##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294724_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3586348
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.987303239953288	33.0	33.0	33.0	27.0	33.0
2	30.943579373780793	33.0	33.0	33.0	27.0	33.0
3	31.009535605579828	33.0	33.0	33.0	27.0	33.0
4	34.34708539160171	37.0	37.0	37.0	27.0	37.0
5	34.71525685739365	37.0	37.0	37.0	27.0	37.0
6	34.93915788428786	37.0	37.0	37.0	33.0	37.0
7	34.97064674147629	37.0	37.0	37.0	33.0	37.0
8	34.87249118044317	37.0	37.0	37.0	33.0	37.0
9	34.579415606070576	37.0	37.0	37.0	27.0	37.0
10	34.66840139328364	37.0	37.0	37.0	27.0	37.0
11	34.92269880112025	37.0	37.0	37.0	33.0	37.0
12	34.856527029724944	37.0	37.0	37.0	33.0	37.0
13	34.73022723952054	37.0	37.0	37.0	27.0	37.0
14	35.910306807928286	40.0	37.0	40.0	27.0	40.0
15	36.14885922950032	40.0	37.0	40.0	27.0	40.0
16	36.11757531617121	37.0	37.0	40.0	27.0	40.0
17	36.10653065458232	40.0	37.0	40.0	27.0	40.0
18	36.09317863185614	40.0	37.0	40.0	27.0	40.0
19	36.0838016834953	40.0	37.0	40.0	27.0	40.0
20	36.00891268778155	40.0	37.0	40.0	27.0	40.0
21	35.93399608738471	37.0	37.0	40.0	27.0	40.0
22	35.85243010438474	37.0	37.0	40.0	27.0	40.0
23	35.477606467637834	37.0	37.0	40.0	27.0	40.0
24	35.82369725414265	37.0	37.0	40.0	27.0	40.0
25	34.672626303972734	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6456063296684285
1101	2	0.7997121612499889
1101	3	0.6227579376243177
1101	4	0.6799936111907385
1101	5	0.6745481262646038
1101	6	0.5976202774363273
1101	7	0.5426040763927773
1101	8	0.613268090903766
1101	9	0.921668673068254
1101	10	0.9009494137342458
1101	11	0.6173336110900323
1101	12	0.6536162146375588
1101	13	0.6740423487996807
1101	14	1.4432713512148538
1101	15	1.2480436827883281
1101	16	1.204652424311334
1101	17	1.1559299624714399
1101	18	1.1114724728707372
1101	19	1.2125945681009327
1101	20	1.3511188379329155
1101	21	1.27188982318183
1101	22	1.2974778701725995
1101	23	1.6418360804831806
1101	24	1.4389529879800023
1101	25	1.312588352235224
1102	1	0.46782634177047555
1102	2	0.6264062951463671
1102	3	0.4565845505550534
1102	4	0.5019156790867072
1102	5	0.4453920701090155
1102	6	0.4995238708221095
1102	7	0.5183943864116003
1102	8	0.583631610266302
1102	9	0.7348126758473725
1102	10	0.7715173442043692
1102	11	0.5596888276844254
1102	12	0.5703282540241474
1102	13	0.5948285430948985
1102	14	1.3499387460957948
1102	15	1.0878502103688774
1102	16	0.8277365801153351
1102	17	0.9801105613941559
1102	18	1.0050105933759497
1102	19	0.9634990039035571
1102	20	1.056179676296992
1102	21	1.0467101545962336
1102	22	1.0971286790003845
1102	23	1.563682804225806
1102	24	1.2771850745593838
1102	25	1.1652098568154798
1103	1	0.4091182117465486
1103	2	0.5546875290840063
1103	3	0.3999619599476567
1103	4	0.23331588176348106
1103	5	0.36851318672537303
1103	6	0.3782017248294096
1103	7	0.3918111413215968
1103	8	0.481387544891561
1103	9	0.6851603994781215
1103	10	0.8235839936852685
1103	11	0.5299319842574732
1103	12	0.4771020841288447
1103	13	0.571576126167237
1103	14	1.1596213245414901
1103	15	0.8954997119166563
1103	16	0.6714331124937232
1103	17	0.6882072219045341
1103	18	0.7980854243351985
1103	19	0.8158209572593691
1103	20	1.01692656837281
1103	21	1.0810493761443496
1103	22	0.8629529004857517
