##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294727_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3458198
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.646613930145122	33.0	33.0	33.0	27.0	33.0
2	30.655191229651976	33.0	33.0	33.0	27.0	33.0
3	30.58751841276873	33.0	33.0	33.0	27.0	33.0
4	33.95929643126276	37.0	33.0	37.0	27.0	37.0
5	34.21814713905913	37.0	33.0	37.0	27.0	37.0
6	34.332172131266056	37.0	37.0	37.0	27.0	37.0
7	34.32328397622114	37.0	37.0	37.0	27.0	37.0
8	34.259012352676166	37.0	37.0	37.0	27.0	37.0
9	33.96305734952134	37.0	33.0	37.0	27.0	37.0
10	34.058050753600575	37.0	33.0	37.0	27.0	37.0
11	34.291011677179846	37.0	37.0	37.0	27.0	37.0
12	34.20911844839422	37.0	37.0	37.0	27.0	37.0
13	34.12472941109792	37.0	37.0	37.0	27.0	37.0
14	35.1113603096179	37.0	33.0	40.0	27.0	40.0
15	35.43532267383186	37.0	33.0	40.0	27.0	40.0
16	35.446191340114126	37.0	33.0	40.0	27.0	40.0
17	35.37146137959712	37.0	33.0	40.0	27.0	40.0
18	35.382663745684894	37.0	33.0	40.0	27.0	40.0
19	35.47163204651671	37.0	33.0	40.0	27.0	40.0
20	35.35812726743813	37.0	33.0	40.0	27.0	40.0
21	35.305088083446925	37.0	33.0	40.0	27.0	40.0
22	35.14392033076186	37.0	33.0	40.0	27.0	40.0
23	34.75797742061039	37.0	33.0	40.0	22.0	40.0
24	35.083000452837	37.0	33.0	40.0	27.0	40.0
25	33.77496806140076	37.0	33.0	40.0	15.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4639310943176156
1101	2	0.6440973071684226
1101	3	0.35356135156719404
1101	4	0.5629578314055976
1101	5	0.4777743230987568
1101	6	0.25946499264958334
1101	7	0.41355791791812635
1101	8	0.4699532649011502
1101	9	0.7330172885088473
1101	10	0.6926392716778906
1101	11	0.356117834626545
1101	12	0.4403599914443319
1101	13	0.5182698572799751
1101	14	1.0884117901205101
1101	15	0.9090696799509885
1101	16	0.874882777365805
1101	17	0.8659136911008787
1101	18	0.9219173736075135
1101	19	0.8238314395335635
1101	20	1.0295484697375485
1101	21	0.8938711876447769
1101	22	0.9058365323427466
1101	23	1.2836558429483063
1101	24	1.1536365537417907
1101	25	0.9673491373796281
1102	1	0.3861863136526047
1102	2	0.6299045670791763
1102	3	0.3863141947683957
1102	4	0.3416720037188554
1102	5	0.2951040657793058
1102	6	0.24175442102219336
1102	7	0.2964603788830402
1102	8	0.33419625449705137
1102	9	0.5740623659851707
1102	10	0.6598494958621117
1102	11	0.444385563217061
1102	12	0.39686134371493154
1102	13	0.46527521608845746
1102	14	1.0196007160631382
1102	15	0.7759721838683689
1102	16	0.5151941729972478
1102	17	0.6908133588537737
1102	18	0.8006889541721094
1102	19	0.7023296455358832
1102	20	0.9019744739426585
1102	21	0.9150833616107334
1102	22	0.9529250464785832
1102	23	1.1773191580751075
1102	24	1.066086204582156
1102	25	0.8703372868913135
1103	1	0.25531406527444744
1103	2	0.49227598061149536
1103	3	0.18591293878030157
1103	4	-0.013032423739829824
1103	5	0.17567125769822667
1103	6	0.13639305473411412
1103	7	0.19361872626414822
1103	8	0.24761019130354356
1103	9	0.4617186705613392
1103	10	0.49723820470779856
1103	11	0.2127080192569295
1103	12	0.3078142663358747
