##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294726_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2633040
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.688962947771397	33.0	33.0	33.0	27.0	33.0
2	30.719683711603317	33.0	33.0	33.0	27.0	33.0
3	30.542675386625344	33.0	33.0	33.0	27.0	33.0
4	33.82007945188831	37.0	33.0	37.0	27.0	37.0
5	34.185209491690216	37.0	33.0	37.0	27.0	37.0
6	34.43451979461003	37.0	37.0	37.0	27.0	37.0
7	34.36409131650108	37.0	37.0	37.0	27.0	37.0
8	34.30990300185337	37.0	37.0	37.0	27.0	37.0
9	33.95159169629022	37.0	33.0	37.0	27.0	37.0
10	34.02528104396439	37.0	33.0	37.0	27.0	37.0
11	34.31556679731413	37.0	37.0	37.0	27.0	37.0
12	34.22005172728101	37.0	37.0	37.0	27.0	37.0
13	34.14782608695652	37.0	37.0	37.0	27.0	37.0
14	35.1253847263999	37.0	33.0	40.0	27.0	40.0
15	35.46633700969222	37.0	33.0	40.0	27.0	40.0
16	35.49364650745906	37.0	37.0	40.0	27.0	40.0
17	35.42826428766749	37.0	33.0	40.0	27.0	40.0
18	35.430117658675904	37.0	33.0	40.0	27.0	40.0
19	35.511569896393524	37.0	33.0	40.0	27.0	40.0
20	35.38251754625832	37.0	33.0	40.0	27.0	40.0
21	35.31717520432656	37.0	33.0	40.0	27.0	40.0
22	35.17804666848966	37.0	33.0	40.0	27.0	40.0
23	34.79285350773251	37.0	33.0	40.0	22.0	40.0
24	35.105254002977546	37.0	33.0	40.0	27.0	40.0
25	33.82068445599003	37.0	33.0	40.0	15.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4828810385762239
1101	2	0.7647250798928695
1101	3	0.4839057649026408
1101	4	0.5745401640199432
1101	5	0.4511629986735173
1101	6	0.4490980537635423
1101	7	0.428127016973356
1101	8	0.5727683898099514
1101	9	0.7759224783300809
1101	10	0.8327013574795075
1101	11	0.7059646811626976
1101	12	0.543957663452062
1101	13	0.6460286662475241
1101	14	1.2579820195570548
1101	15	1.0565427254124202
1101	16	0.9741148213877082
1101	17	1.1597374946389039
1101	18	1.0770045285479952
1101	19	0.8581871932041665
1101	20	1.0691242953539515
1101	21	1.1371271846483992
1101	22	1.1391376644882953
1101	23	1.590866932342152
1101	24	1.247081902914367
1101	25	1.1643965949962762
1102	1	0.2728758275288037
1102	2	0.49844055670370935
1102	3	0.3200183235269236
1102	4	0.20637966375938532
1102	5	0.28310671936137055
1102	6	0.19844667283597772
1102	7	0.42682425511822686
1102	8	0.43988668058639746
1102	9	0.5518474392472257
1102	10	0.5914298619088996
1102	11	0.3948651501569671
1102	12	0.48012233255055037
1102	13	0.4222141795356933
1102	14	1.040160237378835
1102	15	0.850445799930398
1102	16	0.5045994487978192
1102	17	0.7571840814028477
1102	18	0.7179633612733696
1102	19	0.7193127794470016
1102	20	0.914356186964163
1102	21	0.9583882581241667
1102	22	1.025797383091735
1102	23	1.2002989281733178
1102	24	1.0052893026017031
1102	25	0.9351105084928832
1103	1	0.2435276519497691
1103	2	0.5542600784014233
1103	3	0.16200823832676647
1103	4	-0.11559327005229392
1103	5	0.13962859033177466
1103	6	0.13929380400142577
1103	7	0.21535731922078583
1103	8	0.3103629644430086
1103	9	0.5899230172012793
1103	10	0.683667204614018
1103	11	0.42313671879711734
