##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294722_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3711767
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.829027253057642	33.0	33.0	33.0	27.0	33.0
2	30.868018385852345	33.0	33.0	33.0	27.0	33.0
3	30.841716088321277	33.0	33.0	33.0	27.0	33.0
4	34.31891522285747	37.0	37.0	37.0	27.0	37.0
5	34.51390321644651	37.0	37.0	37.0	27.0	37.0
6	34.67720037383812	37.0	37.0	37.0	27.0	37.0
7	34.63076157528207	37.0	37.0	37.0	27.0	37.0
8	34.5713850034229	37.0	37.0	37.0	27.0	37.0
9	34.27172772428873	37.0	37.0	37.0	27.0	37.0
10	34.338337778206444	37.0	37.0	37.0	27.0	37.0
11	34.56574348551512	37.0	37.0	37.0	27.0	37.0
12	34.48282718177084	37.0	37.0	37.0	27.0	37.0
13	34.40384835578311	37.0	37.0	37.0	27.0	37.0
14	35.40867220383176	37.0	33.0	40.0	27.0	40.0
15	35.73626685080179	37.0	37.0	40.0	27.0	40.0
16	35.74905240549851	37.0	37.0	40.0	27.0	40.0
17	35.68716463075403	37.0	37.0	40.0	27.0	40.0
18	35.68211743894485	37.0	37.0	40.0	27.0	40.0
19	35.77701240406523	37.0	37.0	40.0	27.0	40.0
20	35.65285671218048	37.0	37.0	40.0	27.0	40.0
21	35.604059198758975	37.0	37.0	40.0	27.0	40.0
22	35.454855598425226	37.0	33.0	40.0	27.0	40.0
23	35.04835863889086	37.0	33.0	40.0	27.0	40.0
24	35.3592555782731	37.0	33.0	40.0	27.0	40.0
25	34.03005495765225	37.0	33.0	40.0	15.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6901270452312858
1101	2	0.8086836946926006
1101	3	0.6332188023956284
1101	4	0.8328501888865603
1101	5	0.8224654236345472
1101	6	0.7199622163200772
1101	7	0.7146887382236429
1101	8	0.7742339925231718
1101	9	1.0464593607619577
1101	10	0.9701034765489069
1101	11	0.7343330391251399
1101	12	0.7954231507721872
1101	13	0.822132030932849
1101	14	1.5472518948425389
1101	15	1.2722253230378442
1101	16	1.204236954408067
1101	17	1.1843542357074313
1101	18	1.1131463477041095
1101	19	1.1620228334272156
1101	20	1.2396862607139099
1101	21	1.2929926919286956
1101	22	1.3610841100813929
1101	23	1.7772951088577074
1101	24	1.4392533350890133
1101	25	1.4457275969672452
1102	1	0.34819764940069575
1102	2	0.579689725873763
1102	3	0.3770597711967838
1102	4	0.3938007594342352
1102	5	0.30605216859472506
1102	6	0.3861737728826071
1102	7	0.316689609716299
1102	8	0.41805901998452555
1102	9	0.7473267514127215
1102	10	0.7114762396031225
1102	11	0.5220664851859311
1102	12	0.5396635127707796
1102	13	0.6526492451235413
1102	14	1.2984002730998228
1102	15	0.9214348511938368
1102	16	0.6693579661255669
1102	17	0.950011505253137
1102	18	0.9782591554038973
1102	19	0.8671393648667731
1102	20	1.0311033475703866
1102	21	1.0425322598569835
1102	22	1.1526626981846846
1102	23	1.4206144470538433
1102	24	1.1909495894391142
1102	25	1.1863018192558386
1103	1	0.4089958354668255
1103	2	0.5745481596557553
1103	3	0.4648292886331582
1103	4	0.2711272450752631
1103	5	0.3979362692627362
1103	6	0.2705279220159156
1103	7	0.288478271140697
1103	8	0.41574241974122117
1103	9	0.6426397792441421
1103	10	0.772332944782967
1103	11	0.5618115200313483
