FastQCFastQC Report
Wed 25 May 2016
SRR1294725_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294725_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3630598
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1590254.380132418956877No Hit
GTACATGGGAAGCAGTGGTATCAAC1457484.014435087553069No Hit
GAGTACATGGGAAGCAGTGGTATCA764592.1059616074266554No Hit
CATGTACTCTGCGTTGATACCACTG673421.8548459509976043No Hit
GCGTTGATACCACTGCTTCCCATGT523431.4417184166354964No Hit
ACGCAGAGTACATGGGAAGCAGTGG469681.293671180339988No Hit
GTATCAACGCAGAGTACATGGGAAG409811.1287672168606935No Hit
GCTTCCCATGTACTCTGCGTTGATA405771.117639573425645No Hit
ACTCTGCGTTGATACCACTGCTTCC368251.014295716573413No Hit
TATCAACGCAGAGTACATGGGAAGC346830.9552971714301611No Hit
GGTATCAACGCAGAGTACATGGGAA277680.7648326804564978No Hit
CAGTGGTATCAACGCAGAGTACATG255620.7040713403136343No Hit
GTGGTATCAACGCAGAGTACATGGG217220.5983036403369362No Hit
GCAGTGGTATCAACGCAGAGTACAT198160.5458054017547522No Hit
CATGGGAAGCAGTGGTATCAACGCA188680.5196940008230049No Hit
ATACCACTGCTTCCCATGTACTCTG187650.5168570026205049No Hit
ACATGGGAAGCAGTGGTATCAACGC177350.4884870205955052No Hit
GGGAAGCAGTGGTATCAACGCAGAG162020.4462625716204328No Hit
CTGCTTCCCATGTACTCTGCGTTGA157610.4341158123262338No Hit
GTACATGGTAAGCAGTGGTATCAAC153030.4215008106102631No Hit
GTACTCTGCGTTGATACCACTGCTT152380.4197104719387825No Hit
AAGCAGTGGTATCAACGCAGAGTAC148460.40891335256616135No Hit
CTTCCCATGTACTCTGCGTTGATAC141790.3905417234295838No Hit
TACCACTGCTTCCCATGTACTCTGC140560.38715385178970513No Hit
GATACCACTGCTTCCCATGTACTCT139310.38371089280608867No Hit
GCAGAGTACATGGGAAGCAGTGGTA134620.37079291069955966No Hit
CCATGTACTCTGCGTTGATACCACT133520.3677631067939772No Hit
GTTGATACCACTGCTTCCCATGTAC130920.36060175210805495No Hit
ACCATGTACTCTGCGTTGATACCAC129140.35569897851538507No Hit
CCACTGCTTCCCATGTACTCTGCGT128070.35275180562540936No Hit
ATGGGAAGCAGTGGTATCAACGCAG119280.3285409180526183No Hit
GTACATGGGTAAGCAGTGGTATCAA108610.2991518201684681No Hit
TTCCCATGTACTCTGCGTTGATACC98070.270120790018614No Hit
GTATCAACGCAGAGTACTTTTTTTT97000.2671736171286383No Hit
TGATACCACTGCTTCCCATGTACTC93020.2562112357248035No Hit
GGTAAGCAGTGGTATCAACGCAGAG90000.24789304682038604No Hit
TATCAACGCAGAGTACTTTTTTTTT75520.20800980995417284No Hit
CCCCATGTACTCTGCGTTGATACCA74990.20654999534511945No Hit
ATCAACGCAGAGTACATGGGAAGCA72710.20027003815900302No Hit
GGTATCAACGCAGAGTACTTTTTTT71760.19765338933145446No Hit
GCTTACCATGTACTCTGCGTTGATA62990.17349758910240132No Hit
GAGTACATGGTAAGCAGTGGTATCA61600.1696690187126198No Hit
GTACTTTTTTTTTTTTTTTTTTTTT57450.1582383948870131No Hit
ACGCAGAGTACTTTTTTTTTTTTTT53900.1484603913735423No Hit
GGAAGCAGTGGTATCAACGCAGAGT51290.14127149301575112No Hit
AAAAAGTACTCTGCGTTGATACCAC47440.1306671793462124No Hit
ACTCTGCGTTGATACCACTGCTTAC45030.12402915442579981No Hit
CTGCGTTGATACCACTGCTTCCCAT43550.11995269098919793No Hit
TCCATGTACTCTGCGTTGATACCAC42390.11675762505240185No Hit
GCGTTGATACCACTGCTTACCATGT41880.11535289778708631No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA41060.11309431669383392No Hit
ATGGTAAGCAGTGGTATCAACGCAG40470.11146924005356693No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39990.1101471438038582No Hit
CTCTGCGTTGATACCACTGCTTCCC39850.10976153239769315No Hit
TGGGAAGCAGTGGTATCAACGCAGA38270.10540963224240193No Hit
ACGCAGAGTACATGGTAAGCAGTGG37700.10383964294587283No Hit
CTGCTTACCCATGTACTCTGCGTTG36580.10075475169655247No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACC753.2196112E-916.4922772
GTCGTAC801.2597775E-714.277681
GGACCGT759.737741E-713.9263646
TCTACGC604.041511E-412.6861923
AGACCGT907.5098396E-611.6053036
GACCGTT600.005904993511.0765697
AAAAGTA11050.010.6772142
TATACTG5000.010.6564025
TCTAGAC2100.010.4208013
CCACGTA1752.2919266E-1010.3303272
GAACAAA7650.010.2027871
TACGCAG750.00261539510.1489535
CTAGTAC750.00261539510.1489533
ATCTACG856.503872E-410.0744682
AGGACCG856.5046974E-410.0743285
CGCAAAA1803.8380676E-1010.0433742
ACTGATC2107.2759576E-129.9463078
TCTGTCG1054.135964E-59.9463078
GGCTTAG1451.5739715E-79.8466761
ACGCAAA1856.2755134E-109.7757091
Produced by FastQC (version 0.11.5)