FastQCFastQC Report
Wed 25 May 2016
SRR1294722_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294722_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3711767
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT165720.44647199029464946No Hit
GGTATCAACGCAGAGTACTTTTTTT125510.3381408369652513No Hit
TATCAACGCAGAGTACTTTTTTTTT109020.293714556975155No Hit
ACGCAGAGTACTTTTTTTTTTTTTT66680.17964489689142665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC1450.013.1278291
TAGGACG701.07751424E-412.2313964
TATACCG907.352779E-611.6273775
CGAATTA907.5145617E-611.60466615
TCGTACC1451.1914381E-911.1538342
GCGACGT600.00590320111.0770318
CGCACTC951.3405315E-511.0154095
GGTATCA39300.010.0504721
CGTACCC1909.640644E-1110.01400853
GTTCTAG3550.09.9198311
CTAGGAC3750.09.8938413
CTAGACA4050.09.8656534
GGACCGA1356.4885717E-79.8475766
TAGGATA4950.09.8015914
GTAGCAC3800.09.7636593
ACTAGAC2750.09.6862783
ACAACCG1002.7186584E-49.5133095
TAGGACA4500.09.5133094
AGACCGT1606.688606E-89.4958786
CCGAATT1304.2727315E-69.49472614
Produced by FastQC (version 0.11.5)