FastQCFastQC Report
Wed 25 May 2016
SRR1294721_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294721_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3822718
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT151700.396838061295654No Hit
GGTATCAACGCAGAGTACTTTTTTT120040.3140174085559019No Hit
TATCAACGCAGAGTACTTTTTTTTT102790.2688924477295997No Hit
GTACATGGGAAGCAGTGGTATCAAC91840.24024790737899054No Hit
CCCATGTACTCTGCGTTGATACCAC88700.23203385653872455No Hit
ACGCAGAGTACTTTTTTTTTTTTTT64200.1679433324665853No Hit
ATTCCATTCCATTCCATTCCATTCC63820.16694927535852763No Hit
GAATGGAATGGAATGGAATGGAATG51220.13398843440714173No Hit
GAGTACATGGGAAGCAGTGGTATCA48730.12747474440960593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT855.36014E-511.1697386
GTTCCGG600.00578393511.107461
ATTAACG951.3347153E-511.01975253
TGGCGTA803.7113426E-410.7066935
CCGTACA1553.7107384E-910.41212417
AGGACCG1303.752666E-710.2491415
GGCGTAC750.002662358810.1272296
GTCTAGA4650.09.8277991
TAGACAG7250.09.5826945
GTTGGCG1002.7079612E-49.517063
GACTACG1002.7768526E-49.4932837
AGACCGT1356.906146E-69.1426376
GTCCGGG1252.6865384E-59.1398531
CTAGCAC3550.09.114933
GACATCG1151.12001064E-49.0805327
TTGGCGT1054.4153637E-49.0638664
TGTGCGG1054.5271544E-49.04122310
GTCTTAG3900.09.032441
TTGCGGA950.00183061328.99681419
TATTCCG2651.8189894E-128.9783585
Produced by FastQC (version 0.11.5)