1103	23	1.37007145657617
1103	24	1.01673606672162
1103	25	1.1084493892228835
1104	1	0.4565272525403721
1104	2	0.6217584588892251
1104	3	0.3545742348465879
1104	4	0.5683030188454339
1104	5	0.3486675417419107
1104	6	0.37691543302492647
1104	7	0.3712304724498523
1104	8	0.46852462684672247
1104	9	0.6101879628739297
1104	10	0.6530584516051547
1104	11	0.43842151016706765
1104	12	0.36501094116669464
1104	13	0.49844124928373645
1104	14	1.0089414274448387
1104	15	0.7981090467200431
1104	16	0.6758432558233807
1104	17	0.7596882950394104
1104	18	0.7547190149268914
1104	19	0.790095121169692
1104	20	0.9412191078803431
1104	21	0.8914132129690486
1104	22	0.9790866748334182
1104	23	1.4404499938786373
1104	24	1.1718261071479361
1104	25	0.9362700433941455
1105	1	0.2847241481556324
1105	2	0.3648342363871677
1105	3	0.33873113269928723
1105	4	0.22996489536692621
1105	5	0.2827761688178825
1105	6	0.22117648858396421
1105	7	0.2863907799780492
1105	8	0.3744205847726789
1105	9	-1.4123185343920923
1105	10	0.02074636231553484
1105	11	0.12766262989867272
1105	12	0.3110259681277583
1105	13	0.34961046170759147
1105	14	-1.4732736603138363
1105	15	-0.07545737634657712
1105	16	0.15128593044007488
1105	17	0.2663826463076333
1105	18	0.31976298843944306
1105	19	0.4749810262707683
1105	20	0.5551127582637392
1105	21	0.797957000509669
1105	22	0.7363282482304214
1105	23	1.1455179318828002
1105	24	0.8181156030146184
1105	25	0.6196917427685662
1106	1	-0.07803367116997251
1106	2	0.053504266907314246
1106	3	0.09963582280099814
1106	4	-1.1625420080960538
1106	5	-0.36378050128244155
1106	6	-0.21337545542857583
1106	7	-0.04468282188905448
1106	8	0.10946019561406217
1106	9	0.3042289736220738
1106	10	0.3532287577127988
1106	11	0.28177781011140013
1106	12	0.10675175012614346
1106	13	0.12030126437121424
1106	14	0.596479982082073
1106	15	0.3436764201869309
1106	16	0.24638826999394325
1106	17	0.13216073385016358
1106	18	0.19973439400836668
1106	19	0.28425770348391666
1106	20	0.3151852556375303
1106	21	0.44301890340911143
1106	22	0.5820728502602535
1106	23	0.7298174788687319
1106	24	0.4330244771683027
1106	25	0.3725218275719584
1107	1	0.3324095939573155
1107	2	0.322988243076864
1107	3	0.2812174817733073
1107	4	0.48556621381063536
1107	5	0.4217520244057127
1107	6	0.2501274708940002
1107	7	0.27978502696613106
1107	8	0.35023971563142453
1107	9	0.571605935836665
1107	10	0.5742438983209937
1107	11	0.3164331548904187
1107	12	0.3775693695468334
1107	13	0.36141004239764385
1107	14	0.8743761147658162
1107	15	0.6968416852032391
1107	16	0.5924503817784839
1107	17	0.6141538011991869
1107	18	0.6672786707072262
1107	19	0.6916261140868869
1107	20	0.8066095379826166
1107	21	0.7323161829706777
1107	22	0.6988717995455431
1107	23	0.9367988323449765
1107	24	0.837734500134097
1107	25	0.6591177676407511
1108	1	0.3883882974847417
1108	2	0.3582465358694549
1108	3	0.3831494474407897
1108	4	0.5266256320073026
1108	5	0.4226110350420953
1108	6	0.27927907308779965
1108	7	0.30489939543897293
1108	8	0.31574325421665606
1108	9	0.1505819552512122
1108	10	0.445673676386221
1108	11	0.26050838566396095
1108	12	0.38946278306599424
1108	13	0.30656827243441853
1108	14	-1.1444675098172468
1108	15	-0.11395959997839356
1108	16	0.2872488872807608
1108	17	0.3457234978933883
