##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294723_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3569843
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.993219029520347	33.0	33.0	33.0	27.0	33.0
2	30.92159319051286	33.0	33.0	33.0	27.0	33.0
3	30.92703768765181	33.0	33.0	33.0	27.0	33.0
4	34.374041659535166	37.0	37.0	37.0	27.0	37.0
5	34.58990745531386	37.0	37.0	37.0	27.0	37.0
6	34.79508370536183	37.0	37.0	37.0	27.0	37.0
7	34.70818548602838	37.0	37.0	37.0	27.0	37.0
8	34.6834322966024	37.0	37.0	37.0	27.0	37.0
9	34.31458106140802	37.0	37.0	37.0	27.0	37.0
10	34.38437936906469	37.0	37.0	37.0	27.0	37.0
11	34.6596382529988	37.0	37.0	37.0	27.0	37.0
12	34.57535107286231	37.0	37.0	37.0	27.0	37.0
13	34.510079855052446	37.0	37.0	37.0	27.0	37.0
14	35.5455105448615	37.0	37.0	40.0	27.0	40.0
15	35.88911529162487	37.0	37.0	40.0	27.0	40.0
16	35.89382306168647	37.0	37.0	40.0	27.0	40.0
17	35.81548180130051	37.0	37.0	40.0	27.0	40.0
18	35.81326517720807	37.0	37.0	40.0	27.0	40.0
19	35.89949838130136	37.0	37.0	40.0	27.0	40.0
20	35.793978614745804	37.0	37.0	40.0	27.0	40.0
21	35.737507223707034	37.0	37.0	40.0	27.0	40.0
22	35.58003391185551	37.0	37.0	40.0	27.0	40.0
23	35.17469227638302	37.0	33.0	40.0	27.0	40.0
24	35.50631498360012	37.0	33.0	40.0	27.0	40.0
25	34.23157545023689	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.563397215347134
1101	2	0.6113608138245752
1101	3	0.5044014153998191
1101	4	0.779931730930997
1101	5	0.6447381656317575
1101	6	0.5150177479936247
1101	7	0.5165589035444995
1101	8	0.5917213815933025
1101	9	0.7928520837239645
1101	10	0.849943129368917
1101	11	0.5511403074073726
1101	12	0.5769808682081745
1101	13	0.5708192316431919
1101	14	1.3618157865358143
1101	15	1.1478260328317518
1101	16	0.9772719087908257
1101	17	0.9708826448116739
1101	18	0.956447345699317
1101	19	1.008201843877515
1101	20	1.1445027494144853
1101	21	1.1535822323135037
1101	22	1.1619677898589913
1101	23	1.4862833381417175
1101	24	1.2708986975239824
1101	25	1.1043691582756878
1102	1	0.33465189857632893
1102	2	0.6021935240448393
1102	3	0.4201511866056329
1102	4	0.3809540914976992
1102	5	0.20581437322847762
1102	6	0.255653930567604
1102	7	0.25748746119074895
1102	8	0.455479951718587
1102	9	0.5953202223148537
1102	10	0.6558083450714349
1102	11	0.4868477584263715
1102	12	0.45916528404551116
1102	13	0.5205219519135156
1102	14	1.1947832408102101
1102	15	0.9218993669819326
1102	16	0.6061739230779324
1102	17	0.8955683065757185
1102	18	0.8023487947830432
1102	19	0.7912356817018704
1102	20	0.8383151917218825
1102	21	0.8302440757676166
1102	22	0.8666739972868385
1102	23	1.1775337886336033
1102	24	0.9991973131889225
1102	25	0.9267489124247916
1103	1	0.3935398338021301
1103	2	0.6114992865686872
1103	3	0.38160962415445
1103	4	0.2745216047012491
1103	5	0.47008026619939614
1103	6	0.4088653471434398
1103	7	0.30100802257652504
1103	8	0.5644237903236871
1103	9	0.7647023714970089
1103	10	0.7885103300363241
1103	11	0.5754457508192985
1103	12	0.5736106470488878
1103	13	0.384029594967366
1103	14	1.1611199283463023