1103	12	0.4736089190142536
1103	13	0.45363489250237166
1103	14	1.2014304404046925
1103	15	0.8588342276082983
1103	16	0.5836067784133334
1103	17	0.7253765815342348
1103	18	0.7527877561783285
1103	19	0.8218182334500526
1103	20	1.0099569563019557
1103	21	1.0714961090795256
1103	22	0.9699614485727821
1103	23	1.423887184933129
1103	24	1.1179445237219596
1103	25	0.9484752594132999
1104	1	0.3750629185468668
1104	2	0.6117824283574365
1104	3	0.32793601456009824
1104	4	0.32114834230976896
1104	5	0.3342021818753622
1104	6	0.23267717253187925
1104	7	0.24562953615902217
1104	8	0.4554511632758178
1104	9	0.673946565057264
1104	10	0.7475172961602183
1104	11	0.44581169518964714
1104	12	0.417121824171673
1104	13	0.4908586383632141
1104	14	0.8682177406761653
1104	15	0.7362600835807527
1104	16	0.7133278418595168
1104	17	0.7672547284928939
1104	18	0.6826711261050633
1104	19	0.7629535527558033
1104	20	0.9102747218352292
1104	21	0.9171175428751184
1104	22	0.9626742448263812
1104	23	1.398073200738736
1104	24	1.0902995955462629
1104	25	0.9866235596591224
1105	1	0.3683151324310856
1105	2	0.353521657425496
1105	3	0.42850401692983553
1105	4	0.5038190381016108
1105	5	0.4143733658811328
1105	6	0.34543010351953995
1105	7	0.3286446185909355
1105	8	0.4036271506129836
1105	9	-1.4027010179160797
1105	10	0.010095666005256021
1105	11	0.16122330825921694
1105	12	0.3390344436256356
1105	13	0.4259024484624092
1105	14	-1.4216931945144964
1105	15	-0.13682214455943154
1105	16	-0.008025720332241804
1105	17	0.31784514146626464
1105	18	0.2667153443546866
1105	19	0.35361108154069143
1105	20	0.5601055720043178
1105	21	0.6994479777783553
1105	22	0.7072129457504204
1105	23	1.1474343968861689
1105	24	0.9041892786921366
1105	25	0.49873323815784687
1106	1	0.0759687901011894
1106	2	0.14226702576001315
1106	3	0.1710124926963168
1106	4	-1.1740787938162782
1106	5	-0.12265642856088732
1106	6	0.06376629309484372
1106	7	0.1699859140467268
1106	8	0.2206072541252908
1106	9	0.5523443200740417
1106	10	0.6037061773333221
1106	11	0.43826383479117936
1106	12	0.3124293329661185
1106	13	0.3464398746602413
1106	14	0.775267903823412
1106	15	0.5889548225443946
1106	16	0.37927240074128576
1106	17	0.23737875821715448
1106	18	0.3039549223225322
1106	19	0.33890764284642927
1106	20	0.5469364210756282
1106	21	0.697165077058898
1106	22	0.716928242410475
1106	23	0.9001974254397709
1106	24	0.5853972587440808
1106	25	0.5490216684089546
1107	1	0.4382148323247037
1107	2	0.4056750829962894
1107	3	0.44951375645022296
1107	4	0.5658086827762929
1107	5	0.47286430128122703
1107	6	0.3666344227813667
1107	7	0.3780040997727738
1107	8	0.533021102253457
1107	9	0.6920371030462391
1107	10	0.6931799759633677
1107	11	0.4238125566232185
1107	12	0.4312116429642856
1107	13	0.37083197484005126
1107	14	0.9242888610128546
1107	15	0.692679533534097
1107	16	0.6708425399751121
1107	17	0.636717341377441
1107	18	0.778653855866601
1107	19	0.7169007381531287
1107	20	0.7480341328995692
1107	21	0.7951808522890005
1107	22	0.6968226168544049