1108	18	0.4661889802901129
1108	19	0.42176786453175197
1108	20	0.535085251119348
1108	21	0.5885601922500783
1108	22	0.631187364864779
1108	23	0.8417011827553083
1108	24	0.765593096849102
1108	25	0.6402147703188632
1109	1	0.35926493854177366
1109	2	0.35312524865418027
1109	3	0.4046963841607294
1109	4	0.6011058083320222
1109	5	0.5639027235050378
1109	6	0.3723439640296462
1109	7	0.3011655760136449
1109	8	0.4231428448183081
1109	9	0.5333989590153436
1109	10	0.5733976016729159
1109	11	0.40790757049845894
1109	12	0.39293839810482467
1109	13	0.36276090070527545
1109	14	0.7812030752441643
1109	15	0.5351367090392571
1109	16	0.5406926075587677
1109	17	0.5328088126925437
1109	18	0.49470853211358445
1109	19	0.44522714652859463
1109	20	0.6696733127672019
1109	21	0.6222363074426198
1109	22	0.7854955885221742
1109	23	1.2944365590836284
1109	24	0.9575015574842638
1109	25	0.7843435388813376
1110	1	0.38796690252756605
1110	2	0.35916816549281805
1110	3	0.368717237994705
1110	4	0.5799674793119465
1110	5	0.4491459467475991
1110	6	0.34999584140329887
1110	7	0.339343262881151
1110	8	0.2895908854509983
1110	9	0.5231606078728106
1110	10	0.43151946806720787
1110	11	0.29510915548381433
1110	12	0.2757036579225698
1110	13	0.27910414001908634
1110	14	0.6818234470828415
1110	15	0.5512143721825211
1110	16	0.44625662588534
1110	17	0.3759650115966693
1110	18	0.518677039570882
1110	19	0.42679752715039854
1110	20	0.4613365311438926
1110	21	0.5977775643856944
1110	22	0.7204603946072581
1110	23	0.7813634021212081
1110	24	0.7057615281146212
1110	25	0.6090015975483638
1111	1	0.22737142854422387
1111	2	0.18679283592757656
1111	3	0.27992853137789453
1111	4	0.48521248907341885
1111	5	0.3826366349236281
1111	6	0.22665422559142456
1111	7	0.21281161828913753
1111	8	0.3584711362341082
1111	9	0.3441625037023428
1111	10	0.26416851549467424
1111	11	0.09484568995222986
1111	12	0.18894488477469196
1111	13	0.06571651296523129
1111	14	-1.9465646824962164
1111	15	-0.645406616897894
1111	16	-0.07262884264342517
1111	17	0.15385129554038457
1111	18	0.2965247081135445
1111	19	0.23196766269920488
1111	20	0.2910492914748346
1111	21	0.33480446666230534
1111	22	0.1523941296457565
1111	23	-1.9624830266454225
1111	24	-0.4182115545009282
1111	25	-0.10367418200296186
1112	1	-0.14268333922810328
1112	2	-0.1857241214158485
1112	3	0.017530515774204503
1112	4	0.1867573155797757
1112	5	-0.7035345601294267
1112	6	-0.9352649893539535
1112	7	-0.9555217985141056
1112	8	-0.7291437140410224
1112	9	-0.5735128824035414
1112	10	-0.9375016885690997
1112	11	-0.8483174338486137
1112	12	-0.8152538530451778
1112	13	-0.8206222263398288
1112	14	-0.544160893077553
1112	15	-0.9208029694356341
1112	16	-0.7930082507899741
1112	17	-0.8792776228680736
1112	18	-1.0599064755530634
1112	19	-0.8371150548276916
1112	20	-0.9969024606160843
1112	21	-1.8089938343509004
1112	22	-1.8014726857700438
1112	23	-1.4087802727717005
1112	24	-1.6937820137523616
1112	25	-2.036681263129381
1113	1	0.15412428503714892
1113	2	-0.03863515263196149
1113	3	-0.04664970450771122
1113	4	0.24904744854786998
1113	5	0.050873360888665786
1113	6	-0.08313122230492809
1113	7	0.03384360695299904
1113	8	0.08967612274491188
1113	9	0.25179785205973104
1113	10	-0.09193871419256539
1113	11	-0.11163193479023192
