FastQCFastQC Report
Wed 25 May 2016
SRR1294727_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294727_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3458198
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT142330.411572732388371No Hit
GGTATCAACGCAGAGTACTTTTTTT113920.32942011995842924No Hit
TATCAACGCAGAGTACTTTTTTTTT94650.27369745746194984No Hit
GTACATGGGAAGCAGTGGTATCAAC78120.22589799658666163No Hit
CCCATGTACTCTGCGTTGATACCAC73500.21253843764874078No Hit
ACGCAGAGTACTTTTTTTTTTTTTT59570.17225734327531275No Hit
ATTCCATTCCATTCCATTCCATTCC42730.12356146177864888No Hit
GAGTACATGGGAAGCAGTGGTATCA41460.11988902891043256No Hit
GAATGGAATGGAATGGAATGGAATG36090.1043607104046674No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG400.005300364514.2420727
TGTATCG752.046802E-411.4169345
GTCTACG750.002609015410.1519161
CTAGGAC3100.010.1279263
TAGAGCG1054.041701E-59.9671655
GTCTCGC1659.933501E-99.8058281
TAGGACC2550.09.7006634
CCGTACC1401.0383865E-69.5141124
TGCACCG1106.730997E-59.51411155
TCCTATA3350.09.3722462
TAGACTG6350.09.289375
TATTCCG1752.6091584E-89.242285
TCTATAC5150.09.2370023
TAGACAG6100.09.2021745
TGGAGCG1252.7082518E-59.1335485
AAGCGTG1151.1180909E-49.0819027
GTATCGC950.00183196638.9960356
TCTCGCT1701.6484228E-78.9545882
AGACTGT7150.08.6325586
ACAGCGC1107.1875815E-48.6315598
Produced by FastQC (version 0.11.5)