##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294725_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3630598
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.935315339236126	33.0	33.0	33.0	27.0	33.0
2	30.869766082612287	33.0	33.0	33.0	27.0	33.0
3	31.004754037764577	33.0	33.0	33.0	27.0	33.0
4	34.28632087606505	37.0	37.0	37.0	27.0	37.0
5	34.71900827356816	37.0	37.0	37.0	27.0	37.0
6	34.802151326034995	37.0	37.0	37.0	27.0	37.0
7	34.88123058515429	37.0	37.0	37.0	33.0	37.0
8	34.81125450958768	37.0	37.0	37.0	27.0	37.0
9	34.47237479886233	37.0	37.0	37.0	27.0	37.0
10	34.60129570941206	37.0	37.0	37.0	27.0	37.0
11	34.83617988000875	37.0	37.0	37.0	33.0	37.0
12	34.789256756049554	37.0	37.0	37.0	27.0	37.0
13	34.621553253761505	37.0	37.0	37.0	27.0	37.0
14	35.79488585626941	40.0	37.0	40.0	27.0	40.0
15	36.01032281734304	40.0	37.0	40.0	27.0	40.0
16	35.96318485274327	37.0	37.0	40.0	27.0	40.0
17	35.978277683180565	37.0	37.0	40.0	27.0	40.0
18	35.94583867451037	37.0	37.0	40.0	27.0	40.0
19	35.901435245653744	37.0	37.0	40.0	27.0	40.0
20	35.84119668440295	37.0	37.0	40.0	27.0	40.0
21	35.75677395294109	37.0	37.0	40.0	27.0	40.0
22	35.696183934437244	37.0	37.0	40.0	27.0	40.0
23	35.32562431863842	37.0	33.0	40.0	27.0	40.0
24	35.67829817567244	37.0	37.0	40.0	27.0	40.0
25	34.49778631509189	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6092908740349046
1101	2	0.7220867693714403
1101	3	0.6052641934835137
1101	4	0.6767761904883898
1101	5	0.6301344471296346
1101	6	0.48081425549844425
1101	7	0.5403046715435877
1101	8	0.7267534143507604
1101	9	0.8316808196839887
1101	10	0.8826448046274393
1101	11	0.5850461079807303
1101	12	0.6547635600923272
1101	13	0.7099000491809733
1101	14	1.3473109658934987
1101	15	1.1672220417982118
1101	16	1.0982813887439349
1101	17	1.1521209759901154
1101	18	1.072855706843427
1101	19	0.9784090442371536
1101	20	1.0969610693649514
1101	21	1.178041956246254
1101	22	1.2759314180342258
1101	23	1.627538742383308
1101	24	1.3829565850141634
1101	25	1.309561132092334
1102	1	0.3920512386470669
1102	2	0.5515186573253779
1102	3	0.4665696750103514
1102	4	0.39980052643573316
1102	5	0.4810570888428245
1102	6	0.31658882853965054
1102	7	0.35414484491089837
1102	8	0.4983576835388632
1102	9	0.7550179723682717
1102	10	0.8217516812936694
1102	11	0.5863192548300162
1102	12	0.5094400228084268
1102	13	0.63797481205183
1102	14	1.3154927440615367
1102	15	1.1320818851028989
1102	16	0.8223158221717526
1102	17	1.0182697040830817
1102	18	0.9927813487776262
1102	19	0.9336326926475635
1102	20	1.015844862425972
1102	21	1.0075057971890473
1102	22	1.0296987963778435
1102	23	1.2598439734605336
1102	24	1.1304258646177345
1102	25	1.0854689749685704
1103	1	0.48449115138610566
1103	2	0.6386093136670752
1103	3	0.4841015903773389
1103	4	0.3633180363014574
1103	5	0.4751000436643622
1103	6	0.38852177961738477
1103	7	0.3947720718088661
1103	8	0.4884340865416945
1103	9	0.7133038239274327
1103	10	0.767511879451753
1103	11	0.5181453373184581
1103	12	0.5443101742543774
1103	13	0.5161160417967849
1103	14	1.2224908634190967
1103	15	0.8969151030673856
1103	16	0.6657615097981093
1103	17	0.6855369641521918
1103	18	0.8036575300391888
1103	19	0.9185776208524743
1103	20	0.983722440694585
1103	21	1.0341064720802464
1103	22	0.9378414444511165
1103	23	1.4750225894660858
1103	24	1.1035808558554834