1103	13	0.28633269062829214
1103	14	0.9355862249573832
1103	15	0.6478574351454043
1103	16	0.37961472141791575
1103	17	0.47279848339025676
1103	18	0.4294621357501782
1103	19	0.5639770463479081
1103	20	0.7673572928672172
1103	21	0.7226695208936889
1103	22	0.6219976449620077
1103	23	1.1899771336010758
1103	24	0.9104668750977467
1103	25	0.6444810568277077
1104	1	0.15948108051133403
1104	2	0.43988062905199854
1104	3	0.16514240554294446
1104	4	-0.017069747872746177
1104	5	-0.049813990350465076
1104	6	-0.12548242617787508
1104	7	0.032541287291515175
1104	8	0.17613345480332043
1104	9	0.4214137260123536
1104	10	0.3266453350295464
1104	11	0.2322333141166908
1104	12	0.19111640454986656
1104	13	0.13520804222456206
1104	14	0.6415878533548707
1104	15	0.4452170961788724
1104	16	0.37697876447698775
1104	17	0.37329834840548415
1104	18	0.19126725144331402
1104	19	0.3971966155549538
1104	20	0.5040428681275841
1104	21	0.5241390929352576
1104	22	0.6348033104001729
1104	23	1.0413562535861445
1104	24	0.8695982810139711
1104	25	0.6352172095501842
1105	1	0.190047664770713
1105	2	0.26731151930506414
1105	3	0.32019066482992287
1105	4	0.2851783146183351
1105	5	0.21593349740440715
1105	6	0.1838290859334819
1105	7	0.21190656664084173
1105	8	0.29497105119994416
1105	9	-1.6232063256226326
1105	10	-0.26673351234379794
1105	11	0.021985951603731735
1105	12	0.15184737742968224
1105	13	0.22942714764713656
1105	14	-1.6185382624129474
1105	15	-0.3123099131309317
1105	16	-0.18563067256882704
1105	17	0.0661235053020306
1105	18	0.06860476389643111
1105	19	0.2560695490259093
1105	20	0.30746898272828105
1105	21	0.48829282117915085
1105	22	0.49007396761049904
1105	23	0.9827789104924207
1105	24	0.6795263140455177
1105	25	0.328651031691102
1106	1	0.04716772223626364
1106	2	0.04584750661599202
1106	3	0.04940377136794538
1106	4	-1.111455930727466
1106	5	-0.22747572644954062
1106	6	-0.1420544779488324
1106	7	-0.002517383573838572
1106	8	0.23669541431873853
1106	9	0.4112901729220795
1106	10	0.33784744978080283
1106	11	0.35807052353099067
1106	12	0.14098766328910983
1106	13	0.12234083772156623
1106	14	0.6086968386402063
1106	15	0.42353421497472965
1106	16	0.19082124873191475
1106	17	0.11949720098921546
1106	18	0.24137251954292793
1106	19	0.16888792302206923
1106	20	0.4048452891133181
1106	21	0.5829727657266162
1106	22	0.576525470027299
1106	23	0.7843428943480717
1106	24	0.5560218259187266
1106	25	0.48273615660436775
1107	1	0.4022947593678303
1107	2	0.48677921578085304
1107	3	0.41489987232378667
1107	4	0.6375305676209493
1107	5	0.5663536622624434
1107	6	0.3574558204703493
1107	7	0.32599649957570165
1107	8	0.3242804242174273
1107	9	0.42243193891326314
1107	10	0.5705980027840383
1107	11	0.3153683262378024
1107	12	0.365793479141054
1107	13	0.2743886630481711
1107	14	0.7487284067521145
1107	15	0.613975336335649
1107	16	0.6222071953600121
1107	17	0.7436368471346668
1107	18	0.7248197705294217
1107	19	0.5529440683802136
1107	20	0.7439826243264562
1107	21	0.6065041420767159
1107	22	0.6642731229708403
1107	23	0.8659218012922807
