FastQCFastQC Report
Wed 25 May 2016
SRR1294728_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294728_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3194450
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT127820.4001314780322121No Hit
GTACATGGGAAGCAGTGGTATCAAC115220.36068806836857675No Hit
CCCATGTACTCTGCGTTGATACCAC110010.3443785315155974No Hit
GGTATCAACGCAGAGTACTTTTTTT98090.30706381380206293No Hit
TATCAACGCAGAGTACTTTTTTTTT85370.26724475261782155No Hit
GAGTACATGGGAAGCAGTGGTATCA58140.18200316173363176No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53250.16669536226893517No Hit
CATGTACTCTGCGTTGATACCACTG43620.13654932774029957No Hit
ATTCCATTCCATTCCATTCCATTCC41180.1289110801546432No Hit
ACGCAGAGTACATGGGAAGCAGTGG39120.12246239571757267No Hit
GCGTTGATACCACTGCTTCCCATGT37560.117578925949694No Hit
GTATCAACGCAGAGTACATGGGAAG35280.11044154705817902No Hit
GAATGGAATGGAATGGAATGGAATG34210.1070919876661084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC400.00530194914.2413847
CCGGTCG400.0053030514.2409379
AGCGTAT551.9720438E-413.80917810
CCGCGTG600.005905858711.0762849
CGTGCAA750.00266324110.1267310
TTGGACC2054.0017767E-119.7477944
TAGACTG4150.09.6303525
TCTATAC4500.09.5157053
CTACGCA900.00109748419.5157044
CGGACTG900.00109748419.5157045
GCGCAGA800.00454200579.4938111
TCTAGAC2600.09.1497153
CTAATAC4600.09.1019783
ATCGCGC1054.5220088E-49.0421488
TATTCCG1606.5875065E-78.9209735
TCAGTAC3200.08.9209733
AGGACCG1201.7333939E-48.7227295
GTCTAGC2303.8926373E-108.69152451
CTAGACT3400.08.6760834
TCAGAAC3850.08.65064053