1103	25	0.9865869911417491
1104	1	0.5256735658908092
1104	2	0.6307474946369496
1104	3	0.5226547269712789
1104	4	0.46981296855271637
1104	5	0.4169004605646336
1104	6	0.33718033052284824
1104	7	0.37810261350603014
1104	8	0.5725931802025102
1104	9	0.8304784434102572
1104	10	0.7295897786843568
1104	11	0.5000883304344725
1104	12	0.4791969721581495
1104	13	0.6167694346192434
1104	14	1.093486433030563
1104	15	0.949414076008253
1104	16	0.8217233655943801
1104	17	0.8206967729891161
1104	18	0.815265009781136
1104	19	0.916347365440906
1104	20	0.9684781838312801
1104	21	1.015466742388
1104	22	1.0964478023008866
1104	23	1.5404370144293509
1104	24	1.2121775674015325
1104	25	1.0440533051994478
1105	1	0.3224200815356717
1105	2	0.433160316936565
1105	3	0.4209011401344043
1105	4	0.40286534409943187
1105	5	0.3281114489215682
1105	6	0.2705958035271081
1105	7	0.25597709620786446
1105	8	0.3400288890198837
1105	9	-1.49820092996395
1105	10	0.01513200416739835
1105	11	0.1993989916630028
1105	12	0.32096207641938435
1105	13	0.27712359879296855
1105	14	-1.571314252756622
1105	15	-0.1419600725391419
1105	16	-0.042648950688089826
1105	17	0.19542620560088864
1105	18	0.24683270981153527
1105	19	0.2713970251330622
1105	20	0.48170501393391163
1105	21	0.6104019426535885
1105	22	0.6643146768944419
1105	23	0.9665557455944906
1105	24	0.788977340125733
1105	25	0.47559715656971235
1106	1	0.06830748492761529
1106	2	0.051195502410106286
1106	3	0.035809608073805066
1106	4	-1.0675147963343647
1106	5	-0.2130559492166526
1106	6	-0.13741460524972382
1106	7	0.023312081538470864
1106	8	0.08362317482794168
1106	9	0.3368481309751985
1106	10	0.4003581414798276
1106	11	0.31782730864095043
1106	12	0.06375799583801012
1106	13	0.20825040428051977
1106	14	0.5437837423787357
1106	15	0.35559926934100616
1106	16	0.22969466474437894
1106	17	0.13419451795866877
1106	18	0.2550433365601421
1106	19	0.21370973583913866
1106	20	0.35413528435181973
1106	21	0.5190677445973293
1106	22	0.56838696859257
1106	23	0.6880029654104476
1106	24	0.31529493420333665
1106	25	0.2845561898234408
1107	1	0.37682090105409216
1107	2	0.3599421268013927
1107	3	0.2783692164102334
1107	4	0.48073411077820793
1107	5	0.4831060498439612
1107	6	0.3268025114869175
1107	7	0.2806414545417866
1107	8	0.33888075213275215
1107	9	0.5439299254583219
1107	10	0.4615065879744975
1107	11	0.24120682797376958
1107	12	0.27024773027308413
1107	13	0.2969363337340738
1107	14	0.8588599106532584
1107	15	0.6406908862815115
1107	16	0.6162434820275564
1107	17	0.6257591326544443
1107	18	0.6310500981787825
1107	19	0.5620655287573015
1107	20	0.7366272725545073
1107	21	0.6700054782669369
1107	22	0.642141648103113
1107	23	0.8508942805301984
1107	24	0.8494155177971479
1107	25	0.7470832176863453
1108	1	0.4572763767238932
1108	2	0.4104005376890889
1108	3	0.4368525248130659
1108	4	0.6761334487018118
1108	5	0.5117833902602271
1108	6	0.41965317616990205
1108	7	0.40784824231882766
1108	8	0.48787487355483705
1108	9	0.26776092160245923
1108	10	0.5067378646866985
1108	11	0.36623347869856815
1108	12	0.29769656760516483
1108	13	0.35683076831115557
1108	14	-1.0479792133586443
1108	15	0.05591114975553779
1108	16	0.2928606122716175
1108	17	0.3458322614696172
1108	18	0.5784702398598753
1108	19	0.5455957768832391
1108	20	0.6724716325427167
1108	21	0.7241034930287924
1108	22	0.8007926621901333
1108	23	1.0132347459849456
