FastQCFastQC Report
Wed 25 May 2016
SRR1294724_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294724_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3586348
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1120493.1243203392420367No Hit
GTACATGGGAAGCAGTGGTATCAAC1037982.894253429951583No Hit
GAGTACATGGGAAGCAGTGGTATCA539741.5049850153972788No Hit
CATGTACTCTGCGTTGATACCACTG456581.273105677418923No Hit
GCGTTGATACCACTGCTTCCCATGT381171.0628360661040144No Hit
GTATCAACGCAGAGTACATGGGAAG327310.9126554366726263No Hit
ACGCAGAGTACATGGGAAGCAGTGG323070.9008328249238501No Hit
GCTTCCCATGTACTCTGCGTTGATA321150.8954791894149704No Hit
ACTCTGCGTTGATACCACTGCTTCC253480.7067914212452333No Hit
TATCAACGCAGAGTACATGGGAAGC225710.6293588909944043No Hit
GGTATCAACGCAGAGTACATGGGAA191590.5342203266386865No Hit
CAGTGGTATCAACGCAGAGTACATG182960.5101568503670029No Hit
GTGGTATCAACGCAGAGTACATGGG161690.450848606995194No Hit
GCAGTGGTATCAACGCAGAGTACAT142340.3968939991322649No Hit
ATACCACTGCTTCCCATGTACTCTG135880.37888124632634645No Hit
CATGGGAAGCAGTGGTATCAACGCA133820.3731372415616109No Hit
ACATGGGAAGCAGTGGTATCAACGC121400.33850591186354473No Hit
GGGAAGCAGTGGTATCAACGCAGAG119360.33281767413536No Hit
CTGCTTCCCATGTACTCTGCGTTGA115400.32177580089829544No Hit
GTACTCTGCGTTGATACCACTGCTT111650.3113194815450146No Hit
GATACCACTGCTTCCCATGTACTCT102980.2871444712002293No Hit
CCATGTACTCTGCGTTGATACCACT102640.2861964315788652No Hit
TACCACTGCTTCCCATGTACTCTGC102200.2849695567747469No Hit
GTACATGGTAAGCAGTGGTATCAAC99150.2764650837007452No Hit
AAGCAGTGGTATCAACGCAGAGTAC98580.27487572315904646No Hit
CTTCCCATGTACTCTGCGTTGATAC98130.27362096483665277No Hit
GTATCAACGCAGAGTACTTTTTTTT95480.26623183249366766No Hit
GCAGAGTACATGGGAAGCAGTGGTA94780.2642799862143886No Hit
CCACTGCTTCCCATGTACTCTGCGT91140.2541303855621373No Hit
GTTGATACCACTGCTTCCCATGTAC85870.2394357714309933No Hit
ACCATGTACTCTGCGTTGATACCAC83980.23416578647693975No Hit
ATGGGAAGCAGTGGTATCAACGCAG83980.23416578647693975No Hit
GTACATGGGTAAGCAGTGGTATCAA75030.20921003762044288No Hit
GGTATCAACGCAGAGTACTTTTTTT67750.18891083631594033No Hit
TTCCCATGTACTCTGCGTTGATACC65060.18141016989985356No Hit
TATCAACGCAGAGTACTTTTTTTTT64780.18062943138814194No Hit
TGATACCACTGCTTCCCATGTACTC63940.17828721585300703No Hit
CCCCATGTACTCTGCGTTGATACCA61110.1703961801810644No Hit
ATCAACGCAGAGTACATGGGAAGCA59070.16470794245287965No Hit
GGTAAGCAGTGGTATCAACGCAGAG58810.16398297097771883No Hit
ACGCAGAGTACTTTTTTTTTTTTTT46010.12829206758518694No Hit
GCTTACCATGTACTCTGCGTTGATA43820.12218557708287092No Hit
GTACTTTTTTTTTTTTTTTTTTTTT42150.11752902953087653No Hit
GAGTACATGGTAAGCAGTGGTATCA41060.11448972603885625No Hit
AAAAAGTACTCTGCGTTGATACCAC39370.109777411450311No Hit
GGAAGCAGTGGTATCAACGCAGAGT38250.10665445740346446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAACG601.4476627E-615.8546593
AATCCGT658.0585637E-411.6869976
CGCAAAA1701.364242E-1010.63194752
GTGCTAG1855.820766E-1110.288271
CAGTTCG750.002660556710.1280249
ATAGGAC2201.8189894E-129.9451943
GTCTAGC1151.00617945E-59.9304171
ATTAGAC1356.332248E-79.8651213
GTCCTAA2500.09.516651
CTAGGAC2600.09.5127953
TCTACGC800.00446799639.51279453
GCGTCAG1606.507071E-89.51279454
GAACGAA900.00111961459.49502316
CCGAATA1002.7716425E-49.4950239
AAAAGTA8400.09.28632
GTCTAAT1651.0385702E-79.2282671
TAGGACT3000.09.1957024
GGACAAT2700.09.1439926
AGGACGG1151.095192E-49.0991955
AGTACCG1151.095192E-49.0991955
Produced by FastQC (version 0.11.5)