1103	15	0.8100496057779978
1103	16	0.5118383307917185
1103	17	0.6871622637451296
1103	18	0.8095867465955635
1103	19	0.8387712817109971
1103	20	0.9345530785916907
1103	21	1.0070391710139859
1103	22	0.9245463690820444
1103	23	1.4105662792915084
1103	24	0.9550901612423104
1103	25	0.9837801884786614
1104	1	0.43137352178719013
1104	2	0.6121296981094453
1104	3	0.4779452342898409
1104	4	0.40351960500316864
1104	5	0.4321829890561304
1104	6	0.35919642435412413
1104	7	0.3165936735537258
1104	8	0.44290997544982247
1104	9	0.7015583374008187
1104	10	0.73662891812954
1104	11	0.5389378192350307
1104	12	0.41725783165183117
1104	13	0.5028762840918617
1104	14	0.9259399244999358
1104	15	0.7987085052616365
1104	16	0.7643993712137487
1104	17	0.727906991014855
1104	18	0.7453970585856879
1104	19	0.796592541376036
1104	20	0.8398539328577499
1104	21	0.9127003638432001
1104	22	1.0161249931987797
1104	23	1.4454825173775845
1104	24	1.1174021596375852
1104	25	0.8785953456524496
1105	1	0.2787196296541907
1105	2	0.3377851172933255
1105	3	0.3644339734694668
1105	4	0.26117669200074545
1105	5	0.35589795378540146
1105	6	0.2701835369639696
1105	7	0.285098095270925
1105	8	0.3519620096777558
1105	9	-1.5835712197954663
1105	10	0.011189773015381377
1105	11	0.2093380863150358
1105	12	0.3261432486647635
1105	13	0.34419975417853266
1105	14	-1.5018795281009574
1105	15	-0.16755899826667076
1105	16	0.10188520772450005
1105	17	0.25275214165363735
1105	18	0.22105596606101585
1105	19	0.3639920775563894
1105	20	0.48838651124273724
1105	21	0.5938891981916257
1105	22	0.6943932042346646
1105	23	1.0084659791826454
1105	24	0.7725802410198384
1105	25	0.491371295155119
1106	1	0.17730038381099789
1106	2	0.19652740253793155
1106	3	0.18257516513908456
1106	4	-1.0493769691566328
1106	5	-0.16739952055741014
1106	6	0.023892002542858393
1106	7	0.08609560460316601
1106	8	0.2222414903435066
1106	9	0.46831676019637314
1106	10	0.4117417364607405
1106	11	0.3746163558081861
1106	12	0.2135796621031858
1106	13	0.2192001164951236
1106	14	0.6819528704555751
1106	15	0.49349300348072944
1106	16	0.2785081272508805
1106	17	0.019748619936386547
1106	18	0.26442018947163604
1106	19	0.21140907082558158
1106	20	0.3316663666473616
1106	21	0.5902718293554088
1106	22	0.6516279419071012
1106	23	0.7673208931272768
1106	24	0.5035816012598318
1106	25	0.5287313115525336
1107	1	0.5082766612215792
1107	2	0.4365493923191508
1107	3	0.3540919728746488
1107	4	0.529872211686218
1107	5	0.5550342980126217
1107	6	0.2871310603588455
1107	7	0.34912782576425627
1107	8	0.37315656930261554
1107	9	0.5182174786020255
1107	10	0.5500641798966939
1107	11	0.33425452395358235
1107	12	0.3631169359582813
1107	13	0.42713642844427824
1107	14	1.0190679757889782
1107	15	0.6638297293551787
1107	16	0.6817741725256425
1107	17	0.642295789193021
1107	18	0.7372117829580205
1107	19	0.741057519907649
1107	20	0.8533922368942157
1107	21	0.8103940750681105
1107	22	0.7509551033186455
1107	23	0.8791939470778374
1107	24	0.9516285592701053
1107	25	0.7474397604834095
1108	1	0.44223803558686825
