Basic Statistics
| Measure | Value |
|---|---|
| Filename | SRR1294728_1.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 3194450 |
| Sequences flagged as poor quality | 0 |
| Sequence length | 25 |
| %GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
| Sequence | Count | Percentage | Possible Source |
|---|---|---|---|
| GTATCAACGCAGAGTACTTTTTTTT | 12782 | 0.4001314780322121 | No Hit |
| GTACATGGGAAGCAGTGGTATCAAC | 11522 | 0.36068806836857675 | No Hit |
| CCCATGTACTCTGCGTTGATACCAC | 11001 | 0.3443785315155974 | No Hit |
| GGTATCAACGCAGAGTACTTTTTTT | 9809 | 0.30706381380206293 | No Hit |
| TATCAACGCAGAGTACTTTTTTTTT | 8537 | 0.26724475261782155 | No Hit |
| GAGTACATGGGAAGCAGTGGTATCA | 5814 | 0.18200316173363176 | No Hit |
| ACGCAGAGTACTTTTTTTTTTTTTT | 5325 | 0.16669536226893517 | No Hit |
| CATGTACTCTGCGTTGATACCACTG | 4362 | 0.13654932774029957 | No Hit |
| ATTCCATTCCATTCCATTCCATTCC | 4118 | 0.1289110801546432 | No Hit |
| ACGCAGAGTACATGGGAAGCAGTGG | 3912 | 0.12246239571757267 | No Hit |
| GCGTTGATACCACTGCTTCCCATGT | 3756 | 0.117578925949694 | No Hit |
| GTATCAACGCAGAGTACATGGGAAG | 3528 | 0.11044154705817902 | No Hit |
| GAATGGAATGGAATGGAATGGAATG | 3421 | 0.1070919876661084 | No Hit |
Adapter Content
Kmer Content
| Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| TACCGTC | 40 | 0.005301949 | 14.241384 | 7 |
| CCGGTCG | 40 | 0.00530305 | 14.240937 | 9 |
| AGCGTAT | 55 | 1.9720438E-4 | 13.809178 | 10 |
| CCGCGTG | 60 | 0.0059058587 | 11.076284 | 9 |
| CGTGCAA | 75 | 0.002663241 | 10.12673 | 10 |
| TTGGACC | 205 | 4.0017767E-11 | 9.747794 | 4 |
| TAGACTG | 415 | 0.0 | 9.630352 | 5 |
| TCTATAC | 450 | 0.0 | 9.515705 | 3 |
| CTACGCA | 90 | 0.0010974841 | 9.515704 | 4 |
| CGGACTG | 90 | 0.0010974841 | 9.515704 | 5 |
| GCGCAGA | 80 | 0.0045420057 | 9.49381 | 11 |
| TCTAGAC | 260 | 0.0 | 9.149715 | 3 |
| CTAATAC | 460 | 0.0 | 9.101978 | 3 |
| ATCGCGC | 105 | 4.5220088E-4 | 9.042148 | 8 |
| TATTCCG | 160 | 6.5875065E-7 | 8.920973 | 5 |
| TCAGTAC | 320 | 0.0 | 8.920973 | 3 |
| AGGACCG | 120 | 1.7333939E-4 | 8.722729 | 5 |
| GTCTAGC | 230 | 3.8926373E-10 | 8.6915245 | 1 |
| CTAGACT | 340 | 0.0 | 8.676083 | 4 |
| TCAGAAC | 385 | 0.0 | 8.6506405 | 3 |