FastQCFastQC Report
Wed 25 May 2016
SRR1294723_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294723_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569843
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC73830.20681581795053733No Hit
CCCATGTACTCTGCGTTGATACCAC68970.19320177385952267No Hit
GTATCAACGCAGAGTACTTTTTTTT44640.12504751609524564No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGT551.9682893E-413.81236610
GTCTAAC1605.4387783E-1010.68958951
TATTCCG1701.4006218E-1010.6152595
TAGACCG909.5438256E-510.5531825
CGCCCTA1451.43390935E-810.48818916
ATACCGT1303.8674807E-710.2266066
TCGGCAC750.002651491210.132193
CCGTGCG951.6521831E-49.99579059
TCGGAAC1054.1273124E-59.94819213
GTCCTAC2201.8189894E-129.9337611
GTCCAGA4950.09.7897921
GTATTAG4300.09.7228311
TATACTG10300.09.59007555
AACGATT1201.6911195E-59.50505417
AACTGCG800.00453411149.4958697
GTGCTAG3450.09.0887331
GAATCGT1151.1163E-49.0832596
TAGACGG950.00182840238.9979765
TAGACAG5700.08.9979765
GTGTAGA4700.08.8953561
Produced by FastQC (version 0.11.5)