1108	2	0.35971794354523823
1108	3	0.4540890504231072
1108	4	0.6397593951701808
1108	5	0.5351192737287889
1108	6	0.39110124834761706
1108	7	0.3214035680442109
1108	8	0.3552366817272272
1108	9	0.20924854600194465
1108	10	0.44162291787059615
1108	11	0.27017599724563723
1108	12	0.3904163111428858
1108	13	0.35757599165205534
1108	14	-1.1712647285120354
1108	15	-0.07440774921532523
1108	16	0.33082859335842585
1108	17	0.42038514573918917
1108	18	0.6052574012006673
1108	19	0.4724579250725327
1108	20	0.5602890920318515
1108	21	0.6464276969437748
1108	22	0.7229787001177783
1108	23	1.0370169682370545
1108	24	0.9453091433839305
1108	25	0.8403911988693764
1109	1	0.5930486372911936
1109	2	0.4889405969137606
1109	3	0.5265818653463086
1109	4	0.7007385652856684
1109	5	0.5836721700790335
1109	6	0.4252527927615546
1109	7	0.4224537798176726
1109	8	0.5458022170643702
1109	9	0.6616283435451891
1109	10	0.6107256393643894
1109	11	0.42723814452375564
1109	12	0.44222831751677205
1109	13	0.41563554301745853
1109	14	0.8840002145466528
1109	15	0.6350079406218256
1109	16	0.6404855710282931
1109	17	0.6385815244617206
1109	18	0.5795442012701102
1109	19	0.6239175514752091
1109	20	0.7710557071788742
1109	21	0.7596745676666004
1109	22	0.8190306960268856
1109	23	1.2662196931235883
1109	24	1.0089447311497963
1109	25	0.7864818019481561
1110	1	0.4277331972190943
1110	2	0.30012732748773985
1110	3	0.40723108158494625
1110	4	0.6666308509687155
1110	5	0.5762336343682861
1110	6	0.3572590365501398
1110	7	0.239773815793626
1110	8	0.30414256137773066
1110	9	0.5644522310219173
1110	10	0.5257102899075292
1110	11	0.2597170623129088
1110	12	0.19811955147606852
1110	13	0.18062065710492448
1110	14	0.5919948867953337
1110	15	0.46226277525100556
1110	16	0.41885041626864705
1110	17	0.3809547314277566
1110	18	0.5101284691159691
1110	19	0.3320444413009156
1110	20	0.5519839832415627
1110	21	0.6440707876744369
1110	22	0.7451907863093581
1110	23	0.9313243893679015
1110	24	0.7896595619343145
1110	25	0.7037335833244427
1111	1	0.2491366776553079
1111	2	0.08552339302699963
1111	3	0.309943573922709
1111	4	0.5778554581515536
1111	5	0.3832302679331221
1111	6	0.27332787367903677
1111	7	0.27883302825928524
1111	8	0.340653770636294
1111	9	0.38533387055117174
1111	10	0.255587214882425
1111	11	0.24285069462739983
1111	12	0.23127784539067875
1111	13	0.17950207307429622
1111	14	-1.5833357210871526
1111	15	-0.5307315135907729
1111	16	0.018451714364502436
1111	17	0.22223047612237679
1111	18	0.225979003402351
1111	19	0.3054977618290451
1111	20	0.2796798715504636
1111	21	0.4431445351536567
1111	22	0.2484758913064482
1111	23	-1.8511702564212626
1111	24	-0.3657211634378186
1111	25	0.07871810192349216
1112	1	-0.11711770564024704
1112	2	-0.26993248867071173
1112	3	0.026422087124025495
1112	4	0.23811497674032722
1112	5	-0.6426070019527472
1112	6	-0.8943609694532526
1112	7	-1.0837906496420828
1112	8	-0.9327838848942633
1112	9	-0.8329812660028821
1112	10	-1.0472909240501522
1112	11	-1.0359225182346279
1112	12	-0.9689678988192014
1112	13	-1.024639104355444