1103	12	0.5468122514513709
1103	13	0.3936598838546388
1103	14	1.0377572245091145
1103	15	0.7803858655380296
1103	16	0.3816259431201061
1103	17	0.4838252374031171
1103	18	0.6869303783220886
1103	19	0.6428879800977114
1103	20	0.7872573244045071
1103	21	0.7724360183556058
1103	22	0.633278408720976
1103	23	1.3431666526607913
1103	24	0.9323922127065245
1103	25	0.7521735562890512
1104	1	0.2471791168957047
1104	2	0.4879573281608387
1104	3	0.23985949336435652
1104	4	0.19279943925721454
1104	5	0.07203407116687544
1104	6	0.14194031172748112
1104	7	0.07788403053062609
1104	8	0.3419287330983778
1104	9	0.5226554292351864
1104	10	0.5509462703012531
1104	11	0.45075803857221075
1104	12	0.3477240867941376
1104	13	0.4114366282581372
1104	14	0.91083949954578
1104	15	0.5455730968490471
1104	16	0.5761520419346127
1104	17	0.658101300497492
1104	18	0.634857966771456
1104	19	0.7713603847144341
1104	20	0.8257916062826283
1104	21	0.7797949579507488
1104	22	0.9610071384287835
1104	23	1.2828539858792567
1104	24	0.9589854622306078
1104	25	0.8715979315017961
1105	1	0.09815544550783528
1105	2	0.14583273593223822
1105	3	0.24473365201323816
1105	4	0.1174541239828173
1105	5	0.06949079062015073
1105	6	0.12585179436364058
1105	7	0.13802695698724676
1105	8	0.1447089339903087
1105	9	-1.8488909018545456
1105	10	-0.3511635595703311
1105	11	-0.17631578869789166
1105	12	0.03408568195456496
1105	13	0.07532472855957906
1105	14	-1.7051133951149993
1105	15	-0.511379405525318
1105	16	-0.25240744107318847
1105	17	-0.04423615872536857
1105	18	-0.11651697128858984
1105	19	0.04258424088984469
1105	20	0.31019693105448454
1105	21	0.5021019891684944
1105	22	0.5094542520266714
1105	23	0.9387346688705449
1105	24	0.6074201878365884
1105	25	0.2575522714282954
1106	1	0.07997297907539291
1106	2	0.21209237128717362
1106	3	0.1663970841428899
1106	4	-1.0938043775175288
1106	5	-0.23168655515796388
1106	6	-0.06884703394378988
1106	7	0.03624362535629899
1106	8	0.08343575340935416
1106	9	0.38417165544043996
1106	10	0.4201416965683862
1106	11	0.3327407864702465
1106	12	0.08725228405896956
1106	13	0.20807867183601303
1106	14	0.5862792031593571
1106	15	0.46420963674978566
1106	16	0.15588402315385963
1106	17	0.06594502031637006
1106	18	0.28373273368437424
1106	19	0.22463798880924202
1106	20	0.4093568508045564
1106	21	0.5322410003830527
1106	22	0.6705188419085744
1106	23	0.8455713944526835
1106	24	0.5510609981218337
1106	25	0.6081333638730939
1107	1	0.46971527228032883
1107	2	0.4266609772302665
1107	3	0.35159728596052986
1107	4	0.5056276398647981
1107	5	0.5232596249609642
1107	6	0.4254334355100582
1107	7	0.36448595162977426
1107	8	0.34805398428439815
1107	9	0.5091479600054711
1107	10	0.5167593487992548
1107	11	0.3986146486746236
1107	12	0.338097412023167
1107	13	0.34730917682471585
1107	14	0.9359132261985152
1107	15	0.6229607562692294
1107	16	0.6130983070827938
1107	17	0.6393424734414381
1107	18	0.589743046203111
1107	19	0.5441243403492919
1107	20	0.7762210218248811
1107	21	0.7362646124277887
1107	22	0.7010042770408162
