FastQCFastQC Report
Wed 25 May 2016
SRR1294727_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294727_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458198
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC73460.21242277047178906No Hit
CCCATGTACTCTGCGTTGATACCAC65960.19073517479334612No Hit
GTATCAACGCAGAGTACTTTTTTTT49200.14227062765058565No Hit
GGTATCAACGCAGAGTACTTTTTTT37620.10878497992306976No Hit
GAGTACATGGGAAGCAGTGGTATCA34880.10086177830187862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACC604.0988575E-412.6649894
GGTCGTT550.003079461912.08581211
GCGTCAG600.0058655811.0865151
ACCGTTC600.005897874511.078348
CCGTTCA700.001497822810.8522529
TAGGACG803.7778125E-410.6860854
CTGATCG1002.4126728E-510.44529159
ACCGCGT750.002658894310.1287688
TACACCG951.648308E-49.9980965
TATACTG8850.09.9811515
CTAGTAC2151.0913936E-119.7202043
TAGACAG5050.09.5922345
GTCGTGA800.0045071249.5027281
CTAAGAC2900.09.4992913
CGGAAAT900.00111869329.49585814
GTCTTAG3600.09.2387631
GTAGGAC2800.09.160033
TGTATCG1054.5035474E-49.04589755
GTCTCGC2002.6466296E-99.0275921
ATACCGT950.0018316498.9962056
Produced by FastQC (version 0.11.5)