1107	23	0.9959160013439288
1107	24	0.9489224124513598
1107	25	0.8565296625338661
1108	1	0.5311495701483437
1108	2	0.48788522409808266
1108	3	0.5559143724891911
1108	4	0.7420560483647733
1108	5	0.624752936341956
1108	6	0.48240575768947025
1108	7	0.4396371356874553
1108	8	0.5269551278890887
1108	9	0.22225892992177876
1108	10	0.5475606632712626
1108	11	0.44083519718321895
1108	12	0.44399060807020874
1108	13	0.43520493450602515
1108	14	-1.0600449873225415
1108	15	-0.013796238757500134
1108	16	0.43510194017265036
1108	17	0.49369288290507995
1108	18	0.7255692692588909
1108	19	0.6125001808104003
1108	20	0.6381102803040051
1108	21	0.7042842943847916
1108	22	0.8812205088447627
1108	23	1.090405771748678
1108	24	1.0139327798574485
1108	25	0.8373614759792503
1109	1	0.4683845061327361
1109	2	0.3375570007382649
1109	3	0.4276723971982115
1109	4	0.6023300449983182
1109	5	0.59972968402937
1109	6	0.3933328373131175
1109	7	0.38655220398648993
1109	8	0.41945637843005557
1109	9	0.5320900979500323
1109	10	0.5279747307117404
1109	11	0.37609651809169975
1109	12	0.3738430519006073
1109	13	0.3483433043728681
1109	14	0.7730932496982774
1109	15	0.556116963395489
1109	16	0.5070885622371151
1109	17	0.5810552403702403
1109	18	0.5696191893894706
1109	19	0.6450210585002623
1109	20	0.7334866594969753
1109	21	0.7397659353193546
1109	22	0.8214434585409052
1109	23	1.2561088789320891
1109	24	0.9122943284946743
1109	25	0.7378844975996017
1110	1	0.4260911498866484
1110	2	0.2619145045987139
1110	3	0.34401735983663784
1110	4	0.5814834550947907
1110	5	0.43472625251973795
1110	6	0.32516130506300556
1110	7	0.22455488975072768
1110	8	0.2642756432493769
1110	9	0.36418102890238657
1110	10	0.41350889111996025
1110	11	0.27990616928009615
1110	12	0.1017888475582609
1110	13	0.08776979643783989
1110	14	0.5649720214880247
1110	15	0.43995597155410593
1110	16	0.4103887220590039
1110	17	0.37306556462088025
1110	18	0.5376774412920042
1110	19	0.5273027151898759
1110	20	0.6065763624011922
1110	21	0.5707411190199565
1110	22	0.6900492321234211
1110	23	0.8224217893791419
1110	24	0.7706185764334066
1110	25	0.5956560225543797
1111	1	0.16742840607232523
1111	2	0.10443952808526191
1111	3	0.2525199559984159
1111	4	0.5113647981261451
1111	5	0.4068700753579719
1111	6	0.2955103697262018
1111	7	0.21288679925365983
1111	8	0.2796280612327564
1111	9	0.26032224892543354
1111	10	0.14022992947821677
1111	11	0.1370886617550866
1111	12	0.13192415136641245
1111	13	0.13822129704862363
1111	14	-1.7711287990108744
1111	15	-0.5233937025617195
1111	16	-0.06378173039970392
1111	17	0.10170012457744804
1111	18	0.2387888274108505
1111	19	0.1780710513309529
1111	20	0.33632237501074513
1111	21	0.36356048950062814
1111	22	0.14743844614242363
1111	23	-1.9880617274553245
1111	24	-0.4340477282875881
1111	25	-0.07273487610881801
1112	1	-0.11581112545936278
1112	2	-0.2866940687927979
1112	3	-0.07858159580868573
1112	4	0.11402226100346269
1112	5	-0.7116679627351274
1112	6	-0.8221141367645259
1112	7	-0.9403455791427575