1107	23	1.01124102799794
1107	24	0.8894645462547004
1107	25	0.697063188446144
1108	1	0.7056316136209837
1108	2	0.5100534957470728
1108	3	0.7184463031924828
1108	4	0.9526082155045827
1108	5	0.8633911693687537
1108	6	0.6668176338010667
1108	7	0.6389796225601572
1108	8	0.7493704083653157
1108	9	0.4126591008900107
1108	10	0.6915252505735481
1108	11	0.503193362868636
1108	12	0.5259886344859552
1108	13	0.5740225896363
1108	14	-0.7012643090518509
1108	15	0.33265606632469513
1108	16	0.603130766183412
1108	17	0.6967864015176133
1108	18	0.8374897742179641
1108	19	0.6760909894459033
1108	20	0.7969639061278215
1108	21	0.8635586770393857
1108	22	0.9585994755859275
1108	23	1.2799310509370088
1108	24	1.102673937203818
1108	25	1.0752003706714035
1109	1	0.5011394710241674
1109	2	0.43874642844244605
1109	3	0.44369055456152395
1109	4	0.6129806721518278
1109	5	0.6062805456261344
1109	6	0.29456544342159674
1109	7	0.40295648705176035
1109	8	0.4961080782122238
1109	9	0.5626367247092645
1109	10	0.6210980205458227
1109	11	0.3789557178841534
1109	12	0.46521715057370017
1109	13	0.3475374401078355
1109	14	0.7629035573486078
1109	15	0.501755467462047
1109	16	0.485541040623616
1109	17	0.5120085809049684
1109	18	0.5926897938739017
1109	19	0.49642681350653106
1109	20	0.7571538433399638
1109	21	0.6882428511418652
1109	22	0.789339024447024
1109	23	1.2886740243775066
1109	24	0.9944177407567452
1109	25	0.8705110836847467
1110	1	0.491803098828516
1110	2	0.3383032632364653
1110	3	0.563693523770116
1110	4	0.8031481139820897
1110	5	0.6702219284809132
1110	6	0.42011346094221125
1110	7	0.501567766774393
1110	8	0.5060038260628374
1110	9	0.6887750518883635
1110	10	0.7400643303396706
1110	11	0.3855225567804865
1110	12	0.38980004997170425
1110	13	0.2851272619433587
1110	14	0.875019552000154
1110	15	0.6451915994833612
1110	16	0.5803339932993197
1110	17	0.6482577697419103
1110	18	0.7345735288489124
1110	19	0.6279646909405372
1110	20	0.778365112956358
1110	21	0.8246219855811034
1110	22	0.832838784808196
1110	23	1.1193498343684425
1110	24	0.9945485978582269
1110	25	0.8942893059069945
1111	1	0.19371340000785509
1111	2	0.12965738249951286
1111	3	0.31767955581556606
1111	4	0.625586898503208
1111	5	0.5617817120264945
1111	6	0.4068119366069354
1111	7	0.36720447182189275
1111	8	0.38281452047343834
1111	9	0.30166826387723944
1111	10	0.3369049843879779
1111	11	0.21058468097780292
1111	12	0.1497538467855648
1111	13	0.2505192900113613
1111	14	-1.7811381795039836
1111	15	-0.5502681768188964
1111	16	-0.010791751976391595
1111	17	0.17166731433471938
1111	18	0.27957463075945554
1111	19	0.3084692514851852
1111	20	0.43787134254871773
1111	21	0.5071327168247919
1111	22	0.27282742665336457
1111	23	-1.8344266695523146
1111	24	-0.404203050879552
1111	25	-0.030416904487246654
1112	1	-0.07813787081390089
1112	2	-0.2941796654745623
1112	3	0.0828280997904649
1112	4	0.33812551724514606
1112	5	-0.6341707938900782
1112	6	-0.8980718563062453
1112	7	-0.9506602169499772
1112	8	-0.8142921419124036
1112	9	-0.7284596417805673