1113	12	-0.06184290743083665
1113	13	-0.030674728765212933
1113	14	-0.07887013236305762
1113	15	-0.1067859632152377
1113	16	0.027006586851982206
1113	17	-0.05998754696782527
1113	18	0.05981160924977047
1113	19	-0.2187660278345973
1113	20	-0.7546819504294717
1113	21	-0.6973211755596012
1113	22	-0.10624698240874153
1113	23	-0.3966835998275684
1113	24	-0.05188507829610245
1113	25	-1.2249652327692786
1114	1	-0.9820015982711325
1114	2	-0.6965525895837636
1114	3	-0.5581652801050794
1114	4	-1.706261558173047
1114	5	-0.8124354960945865
1114	6	-0.647387745972118
1114	7	-0.6537301820435886
1114	8	-0.49013589888642173
1114	9	-0.4882087709526388
1114	10	-0.627119580021855
1114	11	-0.6156806686430372
1114	12	-0.7103408082238758
1114	13	-0.788821033988178
1114	14	-0.69737344674882
1114	15	-1.5411316383881584
1114	16	-0.9699844346468964
1114	17	-0.9687194416676874
1114	18	-0.8887453227405331
1114	19	-0.9505197439204522
1114	20	-2.1066312469503785
1114	21	-6.891295443716732
1114	22	-7.955316938696864
1114	23	-8.098348740321818
1114	24	-6.153515433577521
1114	25	-7.400269747360067
1115	1	-1.2798501308708765
1115	2	-1.0527080068495138
1115	3	-0.9417603550887179
1115	4	-0.8047490876078527
1115	5	-1.0059227065132106
1115	6	-1.1804541443684329
1115	7	-1.3003470525096645
1115	8	-0.9662414012478777
1115	9	-2.9884328241793803
1115	10	-1.9153184294862768
1115	11	-1.455462023853464
1115	12	-1.3126186950606709
1115	13	-1.6122594868635503
1115	14	-1.3362293211721763
1115	15	-2.2813244873807363
1115	16	-1.5995636152056036
1115	17	-3.3121911835108904
1115	18	-3.093433154276248
1115	19	-2.4992733922043655
1115	20	-2.150446635994321
1115	21	-1.6142990701146545
1115	22	-1.1607646593633234
1115	23	-0.6372535058300812
1115	24	-1.6765639819055096
1115	25	-1.5306982157707836
1116	1	-1.7304715415110117
1116	2	-1.0108240869702634
1116	3	-0.8412708897173857
1116	4	-0.4362261330728998
1116	5	-0.4073758714930449
1116	6	-0.6070873493585083
1116	7	-0.9326599225084209
1116	8	-1.9994979445944665
1116	9	-1.1088279546868662
1116	10	-2.439737856711055
1116	11	-1.253690840041692
1116	12	-0.9282407113215143
1116	13	-0.9440464107725148
1116	14	-1.2521108708241684
1116	15	-1.350388581382731
1116	16	-1.0900090046801267
1116	17	-0.8763899231553083
1116	18	-1.3976454199531432
1116	19	-0.9314311492994207
1116	20	-1.2017518467790183
1116	21	-0.8284076236235833
1116	22	-0.44801097451625793
1116	23	-0.24205903815256136
1116	24	-0.3809570427306639
1116	25	-0.37344898880559185
1201	1	0.4541868752508904
1201	2	0.4637812834447068
1201	3	0.4256247217486617
1201	4	0.5213037217513161
1201	5	0.4457950443321934
1201	6	0.3721408528789638
1201	7	0.3794717467062938
1201	8	0.5104937665038562
1201	9	0.7663222483761771
1201	10	0.6509964075526469
1201	11	0.5342308079595739
1201	12	0.555664766646224
1201	13	0.531822513609562
1201	14	1.2464169443020694
1201	15	1.0197399332493262
1201	16	0.9600281877243333
1201	17	0.9029371115593818
1201	18	0.8440969903693301
1201	19	0.9116935343490624
1201	20	0.96115242817514
1201	21	1.0629669411241949
1201	22	1.0413563733847866
1201	23	0.8248202671508977
1201	24	0.8954605419647237
1201	25	0.9004701568595266
1202	1	-0.008117662702439077
1202	2	0.205555539463667
1202	3	0.20807558608956
1202	4	0.2002687420589666
1202	5	0.18662684146213593