1112	8	-0.6270857921942294
1112	9	-0.5752896227972215
1112	10	-0.8961104850823176
1112	11	-0.7318934834387107
1112	12	-0.7916993437578554
1112	13	-0.7814250116576957
1112	14	-0.5947197526880075
1112	15	-0.9288877226137657
1112	16	-0.8181148839074694
1112	17	-0.8583405959967791
1112	18	-0.9894427504681857
1112	19	-0.7716459034499508
1112	20	-0.9094646044881429
1112	21	-1.8297814107536894
1112	22	-1.5824127252111708
1112	23	-1.3424946492507033
1112	24	-1.7292353280129404
1112	25	-2.072615740286448
1113	1	-0.08265011314613702
1113	2	-0.24586706168083694
1113	3	-0.2190145822990388
1113	4	0.08746962077766085
1113	5	-0.036401865936817046
1113	6	-0.14553519532986314
1113	7	-0.08411593303554099
1113	8	-0.18931800880459093
1113	9	0.06201348778583338
1113	10	-0.2974724230788013
1113	11	-0.29689679641830224
1113	12	-0.13389112900107847
1113	13	-0.1919004655489971
1113	14	-0.07915284576006343
1113	15	-0.30259552748530183
1113	16	-0.10138000430145411
1113	17	-0.2321844709172467
1113	18	-0.06529491841497048
1113	19	-0.2628377926270602
1113	20	-0.7927379637814411
1113	21	-0.8165963289253355
1113	22	-0.3113240067867338
1113	23	-0.484836546347573
1113	24	-0.11863698185213423
1113	25	-1.2500921392263251
1114	1	-1.0678032079899857
1114	2	-0.7968473834975214
1114	3	-0.6034676405770973
1114	4	-1.6691624122360196
1114	5	-0.9792468981191149
1114	6	-0.7186403354976605
1114	7	-0.6275820171670574
1114	8	-0.5224892820680793
1114	9	-0.5134054925636704
1114	10	-0.5567232209493014
1114	11	-0.6558781025532383
1114	12	-0.578291308494407
1114	13	-0.6377554095139786
1114	14	-0.6475831036023507
1114	15	-1.4814333774170478
1114	16	-0.9359770914998364
1114	17	-0.8343855298775296
1114	18	-0.8044694630891129
1114	19	-0.9175667684011231
1114	20	-2.1836296843684835
1114	21	-7.091923171044844
1114	22	-8.003482565100352
1114	23	-8.317612896528232
1114	24	-6.219749878246343
1114	25	-7.598025668793952
1115	1	-1.2669078952590844
1115	2	-1.1659685658905516
1115	3	-1.1240426021668668
1115	4	-0.955215667215505
1115	5	-0.8758737568335491
1115	6	-1.1674626204848764
1115	7	-1.3922453504453856
1115	8	-1.0806392756434562
1115	9	-3.050402091993348
1115	10	-1.97102413307298
1115	11	-1.469153380021936
1115	12	-1.3241411831508714
1115	13	-1.7206026314400518
1115	14	-1.3683160942766648
1115	15	-2.2409046001965223
1115	16	-1.5885641263163635
1115	17	-3.4617107171944426
1115	18	-3.073906054699229
1115	19	-2.4993435285397467
1115	20	-2.094506972920918
1115	21	-1.5760579013926161
1115	22	-1.1425277329635293
1115	23	-0.5346367556505172
1115	24	-1.634032619713814
1115	25	-1.2917868543316189
1116	1	-1.949427833528766
1116	2	-0.9668742332387055
1116	3	-0.8436718959036433
1116	4	-0.5278531826130433
1116	5	-0.6105837857784664
1116	6	-0.6189367973158184
1116	7	-0.9515072941874223
1116	8	-2.093693352920802
1116	9	-1.1257273444114233
1116	10	-2.601859119924569
1116	11	-1.2617930199235232
1116	12	-0.9521263150205215
1116	13	-0.9610375269171172
1116	14	-1.4334879781221304
1116	15	-1.2503792627056214