1112	10	-1.0824418437965093
1112	11	-0.9137192844885007
1112	12	-0.923323611147957
1112	13	-0.9176195509745853
1112	14	-0.5845866465137561
1112	15	-0.9853620954204203
1112	16	-0.8635469240520877
1112	17	-1.0001477168710835
1112	18	-1.1589955353532062
1112	19	-0.9696762093987772
1112	20	-1.1869070062192932
1112	21	-1.9199477927984105
1112	22	-1.8590657103772443
1112	23	-1.599219225787806
1112	24	-1.7910561448968423
1112	25	-1.912632545261971
1113	1	0.21082581575040749
1113	2	-0.04255908399576214
1113	3	0.11422228249528033
1113	4	0.4257538145847093
1113	5	0.22114819834705202
1113	6	0.1312277303216831
1113	7	0.1259167238459895
1113	8	0.15689967965126073
1113	9	0.43912653485686093
1113	10	0.06401190021367142
1113	11	-0.06496754904662794
1113	12	-0.004942186879041799
1113	13	-0.017847234680708368
1113	14	0.06453223478045089
1113	15	0.11709557233373857
1113	16	0.27903212277898604
1113	17	0.16365981909918048
1113	18	0.3064705032637036
1113	19	0.12488586379220834
1113	20	-0.39921728285577984
1113	21	-0.3203086706891156
1113	22	0.19773565478480037
1113	23	-0.1304750432396915
1113	24	0.1411290545653614
1113	25	-0.9716093632058929
1114	1	-0.9798134536559822
1114	2	-0.8194997232651673
1114	3	-0.5277868499248122
1114	4	-1.5089493817623207
1114	5	-0.8151531715159663
1114	6	-0.7075850550664455
1114	7	-0.4987408104005837
1114	8	-0.37460716966380403
1114	9	-0.4188643327230466
1114	10	-0.5149835335762845
1114	11	-0.5590314759872683
1114	12	-0.6401020042306342
1114	13	-0.7281212072834435
1114	14	-0.6286184501510519
1114	15	-1.3543656148808765
1114	16	-0.938319475394529
1114	17	-0.928121002818628
1114	18	-0.7697852044988522
1114	19	-0.9438355973950081
1114	20	-2.175929611622905
1114	21	-7.10957807708181
1114	22	-7.785825989050817
1114	23	-8.061296961047908
1114	24	-6.165948571005412
1114	25	-7.545125344941052
1115	1	-1.1208340501778906
1115	2	-1.1138407582138967
1115	3	-0.9141639552832643
1115	4	-0.523813190352449
1115	5	-0.8281273463744299
1115	6	-1.1103828523337853
1115	7	-1.2541376820609358
1115	8	-0.7743647277571242
1115	9	-2.9224863883124605
1115	10	-1.8687581641725401
1115	11	-1.4433281736546988
1115	12	-1.1842036885173997
1115	13	-1.6392949529005065
1115	14	-1.2869846831602487
1115	15	-2.2320759740835143
1115	16	-1.5766248550435407
1115	17	-3.471136776998783
1115	18	-3.0552773306569634
1115	19	-2.4131466031093893
1115	20	-2.0647823623890886
1115	21	-1.5612692087423383
1115	22	-1.0414116474417838
1115	23	-0.5591748215532846
1115	24	-1.424981188053188
1115	25	-1.2085212538994767
1116	1	-1.5897107926577156
1116	2	-0.7930982116921861
1116	3	-0.6704550911466676
1116	4	-0.2253291644737061
1116	5	-0.13380913120735016
1116	6	-0.27961402688190873
1116	7	-0.6726409836016529
1116	8	-1.592647793187659
1116	9	-0.81080386980382
1116	10	-2.280439682214297
1116	11	-1.2027099518178446
1116	12	-0.8142641834150197
1116	13	-0.8297436289493234
1116	14	-1.1347157576616809
1116	15	-1.2018948228796162
1116	16	-0.6472153972965913
1116	17	-0.5990395983154002