1107	24	0.8632275711429145
1107	25	0.6597867418830035
1108	1	0.5299060400275302
1108	2	0.3965922823162309
1108	3	0.5697317126306665
1108	4	0.6931087899100987
1108	5	0.5664043876008051
1108	6	0.5273203486409557
1108	7	0.5252074547458108
1108	8	0.6851512739483923
1108	9	0.2616594379786008
1108	10	0.5391259482683282
1108	11	0.4166429232801505
1108	12	0.5214874103639744
1108	13	0.5366797542996977
1108	14	-1.042869358662827
1108	15	0.189166616629997
1108	16	0.44044619628589743
1108	17	0.6102920443053392
1108	18	0.7298112397586678
1108	19	0.7207638337781219
1108	20	0.7444311178618506
1108	21	0.8342518774941468
1108	22	0.9428749087985508
1108	23	1.122738713160068
1108	24	1.0356399583105116
1108	25	0.921951719310627
1109	1	0.5454549416757857
1109	2	0.4504546116594632
1109	3	0.5437962735228403
1109	4	0.7758108486045501
1109	5	0.6991569030647113
1109	6	0.5543611859283502
1109	7	0.4681291780968664
1109	8	0.5202282026504079
1109	9	0.7362024500516924
1109	10	0.7344099688694357
1109	11	0.5741332682896783
1109	12	0.60648273333166
1109	13	0.5133904537431206
1109	14	0.8234453310624446
1109	15	0.6586587619378577
1109	16	0.653048344918254
1109	17	0.5400533885905929
1109	18	0.6731036167066122
1109	19	0.5983612591720373
1109	20	0.7942597516792347
1109	21	0.7630915097455144
1109	22	0.9039924424514325
1109	23	1.3760916633433808
1109	24	1.0782001654961633
1109	25	0.8927372849546344
1110	1	0.645865817933629
1110	2	0.5745699407549409
1110	3	0.6351968474755303
1110	4	0.8875673612014552
1110	5	0.8869946263445314
1110	6	0.7029522132801489
1110	7	0.5976100028532585
1110	8	0.52812833294049
1110	9	0.5795526114884808
1110	10	0.7096236456919769
1110	11	0.4866973726574031
1110	12	0.515154284068295
1110	13	0.42894433123279896
1110	14	0.8652174148645955
1110	15	0.7264546819591047
1110	16	0.662593043616539
1110	17	0.6788428886228459
1110	18	0.7998516290588213
1110	19	0.7242809604173033
1110	20	0.828032566767611
1110	21	0.9294546351658894
1110	22	1.0002478930420082
1110	23	1.1733607201046894
1110	24	1.0328119730145247
1110	25	0.9379924562601829
1111	1	0.3466013823250087
1111	2	0.1560288632794311
1111	3	0.401110132532299
1111	4	0.637335957966016
1111	5	0.5321636405242884
1111	6	0.4118762200441992
1111	7	0.24816921171490947
1111	8	0.4040266519823916
1111	9	0.32504661416977854
1111	10	0.3698909417878369
1111	11	0.29444701020499053
1111	12	0.23662032856597648
1111	13	0.19908702100099873
1111	14	-1.677382621985572
1111	15	-0.45086979122335435
1111	16	0.08176879139728754
1111	17	0.19996672458388787
1111	18	0.3395394192142476
1111	19	0.4286447185795552
1111	20	0.3850230148561309
1111	21	0.6507012708411963
1111	22	0.3218456853905636
1111	23	-1.7569302510556142
1111	24	-0.18241154435503404
1111	25	0.23983669221318848
1112	1	-0.12054596365972259
1112	2	-0.21750524964367557
1112	3	0.08613944025221798
1112	4	0.24631205334373618
1112	5	-0.67948521599034
1112	6	-0.9011094769457344
1112	7	-1.0355666266109225
1112	8	-0.7633208995371987
1112	9	-0.7217332780435939
1112	10	-0.9375495836321335
1112	11	-0.8749021926909677