1116	16	-1.075141079602254
1116	17	-0.9470593812455164
1116	18	-1.5623820103292942
1116	19	-1.1506000112792734
1116	20	-1.336748932462747
1116	21	-0.8369300469860903
1116	22	-0.3624687326056275
1116	23	-0.28355400961589794
1116	24	-0.33598902845866263
1116	25	-0.4165611161234182
1201	1	0.4438073206200137
1201	2	0.4581161034777175
1201	3	0.48436643090750664
1201	4	0.6562119929533949
1201	5	0.6340588306034576
1201	6	0.5251000993926596
1201	7	0.5330272259732567
1201	8	0.5996380561335783
1201	9	0.9400368622351394
1201	10	0.8064447618502371
1201	11	0.5824643013007034
1201	12	0.6590559574255792
1201	13	0.5879896417197585
1201	14	1.3488722743769301
1201	15	1.0871092918745546
1201	16	1.011004957159443
1201	17	0.9454775329445582
1201	18	0.8362377365483411
1201	19	0.8480917918025028
1201	20	1.0370548394144308
1201	21	1.0182591744602547
1201	22	1.1986908137634842
1201	23	0.9497263874289743
1201	24	1.0040359774867582
1201	25	1.0578038429653631
1202	1	-0.13045240662084368
1202	2	-0.03484368085815959
1202	3	0.05441948393078988
1202	4	0.009428823112045848
1202	5	0.09301325307831831
1202	6	0.0218402346556843
1202	7	0.07419902444685533
1202	8	0.23381138144792857
1202	9	0.25725890310658883
1202	10	0.577494314329627
1202	11	0.1832567872353863
1202	12	0.18797566944434863
1202	13	0.18909777144588702
1202	14	0.5971404693308244
1202	15	0.5942880397529962
1202	16	0.43255123264803075
1202	17	0.2716159776391862
1202	18	0.2541516096140697
1202	19	0.3539441531520495
1202	20	0.5033060638368667
1202	21	0.5679588405202125
1202	22	0.5056299917219818
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2211	5	0.33490422474218917
2211	6	0.27453143344411757
2211	7	0.35445691520300215
2211	8	0.32836797901588
2211	9	0.35295669134173835
2211	10	0.45269152418002534
2211	11	0.12366653941855787
2211	12	0.23119181931416222
2211	13	0.20821211052827238
2211	14	0.5034780790968156
2211	15	0.3785219038085046
2211	16	0.22889118879787418
2211	17	0.2950596536285488
2211	18	0.2432734602742741
2211	19	0.2700801651273821
2211	20	0.3671244087341421
2211	21	0.37237880453482575
2211	22	0.38468937075587917
2211	23	0.474983190787988
2211	24	0.49478576381910955
2211	25	0.636271544636621
2212	1	-0.40010716618914444
2212	2	-0.30927811428757224
2212	3	-0.21812358913689422
2212	4	-0.6193953637480476
2212	5	-0.2325733650457451
2212	6	-0.6227977492588579
2212	7	-0.5720392685433424
2212	8	-0.377053774333568
2212	9	-0.13958693973504666
2212	10	-0.6267484818514788
2212	11	-0.5098973648776592
2212	12	-0.6950351466899463
2212	13	-0.6818416689485787
2212	14	-0.43393403273498166
2212	15	-0.6889485035239034
2212	16	-0.5250916290716461
2212	17	-0.4695602492160873
2212	18	-0.47258008562502596
2212	19	-0.6067420391983518
2212	20	-0.6330857402598227
2212	21	-0.5061501326130085
2212	22	-0.6008911841983746
2212	23	-0.0756601568202413
2212	24	-0.31210366507210807
2212	25	-1.2310326042100712
2213	1	-0.27051056068109247
2213	2	-1.4101791281675027
2213	3	-0.4572361297176428
2213	4	-0.09060879454195714
2213	5	-0.0912414065504592