1116	18	-1.2612781977282665
1116	19	-0.702540053386187
1116	20	-1.0150270765890497
1116	21	-0.445521393095035
1116	22	-0.09076914760852617
1116	23	0.04258179820260466
1116	24	-0.1102130962770218
1116	25	-0.15166478754851198
1201	1	0.21176096991301208
1201	2	0.44333373023158984
1201	3	0.29106891155786485
1201	4	0.17077339415409654
1201	5	0.233129198389463
1201	6	0.2029935671538965
1201	7	0.21745509838831367
1201	8	0.34280026915887163
1201	9	0.58874625296761
1201	10	0.5056110633107735
1201	11	0.36200489818749304
1201	12	0.48532316223104743
1201	13	0.438378644279382
1201	14	1.0240979500355536
1201	15	0.8727255820881794
1201	16	0.8484519455025961
1201	17	0.7281197198583342
1201	18	0.6444697115988092
1201	19	0.6234601601293477
1201	20	0.828713644381736
1201	21	0.8452574403984343
1201	22	0.9514479976887955
1201	23	0.6184088506206677
1201	24	0.7010969638631863
1201	25	0.7028255152876852
1202	1	-0.5751575565081879
1202	2	-0.26086308499450794
1202	3	-0.401945952370685
1202	4	-0.5292432822149422
1202	5	-0.4277915740844378
1202	6	-0.41645954376751604
1202	7	-0.3568465104487544
1202	8	-0.3029623093884197
1202	9	-0.20659457182190977
1202	10	0.10052049851556433
1202	11	-0.054466855929625524
1202	12	-0.06785848402071082
1202	13	-0.0915614938110636
1202	14	0.12981148967673306
1202	15	0.09523559256780345
1202	16	0.015508037993761548
1202	17	-0.04854257342056911
1202	18	-0.19201582813926166
1202	19	-0.07788842975255506
1202	20	0.16525508256351173
1202	21	0.0799329486017939
1202	22	-0.03182958360151389
1202	23	0.32322474111082755
1202	24	0.04393196789452958
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2211	9	0.45186888466609076
2211	10	0.3855712977601726
2211	11	0.2667648106873486
2211	12	0.3576550252749797
2211	13	0.20363837548338637
2211	14	0.5293634986447557
2211	15	0.3048810900950798
2211	16	0.39501110970882536
2211	17	0.3628942555616632
2211	18	0.36541256953857015
2211	19	0.3984520914680232
2211	20	0.4390700202179829
2211	21	0.41215959243990596
2211	22	0.44426987750757974
2211	23	0.5162117840273197
2211	24	0.5589849270143077
2211	25	0.6825124113832501
2212	1	-0.16915248745222655
2212	2	-0.2373106376975791
2212	3	-0.11929488964387502
2212	4	-0.5105557533435601
2212	5	-0.10530381377260767
2212	6	-0.414503997538489
2212	7	-0.4408808872602492
2212	8	-0.2457605363733748
2212	9	-0.24891035419074825
2212	10	-0.681517009758096
2212	11	-0.6878200490073212
2212	12	-0.6396635761928025
2212	13	-0.8048097279706496
2212	14	-0.4503819550597896
2212	15	-0.7021516880525382
2212	16	-0.5001635459473022
2212	17	-0.6417142934321802
2212	18	-0.537611728945933
2212	19	-0.6201236482095354
2212	20	-0.5858167029268913
2212	21	-0.47544605004841145
2212	22	-0.39010751285777445
2212	23	-0.052390605413592084
2212	24	-0.1531703702224405
2212	25	-0.9561880163423808
2213	1	-0.1382401313676418
2213	2	-1.3902355754247253
2213	3	-0.28367236779069316
2213	4	0.1529843848432293
2213	5	0.07650907918500849
2213	6	0.041708491938720726
2213	7	0.09609362641658947
2213	8	0.19304847028575978
