98	21.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03274429087949412
2	0.01706083989419149
3	0.023259089984191333
4	0.023728654384948894
5	0.023509524331262032
6	0.008139116279797774
7	0.0011269545618181533
8	5.634772809090767E-4
9	0.0020347790699494434
10	0.0010330416816666404
11	0.0011895631485858286
12	0.0013773889088888542
13	0.0025982563508585204
14	7.513030412121022E-4
15	0.0020973876567171187
16	0.0024104305905554946
17	0.002222604830252469
18	0.002066083363333281
19	5.008686941414015E-4
20	4.0695581398988875E-4
21	0.07941899231479597
22	0.07597552004257384
23	0.0776033432985334
24	0.0012521717353535036
25	0.2300865563712063
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3194450.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.1243504536682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.00730527342448	62.16103981935848
2	10.458946300347495	15.296073092854131
3	2.409809157487379	5.286471880756979
4	0.8426606656342545	2.464760553094421
5	0.3701505857565446	1.3533510576746066
6	0.20367258003043762	0.8936055071969101
7	0.1312760099448194	0.6719631070154942
8	0.09239176637151963	0.540487032254755
9	0.07383451075461973	0.4859190575996352
>10	0.3693776093216074	4.878768364795759
>50	0.02644486795465701	1.3084468347406335
>100	0.011852902215556235	1.4971038881557603
>500	0.001203350589839939	0.6660534694583288
>1k	9.454897468872904E-4	1.659822590613319
>5k	4.2976806676695016E-5	0.16605768268707927
>10k+	8.595361335339003E-5	0.6700760617437366
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	11217	0.3511402588865063	No Hit
CCCATGTACTCTGCGTTGATACCAC	10105	0.3163298846436789	No Hit
GAGTACATGGGAAGCAGTGGTATCA	5284	0.16541188624019784	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4469	0.1398988871323702	No Hit
CATGTACTCTGCGTTGATACCACTG	3793	0.11873718480489598	No Hit
ATTCCATTCCATTCCATTCCATTCC	3538	0.11075458999201741	No Hit
GCGTTGATACCACTGCTTCCCATGT	3450	0.10799981217423969	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3393	0.10621546745136096	No Hit
GTATCAACGCAGAGTACATGGGAAG	3253	0.10183286637762369	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.130429338383759E-5	0.0
11	0.0	0.0	0.0	3.130429338383759E-5	0.0
12	0.0	0.0	0.0	3.130429338383759E-5	0.0
13	0.0	0.0	0.0	3.130429338383759E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGAC	70	1.0916206E-4	12.214256	3
AGTCCGC	60	0.0058979657	11.078275	6
CTGGACG	140	9.7132215E-8	10.17759	4
TATCGCG	75	0.0026589292	10.128709	7
GCCGTTC	75	0.0026589292	10.128709	8
GTATTAG	475	0.0	10.002168	1
GTCTTAA	325	0.0	9.648246	1
CTACGTC	90	0.0011151736	9.499084	4
TCGCGCG	90	0.001118692	9.495813	9
GTCTAGG	325	0.0	9.355874	1
GTCCTGG	605	0.0	9.266472	1
TACACTG	780	0.0	9.132304	5
GTATAGA	470	0.0	9.097717	1
TCTAGAC	230	4.1836756E-11	9.086935	3
TGCTCGG	115	1.11661226E-4	9.082952	10
GTTCTAG	370	0.0	8.988435	1
CTATCGG	245	1.6370905E-11	8.914296	12
ACTATCG	225	2.6557245E-10	8.862619	11
TTACACT	515	0.0	8.853515	4
TAGACTG	420	0.0	8.819197	5
>>END_MODULE