1108	24	0.9367271054162387
1108	25	0.8099949914174829
1109	1	0.42452197868834673
1109	2	0.30805531801008
1109	3	0.417097058949242
1109	4	0.5306541311185811
1109	5	0.5762645002173272
1109	6	0.3531928881338189
1109	7	0.28396441502361824
1109	8	0.3593753694887596
1109	9	0.4878842112147268
1109	10	0.46692504870228646
1109	11	0.3254114992575481
1109	12	0.3537782951789481
1109	13	0.25853342122898226
1109	14	0.7430870226708421
1109	15	0.4774577624318752
1109	16	0.47922618350131074
1109	17	0.5046879468882821
1109	18	0.44915652768852965
1109	19	0.4184923669905629
1109	20	0.5971762414414812
1109	21	0.5300014986294173
1109	22	0.6706238401464191
1109	23	1.0314161657729244
1109	24	0.7993965592461834
1109	25	0.6230410482680142
1110	1	0.2553985373257497
1110	2	0.14902793924061442
1110	3	0.2647435039462174
1110	4	0.3849729970904363
1110	5	0.3336324624621909
1110	6	0.21736247131863706
1110	7	0.1774759028844599
1110	8	0.06879127857641976
1110	9	0.2842490629840313
1110	10	0.3373519697036542
1110	11	0.12842231936098614
1110	12	0.1427187151861915
1110	13	0.04310403102820004
1110	14	0.4907081601630239
1110	15	0.313368510534886
1110	16	0.3622575301668931
1110	17	0.2765488643998424
1110	18	0.4003134728665785
1110	19	0.2936436322942342
1110	20	0.4854618440764398
1110	21	0.467965504143244
1110	22	0.4888705511012148
1110	23	0.7329874354567423
1110	24	0.6933915521391825
1110	25	0.5739655308454914
1111	1	0.1127282064591455
1111	2	0.031634488852979814
1111	3	0.15246010593030235
1111	4	0.35623400399187233
1111	5	0.2323058100537878
1111	6	0.12112921482323458
1111	7	0.041647122661792935
1111	8	0.24164115142797016
1111	9	0.2502936979290098
1111	10	0.056276770156927114
1111	11	0.06562633747337543
1111	12	0.09968262450732368
1111	13	-0.0034908791723609056
1111	14	-1.9172429316125985
1111	15	-0.650357865779597
1111	16	-0.23849044150873056
1111	17	-0.011387111156096807
1111	18	0.06154228915890769
1111	19	0.05386708628413572
1111	20	0.16452903708719901
1111	21	0.2986199789450765
1111	22	0.09856160472940445
1111	23	-1.9304559716558671
1111	24	-0.5232195612323878
1111	25	-0.09396669521176904
1112	1	-0.11359206087987772
1112	2	-0.29038306520918056
1112	3	0.018446022071113788
1112	4	0.1645702614689739
1112	5	-0.7400604563665922
1112	6	-0.8857693824419997
1112	7	-1.0140263569312964
1112	8	-0.7571578140165371
1112	9	-0.6594926063015336
1112	10	-0.9146498482909351
1112	11	-0.811242568233169
1112	12	-0.7900378930595551
1112	13	-0.9903089869162116
1112	14	-0.6314095210687256
1112	15	-0.9303520240631684
1112	16	-0.9458970841119978
1112	17	-0.9909699344070475
1112	18	-1.1200784980205967
1112	19	-0.875717971144077
1112	20	-1.1109446084921188
1112	21	-1.9510420196053389
1112	22	-1.884573149672086
1112	23	-1.5449501637806549
1112	24	-1.7916295925568662
1112	25	-2.05044024025311
1113	1	0.011573637792608338
1113	2	-0.1234354071979773
1113	3	-0.16014462062970836
1113	4	0.15814197649555695
1113	5	0.010125167046197703
1113	6	-0.17471025620348968
1113	7	-0.11264771413054575
1113	8	-0.0144312009084544
1113	9	0.21623535140871297
1113	10	-0.054078538280990074
1113	11	-0.2183947154293051
1113	12	-0.1438190947607083
1113	13	-0.26058908399640046
1113	14	-0.08161447230865804
1113	15	-0.20802071780272513
1113	16	-0.024805494640574466
1113	17	-0.05931148966650568
1113	18	-0.1197661963698664
1113	19	-0.2107598302323339