1112	12	-0.9035832405039059
1112	13	-0.8188109380349076
1112	14	-0.6011628177063955
1112	15	-0.9784974061317868
1112	16	-0.8400719025226095
1112	17	-0.860865062338533
1112	18	-0.8774164516972291
1112	19	-0.847581959442671
1112	20	-0.9518679569826816
1112	21	-2.034146125071395
1112	22	-1.9188081604608982
1112	23	-1.5267978311304446
1112	24	-1.8443482761493826
1112	25	-2.0432567370260593
1113	1	0.1771973681102068
1113	2	-0.08659157720467192
1113	3	0.106214606325711
1113	4	0.41588000169618766
1113	5	0.14809607342050413
1113	6	-0.0220496046544838
1113	7	0.05014262292388594
1113	8	0.2416385592180248
1113	9	0.295728119640728
1113	10	-0.015807520979429057
1113	11	-0.01600436275880668
1113	12	-0.12565098193506685
1113	13	-0.09503773602762067
1113	14	0.13344870673637388
1113	15	-0.03682275383618361
1113	16	0.107106580115925
1113	17	0.17979162918934577
1113	18	0.19911983923103094
1113	19	-0.03489578393050152
1113	20	-0.6489315140055965
1113	21	-0.5582029165345901
1113	22	0.02378749082001974
1113	23	-0.19908343768571513
1113	24	0.12722999680891434
1113	25	-0.928793245604858
1114	1	-0.8661339419927643
1114	2	-0.7439773796454254
1114	3	-0.5283983895573208
1114	4	-1.4517484044581153
1114	5	-0.6824556860235376
1114	6	-0.5385373993640457
1114	7	-0.4322117428087253
1114	8	-0.3890416753540791
1114	9	-0.40109724798942636
1114	10	-0.5017641564086475
1114	11	-0.5666722433636906
1114	12	-0.4950562441733055
1114	13	-0.616818975777349
1114	14	-0.5337156745996339
1114	15	-1.445909602532275
1114	16	-0.879561680611026
1114	17	-0.8260930895969665
1114	18	-0.8314615329483246
1114	19	-0.8105384507933593
1114	20	-2.0790739678161643
1114	21	-6.659866900888275
1114	22	-7.87285324171199
1114	23	-8.020769524298366
1114	24	-6.119553588048738
1114	25	-7.322131453084069
1115	1	-1.072657678918258
1115	2	-1.0857448698471721
1115	3	-0.7956666622402935
1115	4	-0.6163937449156194
1115	5	-0.6510339487449528
1115	6	-0.8080684680411849
1115	7	-0.9656423006670494
1115	8	-0.7012174043292276
1115	9	-2.6635374953043787
1115	10	-1.6681166933174651
1115	11	-1.3000014246985785
1115	12	-1.0643955461986323
1115	13	-1.3026789026715377
1115	14	-1.0278760204205284
1115	15	-1.8712330942481614
1115	16	-1.1741478155991842
1115	17	-2.9814771968215865
1115	18	-2.774403427995942
1115	19	-2.0040800172072792
1115	20	-1.8015162919935435
1115	21	-1.0693137893074862
1115	22	-0.7230154836409568
1115	23	-0.17956041746379725
1115	24	-1.1756324018702529
1115	25	-0.9859277918630625
1116	1	-1.479750623384117
1116	2	-0.7207956916617846
1116	3	-0.5192072903527993
1116	4	-0.06225675564213162
1116	5	-0.17808249503242735
1116	6	-0.1565537361374325
1116	7	-0.5044226566802479
1116	8	-1.6274219766974412
1116	9	-0.652854207537942
1116	10	-2.047172739725241
1116	11	-0.8359170600502495
1116	12	-0.6006699458221689
1116	13	-0.6226032221153375
1116	14	-0.956301955278974
1116	15	-1.027334760675501
1116	16	-0.5292129405523553
1116	17	-0.490783952858898
1116	18	-1.0321351824859661
1116	19	-0.496444447039849
1116	20	-0.952781889479688
1116	21	-0.33084387780623814