1112	14	-0.7561514439766057
1112	15	-0.9900839424034018
1112	16	-1.0233857100446855
1112	17	-1.0379235068345594
1112	18	-1.131250104429327
1112	19	-0.927268978117695
1112	20	-1.0516696620830004
1112	21	-1.94025174510044
1112	22	-1.9355771367343166
1112	23	-1.4803384754310471
1112	24	-1.8276258339286997
1112	25	-2.056767324063159
1113	1	-0.0530327159212618
1113	2	-0.1750044557714645
1113	3	-0.1313914838149728
1113	4	0.12708002129139118
1113	5	0.08169046206369757
1113	6	-0.09792505444297461
1113	7	-0.05448975862083216
1113	8	0.14106806849846976
1113	9	0.23350262707456437
1113	10	-0.10435055393363513
1113	11	-0.17608701446491892
1113	12	-0.1374051846314046
1113	13	-0.29297358002370544
1113	14	-0.061326221221293054
1113	15	-0.14772616310250442
1113	16	-0.012933345411376251
1113	17	0.03318684271993533
1113	18	-0.0023947240317951923
1113	19	-0.23758426255908915
1113	20	-0.7401549259623721
1113	21	-0.6578665497611667
1113	22	-0.032904072992025135
1113	23	-0.32817946240842844
1113	24	-0.09116250288003869
1113	25	-1.0632786331789887
1114	1	-0.9649013420255663
1114	2	-0.6752512211629949
1114	3	-0.5549399292222361
1114	4	-1.4399862638330703
1114	5	-0.852726651306206
1114	6	-0.6218355744240114
1114	7	-0.5043310243419725
1114	8	-0.44016732054666363
1114	9	-0.5126754616552773
1114	10	-0.581395935614978
1114	11	-0.5852592858219623
1114	12	-0.5548789688047009
1114	13	-0.49229114890112413
1114	14	-0.594224298053085
1114	15	-1.4364243081703947
1114	16	-0.8658445690333139
1114	17	-0.9220697570412142
1114	18	-0.6695657952940834
1114	19	-0.8079686389716656
1114	20	-2.228190107539845
1114	21	-6.844939913386842
1114	22	-7.860736017166573
1114	23	-8.141555549701991
1114	24	-6.06684935952741
1114	25	-7.525263375232278
1115	1	-1.1629539104137372
1115	2	-1.135659962963807
1115	3	-1.0755648828932074
1115	4	-0.7444567285449963
1115	5	-0.8086908198370608
1115	6	-1.0378239343971956
1115	7	-1.2449519318149527
1115	8	-0.8728943703805783
1115	9	-3.026915785808427
1115	10	-1.8601831088939633
1115	11	-1.326739761829323
1115	12	-1.3502933626693903
1115	13	-1.5146730174107503
1115	14	-1.3309510506355977
1115	15	-2.3246083226934573
1115	16	-1.5525287004624246
1115	17	-3.3372137092955825
1115	18	-2.8892858408933435
1115	19	-2.3041571229528657
1115	20	-2.1323038208641023
1115	21	-1.584142414722578
1115	22	-1.0994251944133495
1115	23	-0.5078674470712272
1115	24	-1.6942798976723665
1115	25	-1.3156193692005118
1116	1	-1.6905725571541765
1116	2	-0.9442380420618584
1116	3	-0.5710414901476355
1116	4	-0.32920930461577314
1116	5	-0.3832186521823573
1116	6	-0.29403961868918316
1116	7	-0.7069561253808985
1116	8	-1.8579346433546036
1116	9	-0.8512291102766127
1116	10	-2.408074698039538
1116	11	-1.191504073440285
1116	12	-0.8277503671750139
1116	13	-0.8836595788319812
1116	14	-1.3099442981828417
1116	15	-1.3218299779722784
1116	16	-0.8786116161042372
1116	17	-0.8023876204700144
1116	18	-1.4134283194457566
1116	19	-0.8555444169343929
1116	20	-1.0893733733634434
1116	21	-0.5415533127390901
1116	22	-0.10479205087785459