1113	20	-0.8033775569048913
1113	21	-0.7612502461299044
1113	22	-0.14975386734299434
1113	23	-0.4396308605596033
1113	24	-0.07761342483222222
1113	25	-1.2006311916685846
1114	1	-0.9598489640626084
1114	2	-0.7261770350617844
1114	3	-0.6087987859403974
1114	4	-1.7845325112959713
1114	5	-1.0158072929344613
1114	6	-0.7447304023569714
1114	7	-0.6495584919052959
1114	8	-0.5362949966293655
1114	9	-0.551420590045467
1114	10	-0.6801886768216292
1114	11	-0.6646971049412755
1114	12	-0.8191486288296446
1114	13	-0.7824578769617148
1114	14	-0.6573365580713713
1114	15	-1.407167942234075
1114	16	-0.906824490813662
1114	17	-1.0484734716026693
1114	18	-1.0038969889403688
1114	19	-1.0403250412147358
1114	20	-2.2523077990303193
1114	21	-7.113121606673801
1114	22	-8.028049832687913
1114	23	-7.93603009471375
1114	24	-6.158699047422292
1114	25	-7.3554882298587785
1115	1	-1.1920517789733545
1115	2	-1.1289945304994262
1115	3	-0.8696467865072037
1115	4	-0.7436574201285993
1115	5	-0.797542134369003
1115	6	-0.971342683074468
1115	7	-1.1647811196874684
1115	8	-0.8475059410008896
1115	9	-2.9374361660004844
1115	10	-1.7679803762847328
1115	11	-1.2507534277867975
1115	12	-1.1310897027443545
1115	13	-1.4910868064329463
1115	14	-1.180382923319499
1115	15	-2.130848256118931
1115	16	-1.5029386970497853
1115	17	-3.2295557242184927
1115	18	-3.0917079367480085
1115	19	-2.300725616597319
1115	20	-2.026824967382936
1115	21	-1.4232909206466857
1115	22	-1.0245630239615693
1115	23	-0.39757237364172227
1115	24	-1.424999377244042
1115	25	-1.2392653267310152
1116	1	-1.7083247194595579
1116	2	-0.9003999683367745
1116	3	-0.662346870615167
1116	4	-0.3363388959346878
1116	5	-0.4471160549704578
1116	6	-0.4578425356124498
1116	7	-0.9974075196013317
1116	8	-1.7870142917201761
1116	9	-1.0436296096364472
1116	10	-2.660362636838407
1116	11	-1.2640852422873863
1116	12	-0.9654355565650263
1116	13	-1.0083789903219866
1116	14	-1.3112775418142064
1116	15	-1.3452583768505306
1116	16	-0.9457310814559534
1116	17	-0.7252028249904399
1116	18	-1.359936621464854
1116	19	-1.0324167640390485
1116	20	-1.2946281188568562
1116	21	-0.572688823097053
1116	22	-0.26409407914981387
1116	23	-0.2342586212874025
1116	24	-0.18474131091578982
1116	25	-0.24179558765295894
1201	1	0.5577497826304345
1201	2	0.4963715199487311
1201	3	0.524002755293715
1201	4	0.6490553047748548
1201	5	0.6515175464907799
1201	6	0.4125932893199291
1201	7	0.47882100967613184
1201	8	0.5583701615447794
1201	9	0.84954147118831
1201	10	0.7772361548297226
1201	11	0.6686178733822885
1201	12	0.6348599402912072
1201	13	0.7217485167910169
1201	14	1.3136653785553207
1201	15	1.0891307832885673
1201	16	1.0666595890979806
1201	17	0.9988918042554076
1201	18	0.9016966463000813
1201	19	0.9270742612867409
1201	20	1.0983958721509097
1201	21	1.050763440768904
1201	22	1.1808943292944534
1201	23	1.0666520183557182
1201	24	1.039992171117305
1201	25	1.037938509083844
1202	1	-0.026198007205707086
1202	2	0.26421453261099614
1202	3	0.20525949146975364
1202	4	0.2092350663618845
1202	5	0.28402803035698554
1202	6	0.16242107547215312
1202	7	0.2829625463002543
1202	8	0.357698839678136
1202	9	0.40155445002402956
1202	10	0.6010648455829255
1202	11	0.37079773619655754
1202	12	0.31260923317731937
1202	13	0.48104135188943076
1202	14	0.6192185921308351
1202	15	0.7413270116207045
1202	16	0.6409331292642548