1116	22	0.15877388826107364
1116	23	0.30584630355775033
1116	24	0.1423166293514555
1116	25	0.35037165508377655
1201	1	0.29285357526832456
1201	2	0.338869914684512
1201	3	0.33600381556459524
1201	4	0.3155987849693247
1201	5	0.3107213195982226
1201	6	0.215008232321928
1201	7	0.27635750562846795
1201	8	0.30364813492246157
1201	9	0.6935255684392416
1201	10	0.5671601123044923
1201	11	0.4977131122058722
1201	12	0.44461061016497894
1201	13	0.46605219626045624
1201	14	1.078877490914195
1201	15	0.7799791974919614
1201	16	0.8043300433754226
1201	17	0.6553386482642054
1201	18	0.5393429475770688
1201	19	0.6753724193026898
1201	20	0.8141616612172697
1201	21	0.8628387892769425
1201	22	0.8127643657721748
1201	23	0.5653926474100075
1201	24	0.7515538170696061
1201	25	0.6793596733844041
1202	1	-0.46681916460601514
1202	2	-0.12617294297798765
1202	3	-0.27846333928102496
1202	4	-0.39679296116253937
1202	5	-0.3291377717390347
1202	6	-0.444874343268701
1202	7	-0.24100726605882272
1202	8	-0.21485027129557466
1202	9	-0.09664869285992239
1202	10	0.14362582173564675
1202	11	-0.1005372040718342
1202	12	-0.16078310815078112
1202	13	-0.01489033841892251
1202	14	0.08848347130674483
1202	15	0.0470580563294547
1202	16	0.04878507820309608
1202	17	-0.14921421876387342
1202	18	-0.16938022852600199
1202	19	-0.12815625239220907
1202	20	0.1595120548094897
1202	21	0.022400746514890102
1202	22	-0.03807436822449972
1202	23	0.40051585764113184
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2211	7	0.31424095653220263
2211	8	0.4483981698433368
2211	9	0.6012259289497877
2211	10	0.5914611732221147
2211	11	0.3924214562296271
2211	12	0.2508996422743621
2211	13	0.1670473617743582
2211	14	0.5769721443795959
2211	15	0.4468401914057054
2211	16	0.3902490758537027
2211	17	0.46973388294284746
2211	18	0.4408874243380012
2211	19	0.3825082014771155
2211	20	0.5643207912612311
2211	21	0.44013454153063236
2211	22	0.523931764775547
2211	23	0.647369109565382
2211	24	0.6192613814355852
2211	25	0.5855470722744798
2212	1	-0.2525443153782163
2212	2	-0.37938267562424954
2212	3	-0.26024724521614573
2212	4	-0.5820191662423682
2212	5	-0.26887272837819154
2212	6	-0.5870866125155771
2212	7	-0.5436062487876683
2212	8	-0.42146829585229995
2212	9	-0.2678728847411307
2212	10	-0.6327507208875289
2212	11	-0.4410935590892038
2212	12	-0.5135816598928216
2212	13	-0.7512000782723831
2212	14	-0.43130077219950635
2212	15	-0.6267082017872099
2212	16	-0.5544351132193768
2212	17	-0.573494439115187
2212	18	-0.4158628466698744
2212	19	-0.6323899168305687
2212	20	-0.5658927665288545
2212	21	-0.4427503702952791
2212	22	-0.536723398189217
2212	23	-0.028173093537226634
2212	24	-0.17446136911759424
2212	25	-0.8328444165631268
2213	1	-0.16436481440161899
2213	2	-1.4320527144306503
2213	3	-0.33411387823196037
2213	4	-0.08130145862352833
2213	5	-0.02258888648897539
2213	6	-0.03885000511537129
2213	7	-0.037574601929570406
2213	8	0.08000333406244664
2213	9	0.009638211432765331
2213	10	-0.5569107433270446
2213	11	-0.15575668125553932
2213	12	-0.1144104121668974