1116	23	-0.03585744069376773
1116	24	-0.11379263485159896
1116	25	-0.17223776441131378
1201	1	0.385663822900721
1201	2	0.4349345875668149
1201	3	0.4804809544037276
1201	4	0.5713048565608432
1201	5	0.4527185673760954
1201	6	0.3694246398635528
1201	7	0.4148940621725714
1201	8	0.5685958895934533
1201	9	0.7702730523523726
1201	10	0.7577333851556673
1201	11	0.5384996773955137
1201	12	0.6314119700418672
1201	13	0.48164414659594
1201	14	1.266688423043469
1201	15	1.0399136459767249
1201	16	0.9677487143960235
1201	17	0.8250741818575946
1201	18	0.8061904361659984
1201	19	0.7755257252524146
1201	20	1.02755011433608
1201	21	0.9605526682672121
1201	22	1.0004333765340476
1201	23	0.7726695943293223
1201	24	0.9468139259886001
1201	25	0.9281470447114799
1202	1	-0.11045979186260269
1202	2	0.2455361397360285
1202	3	0.08809264305663689
1202	4	0.15676040895149157
1202	5	0.08220045296196332
1202	6	0.05981698630938581
1202	7	-0.014233149553795954
1202	8	0.2206856127009047
1202	9	0.31997024993442835
1202	10	0.5278849177699314
1202	11	0.301985632365934
1202	12	0.2215196947532334
1202	13	0.34609682655902674
1202	14	0.56747825030466
1202	15	0.6669564581037832
1202	16	0.5627644090047426
1202	17	0.32269670241507953
1202	18	0.37472530826118344
1202	19	0.3877468706517675
1202	20	0.561105216824771
1202	21	0.6009298588305967
1202	22	0.5853513714862402
1202	23	0.8439608960570482
1202	24	0.5388947135362798
1202	25	0.5993190828557076
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2211	9	0.38114501167892456
2211	10	0.36816749930602555
2211	11	0.2273645414922143
2211	12	0.21101830082061923
2211	13	0.08810035024637841
2211	14	0.45089914803298825
2211	15	0.2529296541140553
2211	16	0.12409744407403878
2211	17	0.2884602632216158
2211	18	0.35880177042740513
2211	19	0.2868314440883708
2211	20	0.33827119582476683
2211	21	0.33618963418761183
2211	22	0.5146087687532983
2211	23	0.6180528638156488
2211	24	0.5329495519815879
2211	25	0.6210895501806561
2212	1	-0.29686423355657254
2212	2	-0.39070801707848446
2212	3	-0.36535552577141317
2212	4	-0.6945417272841397
2212	5	-0.4104891735979592
2212	6	-0.6834028122474294
2212	7	-0.585386845944555
2212	8	-0.4267827120858456
2212	9	-0.28469964247641144
2212	10	-0.6386631553611011
2212	11	-0.570482990348097
2212	12	-0.7725847399844739
2212	13	-0.863951470918181
2212	14	-0.7074899961895653
2212	15	-0.9044278894340749
2212	16	-0.6033451567336741
2212	17	-0.5372358329904543
2212	18	-0.48547120457615023
2212	19	-0.5990289685816776
2212	20	-0.6489200778946014
2212	21	-0.5471116641958531
2212	22	-0.5517922485880149
2212	23	-0.1360111154066388
2212	24	-0.3173801755169876
2212	25	-1.029032703031298
2213	1	-0.19542156779814235
2213	2	-1.3248142975445631
2213	3	-0.31634011528554495
2213	4	-0.056677095082200424
2213	5	0.014867751151022901
2213	6	-0.023277729893401045
2213	7	0.0849064878668031
2213	8	0.22231337649381544
2213	9	0.23333647272665559
2213	10	-0.40450078943886325
2213	11	-0.010848913571429364
2213	12	0.09156721182132088
2213	13	-0.2632292110573644
2213	14	0.040967530986961265