1202	17	0.45359254753721956
1202	18	0.42507896386147337
1202	19	0.5129584583990052
1202	20	0.652812024581408
1202	21	0.7284148627565372
1202	22	0.7070790907552862
1202	23	0.96966705959575
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2211	6	0.3461573326915044
2211	7	0.2842498437754273
2211	8	0.4217987677385935
2211	9	0.5481728673894324
2211	10	0.4524533410275353
2211	11	0.2362573954760876
2211	12	0.29809307649273364
2211	13	0.1992260714810925
2211	14	0.4411051019012433
2211	15	0.34989642016367384
2211	16	0.16705460917007997
2211	17	0.35325573550906597
2211	18	0.33778382545793306
2211	19	0.43260724625946523
2211	20	0.50344100902457
2211	21	0.4392205472191151
2211	22	0.5654801207396929
2211	23	0.6567319011582171
2211	24	0.641886261219156
2211	25	0.5501848073097548
2212	1	-0.35682979943263504
2212	2	-0.33423822111897294
2212	3	-0.22686428823185167
2212	4	-0.7104554843841129
2212	5	-0.18732949404898136
2212	6	-0.5660740433342539
2212	7	-0.5328414691722116
2212	8	-0.2750143671739522
2212	9	-0.16724275019700485
2212	10	-0.49084895069631784
2212	11	-0.4855293958719642
2212	12	-0.6231063682225724
2212	13	-0.6033204090241853
2212	14	-0.48724668692608475
2212	15	-0.6920861399725879
2212	16	-0.4469283553553822
2212	17	-0.5353556933911321
2212	18	-0.40638171029582537
2212	19	-0.46659578731843965
2212	20	-0.4324707162548407
2212	21	-0.3520787824819962
2212	22	-0.4113589272959217
2212	23	0.04850294097353469
2212	24	-0.3133961824874376
2212	25	-0.8839080207551007
2213	1	-0.18353935435152735
2213	2	-1.3013948122125818
2213	3	-0.3264552685515838
2213	4	-0.07441464845663859
2213	5	-0.03697859431331807
2213	6	-0.07191404117912015
2213	7	-0.051029165549365985
2213	8	0.08848141842313595
2213	9	0.046021476905636405
2213	10	-0.3944952953789951
2213	11	-0.10795338097427987
2213	12	0.043789666735385424
2213	13	-0.39883571416590513
2213	14	-7.12544476364485E-4
2213	15	-0.12135372650836729
2213	16	-0.14117512239355534
2213	17	0.07985466857860501
2213	18	0.012349367276222267
2213	19	0.03699132154102358
2213	20	0.14390673555394784
2213	21	-0.49844353273407194
2213	22	-0.8717172331817835
2213	23	-0.10459131744485717
2213	24	0.09592566676241177
2213	25	-0.4911147053727163
2214	1	-0.22093409529231778
2214	2	-0.4526111193776714
2214	3	-0.4386133872530067
2214	4	-0.6103124068971368
2214	5	-0.3274821720539336
2214	6	-0.27615328481891055
2214	7	-0.5044827298169707
2214	8	-0.16310135233222667
2214	9	-0.10557802782128789
2214	10	-2.1178465003315523
2214	11	-1.0091576474427413
2214	12	-0.5010588531008224
2214	13	-0.3915675888720287
2214	14	-0.9010420358844442
2214	15	-0.7198736857192429
2214	16	-0.8905895017242074
2214	17	-0.5631730081857853
2214	18	-0.556118161192714
2214	19	-1.5349920356741649
2214	20	-2.5247677890271376
2214	21	-2.0284427165361123
2214	22	-2.1164667377638366
2214	23	-2.7410168656764995
2214	24	-1.6908379421358646
2214	25	-2.085360750297717
2215	1	-0.4320421028572916
2215	2	-0.46219502691793934
2215	3	-1.0530433293819073
2215	4	-0.5308809974919058
2215	5	-0.4509972292119784
2215	6	-0.4439063235236276
2215	7	-0.43930116378736983
2215	8	-0.2992481913094025
2215	9	-0.3538873150711197
2215	10	-0.43529372048644177
2215	11	-0.7598433871601671
2215	12	-0.5107803696080708
2215	13	-0.47563680731679625
2215	14	-0.3385954153509587
2215	15	-0.7904731648877785
2215	16	-0.5749248281624375