2213	13	-0.4263207214753706
2213	14	-0.011399535767573354
2213	15	-0.08666896737780405
2213	16	-0.03136355267002955
2213	17	-0.08374439303742065
2213	18	0.012354656816953025
2213	19	0.01102934884065121
2213	20	0.1945652914894893
2213	21	-0.5294683966285447
2213	22	-1.0176420538334412
2213	23	-0.1095989125961907
2213	24	-0.04190702775485988
2213	25	-0.6454294037869133
2214	1	-0.14320767133404289
2214	2	-0.4556237213233594
2214	3	-0.4578169490728534
2214	4	-0.5684117679965013
2214	5	-0.2618426996867598
2214	6	-0.21542944687749355
2214	7	-0.46667711097461506
2214	8	-0.11870996309446724
2214	9	-0.020723664181240053
2214	10	-1.973822945446642
2214	11	-0.7891863950206712
2214	12	-0.5751158353437518
2214	13	-0.2655151880469262
2214	14	-0.793041343893556
2214	15	-0.4297834273320049
2214	16	-0.6299056652626192
2214	17	-0.4237854342746843
2214	18	-0.3431873549288653
2214	19	-1.3704792055718542
2214	20	-2.3621600551571262
2214	21	-1.808450767706141
2214	22	-1.970438342218955
2214	23	-2.5168209862551265
2214	24	-1.5434735237374184
2214	25	-1.9062982497259036
2215	1	-0.243687813319724
2215	2	-0.3024842862101096
2215	3	-0.9263438476024533
2215	4	-0.23905317186819985
2215	5	-0.15200654740476693
2215	6	-0.17458593672809997
2215	7	-0.22493649343092414
2215	8	-0.15414937822628616
2215	9	-0.20244682735899744
2215	10	-0.16738216687613772
2215	11	-0.4932047191664637
2215	12	-0.3140775650302814
2215	13	-0.2644776229603636
2215	14	-0.0997715593403754
2215	15	-0.550833568965075
2215	16	-0.3469254351235733
2215	17	-0.166357631879265
2215	18	-0.15283456516876015
2215	19	-2.1205961557005537
2215	20	-2.402042399801516
2215	21	-3.331188752402717
2215	22	-2.3865357340547746
2215	23	-3.521653259789133
2215	24	-2.559341422983998
2215	25	-1.7191133979998625
2216	1	-1.5396853913122222
2216	2	-1.2432812550144767
2216	3	-0.8144423566820791
2216	4	-0.8722250152800868
2216	5	-2.0023538576828344
2216	6	-1.5588452089448808
2216	7	-1.1177330690345215
2216	8	-5.504686701409671
2216	9	-4.1307497393997785
2216	10	-2.247156014997561
2216	11	-1.126486023937673
2216	12	-1.1813694732520332
2216	13	-1.9329738450587257
2216	14	-3.4576775399854753
2216	15	-2.0444821136955866
2216	16	-2.9900971385942157
2216	17	-2.7294563201519466
2216	18	-2.7542939134316597
2216	19	-1.684386119592304
2216	20	-0.8866485897782468
2216	21	-0.5741988066692016
2216	22	-0.6022687480035032
2216	23	-0.7829437706463551
2216	24	-0.32995813039859456
2216	25	-0.14188480807173676
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	117.0
8	549.0
9	1405.0
10	2775.0
11	5176.0
12	8289.0
13	12277.0
14	15932.0
15	19747.0
16	22075.0
17	23891.0
18	25241.0
19	25974.0
20	27103.0
21	28538.0
22	30212.0
23	32003.0
24	33109.0
25	35431.0
26	38083.0
27	42555.0
28	47862.0
29	56020.0
30	66528.0
31	80469.0
32	104829.0
33	144236.0
34	201904.0
35	308137.0
36	511857.0
37	1505870.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.52180047779254	21.102214570445184	12.9863942803738	23.389590671388476
2	15.50401541846653	20.9175711287826	39.97328260170427	23.6051308510466