2213	9	0.07926938880920176
2213	10	-0.5249506219422955
2213	11	-0.05843445772851652
2213	12	0.03107219597198707
2213	13	-0.10018590980978814
2213	14	0.06335241056633834
2213	15	0.03269783899309431
2213	16	-0.03577110788060622
2213	17	0.17243713463871302
2213	18	0.09825802526739835
2213	19	0.16883223468053643
2213	20	0.1703452421426448
2213	21	-0.49787729073536724
2213	22	-0.9538404896609336
2213	23	-0.226346369105471
2213	24	-0.006649765447996003
2213	25	-0.6423752642354685
2214	1	-0.23131267910348186
2214	2	-0.5745217432656524
2214	3	-0.4645034772555654
2214	4	-0.5321135706720312
2214	5	-0.15048898666150734
2214	6	-0.14007449114355808
2214	7	-0.4941985263223003
2214	8	-0.12551960062319978
2214	9	-0.13812879171231884
2214	10	-1.8588834257066722
2214	11	-1.0003623680608769
2214	12	-0.5804699774984385
2214	13	-0.3668901959855262
2214	14	-0.8520843495646631
2214	15	-0.626256861221016
2214	16	-0.715308372123566
2214	17	-0.44421121588139556
2214	18	-0.4785735367372652
2214	19	-1.4510707693781058
2214	20	-2.5362317682041677
2214	21	-2.095328338645352
2214	22	-2.396240881546177
2214	23	-2.795952102697747
2214	24	-1.610940541612969
2214	25	-1.906177925295804
2215	1	-0.2412061318539962
2215	2	-0.40063991130798016
2215	3	-0.8798865449819466
2215	4	-0.1677516321835668
2215	5	-0.14489192337624246
2215	6	-0.14801235586195816
2215	7	-0.23660925658816012
2215	8	-0.27871675870490265
2215	9	-0.20938730497991287
2215	10	-0.29180121201228815
2215	11	-0.6341722859739036
2215	12	-0.5170377250667073
2215	13	-0.35635396658801
2215	14	-0.22828463132075427
2215	15	-0.5926327714869686
2215	16	-0.34721089225237733
2215	17	-0.1817233585895366
2215	18	-0.19824675028311844
2215	19	-2.3461161638715993
2215	20	-2.5307768070052887
2215	21	-3.4313020038320587
2215	22	-2.5020446023064693
2215	23	-3.599357543453877
2215	24	-2.493818952834495
2215	25	-1.9135797827958534
2216	1	-1.7249603994956253
2216	2	-1.2945831116024493
2216	3	-0.8174322833029706
2216	4	-0.9639401516881634
2216	5	-1.807826528074493
2216	6	-1.727719794147717
2216	7	-1.2707790390264009
2216	8	-5.660577103976738
2216	9	-4.338767761086729
2216	10	-2.235095094523601
2216	11	-1.1070249790504292
2216	12	-1.2089376500828664
2216	13	-1.903515088491389
2216	14	-3.4927129322256505
2216	15	-2.182838305273954
2216	16	-3.039365021016039
2216	17	-2.806690523690122
2216	18	-2.753693696755356
2216	19	-1.6658061152792314
2216	20	-0.994049617882176
2216	21	-0.8588949104074999
2216	22	-0.6305762005365807
2216	23	-1.001571557224807
2216	24	-0.6101999819890267
2216	25	-0.2316497896364922
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	10.0
7	110.0
8	561.0
9	1212.0
10	2221.0
11	3716.0
12	6224.0
13	9168.0
14	11989.0
15	14717.0
16	16408.0
17	17624.0
18	18585.0
19	19177.0
20	19784.0
21	21392.0
22	22452.0
23	23710.0
24	24828.0
25	26732.0
26	28854.0
27	32413.0
28	36641.0
29	43221.0
30	50299.0
31	61937.0
32	80929.0
33	111718.0
34	156335.0
35	237103.0
36	395153.0
37	1137816.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.935207729681494	20.56952948492977	12.751292338891204	23.74397044649753