2215	17	-0.448773388895205
2215	18	-0.49682646837350575
2215	19	-2.6646065353951798
2215	20	-2.7929297366602768
2215	21	-3.485710914725722
2215	22	-2.6077866834986025
2215	23	-3.7315243774943347
2215	24	-2.724720957992716
2215	25	-2.0792601733551805
2216	1	-1.5569460957209067
2216	2	-1.1732784092423856
2216	3	-0.8913879591658542
2216	4	-0.837879712236365
2216	5	-1.9192047041178597
2216	6	-1.590168382359046
2216	7	-1.2825160730214549
2216	8	-5.683497130290956
2216	9	-4.49472042355486
2216	10	-2.397668048383224
2216	11	-1.2153222269582855
2216	12	-1.2750529571210762
2216	13	-2.0061549420888554
2216	14	-3.8109299676833217
2216	15	-2.327127767259782
2216	16	-3.2465194380356976
2216	17	-3.0274431037873413
2216	18	-2.908822148846845
2216	19	-1.9362463246796011
2216	20	-1.1531087797835013
2216	21	-0.8720127757559979
2216	22	-0.8219683032401477
2216	23	-1.2184975348381641
2216	24	-0.7036327825460518
2216	25	-0.5248004303800116
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	27.0
8	221.0
9	623.0
10	1242.0
11	2626.0
12	4592.0
13	7027.0
14	10247.0
15	14640.0
16	18791.0
17	21804.0
18	22541.0
19	23414.0
20	24152.0
21	25809.0
22	27126.0
23	28693.0
24	30444.0
25	32620.0
26	34837.0
27	38230.0
28	43029.0
29	50581.0
30	60837.0
31	74165.0
32	100512.0
33	142127.0
34	205681.0
35	324245.0
36	559312.0
37	1700400.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.43936930120915	18.89536789936239	10.934129182603215	24.731133616825247
2	12.59531165419903	23.04672230387059	35.777072862331664	28.58089317959871
3	21.750145322008777	28.443277582943743	26.744566609823494	23.06201048522399
4	13.572323748425863	19.613451985512025	32.156123433750196	34.658100832311916
5	11.555868267300221	40.43063492728166	34.73700072949749	13.276496075920633
6	33.11728681566255	32.13865944467138	18.16490289888885	16.579150840777203
7	24.46585934328174	33.82734745075054	22.159545066680476	19.547248139287245
8	27.22715485245491	35.506206300086625	21.301332980773072	15.965305866685398
9	27.08963396562761	17.980172908630777	19.746636082089182	35.183557043652435
10	16.44685433277227	31.92945953516736	31.691730037787174	19.9319560942732
11	29.070242095016802	27.75408077766558	18.950475381470287	24.225201745847333
12	23.552803003385144	31.478280270040244	27.782028717253766	17.18688800932085
13	28.90795452636125	22.244517902359977	22.255508127119718	26.592019444159053
14	21.47097522676185	27.05979307387185	25.116545028316484	26.352686671049813
15	31.64494481924505	24.546738858285707	21.035646930208127	22.772669392261115
16	19.076873751381004	28.839218501197216	31.019765537137882	21.064142210283894
17	25.90667952072717	29.78261947390167	26.648175182481754	17.66252582288941
18	26.15914907775992	25.691556112808456	28.181538231013402	19.96775657841823
19	23.164455084911957	31.61323357344163	28.498775954502086	16.723535387144327
20	23.747967264698495	27.66280061345638	26.811503188474777	21.77772893337035
21	19.040942804231726	30.001245987067094	29.871354591930967	21.086456616770214
22	21.824496348658712	29.344368400102766	21.95766583197986	26.87346941925866
23	18.016437069246404	30.125061541150288	27.009384026150236	24.849117363453075
24	21.053636211142607	35.34055849924708	23.754410831854447	19.851394457755863
25	22.414839279966298	26.452675115241718	21.71488626557192	29.417599339220065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13533.0