2213	6	-0.10851270119372458
2213	7	-0.13669124372441388
2213	8	0.0014180988585152932
2213	9	-7.152374725123423E-4
2213	10	-0.49723890352078826
2213	11	-0.26825884641814923
2213	12	-0.09933873382236413
2213	13	-0.48175882002264103
2213	14	-0.19564962632143335
2213	15	-0.21250563005991552
2213	16	-0.1177236555286143
2213	17	0.06284399921339201
2213	18	-0.09515185796796288
2213	19	-0.10174608443816169
2213	20	0.10543387034253016
2213	21	-0.6695066249602846
2213	22	-0.9776617515300501
2213	23	-0.216306432298218
2213	24	-0.20112007479949057
2213	25	-0.8239490891015961
2214	1	-0.33710429178082535
2214	2	-0.44789046655945697
2214	3	-0.4822237836706442
2214	4	-0.6501058874194641
2214	5	-0.44742511227394033
2214	6	-0.3857274157108108
2214	7	-0.5859885915875154
2214	8	-0.13478198510351547
2214	9	-0.1414494816121561
2214	10	-2.14391206132872
2214	11	-1.1000899592449684
2214	12	-0.6181479644401477
2214	13	-0.4050128327545295
2214	14	-0.9173980355503915
2214	15	-0.6349236538872773
2214	16	-0.9950815304625635
2214	17	-0.7270497566094889
2214	18	-0.5446959242772849
2214	19	-1.5188633065601707
2214	20	-2.61562481800361
2214	21	-2.1379106398345584
2214	22	-2.297330533230962
2214	23	-2.736017894292388
2214	24	-1.7678616128738156
2214	25	-2.2007836525577673
2215	1	-0.14227802566915315
2215	2	-0.23653502992944198
2215	3	-0.6334879680612993
2215	4	-0.11032063798522529
2215	5	-0.15621978256479707
2215	6	-0.09822964849983151
2215	7	-0.23940668772696938
2215	8	-0.176325403965933
2215	9	-0.1942976506027918
2215	10	-0.2947164424865818
2215	11	-0.5006866219856292
2215	12	-0.33719479495128013
2215	13	-0.1998172976512862
2215	14	-0.14041870963059466
2215	15	-0.6195151028568091
2215	16	-0.43769041094537187
2215	17	-0.2806574128575079
2215	18	-0.3182424187249353
2215	19	-2.0671349567094097
2215	20	-2.4661198117441927
2215	21	-3.436639670675639
2215	22	-2.6815210872262227
2215	23	-3.583749868462842
2215	24	-2.2695692148602262
2215	25	-1.540756203267172
2216	1	-1.9422724623311503
2216	2	-1.336703290886014
2216	3	-1.0542033572948384
2216	4	-1.2256243802305704
2216	5	-2.2084185017963307
2216	6	-1.8255664505810785
2216	7	-1.3664305020155396
2216	8	-5.801143214652349
2216	9	-4.752930790486722
2216	10	-2.5323349335812395
2216	11	-1.3489150790598785
2216	12	-1.427072241419161
2216	13	-2.2389298051557844
2216	14	-3.9493692799727995
2216	15	-2.446770456068734
2216	16	-3.2940593523101924
2216	17	-2.9994829081546897
2216	18	-3.0800497831435933
2216	19	-1.976541640719816
2216	20	-1.2620215127648038
2216	21	-0.9331252820424609
2216	22	-0.9775789219270479
2216	23	-1.1292199104880538
2216	24	-0.8842194049742957
2216	25	-0.7998356109117566
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	45.0
8	285.0
9	848.0
10	1983.0
11	3825.0
12	6896.0
13	10482.0
14	14212.0
15	17522.0
16	20205.0
17	21442.0
18	22701.0
19	23504.0
20	24596.0
21	26297.0
22	28469.0
23	30216.0
24	32243.0
25	35086.0
26	38212.0
27	42939.0
28	49193.0
29	58250.0
30	69430.0
31	85915.0
32	113604.0
33	157174.0