2	15.828139108119021	20.66899620181184	39.67611889669832	23.826745793370815
3	19.18500537530248	25.35326943751163	28.71760098161001	26.74412420557588
4	12.874344308629293	16.783989581877083	37.243459801484306	33.09820630800931
5	13.394454077004085	38.43502732996084	34.54694720462444	13.623571388410639
6	32.34603060776452	37.82518660769767	16.639496565628328	13.18928621890948
7	27.092515028735765	32.86579798523029	22.058401279778316	17.983285706255632
8	24.84058967293775	37.30598541199437	20.253248689225348	17.60017622584253
9	24.533341739537313	17.464914226335523	21.446378546557206	36.55536548756996
10	14.930970297345572	30.764106453596412	32.960994638429135	21.343928610628875
11	33.14413346205761	25.65516073190536	22.227876907791742	18.972828898245282
12	21.137069533242133	27.587661896214755	30.693342306573378	20.581926263969734
13	27.02302786496234	23.7727992270367	26.09149922673286	23.1126736812681
14	20.6874746489593	23.375292628866838	27.491731952717558	28.445500769456306
15	23.175985388513546	30.93505233769731	23.405647632716633	22.48331464107251
16	22.03875132263879	31.076485552188878	25.375940849086632	21.508822276085702
17	20.635559054747286	30.28440301117855	27.77664471822708	21.303393215847084
18	20.842237992240744	29.820640831603672	28.689984941046	20.64713623510958
19	21.348595821931728	28.93580981642857	28.968015996748996	20.747578364890707
20	22.36760600236002	28.50585503930641	28.328340988009636	20.798197970323933
21	22.460902831492106	28.60432278135803	27.899709039907716	21.035065347242153
22	21.590559237757574	28.3544801278918	28.63008148313134	21.42487915121928
23	21.70973287585582	28.592192399016476	28.360831015728543	21.337243709399157
24	21.680658864457666	28.269341420386894	28.46649183540542	21.58350787975002
25	21.991318194674808	28.145783979659466	27.816342853270793	22.046554972394937
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2949.0
1	2949.0
2	3235.0
3	3521.0
4	3521.0
5	3521.0
6	4693.0
7	5865.0
8	5865.0
9	5865.0
10	8564.0
11	11263.0
12	11263.0
13	11263.0
14	17913.5
15	24564.0
16	24564.0
17	24564.0
18	37575.0
19	50586.0
20	50586.0
21	50586.0
22	71436.0
23	92286.0
24	92286.0
25	92286.0
26	119041.5
27	145797.0
28	145797.0
29	145797.0
30	175342.5
31	204888.0
32	204888.0
33	204888.0
34	229737.0
35	254586.0
36	254586.0
37	254586.0
38	271922.0
39	289258.0
40	289258.0
41	289258.0
42	291958.5
43	294659.0
44	294659.0
45	294659.0
46	302046.5
47	309434.0
48	309434.0
49	309434.0
50	295629.0
51	281824.0
52	281824.0
53	281824.0
54	257813.0
55	233802.0
56	233802.0
57	233802.0
58	205890.0
59	177978.0
60	177978.0
61	177978.0
62	149909.0
63	121840.0
64	121840.0
65	121840.0
66	96085.0
67	70330.0
68	70330.0
69	70330.0
70	52446.5
71	34563.0
72	34563.0
73	34563.0
74	24748.0
75	14933.0
76	14933.0
77	14933.0
78	10134.5
79	5336.0
80	5336.0
81	5336.0
82	3630.5
83	1925.0
84	1925.0
85	1925.0
86	1259.5
87	594.0
88	594.0
89	594.0
90	398.0
91	202.0
92	202.0
93	202.0