2213	15	0.03145862542697131
2213	16	-0.046480422134031585
2213	17	0.130381654867314
2213	18	0.04345645328881176
2213	19	0.15256470309297754
2213	20	0.251532903020383
2213	21	-0.5082087021028983
2213	22	-0.8989784726837939
2213	23	-0.06941804657697048
2213	24	0.05357723317778351
2213	25	-0.7297081349740253
2214	1	-0.26473405480272305
2214	2	-0.46763683706591763
2214	3	-0.4652659400546746
2214	4	-0.4859531616040016
2214	5	-0.22303463757307895
2214	6	-0.22771112736270283
2214	7	-0.4058246429479553
2214	8	-0.035861372569669925
2214	9	-0.01788462197645657
2214	10	-2.009041717560571
2214	11	-0.9274323388991732
2214	12	-0.5923429589885316
2214	13	-0.3177655506981054
2214	14	-0.8736896406711736
2214	15	-0.6402894834279778
2214	16	-0.7375335668729974
2214	17	-0.5468941009479877
2214	18	-0.5566502736224734
2214	19	-1.485698532362484
2214	20	-2.572063482853089
2214	21	-1.8706372069908284
2214	22	-2.0202017633617757
2214	23	-2.407982899649511
2214	24	-1.5330563428431603
2214	25	-1.8884926978376768
2215	1	-0.4617602141176036
2215	2	-0.5845958818301291
2215	3	-1.1956885706117895
2215	4	-0.7003138821275741
2215	5	-0.35753470982627533
2215	6	-0.35449852176282093
2215	7	-0.3667336081021304
2215	8	-0.41201319908287104
2215	9	-0.5002383169851825
2215	10	-0.42338880519085365
2215	11	-0.9630312886860395
2215	12	-0.6481261106652738
2215	13	-0.49943857890509946
2215	14	-0.34452765816713793
2215	15	-0.8451712829836211
2215	16	-0.5657570241758805
2215	17	-0.3956052406973285
2215	18	-0.43269070384318553
2215	19	-2.479147709834109
2215	20	-2.6568814376937837
2215	21	-3.704966988166305
2215	22	-2.6630692240186704
2215	23	-3.785892963383862
2215	24	-2.732283808411097
2215	25	-2.026847579319856
2216	1	-1.683938818631038
2216	2	-1.2321968078060763
2216	3	-1.0341239753861267
2216	4	-1.1007538607588927
2216	5	-2.143525822217903
2216	6	-1.8175219092581472
2216	7	-1.2474325718814256
2216	8	-5.364561133544264
2216	9	-4.487262248701462
2216	10	-2.3226827185196655
2216	11	-1.3290228812726284
2216	12	-1.4237143995105441
2216	13	-2.319917893506826
2216	14	-3.780548482621864
2216	15	-2.272825110437566
2216	16	-3.3266833602683334
2216	17	-2.9328027428832115
2216	18	-3.033474422032775
2216	19	-1.8456529109457591
2216	20	-1.081336166313605
2216	21	-0.9167879874920288
2216	22	-0.866526027480873
2216	23	-1.1557853396886415
2216	24	-0.8238902006063284
2216	25	-0.5491994541810499
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	58.0
8	265.0
9	819.0
10	1753.0
11	3519.0
12	6166.0
13	9439.0
14	12549.0
15	16000.0
16	18086.0
17	19330.0
18	20675.0
19	21483.0
20	22646.0
21	24615.0
22	25944.0
23	28029.0
24	30122.0
25	32631.0
26	35466.0
27	40213.0
28	46205.0
29	54010.0
30	64947.0
31	79451.0
32	105992.0
33	146791.0
34	210051.0
35	324171.0
36	545225.0
37	1623189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.488521385677466	20.225360747843723	12.432865468661866	23.85325239781695
2	15.954256474393242	20.20148533658776	39.47270214933381	24.37155603968518
3	19.018005942207495	24.772039795530677	29.02326225174024	27.186692010521586