1202	6	0.15410774253452786
1202	7	0.15164147308043852
1202	8	0.3044338798552104
1202	9	0.35571399174737905
1202	10	0.5148013841489458
1202	11	0.3519645635990045
1202	12	0.34836934946279996
1202	13	0.383639110007465
1202	14	0.6532492338805582
1202	15	0.6314181425113006
1202	16	0.5526357275883456
1202	17	0.4660067635426586
1202	18	0.5057775351105676
1202	19	0.5210663017790083
1202	20	0.6797127092373216
1202	21	0.7004486827055487
1202	22	0.6849702292390063
1202	23	1.011582550221732
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2211	7	0.22240649212861996
2211	8	0.34590580571082086
2211	9	0.41405859421939795
2211	10	0.34885498275225757
2211	11	0.13883919806079348
2211	12	0.22827647206941037
2211	13	0.19512726317353923
2211	14	0.39628103036134377
2211	15	0.23773973986388341
2211	16	0.11721490236960364
2211	17	0.225469817903587
2211	18	0.1926918249766203
2211	19	0.22908257569890367
2211	20	0.31742395184353
2211	21	0.32290487015123404
2211	22	0.43578349528029037
2211	23	0.4909244773871393
2211	24	0.5430530370584705
2211	25	0.5282752308382115
2212	1	-0.2715654262803078
2212	2	-0.2342755751435739
2212	3	-0.12463069717604114
2212	4	-0.48104569117118956
2212	5	-0.25639830294840493
2212	6	-0.6131135175382383
2212	7	-0.5256199070005749
2212	8	-0.21078685690408605
2212	9	-0.2621250816201055
2212	10	-0.455279730130016
2212	11	-0.40112785478997637
2212	12	-0.5510721177666298
2212	13	-0.6151351601924375
2212	14	-0.3768844163159031
2212	15	-0.6076268131705049
2212	16	-0.4619194293542037
2212	17	-0.5222414902303711
2212	18	-0.5074211673318842
2212	19	-0.5918198155481207
2212	20	-0.6867994721415229
2212	21	-0.5369411870044658
2212	22	-0.46207460121247124
2212	23	0.05499721484462583
2212	24	-0.18899689338245906
2212	25	-0.8498358457019819
2213	1	-0.20053664708141028
2213	2	-1.3806296329880254
2213	3	-0.3343971694868131
2213	4	-0.0547660259435645
2213	5	0.034201660940127
2213	6	0.014777477355181645
2213	7	0.024901048575408424
2213	8	0.0258112409139315
2213	9	0.07211607989208346
2213	10	-0.504720422454497
2213	11	-0.0410601765913583
2213	12	-0.022881177339165504
2213	13	-0.4084069807912485
2213	14	0.06474953249954041
2213	15	-0.023863605829603785
2213	16	-0.11621782399954839
2213	17	0.036046792456346566
2213	18	0.006417424104171232
2213	19	0.08822695384765211
2213	20	0.09983821945792926
2213	21	-0.5681818102055445
2213	22	-1.0654821497795055
2213	23	-0.15539355271830857
2213	24	0.0034136667766517803
2213	25	-0.6831474262622095
2214	1	-0.14031713108299826
2214	2	-0.39315606226487176
2214	3	-0.3875369088912102
2214	4	-0.5774610828094779
2214	5	-0.25244662889074476
2214	6	-0.1772569356172582
2214	7	-0.4981000094340189
2214	8	-0.08287533510932832
2214	9	-0.07895676850069577
2214	10	-2.0314072432786716
2214	11	-0.926681476283008
2214	12	-0.5701690011764029
2214	13	-0.37803724757394264
2214	14	-0.7358965071749708
2214	15	-0.4657820737112246
2214	16	-0.6516457142013081
2214	17	-0.39483182485443535
2214	18	-0.42795547475784446
2214	19	-1.5577087641148708
2214	20	-2.6563791369269296
2214	21	-1.9432738201384225
2214	22	-2.11711317779244
2214	23	-2.581942379602147
2214	24	-1.6694761409540249
2214	25	-1.9795361097530701
2215	1	-0.3658522396823223
2215	2	-0.4098788729944225
2215	3	-0.8909908707596479
