FastQCFastQC Report
Wed 25 May 2016
SRR1294720_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294720_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3208233
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC973353.0339130605538935No Hit
CCCATGTACTCTGCGTTGATACCAC938762.9260967018293247No Hit
GAGTACATGGGAAGCAGTGGTATCA458201.428200507880818No Hit
CATGTACTCTGCGTTGATACCACTG378901.1810239468268047No Hit
GCGTTGATACCACTGCTTCCCATGT303090.9447256480436428No Hit
GCTTCCCATGTACTCTGCGTTGATA250890.7820192610698786No Hit
ACGCAGAGTACATGGGAAGCAGTGG247740.7722007722007722No Hit
GTATCAACGCAGAGTACATGGGAAG230790.7193679511431994No Hit
ACTCTGCGTTGATACCACTGCTTCC208080.6485813218678319No Hit
TATCAACGCAGAGTACATGGGAAGC194830.6072813290057175No Hit
GGTATCAACGCAGAGTACATGGGAA160580.5005247436828933No Hit
CAGTGGTATCAACGCAGAGTACATG128810.40149827023161966No Hit
GTGGTATCAACGCAGAGTACATGGG116980.36462438981208656No Hit
GCAGTGGTATCAACGCAGAGTACAT106310.3313662068808594No Hit
ATACCACTGCTTCCCATGTACTCTG103240.32179707645922223No Hit
GTACTCTGCGTTGATACCACTGCTT96170.2997600236641167No Hit
GTACATGGTAAGCAGTGGTATCAAC96120.29960417463444833No Hit
CTGCTTCCCATGTACTCTGCGTTGA91170.284175120697281No Hit
CATGGGAAGCAGTGGTATCAACGCA90810.28305300768366887No Hit
ACATGGGAAGCAGTGGTATCAACGC87260.27198772657721554No Hit
GGGAAGCAGTGGTATCAACGCAGAG85630.26690704821002714No Hit
TACCACTGCTTCCCATGTACTCTGC81340.25353520146448216No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA79980.24929610785750286No Hit
GTTGATACCACTGCTTCCCATGTAC78560.24486999541492152No Hit
CTTCCCATGTACTCTGCGTTGATAC77910.24284395802923292No Hit
AAGCAGTGGTATCAACGCAGAGTAC76040.2370152043196364No Hit
GCAGAGTACATGGGAAGCAGTGGTA74270.23149814866937657No Hit
GATACCACTGCTTCCCATGTACTCT74140.23109294119223883No Hit
CCACTGCTTCCCATGTACTCTGCGT71740.22361218776815772No Hit
ACCATGTACTCTGCGTTGATACCAC70290.2190925659077754No Hit
CCATGTACTCTGCGTTGATACCACT65090.20288426682226632No Hit
ATGGGAAGCAGTGGTATCAACGCAG59780.18633309987148688No Hit
TTCCCATGTACTCTGCGTTGATACC56860.17723151653885488No Hit
TGATACCACTGCTTCCCATGTACTC50480.1573451803531726No Hit
GTACATGGGTAAGCAGTGGTATCAA48840.1522333321800505No Hit
CCCCATGTACTCTGCGTTGATACCA40350.1257701669423636No Hit
GGTAAGCAGTGGTATCAACGCAGAG39560.12330775227360356No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA36180.11277235786802267No Hit
ATCAACGCAGAGTACATGGGAAGCA35430.11043462242299733No Hit
GCTTACCATGTACTCTGCGTTGATA34770.10837741523137502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG701.0902532E-412.2158991
GGTATCG1103.7880454E-812.09797817
GTATCGA1103.788955E-812.0977918
CTAGACT1353.7289283E-1011.9617454
TATCGAC1209.740688E-911.89827919
TTACGCT658.034364E-411.6911184
CTAGGAC2050.011.121343
CCGTATA700.001492060310.8572232
AGAAACG803.7581212E-410.692080515
CTTAGAC1605.456968E-1010.6869133
CGCAAAA2200.010.3637122
TAGACAG3000.010.1318285
ATCGTCG750.002659017510.1286719
GTCTCGC951.6428696E-410.0013211
GCTCTAG1054.101952E-59.9536951
CACACCG1252.5770714E-69.8785325
ACTGATC2900.09.8230648
AAGCGGT1752.5956979E-99.76723710
CCGGTAC900.00111475379.4994783
GCTAGAC1304.2449374E-69.4994773
Produced by FastQC (version 0.11.5)