1	13533.0
2	15995.0
3	18457.0
4	18457.0
5	18457.0
6	20674.5
7	22892.0
8	22892.0
9	22892.0
10	22691.5
11	22491.0
12	22491.0
13	22491.0
14	25732.5
15	28974.0
16	28974.0
17	28974.0
18	38518.0
19	48062.0
20	48062.0
21	48062.0
22	63457.5
23	78853.0
24	78853.0
25	78853.0
26	99572.0
27	120291.0
28	120291.0
29	120291.0
30	142943.5
31	165596.0
32	165596.0
33	165596.0
34	185469.5
35	205343.0
36	205343.0
37	205343.0
38	224274.5
39	243206.0
40	243206.0
41	243206.0
42	297974.5
43	352743.0
44	352743.0
45	352743.0
46	640621.5
47	928500.0
48	928500.0
49	928500.0
50	799973.0
51	671446.0
52	671446.0
53	671446.0
54	486386.0
55	301326.0
56	301326.0
57	301326.0
58	235097.5
59	168869.0
60	168869.0
61	168869.0
62	142746.0
63	116623.0
64	116623.0
65	116623.0
66	91438.5
67	66254.0
68	66254.0
69	66254.0
70	49853.5
71	33453.0
72	33453.0
73	33453.0
74	24215.5
75	14978.0
76	14978.0
77	14978.0
78	10411.0
79	5844.0
80	5844.0
81	5844.0
82	3952.5
83	2061.0
84	2061.0
85	2061.0
86	1318.5
87	576.0
88	576.0
89	576.0
90	377.0
91	178.0
92	178.0
93	178.0
94	108.0
95	38.0
96	38.0
97	38.0
98	24.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030298039055824965
2	0.014680777106140642
3	0.020051793120582338
4	0.02283370397934445
5	0.019335657651990114
6	0.005701540076868879
7	0.0
8	3.580677342961132E-4
9	2.7543671868931785E-4
10	2.7543671868931784E-5
11	0.0011017468747572714
12	7.712228123300899E-4
13	0.0027268235150242465
14	7.436791404611581E-4
15	0.002065775390169884
16	0.0023962994525970655
17	0.0026992798431553153
18	0.002478930468203861
19	3.8561140616504496E-4
20	6.059607811164993E-4
21	0.08130891935708663
22	0.07960121170121286
23	0.08108856998213516
24	0.0012945525778397938
25	0.22618863338766784
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3630598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.05529946217816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.11081707121151	41.38081101289913
2	9.25726767705669	8.897215408450036
3	2.266501954765579	3.2675229050361625
4	0.8187693515323462	1.573848255133613
5	0.41739297889443183	1.0028972297091268
6	0.23779325352525862	0.6856335604945174
7	0.1518634056924574	0.5108489006528106
8	0.10694187388047532	0.41112990194981847
9	0.08504447176152048	0.3678153802292359
>10	0.46933677879888136	4.198428133865691
>50	0.041024379728579044	1.3649389982349893
>100	0.027540097798275796	2.644675998034926
>500	0.003778058970143362	1.349629620815929
>1k	0.0035455630335028162	3.805296902272204
>5k	5.812398415578387E-4	2.067026238724345
>10k+	0.0018018435088293003	26.472281553497503
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	146982	4.048423978639332	No Hit
CCCATGTACTCTGCGTTGATACCAC	145272	4.001324299743459	No Hit
GAGTACATGGGAAGCAGTGGTATCA	70216	1.9340064639489143	No Hit
CATGTACTCTGCGTTGATACCACTG	59164	1.62959380245348	No Hit
GCGTTGATACCACTGCTTCCCATGT	48494	1.335702823611978	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	38397	1.0575943687513738	No Hit
GTATCAACGCAGAGTACATGGGAAG	37895	1.04376744547317	No Hit
GCTTCCCATGTACTCTGCGTTGATA	36570	1.0072720802468353	No Hit
ACTCTGCGTTGATACCACTGCTTCC	32760	0.9023306904262053	No Hit
TATCAACGCAGAGTACATGGGAAGC	29208	0.8044955679477596	No Hit
GGTATCAACGCAGAGTACATGGGAA	24372	0.6712943707896055	No Hit
CAGTGGTATCAACGCAGAGTACATG	21106	0.5813367384656742	No Hit