34	222808.0
35	340321.0
36	563069.0
37	1649994.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.63720348869957	19.30548327781235	12.755534199372665	24.301779034115427
2	16.597561691952762	19.27489894273161	38.96507441404029	25.162464951275332
3	19.594954186659955	23.269183249857115	28.23410275887845	28.90175980460448
4	13.045789810091177	15.866963582215678	36.07373019559994	35.013516412093196
5	14.144306329013645	37.27074136378861	34.33579968672758	14.249152620470168
6	33.85395519490719	36.28100324739795	15.952017290716883	13.913024266977981
7	28.949958335208002	31.605081892263172	20.530033269868504	18.91492650266032
8	25.80155263062182	36.37353119068691	19.134697374429685	18.690218804261587
9	24.87916126155616	16.97375776753108	20.294738486946237	37.852342483966524
10	15.422896965245933	29.773582231858843	32.286563246022716	22.51695755687251
11	34.36896677513337	24.245326582856872	21.94608024596564	19.43962639604412
12	22.167809390025774	26.16371751630701	29.603741849862075	22.06473124380514
13	28.086201141204914	22.370342789849694	25.31676262921671	24.226693439728685
14	21.46581032284241	22.22574856220073	26.396854959750783	29.911586155206077
15	23.449301320090935	30.126270504677894	22.762691306496137	23.661736868735037
16	23.137737108716397	29.40670755725014	24.49105448886681	22.964500845166654
17	21.855597665651228	28.823781238726525	26.430322070836027	22.890299024786223
18	22.124489385743377	28.412595178605205	27.40152371982048	22.06139171583094
19	22.697337137102455	27.83522735998073	27.495873248167918	21.971562254748896
20	23.13124115142298	27.546915138526114	27.455502855118404	21.866340854932506
21	23.47527272531182	26.96391883220181	27.160960510776892	22.399847931709473
22	22.914076123940553	26.987946090109364	27.371445272100004	22.726532513850078
23	23.151619468838057	26.975114068271917	27.33497206631611	22.53829439657392
24	22.962386414580315	26.980201220773846	27.223105903855437	22.834306460790398
25	23.050807093752372	27.07702808917925	27.14714910936584	22.72501570770254
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2689.0
1	2689.0
2	2958.0
3	3227.0
4	3227.0
5	3227.0
6	4563.5
7	5900.0
8	5900.0
9	5900.0
10	9094.0
11	12288.0
12	12288.0
13	12288.0
14	19983.5
15	27679.0
16	27679.0
17	27679.0
18	42511.5
19	57344.0
20	57344.0
21	57344.0
22	82427.5
23	107511.0
24	107511.0
25	107511.0
26	140229.0
27	172947.0
28	172947.0
29	172947.0
30	208412.5
31	243878.0
32	243878.0
33	243878.0
34	275608.0
35	307338.0
36	307338.0
37	307338.0
38	331193.0
39	355048.0
40	355048.0
41	355048.0
42	372935.0
43	390822.0
44	390822.0
45	390822.0
46	405760.5
47	420699.0
48	420699.0
49	420699.0
50	418797.5
51	416896.0
52	416896.0
53	416896.0
54	399429.5
55	381963.0
56	381963.0
57	381963.0
58	348682.0
59	315401.0
60	315401.0
61	315401.0
62	274677.0
63	233953.0
64	233953.0
65	233953.0
66	185853.0
67	137753.0
68	137753.0
69	137753.0
70	104225.0
71	70697.0
72	70697.0
73	70697.0
74	51014.5
75	31332.0
76	31332.0
77	31332.0
78	21309.0
