FastQCFastQC Report
Wed 25 May 2016
SRR1294726_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294726_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2633040
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC63080.23957099018624858No Hit
CCCATGTACTCTGCGTTGATACCAC59060.22430346671527968No Hit
GTATCAACGCAGAGTACTTTTTTTT35490.134787166165345No Hit
GAGTACATGGGAAGCAGTGGTATCA31740.12054507337526206No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATGA658.05607E-411.68721313
CTCCGGT600.00589659411.0785047
TAGACAG4350.010.4809775
GTGTAGG3700.09.7588061
GTACTAG1951.6370905E-109.7456361
GTAAGAC2650.09.6781513
TAATACT4450.09.6052914
GTTCTAG2303.6379788E-129.5019961
AGCGTTA800.00453489939.49549910
GTCTTAG2850.09.3352941
GTCTAGA2650.09.3227121
CTAGGAC3700.09.2421993
GGACAGT3500.08.953756
TAAGACA4100.08.8035494
GTGCTAG2950.08.6967411
GTCCTAC2304.0199666E-108.6757351
GTATAGG3300.08.6381781
TGTACTG4650.08.5791865
TATCGGT1000.00291728048.54627313
CTATACT4250.08.4928364
Produced by FastQC (version 0.11.5)