FastQCFastQC Report
Wed 25 May 2016
SRR1294726_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294726_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2633040
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT107730.40914684167350285No Hit
GGTATCAACGCAGAGTACTTTTTTT86440.3282897335399386No Hit
GTACATGGGAAGCAGTGGTATCAAC71370.27105550998085864No Hit
TATCAACGCAGAGTACTTTTTTTTT70880.26919454318962116No Hit
CCCATGTACTCTGCGTTGATACCAC64500.2449639959894267No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46150.17527268860328746No Hit
GAGTACATGGGAAGCAGTGGTATCA37650.14299061161243276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG801.9650233E-613.0835685
TGAACCG802.8212142E-511.8941535
AGGACCG700.001472071910.8746545
GAAGCGT700.001497508510.8523456
CGGTCAC700.001499407810.85069710
GGAACGA1502.1609594E-910.759047515
TATTCCG803.71678E-410.7047385
GAACGAA1106.1005157E-610.35630316
ACACCGA1201.5334062E-610.2871196
ACCGCTC1054.1305888E-59.94722758
TACACGG1151.0075215E-59.9290325
AGAGCCG1151.0075215E-59.9290325
CTAGTAC1659.962605E-99.8036663
ATTAGAC2151.0913936E-119.73660853
GTACGGA800.0044393819.5200341
CTAGACA2950.09.3540464
TTATACT4050.09.1629034
GTCTTAC2600.09.1538781
TAGGACT2600.09.1493484
GTATTAG4000.09.0440321
Produced by FastQC (version 0.11.5)