Basic Statistics
Measure | Value |
---|---|
Filename | SRR1294728_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 3194450 |
Sequences flagged as poor quality | 0 |
Sequence length | 25 |
%GC | 45 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GTATCAACGCAGAGTACTTTTTTTT | 12782 | 0.4001314780322121 | No Hit |
GTACATGGGAAGCAGTGGTATCAAC | 11522 | 0.36068806836857675 | No Hit |
CCCATGTACTCTGCGTTGATACCAC | 11001 | 0.3443785315155974 | No Hit |
GGTATCAACGCAGAGTACTTTTTTT | 9809 | 0.30706381380206293 | No Hit |
TATCAACGCAGAGTACTTTTTTTTT | 8537 | 0.26724475261782155 | No Hit |
GAGTACATGGGAAGCAGTGGTATCA | 5814 | 0.18200316173363176 | No Hit |
ACGCAGAGTACTTTTTTTTTTTTTT | 5325 | 0.16669536226893517 | No Hit |
CATGTACTCTGCGTTGATACCACTG | 4362 | 0.13654932774029957 | No Hit |
ATTCCATTCCATTCCATTCCATTCC | 4118 | 0.1289110801546432 | No Hit |
ACGCAGAGTACATGGGAAGCAGTGG | 3912 | 0.12246239571757267 | No Hit |
GCGTTGATACCACTGCTTCCCATGT | 3756 | 0.117578925949694 | No Hit |
GTATCAACGCAGAGTACATGGGAAG | 3528 | 0.11044154705817902 | No Hit |
GAATGGAATGGAATGGAATGGAATG | 3421 | 0.1070919876661084 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TACCGTC | 40 | 0.005301949 | 14.241384 | 7 |
CCGGTCG | 40 | 0.00530305 | 14.240937 | 9 |
AGCGTAT | 55 | 1.9720438E-4 | 13.809178 | 10 |
CCGCGTG | 60 | 0.0059058587 | 11.076284 | 9 |
CGTGCAA | 75 | 0.002663241 | 10.12673 | 10 |
TTGGACC | 205 | 4.0017767E-11 | 9.747794 | 4 |
TAGACTG | 415 | 0.0 | 9.630352 | 5 |
TCTATAC | 450 | 0.0 | 9.515705 | 3 |
CTACGCA | 90 | 0.0010974841 | 9.515704 | 4 |
CGGACTG | 90 | 0.0010974841 | 9.515704 | 5 |
GCGCAGA | 80 | 0.0045420057 | 9.49381 | 11 |
TCTAGAC | 260 | 0.0 | 9.149715 | 3 |
CTAATAC | 460 | 0.0 | 9.101978 | 3 |
ATCGCGC | 105 | 4.5220088E-4 | 9.042148 | 8 |
TATTCCG | 160 | 6.5875065E-7 | 8.920973 | 5 |
TCAGTAC | 320 | 0.0 | 8.920973 | 3 |
AGGACCG | 120 | 1.7333939E-4 | 8.722729 | 5 |
GTCTAGC | 230 | 3.8926373E-10 | 8.6915245 | 1 |
CTAGACT | 340 | 0.0 | 8.676083 | 4 |
TCAGAAC | 385 | 0.0 | 8.6506405 | 3 |