2215	4	-0.39840571826746896
2215	5	-0.2135713313504084
2215	6	-0.20719784515107165
2215	7	-0.3637542849668094
2215	8	-0.31213335187830893
2215	9	-0.2742977392825807
2215	10	-0.3680473343155768
2215	11	-0.6112983510539038
2215	12	-0.5156328740959424
2215	13	-0.36661773078408544
2215	14	-0.16582233669190316
2215	15	-0.731801691653196
2215	16	-0.60558461461288
2215	17	-0.3415305771570374
2215	18	-0.42635722496780204
2215	19	-2.5493790847856275
2215	20	-2.694026051091477
2215	21	-3.5877400919406455
2215	22	-2.632236230730264
2215	23	-3.939992440618216
2215	24	-2.853704647289952
2215	25	-2.0176350764239146
2216	1	-1.9165385047235226
2216	2	-1.4868145289246364
2216	3	-1.1933434110268237
2216	4	-1.1429945079913821
2216	5	-2.1660642415180646
2216	6	-1.9891794642668401
2216	7	-1.4688525438971496
2216	8	-5.626662349951101
2216	9	-4.499001524770158
2216	10	-2.3151323266989436
2216	11	-1.3097851218218466
2216	12	-1.4372669048522013
2216	13	-2.4793202739047544
2216	14	-3.9247065770701433
2216	15	-2.379198867134228
2216	16	-3.286955915498339
2216	17	-3.0910059734633037
2216	18	-3.0350434768390357
2216	19	-1.9834142859129784
2216	20	-1.215552911239591
2216	21	-1.037485966448422
2216	22	-0.9366062453489263
2216	23	-1.363641750731432
2216	24	-0.8501251599528743
2216	25	-0.7194708601028097
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	57.0
8	231.0
9	697.0
10	1412.0
11	2754.0
12	4767.0
13	7254.0
14	10058.0
15	13876.0
16	17448.0
17	19446.0
18	20151.0
19	20681.0
20	21417.0
21	23404.0
22	24374.0
23	25915.0
24	27794.0
25	29895.0
26	32451.0
27	36415.0
28	41556.0
29	49306.0
30	59311.0
31	72942.0
32	98731.0
33	140203.0
34	203925.0
35	322988.0
36	555065.0
37	1701821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.19599346990598	18.990016587420616	11.393915119376713	24.420074823296694
2	13.780891628800154	21.979189267123314	36.98481416637989	27.25510493769664
3	21.536728565149584	27.459577828994192	26.796268242129194	24.20742536372703
4	13.455913953904117	18.628000288932466	33.3867040308031	34.52938172636032
5	12.879783002655573	38.93360396645979	34.21933331213783	13.967279718746811
6	33.358114207109175	33.0352285310584	17.788940015320268	15.817717246512156
7	25.53867405625733	32.92619202042436	22.194648197894992	19.340485725423317
8	26.548791998427347	35.34285383661969	21.02186239559211	17.08649176936086
9	26.584325994334023	17.660024760757544	20.08061188070222	35.675037364206204
10	16.58725405232979	30.716174631908032	31.512252630264015	21.184318685498162
11	30.338866978371048	26.924320972232135	20.044173611606826	22.69263843778999
12	23.655642035823522	29.655943739276907	28.328643731441545	18.35977049345803
13	28.380354758268545	22.445781456124507	23.294266777794107	25.879597007812848
14	21.737764132100835	25.27955528799569	25.51639967532187	27.466280904581602
15	29.357011781875936	25.711024069290918	21.60974449504792	23.32221965378523
16	20.495474393936856	29.07658675054235	29.068249373999656	21.359689481521137
17	25.02375041582398	29.213973470360287	26.817349473113023	18.944926640702704
18	25.20937427937186	26.559937718210037	27.811206093443317	20.419481908974785
19	23.154814916848235	29.96360628987707	28.34468289902725	18.53689589424744
20	23.877574351567336	27.33664404378838	26.844889218534774	21.940892386109514
