FastQCFastQC Report
Wed 25 May 2016
SRR1294724_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294724_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3586348
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1066622.9741118262923734No Hit
CCCATGTACTCTGCGTTGATACCAC1022452.8509503260698628No Hit
GAGTACATGGGAAGCAGTGGTATCA500091.394426865435256No Hit
CATGTACTCTGCGTTGATACCACTG397481.1083140844112171No Hit
GCGTTGATACCACTGCTTCCCATGT348440.9715733107885793No Hit
GTATCAACGCAGAGTACATGGGAAG306330.8541558153308044No Hit
GCTTCCCATGTACTCTGCGTTGATA285670.796548466573796No Hit
ACGCAGAGTACATGGGAAGCAGTGG273420.7623911566864119No Hit
ACTCTGCGTTGATACCACTGCTTCC221250.616922841843569No Hit
TATCAACGCAGAGTACATGGGAAGC196640.5483015033677713No Hit
GGTATCAACGCAGAGTACATGGGAA174310.4860376070587685No Hit
CAGTGGTATCAACGCAGAGTACATG154140.42979655069725525No Hit
GTGGTATCAACGCAGAGTACATGGG148160.41312220676855677No Hit
GCAGTGGTATCAACGCAGAGTACAT124010.3457835101334282No Hit
ATACCACTGCTTCCCATGTACTCTG119830.3341281994943045No Hit
GTACTCTGCGTTGATACCACTGCTT111100.30978588803986673No Hit
CATGGGAAGCAGTGGTATCAACGCA107430.2995526368327892No Hit
CTGCTTCCCATGTACTCTGCGTTGA105250.29347402984874865No Hit
GTACATGGTAAGCAGTGGTATCAAC104490.29135488245981706No Hit
GGGAAGCAGTGGTATCAACGCAGAG103200.28775790860228845No Hit
ACATGGGAAGCAGTGGTATCAACGC95980.26762600840743844No Hit
TACCACTGCTTCCCATGTACTCTGC93620.2610454980944404No Hit
GATACCACTGCTTCCCATGTACTCT89150.2485815654253296No Hit
CCATGTACTCTGCGTTGATACCACT87600.24425962009264018No Hit
GCAGAGTACATGGGAAGCAGTGGTA85490.23837619773652755No Hit
AAGCAGTGGTATCAACGCAGAGTAC83480.232771610563169No Hit
CCACTGCTTCCCATGTACTCTGCGT80890.22554977932983636No Hit
CTTCCCATGTACTCTGCGTTGATAC80390.2241556034160656No Hit
GTTGATACCACTGCTTCCCATGTAC78530.2189692690168383No Hit
ACCATGTACTCTGCGTTGATACCAC73640.2053342285801601No Hit
ATGGGAAGCAGTGGTATCAACGCAG70490.19655092032340418No Hit
GTACATGGGTAAGCAGTGGTATCAA70240.19585383236651882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA60830.16961544166935277No Hit
TTCCCATGTACTCTGCGTTGATACC59500.1659069337387225No Hit
TGATACCACTGCTTCCCATGTACTC58200.1622820763629185No Hit
CCCCATGTACTCTGCGTTGATACCA53020.14783841389625324No Hit
ATCAACGCAGAGTACATGGGAAGCA49540.13813494953640862No Hit
GGTAAGCAGTGGTATCAACGCAGAG49240.13729844398814617No Hit
GCTTACCATGTACTCTGCGTTGATA38060.10612467055623157No Hit
GAGTACATGGTAAGCAGTGGTATCA37790.10537181556279536No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG701.09354674E-412.2120355
TACGTCG550.003074028312.0886825
GCTATAC1850.011.8089653
CCCCTAG1451.2187229E-911.1382091
GTATCGA1158.7702574E-710.744493518
CGCAAAA2800.010.5165062
GTGCGCA1002.4097344E-510.4464739
GTCTAAC1201.5248279E-610.29192351
ACTAGAC856.6069135E-410.0572513
GGTATCG1252.5561349E-69.88493417
CTAGTAC1356.463706E-79.8503123
GTACAAA7000.09.7716721
GTATAGG2950.09.6612591
CGCATAG1106.7019864E-59.51789319
TATCGAC1304.142041E-69.51789319
ACGCATA1106.8041205E-59.504745518
GTCTTAG2106.730261E-119.5002381
CCTAGAC2600.09.4985153
TAGACAG3100.09.498255
TTACACT4150.09.1551954
Produced by FastQC (version 0.11.5)