FastQCFastQC Report
Wed 25 May 2016
SRR1294721_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294721_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3822718
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC85610.22395060268636086No Hit
CCCATGTACTCTGCGTTGATACCAC81080.2121003955824102No Hit
GTATCAACGCAGAGTACTTTTTTTT51630.1350609697079408No Hit
ATTCCATTCCATTCCATTCCATTCC50960.13330829007004963No Hit
GAATGGAATGGAATGGAATGGAATG45650.11941764995482271No Hit
GAGTACATGGGAAGCAGTGGTATCA40680.10641642935733162No Hit
GGTATCAACGCAGAGTACTTTTTTT38870.10168157839526747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCG350.002177539716.27835812
AGACCGT1051.382432E-913.5656566
GTCGTAT905.4054544E-712.6679061
TCGCGCG1207.4760464E-1012.6609469
TATCGCG1201.0099029E-811.86963757
TATGCGG600.005898115711.07832810
TACACCG951.3647899E-510.9976225
GTACCGT1002.4121873E-510.4455546
CGCGCCT1752.382876E-1010.30962812
TGCACCG750.002653840210.1311435
GTCTTAG3600.010.0287591
TGTATCG1901.0004442E-109.9978385
TAGACAG5850.09.9038415
GTCCTAG3550.09.9023771
CTAGGAC3650.09.8893073
TACCGTG1252.5875597E-69.8755387
CGCGCGC1651.031367E-89.78345910
GCGCGCC1856.548362E-109.75235111
TTCCGTA2002.6557245E-109.4995622
TTGAACG900.00111583419.4985684
Produced by FastQC (version 0.11.5)