FastQCFastQC Report
Wed 25 May 2016
SRR1294725_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294725_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3630598
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1469824.048423978639332No Hit
CCCATGTACTCTGCGTTGATACCAC1452724.001324299743459No Hit
GAGTACATGGGAAGCAGTGGTATCA702161.9340064639489143No Hit
CATGTACTCTGCGTTGATACCACTG591641.62959380245348No Hit
GCGTTGATACCACTGCTTCCCATGT484941.335702823611978No Hit
ACGCAGAGTACATGGGAAGCAGTGG383971.0575943687513738No Hit
GTATCAACGCAGAGTACATGGGAAG378951.04376744547317No Hit
GCTTCCCATGTACTCTGCGTTGATA365701.0072720802468353No Hit
ACTCTGCGTTGATACCACTGCTTCC327600.9023306904262053No Hit
TATCAACGCAGAGTACATGGGAAGC292080.8044955679477596No Hit
GGTATCAACGCAGAGTACATGGGAA243720.6712943707896055No Hit
CAGTGGTATCAACGCAGAGTACATG211060.5813367384656742No Hit
GTGGTATCAACGCAGAGTACATGGG190060.5234950275409175No Hit
GCAGTGGTATCAACGCAGAGTACAT170390.46931662497472865No Hit
ATACCACTGCTTCCCATGTACTCTG167240.4606403683360152No Hit
GTACATGGTAAGCAGTGGTATCAAC154850.42651375889040866No Hit
GTACTCTGCGTTGATACCACTGCTT153660.4232360619380058No Hit
CATGGGAAGCAGTGGTATCAACGCA145770.4015041048334186No Hit
CTGCTTCCCATGTACTCTGCGTTGA145550.4008981440523022No Hit
ACATGGGAAGCAGTGGTATCAACGC139440.3840689605403848No Hit
GGGAAGCAGTGGTATCAACGCAGAG133010.3663583795286617No Hit
TACCACTGCTTCCCATGTACTCTGC127700.35173268976625893No Hit
GATACCACTGCTTCCCATGTACTCT121750.3353442050042445No Hit
AAGCAGTGGTATCAACGCAGAGTAC119370.3287888110994387No Hit
GTTGATACCACTGCTTCCCATGTAC119170.32823793766206005No Hit
CTTCCCATGTACTCTGCGTTGATAC119110.3280726756308465No Hit
GCAGAGTACATGGGAAGCAGTGGTA118380.32606198758441446No Hit
CCACTGCTTCCCATGTACTCTGCGT114810.31622889672720583No Hit
ACCATGTACTCTGCGTTGATACCAC113750.31330926750909904No Hit
CCATGTACTCTGCGTTGATACCACT112980.31118840477519133No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA106170.29243116423244875No Hit
GTACATGGGTAAGCAGTGGTATCAA99840.2749960199394149No Hit
ATGGGAAGCAGTGGTATCAACGCAG95750.26373065814502183No Hit
TTCCCATGTACTCTGCGTTGATACC93480.25747824463077434No Hit
TGATACCACTGCTTCCCATGTACTC83450.22985194174623574No Hit
GGTAAGCAGTGGTATCAACGCAGAG74140.20420878323626027No Hit
CCCCATGTACTCTGCGTTGATACCA67630.18627785284958565No Hit
ATCAACGCAGAGTACATGGGAAGCA59680.1643806337137849No Hit
GCTTACCATGTACTCTGCGTTGATA56570.1558145517625471No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA55600.15314281559126072No Hit
GAGTACATGGTAAGCAGTGGTATCA53890.1484328477016734No Hit
AAAAAGTACTCTGCGTTGATACCAC42470.11697797442735329No Hit
ACTCTGCGTTGATACCACTGCTTAC41980.11562833450577564No Hit
GGAAGCAGTGGTATCAACGCAGAGT41770.11504991739652806No Hit
TCCATGTACTCTGCGTTGATACCAC40180.11067047356936792No Hit
GCGTTGATACCACTGCTTACCATGT39490.10876996021041163No Hit
GAACAAAAAAAAAAAAAAAAAAAAA38850.10700716521079999No Hit
CTGCGTTGATACCACTGCTTCCCAT37170.10237982833681944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAAAA3400.012.8526982
GGACCGT701.09608685E-412.2090096
TCGTGCA550.00308038912.0853539
GCGCAAA658.058039E-411.68709511
ACGCAAA4050.011.0270671
GATACGA1158.763527E-710.74514618
GAACAAA16200.010.6164851
AGGACCG1002.4064122E-510.4477875
GTACTAG1106.035716E-610.3658691
ATACGAC1201.4812704E-610.315207519
GTACAAA8250.010.2506921
CGACCAT750.002658540810.1289566
TATAACG856.5986096E-410.0586342
ACACCGT856.623726E-410.0544786
CTGTCGC1451.6299964E-79.823079
AGTACTC11850.09.61821655
GCCTACG800.0045098829.5020471
GTACGAC800.0045201469.4994293
TACGACC1106.8511166E-59.4987744
GGACCGC900.00111868019.4958966
Produced by FastQC (version 0.11.5)