21	20.62319255935131	29.03393962970332	28.957756084846704	21.385111726098668
22	22.32525601250581	28.433921733758154	23.59520305479329	25.645619198942743
23	19.603461473098964	29.454751851580035	27.009809516288406	23.931977159032595
24	21.64750680085079	32.707519359699475	24.714051968649724	20.930921870800006
25	22.924701037565644	26.453955649154814	23.133007822542965	27.48833549073658
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7793.0
1	7793.0
2	9429.5
3	11066.0
4	11066.0
5	11066.0
6	13246.0
7	15426.0
8	15426.0
9	15426.0
10	16500.0
11	17574.0
12	17574.0
13	17574.0
14	21418.0
15	25262.0
16	25262.0
17	25262.0
18	35498.5
19	45735.0
20	45735.0
21	45735.0
22	63248.5
23	80762.0
24	80762.0
25	80762.0
26	104383.5
27	128005.0
28	128005.0
29	128005.0
30	155212.5
31	182420.0
32	182420.0
33	182420.0
34	208763.0
35	235106.0
36	235106.0
37	235106.0
38	261200.5
39	287295.0
40	287295.0
41	287295.0
42	328210.5
43	369126.0
44	369126.0
45	369126.0
46	574061.5
47	778997.0
48	778997.0
49	778997.0
50	683599.0
51	588201.0
52	588201.0
53	588201.0
54	449402.0
55	310603.0
56	310603.0
57	310603.0
58	254154.5
59	197706.0
60	197706.0
61	197706.0
62	170360.5
63	143015.0
64	143015.0
65	143015.0
66	114743.0
67	86471.0
68	86471.0
69	86471.0
70	65407.5
71	44344.0
72	44344.0
73	44344.0
74	32227.0
75	20110.0
76	20110.0
77	20110.0
78	13809.0
79	7508.0
80	7508.0
81	7508.0
82	5105.0
83	2702.0
84	2702.0
85	2702.0
86	1748.5
87	795.0
88	795.0
89	795.0
90	521.5
91	248.0
92	248.0
93	248.0
94	152.5
95	57.0
96	57.0
97	57.0
98	39.0
99	21.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030699753621232517
2	0.014109060247360269
3	0.019295394646587558
4	0.02049438593243043
5	0.019016559463833405
6	0.006942996050578472
7	0.001171107767567453
8	0.001477826468597024
9	7.807385117116353E-4
10	4.461362924066488E-4
11	0.001226874804118284
12	0.0012547583223936998
13	0.002091263870656166
14	7.528549934362199E-4
15	0.0021470309072069973
16	0.002453749608236568
17	0.0026489342361644772
18	0.0018681957244528417
19	3.9036925585581765E-4
20	2.230681462033244E-4
21	0.08077855244387884
22	0.07623353896498611
23	0.07771136543358313
24	8.922725848132976E-4
25	0.22228740769161276
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3586348.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.96879584773337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.95437210355823	47.797771282283335
2	8.686670317166415	9.549916145217717
3	1.9515147508542465	3.2181724780064194
4	0.7509631874397663	1.6511816855815855
5	0.39430701320694655	1.0837290855151074
6	0.24507729324979802	0.8082962219737937
7	0.1761964348925823	0.677971410109616
8	0.12015740698471326	0.5283926379308571
9	0.09235646874330296	0.45690514880113686
>10	0.5498423364483708	5.637516824422254
>50	0.04596615700467233	1.7506116698066332
>100	0.025628067796740536	2.6985998778547065
>500	0.002110273843241187	0.8009604130971383
>1k	0.002985265436566792	3.276751400871949
>5k	8.235214997425633E-4	3.454654582690532
>10k+	0.001029401874678204	16.608569135837232
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	106662	2.9741118262923734	No Hit
CCCATGTACTCTGCGTTGATACCAC	102245	2.8509503260698628	No Hit
GAGTACATGGGAAGCAGTGGTATCA	50009	1.394426865435256	No Hit
