FastQCFastQC Report
Wed 25 May 2016
SRR1294723_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294723_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569843
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT131820.3692599366414714No Hit
GGTATCAACGCAGAGTACTTTTTTT106430.2981363606186603No Hit
TATCAACGCAGAGTACTTTTTTTTT89300.2501510570632938No Hit
GTACATGGGAAGCAGTGGTATCAAC77500.21709638210980148No Hit
CCCATGTACTCTGCGTTGATACCAC71860.2012973679794882No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56850.15925070094118984No Hit
GAGTACATGGGAAGCAGTGGTATCA40430.11325428037031321No Hit
ATTCCATTCCATTCCATTCCATTCC39360.11025694967537789No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGAC4100.010.2127143
TACGACT750.002611391710.1508194
TAGGACT3950.09.8777734
TATTCCG1752.5011104E-99.7882895
GGACCGC1752.6029738E-99.7656656
GGTCTAC1901.0095391E-99.52214051
TAGACTG4950.09.2280175
TCTACAC4750.09.2158753
ACACCGC1451.7216425E-69.1670046
AGGACCG1356.704857E-69.1639345
CTAGACT3550.09.1142914
AGTACTG5300.08.9777295
CGCCCTA1804.2720785E-88.96592516
TGGACCG1502.6417274E-68.8819675
CACACCG1951.6030754E-88.7843625
TCTATAC5700.08.6816223
ACTGATC5050.08.6481198
TGTGACG1651.061615E-68.63030310
TATACTG10850.08.595455
TATCGCG1000.0029226168.5447187
Produced by FastQC (version 0.11.5)