FastQCFastQC Report
Wed 25 May 2016
SRR1294722_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294722_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711767
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT61990.16700940549339438No Hit
GGTATCAACGCAGAGTACTTTTTTT43410.11695238413402566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTA2100.010.8549875
CTAGACA4150.010.299164
GTCGTAC951.6433709E-410.0011141
TACGACC951.6485188E-49.9980144
GTCCTAA2950.09.9841631
TTGGCGT2151.0913936E-119.7194
GTTGGCG2350.09.7009853
TTAGACT3200.09.4981144
ATCGGTC3400.09.49568314
TTCGCGG1106.875664E-59.49568314
ACTATCG3550.09.36219211
CGCACGA950.00183181318.99615212
GTCTAGA3700.08.9874881
CCTATAC5550.08.8998533
CTACGTC1502.711482E-68.8649064
TATCGGT3750.08.86275713
GACTGCG2054.1782187E-98.8011137
TATACAG6800.08.7997245
CTATCGG3900.08.76548212
TCTACAC5100.08.7538713
Produced by FastQC (version 0.11.5)