79	11286.0
80	11286.0
81	11286.0
82	7474.0
83	3662.0
84	3662.0
85	3662.0
86	2379.5
87	1097.0
88	1097.0
89	1097.0
90	699.5
91	302.0
92	302.0
93	302.0
94	168.0
95	34.0
96	34.0
97	34.0
98	27.5
99	21.0
100	21.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03119807897424596
2	0.013605379863552858
3	0.019667182773056607
4	0.01947859334920538
5	0.016703634683965886
6	0.004849442327602999
7	0.0
8	5.388269252892221E-5
9	5.388269252892221E-5
10	0.0
11	7.81299041669372E-4
12	5.38826925289222E-4
13	0.002047542316099044
14	4.849442327602999E-4
15	0.0014278913520164385
16	0.0019667182773056608
17	0.0018320115459833552
18	0.0016434221221321272
19	2.69413462644611E-4
20	3.2329615517353325E-4
21	0.07831849359078843
22	0.07443893972870602
23	0.07554353492554894
24	6.196509640826053E-4
25	0.22011079898064723
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3711767.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.553412112236146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.11060096354436	45.32645985291914
2	13.783077485323018	16.416585873166504
3	4.590100827096948	8.200684985784616
4	2.0435282496324225	4.86796320053425
5	1.0307239197239084	3.0691563182628663
6	0.6146107656035878	2.1961300927564467
7	0.37857671758602035	1.5781874694948534
8	0.2484607507343526	1.1837348385758784
9	0.19951380174462408	1.069355489163892
>10	0.9116045551763698	9.753649381433927
>50	0.06309011540889982	2.5605885477500685
>100	0.024605256160907713	2.4728813774300713
>500	8.217776056555303E-4	0.35182177926425967
>1k	6.391603482813387E-4	0.784258043814845
>5k	4.565431059152404E-5	0.16854274964840407
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6199	0.16700940549339438	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4341	0.11695238413402566	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	2.6941346264461103E-5	0.0	0.0	0.0	0.0
4	2.6941346264461103E-5	0.0	0.0	0.0	0.0
5	2.6941346264461103E-5	0.0	0.0	0.0	0.0
6	2.6941346264461103E-5	0.0	0.0	0.0	0.0
7	2.6941346264461103E-5	0.0	0.0	0.0	0.0
8	2.6941346264461103E-5	0.0	0.0	0.0	0.0
9	2.6941346264461103E-5	0.0	0.0	0.0	0.0
10	2.6941346264461103E-5	0.0	0.0	2.6941346264461103E-5	0.0
11	2.6941346264461103E-5	0.0	0.0	8.082403879338331E-5	0.0
12	2.6941346264461103E-5	0.0	0.0	1.6164807758676662E-4	0.0
13	2.6941346264461103E-5	0.0	0.0	1.8858942385122774E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGTA	210	0.0	10.854987	5
CTAGACA	415	0.0	10.29916	4
GTCGTAC	95	1.6433709E-4	10.001114	1
TACGACC	95	1.6485188E-4	9.998014	4
GTCCTAA	295	0.0	9.984163	1
TTGGCGT	215	1.0913936E-11	9.719	4
GTTGGCG	235	0.0	9.700985	3
TTAGACT	320	0.0	9.498114	4
ATCGGTC	340	0.0	9.495683	14
TTCGCGG	110	6.875664E-5	9.495683	14
ACTATCG	355	0.0	9.362192	11
CGCACGA	95	0.0018318131	8.996152	12
GTCTAGA	370	0.0	8.987488	1
CCTATAC	555	0.0	8.899853	3
CTACGTC	150	2.711482E-6	8.864906	4
TATCGGT	375	0.0	8.862757	13
GACTGCG	205	4.1782187E-9	8.801113	7
TATACAG	680	0.0	8.799724	5
CTATCGG	390	0.0	8.765482	12
TCTACAC	510	0.0	8.753871	3
>>END_MODULE