CATGTACTCTGCGTTGATACCACTG	39748	1.1083140844112171	No Hit
GCGTTGATACCACTGCTTCCCATGT	34844	0.9715733107885793	No Hit
GTATCAACGCAGAGTACATGGGAAG	30633	0.8541558153308044	No Hit
GCTTCCCATGTACTCTGCGTTGATA	28567	0.796548466573796	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	27342	0.7623911566864119	No Hit
ACTCTGCGTTGATACCACTGCTTCC	22125	0.616922841843569	No Hit
TATCAACGCAGAGTACATGGGAAGC	19664	0.5483015033677713	No Hit
GGTATCAACGCAGAGTACATGGGAA	17431	0.4860376070587685	No Hit
CAGTGGTATCAACGCAGAGTACATG	15414	0.42979655069725525	No Hit
GTGGTATCAACGCAGAGTACATGGG	14816	0.41312220676855677	No Hit
GCAGTGGTATCAACGCAGAGTACAT	12401	0.3457835101334282	No Hit
ATACCACTGCTTCCCATGTACTCTG	11983	0.3341281994943045	No Hit
GTACTCTGCGTTGATACCACTGCTT	11110	0.30978588803986673	No Hit
CATGGGAAGCAGTGGTATCAACGCA	10743	0.2995526368327892	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	10525	0.29347402984874865	No Hit
GTACATGGTAAGCAGTGGTATCAAC	10449	0.29135488245981706	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	10320	0.28775790860228845	No Hit
ACATGGGAAGCAGTGGTATCAACGC	9598	0.26762600840743844	No Hit
TACCACTGCTTCCCATGTACTCTGC	9362	0.2610454980944404	No Hit
GATACCACTGCTTCCCATGTACTCT	8915	0.2485815654253296	No Hit
CCATGTACTCTGCGTTGATACCACT	8760	0.24425962009264018	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	8549	0.23837619773652755	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	8348	0.232771610563169	No Hit
CCACTGCTTCCCATGTACTCTGCGT	8089	0.22554977932983636	No Hit
CTTCCCATGTACTCTGCGTTGATAC	8039	0.2241556034160656	No Hit
GTTGATACCACTGCTTCCCATGTAC	7853	0.2189692690168383	No Hit
ACCATGTACTCTGCGTTGATACCAC	7364	0.2053342285801601	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	7049	0.19655092032340418	No Hit
GTACATGGGTAAGCAGTGGTATCAA	7024	0.19585383236651882	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	6083	0.16961544166935277	No Hit
TTCCCATGTACTCTGCGTTGATACC	5950	0.1659069337387225	No Hit
TGATACCACTGCTTCCCATGTACTC	5820	0.1622820763629185	No Hit
CCCCATGTACTCTGCGTTGATACCA	5302	0.14783841389625324	No Hit
ATCAACGCAGAGTACATGGGAAGCA	4954	0.13813494953640862	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	4924	0.13729844398814617	No Hit
GCTTACCATGTACTCTGCGTTGATA	3806	0.10612467055623157	No Hit
GAGTACATGGTAAGCAGTGGTATCA	3779	0.10537181556279536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.788351827541555E-5	0.0
10	0.0	0.0	0.0	1.115340731016622E-4	0.0
11	0.0	0.0	0.0	1.115340731016622E-4	0.0
12	0.0	0.0	0.0	2.230681462033244E-4	0.0
13	0.0	0.0	0.0	2.230681462033244E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGACCG	70	1.09354674E-4	12.212035	5
TACGTCG	55	0.0030740283	12.088682	5
GCTATAC	185	0.0	11.808965	3
CCCCTAG	145	1.2187229E-9	11.138209	1
GTATCGA	115	8.7702574E-7	10.7444935	18
CGCAAAA	280	0.0	10.516506	2
GTGCGCA	100	2.4097344E-5	10.446473	9
GTCTAAC	120	1.5248279E-6	10.2919235	1
ACTAGAC	85	6.6069135E-4	10.057251	3
GGTATCG	125	2.5561349E-6	9.884934	17
CTAGTAC	135	6.463706E-7	9.850312	3
GTACAAA	700	0.0	9.771672	1
GTATAGG	295	0.0	9.661259	1
CGCATAG	110	6.7019864E-5	9.517893	19
TATCGAC	130	4.142041E-6	9.517893	19
ACGCATA	110	6.8041205E-5	9.5047455	18
GTCTTAG	210	6.730261E-11	9.500238	1
CCTAGAC	260	0.0	9.498515	3
TAGACAG	310	0.0	9.49825	5
TTACACT	415	0.0	9.155195	4
>>END_MODULE