3	18.622050626021156	25.764699110997608	29.259594657482303	26.353655605498933
4	12.190904755004427	16.975382289930348	37.71912850998254	33.11458444508269
5	13.19235755154268	38.175307295080216	34.92132586496428	13.711009288412827
6	31.66350346746975	38.23320263146556	16.825029055837813	13.278264845226875
7	26.39617923098811	32.84965496022153	22.952548066134746	17.801617742655615
8	24.439673769086717	37.50111330752387	20.689938360355377	17.369274563034026
9	24.101660263982406	17.3123762898746	22.01145976863586	36.57450367750713
10	14.740506021643679	30.726402698288585	33.26579322374235	21.267298056325387
11	32.802781594032204	25.95177031954267	22.315184284363855	18.93026380206127
12	20.563443980774753	27.90911122992828	31.375317690529737	20.152127098767238
13	26.57622995689263	24.101698355951186	26.49063404305648	22.831437644099708
14	20.299926984608934	23.341733717929255	28.01145112667809	28.346888170783725
15	22.9392294781795	30.955974973714074	23.690099024789163	22.414696523317268
16	21.915854108873827	31.468751888674547	25.436242251193065	21.179151751258562
17	20.185135597033526	30.473200131632446	28.322808328470373	21.018855942863652
18	20.435932396500395	30.124013007123846	29.080117578847375	20.359937017528388
19	20.913739168454214	28.836794782004205	29.469409684730664	20.780056364810914
20	22.220850277413987	28.520621631726893	28.642911072818748	20.615617018040368
21	22.506989181961835	28.78367975365666	27.929089467317255	20.780241597064254
22	21.116425622943876	28.617797338062374	29.272834715852397	20.992942323141357
23	21.39372418962289	28.90145825917598	28.783414219112196	20.921403332088936
24	21.230429254409643	28.18710764889642	28.98186578465594	21.600597312037994
25	21.856699765884525	28.234129387781365	28.065339026916615	21.8438318194175
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3045.0
1	3045.0
2	3490.0
3	3935.0
4	3935.0
5	3935.0
6	5624.5
7	7314.0
8	7314.0
9	7314.0
10	11187.5
11	15061.0
12	15061.0
13	15061.0
14	23926.0
15	32791.0
16	32791.0
17	32791.0
18	50846.5
19	68902.0
20	68902.0
21	68902.0
22	97503.0
23	126104.0
24	126104.0
25	126104.0
26	163275.0
27	200446.0
28	200446.0
29	200446.0
30	239734.5
31	279023.0
32	279023.0
33	279023.0
34	313605.5
35	348188.0
36	348188.0
37	348188.0
38	373153.0
39	398118.0
40	398118.0
41	398118.0
42	399044.0
43	399970.0
44	399970.0
45	399970.0
46	402902.0
47	405834.0
48	405834.0
49	405834.0
50	383905.5
51	361977.0
52	361977.0
53	361977.0
54	329031.0
55	296085.0
56	296085.0
57	296085.0
58	258878.0
59	221671.0
60	221671.0
61	221671.0
62	185207.0
63	148743.0
64	148743.0
65	148743.0
66	114970.5
67	81198.0
68	81198.0
69	81198.0
70	59096.5
71	36995.0
72	36995.0
73	36995.0
74	26203.5
75	15412.0
76	15412.0
77	15412.0
78	10235.0
79	5058.0
80	5058.0
81	5058.0
82	3359.5
83	1661.0
84	1661.0
85	1661.0
86	1082.0
87	503.0
88	503.0
89	503.0
90	309.0
91	115.0
92	115.0
93	115.0
94	72.5
95	30.0
96	30.0
97	30.0
98	24.5
99	19.0
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030333717155582182
2	0.015210233769147978