GTGGTATCAACGCAGAGTACATGGG	19006	0.5234950275409175	No Hit
GCAGTGGTATCAACGCAGAGTACAT	17039	0.46931662497472865	No Hit
ATACCACTGCTTCCCATGTACTCTG	16724	0.4606403683360152	No Hit
GTACATGGTAAGCAGTGGTATCAAC	15485	0.42651375889040866	No Hit
GTACTCTGCGTTGATACCACTGCTT	15366	0.4232360619380058	No Hit
CATGGGAAGCAGTGGTATCAACGCA	14577	0.4015041048334186	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	14555	0.4008981440523022	No Hit
ACATGGGAAGCAGTGGTATCAACGC	13944	0.3840689605403848	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	13301	0.3663583795286617	No Hit
TACCACTGCTTCCCATGTACTCTGC	12770	0.35173268976625893	No Hit
GATACCACTGCTTCCCATGTACTCT	12175	0.3353442050042445	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	11937	0.3287888110994387	No Hit
GTTGATACCACTGCTTCCCATGTAC	11917	0.32823793766206005	No Hit
CTTCCCATGTACTCTGCGTTGATAC	11911	0.3280726756308465	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	11838	0.32606198758441446	No Hit
CCACTGCTTCCCATGTACTCTGCGT	11481	0.31622889672720583	No Hit
ACCATGTACTCTGCGTTGATACCAC	11375	0.31330926750909904	No Hit
CCATGTACTCTGCGTTGATACCACT	11298	0.31118840477519133	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	10617	0.29243116423244875	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9984	0.2749960199394149	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	9575	0.26373065814502183	No Hit
TTCCCATGTACTCTGCGTTGATACC	9348	0.25747824463077434	No Hit
TGATACCACTGCTTCCCATGTACTC	8345	0.22985194174623574	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	7414	0.20420878323626027	No Hit
CCCCATGTACTCTGCGTTGATACCA	6763	0.18627785284958565	No Hit
ATCAACGCAGAGTACATGGGAAGCA	5968	0.1643806337137849	No Hit
GCTTACCATGTACTCTGCGTTGATA	5657	0.1558145517625471	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	5560	0.15314281559126072	No Hit
GAGTACATGGTAAGCAGTGGTATCA	5389	0.1484328477016734	No Hit
AAAAAGTACTCTGCGTTGATACCAC	4247	0.11697797442735329	No Hit
ACTCTGCGTTGATACCACTGCTTAC	4198	0.11562833450577564	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	4177	0.11504991739652806	No Hit
TCCATGTACTCTGCGTTGATACCAC	4018	0.11067047356936792	No Hit
GCGTTGATACCACTGCTTACCATGT	3949	0.10876996021041163	No Hit
GAACAAAAAAAAAAAAAAAAAAAAA	3885	0.10700716521079999	No Hit
CTGCGTTGATACCACTGCTTCCCAT	3717	0.10237982833681944	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.7543671868931784E-5	0.0
9	0.0	0.0	0.0	2.7543671868931784E-5	0.0
10	0.0	0.0	0.0	8.263101560679536E-5	0.0
11	0.0	0.0	0.0	8.263101560679536E-5	0.0
12	0.0	0.0	0.0	1.6526203121359072E-4	0.0
13	0.0	0.0	0.0	2.4789304682038606E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAAAA	340	0.0	12.852698	2
GGACCGT	70	1.09608685E-4	12.209009	6
TCGTGCA	55	0.003080389	12.085353	9
GCGCAAA	65	8.058039E-4	11.687095	11
ACGCAAA	405	0.0	11.027067	1
GATACGA	115	8.763527E-7	10.745146	18
GAACAAA	1620	0.0	10.616485	1
AGGACCG	100	2.4064122E-5	10.447787	5
GTACTAG	110	6.035716E-6	10.365869	1
ATACGAC	120	1.4812704E-6	10.3152075	19
GTACAAA	825	0.0	10.250692	1
CGACCAT	75	0.0026585408	10.128956	6
TATAACG	85	6.5986096E-4	10.058634	2
ACACCGT	85	6.623726E-4	10.054478	6
CTGTCGC	145	1.6299964E-7	9.82307	9
AGTACTC	1185	0.0	9.6182165	5
GCCTACG	80	0.004509882	9.502047	1
GTACGAC	80	0.004520146	9.499429	3
TACGACC	110	6.8511166E-5	9.498774	4
GGACCGC	90	0.0011186801	9.495896	6
>>END_MODULE