4	12.63754214877052	16.042311005109127	36.97794226017721	34.34220458594314
5	13.7177798456285	37.786609573812605	34.54226947457258	13.953341105986317
6	33.249458342367966	37.3105318862236	16.147644636735006	13.292365134673428
7	27.74874991245885	32.14673296449331	22.03302752293578	18.071489600112052
8	24.92659922096149	37.38491253674941	19.922488329129067	17.765999913160037
9	24.494709616477657	16.743221359081332	21.132960891976786	37.629108132464225
10	15.154991268452642	30.021704212040177	32.85019891792682	21.97310560158036
11	34.037163416111994	25.12050391604462	21.871359215821833	18.970973452021557
12	21.10547245731138	26.908256027501793	30.9469371427675	21.039334372419326
13	27.274851173509933	23.25993252769583	26.046729739049756	23.418486559744476
14	20.732159959841123	22.33630201626356	27.28498151587993	29.646556508015387
15	23.489422448517093	30.223428522285843	23.075026815400516	23.212122213796547
16	22.803655482818037	30.391530413380615	24.802668862145115	22.002145241656233
17	21.058754650172563	29.732755143202905	27.579529156021692	21.62896105060284
18	21.44162955857998	29.286348183291167	28.192294590772693	21.079727667356163
19	21.872666205393536	28.03604093192113	28.45219520257267	21.639097660112665
20	22.883209746578757	27.770897581311367	27.791542839470935	21.554349832638948
21	23.038803399250444	28.11738134808226	27.23535592771771	21.608459324949592
22	21.9751920755485	27.880124000374533	28.266882267486682	21.877801656590282
23	22.110776193648082	28.14699998430074	27.97528937093081	21.766934451120363
24	22.0850220348232	27.467838570408908	27.981956432341942	22.46518296242595
25	22.652128379421782	27.474667497746818	27.288937930105035	22.584266192726364
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2660.0
1	2660.0
2	2980.0
3	3300.0
4	3300.0
5	3300.0
6	4714.0
7	6128.0
8	6128.0
9	6128.0
10	9373.5
11	12619.0
12	12619.0
13	12619.0
14	20943.0
15	29267.0
16	29267.0
17	29267.0
18	45664.0
19	62061.0
20	62061.0
21	62061.0
22	87253.0
23	112445.0
24	112445.0
25	112445.0
26	147028.0
27	181611.0
28	181611.0
29	181611.0
30	219054.0
31	256497.0
32	256497.0
33	256497.0
34	292929.5
35	329362.0
36	329362.0
37	329362.0
38	359090.5
39	388819.0
40	388819.0
41	388819.0
42	396367.5
43	403916.0
44	403916.0
45	403916.0
46	410908.5
47	417901.0
48	417901.0
49	417901.0
50	401476.5
51	385052.0
52	385052.0
53	385052.0
54	357160.5
55	329269.0
56	329269.0
57	329269.0
58	293796.5
59	258324.0
60	258324.0
61	258324.0
62	220839.0
63	183354.0
64	183354.0
65	183354.0
66	146407.0
67	109460.0
68	109460.0
69	109460.0
70	83270.0
71	57080.0
72	57080.0
73	57080.0
74	41527.0
75	25974.0
76	25974.0
77	25974.0
78	17962.0
79	9950.0
80	9950.0
81	9950.0
82	6689.5
83	3429.0
84	3429.0
85	3429.0
86	2197.0
87	965.0
88	965.0
89	965.0
90	639.0
91	313.0
92	313.0
93	313.0
94	187.5
95	62.0
96	62.0
97	62.0
98	43.5
99	25.0
100	25.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030141381567760823
2	0.015434852457096854
3	0.0223819366846105
4	0.026247652907985027
5	0.025771441489163528
6	0.006863046918309853