94	123.0
95	44.0
96	44.0
97	44.0
98	28.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030269194543189618
2	0.017812110716130404
3	0.02392671588733935
4	0.02673715553124905
5	0.02901589037766232
6	0.009266855042080637
7	0.0031902287849785798
8	0.002088840275878832
9	0.0024306505028408227
10	0.0016330933065961779
11	8.355361103515328E-4
12	9.874517667790843E-4
13	0.0021268191899857197
14	4.557469692826543E-4
15	0.0016330933065961779
16	0.0017470300489168412
17	0.0019369246194512804
18	0.002088840275878832
19	1.898945705344393E-4
20	3.418102269619907E-4
21	0.07956582505393006
22	0.0784644365448303
23	0.07941390939750251
24	9.494728526721963E-4
25	0.2342159632971774
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2633040.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.6192742321332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.18810322719591	71.9028015231711
2	8.008900166226697	12.913434375976143
3	1.479085645939586	3.5772843390844606
4	0.5128185673013487	1.6537224283438832
5	0.23382845101692146	0.9425540007904057
6	0.1301144606365002	0.6293840030172067
7	0.08591659221672429	0.4848573316307319
8	0.05571849185151408	0.3593587499502474
9	0.044832818331085725	0.3252950348069888
>10	0.23877733633359113	3.3493399171836433
>50	0.014078882028677596	0.7689760293484351
>100	0.006029528581900137	0.9162445144214791
>500	8.979161698063346E-4	0.47704581603805707
>1k	8.033986781794294E-4	1.2343527151935458
>5k	9.451749155052109E-5	0.46534922104370546
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	6308	0.23957099018624858	No Hit
CCCATGTACTCTGCGTTGATACCAC	5906	0.22430346671527968	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3549	0.134787166165345	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3174	0.12054507337526206	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	3.7978914106887855E-5	0.0	0.0	0.0
4	0.0	3.7978914106887855E-5	0.0	0.0	0.0
5	0.0	3.7978914106887855E-5	0.0	0.0	0.0
6	0.0	3.7978914106887855E-5	0.0	0.0	0.0
7	0.0	3.7978914106887855E-5	0.0	0.0	0.0
8	0.0	3.7978914106887855E-5	0.0	0.0	0.0
9	0.0	3.7978914106887855E-5	0.0	0.0	0.0
10	0.0	3.7978914106887855E-5	0.0	7.595782821377571E-5	0.0
11	0.0	3.7978914106887855E-5	0.0	7.595782821377571E-5	0.0
12	0.0	3.7978914106887855E-5	0.0	7.595782821377571E-5	0.0
13	0.0	3.7978914106887855E-5	0.0	7.595782821377571E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATGA	65	8.05607E-4	11.687213	13
CTCCGGT	60	0.005896594	11.078504	7
TAGACAG	435	0.0	10.480977	5
GTGTAGG	370	0.0	9.758806	1
GTACTAG	195	1.6370905E-10	9.745636	1
GTAAGAC	265	0.0	9.678151	3
TAATACT	445	0.0	9.605291	4
GTTCTAG	230	3.6379788E-12	9.501996	1
AGCGTTA	80	0.0045348993	9.495499	10
GTCTTAG	285	0.0	9.335294	1
GTCTAGA	265	0.0	9.322712	1
CTAGGAC	370	0.0	9.242199	3
GGACAGT	350	0.0	8.95375	6
TAAGACA	410	0.0	8.803549	4
GTGCTAG	295	0.0	8.696741	1
GTCCTAC	230	4.0199666E-10	8.675735	1
GTATAGG	330	0.0	8.638178	1
TGTACTG	465	0.0	8.579186	5
TATCGGT	100	0.0029172804	8.546273	13
CTATACT	425	0.0	8.492836	4
>>END_MODULE