3	0.021022509410970683
4	0.023393686538480445
5	0.02232376515167726
6	0.0058122756418227065
7	0.0013301725349444998
8	9.83171004089413E-4
9	3.759183250930109E-4
10	1.7350076542754347E-4
11	0.0010699213868031847
12	0.0010699213868031847
13	0.0025735946871752284
14	6.650862674722499E-4
15	0.0021398427736063694
16	0.0024579275102235326
17	0.002429010715985609
18	0.002689261864126924
19	3.4700153085508693E-4
20	2.602511481413152E-4
21	0.08264419793198655
22	0.07720784061525685
23	0.07830667879629796
24	0.0011277549752790326
25	0.22450999046324127
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3458198.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.2923996792658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.27319344393064	64.20423362707774
2	10.449906529992502	15.735970781343301
3	2.397120489976462	5.414548620319957
4	0.7991106847085838	2.4066784424420185
5	0.3499758758007395	1.3175261759445185
6	0.17303932143720968	0.7817127449927602
7	0.11259560608714954	0.5934315362949985
8	0.06905554221835197	0.41594859878180557
9	0.05304971751035818	0.35948164802958305
>10	0.28975398902795196	3.9832583326478175
>50	0.01978650391491191	1.035144685589666
>100	0.011481611366686352	1.4904341194434987
>500	0.0011584104293039998	0.58695816534474
>1k	6.950462391243858E-4	1.2693344380317007
>5k	7.722735990270952E-5	0.40533808371594066
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7346	0.21242277047178906	No Hit
CCCATGTACTCTGCGTTGATACCAC	6596	0.19073517479334612	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4920	0.14227062765058565	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3762	0.10878497992306976	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3488	0.10086177830187862	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	5.7833588475847824E-5	0.0	0.0	0.0
4	0.0	5.7833588475847824E-5	0.0	0.0	0.0
5	0.0	5.7833588475847824E-5	0.0	0.0	0.0
6	0.0	5.7833588475847824E-5	0.0	0.0	0.0
7	0.0	5.7833588475847824E-5	0.0	0.0	0.0
8	0.0	5.7833588475847824E-5	0.0	0.0	0.0
9	0.0	5.7833588475847824E-5	0.0	0.0	0.0
10	0.0	5.7833588475847824E-5	0.0	2.8916794237923912E-5	0.0
11	0.0	5.7833588475847824E-5	0.0	2.8916794237923912E-5	0.0
12	0.0	5.7833588475847824E-5	0.0	5.7833588475847824E-5	0.0
13	0.0	5.7833588475847824E-5	0.0	5.7833588475847824E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACC	60	4.0988575E-4	12.664989	4
GGTCGTT	55	0.0030794619	12.085812	11
GCGTCAG	60	0.00586558	11.086515	1
ACCGTTC	60	0.0058978745	11.07834	8
CCGTTCA	70	0.0014978228	10.852252	9
TAGGACG	80	3.7778125E-4	10.686085	4
CTGATCG	100	2.4126728E-5	10.4452915	9
ACCGCGT	75	0.0026588943	10.128768	8
TACACCG	95	1.648308E-4	9.998096	5
TATACTG	885	0.0	9.981151	5
CTAGTAC	215	1.0913936E-11	9.720204	3
TAGACAG	505	0.0	9.592234	5
GTCGTGA	80	0.004507124	9.502728	1
CTAAGAC	290	0.0	9.499291	3
CGGAAAT	90	0.0011186932	9.495858	14
GTCTTAG	360	0.0	9.238763	1
GTAGGAC	280	0.0	9.16003	3
TGTATCG	105	4.5035474E-4	9.0458975	5
GTCTCGC	200	2.6466296E-9	9.027592	1
ATACCGT	95	0.001831649	8.996205	6
>>END_MODULE