7	0.0026051565853176177
8	0.001624721311273353
9	0.0017927959296809412
10	8.123606556366765E-4
11	0.0011765223288531176
12	0.0010924850196493234
13	0.002297019784903706
14	6.162736008278236E-4
15	0.0018488208024834707
16	0.002297019784903706
17	0.0023250322213049703
18	0.002549131712515088
19	3.641616732164412E-4
20	4.481989824202353E-4
21	0.08232855058331698
22	0.0772302871582868
23	0.07829475974153485
24	0.0010924850196493234
25	0.23006614016358704
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3569843.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.09652734250315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.2522980902284	51.72205160334489
2	13.481406959153286	17.821483673821753
3	4.213987186360175	8.355897578526397
4	1.686127666241752	4.45788733578796
5	0.8151611685447351	2.6939661232631957
6	0.44779246038503545	1.7758515960963752
7	0.2748019509337503	1.2714418264566087
8	0.1670617731687373	0.8833762446507604
9	0.12377393119661338	0.7362924324863455
>10	0.4975872416491235	5.632848472664802
>50	0.026598930631668155	1.2048979575000023
>100	0.011441776078197494	1.3412541203308062
>500	9.804327345222963E-4	0.42251685438906433
>1k	8.95177677189046E-4	1.2778908571273917
>5k	8.525501687514693E-5	0.4023433235537234
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	7383	0.20681581795053733	No Hit
CCCATGTACTCTGCGTTGATACCAC	6897	0.19320177385952267	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4464	0.12504751609524564	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8012436401264706E-5
2	0.0	0.0	0.0	0.0	2.8012436401264706E-5
3	0.0	0.0	0.0	2.8012436401264706E-5	2.8012436401264706E-5
4	0.0	0.0	0.0	2.8012436401264706E-5	2.8012436401264706E-5
5	0.0	0.0	0.0	2.8012436401264706E-5	2.8012436401264706E-5
6	0.0	0.0	0.0	2.8012436401264706E-5	2.8012436401264706E-5
7	0.0	0.0	0.0	2.8012436401264706E-5	2.8012436401264706E-5
8	0.0	0.0	0.0	5.602487280252941E-5	2.8012436401264706E-5
9	0.0	0.0	0.0	8.403730920379412E-5	2.8012436401264706E-5
10	5.602487280252941E-5	0.0	0.0	1.4006218200632352E-4	2.8012436401264706E-5
11	5.602487280252941E-5	0.0	0.0	1.9608705480885294E-4	2.8012436401264706E-5
12	5.602487280252941E-5	0.0	0.0	1.9608705480885294E-4	2.8012436401264706E-5
13	5.602487280252941E-5	0.0	0.0	1.9608705480885294E-4	2.8012436401264706E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGT	55	1.9682893E-4	13.812366	10
GTCTAAC	160	5.4387783E-10	10.6895895	1
TATTCCG	170	1.4006218E-10	10.615259	5
TAGACCG	90	9.5438256E-5	10.553182	5
CGCCCTA	145	1.43390935E-8	10.488189	16
ATACCGT	130	3.8674807E-7	10.226606	6
TCGGCAC	75	0.0026514912	10.13219	3
CCGTGCG	95	1.6521831E-4	9.9957905	9
TCGGAAC	105	4.1273124E-5	9.948192	13
GTCCTAC	220	1.8189894E-12	9.933761	1
GTCCAGA	495	0.0	9.789792	1
GTATTAG	430	0.0	9.722831	1
TATACTG	1030	0.0	9.5900755	5
AACGATT	120	1.6911195E-5	9.505054	17
AACTGCG	80	0.0045341114	9.495869	7
GTGCTAG	345	0.0	9.088733	1
GAATCGT	115	1.1163E-4	9.083259	6
TAGACGG	95	0.0018284023	8.997976	5
TAGACAG	570	0.0	8.997976	5
GTGTAGA	470	0.0	8.895356	1
>>END_MODULE