##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294720_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3208233
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.751366873914705	33.0	33.0	33.0	27.0	33.0
2	30.785818237017075	33.0	33.0	33.0	27.0	33.0
3	30.773109372043738	33.0	33.0	33.0	27.0	33.0
4	34.15568944026197	37.0	33.0	37.0	27.0	37.0
5	34.4836808922544	37.0	37.0	37.0	27.0	37.0
6	34.63816187914033	37.0	37.0	37.0	27.0	37.0
7	34.68229676585211	37.0	37.0	37.0	27.0	37.0
8	34.639163053306916	37.0	37.0	37.0	27.0	37.0
9	34.298727991389654	37.0	37.0	37.0	27.0	37.0
10	34.4225696824389	37.0	37.0	37.0	27.0	37.0
11	34.650491719273504	37.0	37.0	37.0	27.0	37.0
12	34.59819720076441	37.0	37.0	37.0	27.0	37.0
13	34.445846358415984	37.0	37.0	37.0	27.0	37.0
14	35.54400506447007	37.0	37.0	40.0	27.0	40.0
15	35.782423221754776	37.0	37.0	40.0	27.0	40.0
16	35.74889573170029	37.0	37.0	40.0	27.0	40.0
17	35.7441055559244	37.0	37.0	40.0	27.0	40.0
18	35.71649471843223	37.0	37.0	40.0	27.0	40.0
19	35.69835763175555	37.0	37.0	40.0	27.0	40.0
20	35.61966540460122	37.0	37.0	40.0	27.0	40.0
21	35.53689460834048	37.0	37.0	40.0	27.0	40.0
22	35.44301426984885	37.0	33.0	40.0	27.0	40.0
23	35.06441832622506	37.0	33.0	40.0	22.0	40.0
24	35.404259291641225	37.0	33.0	40.0	27.0	40.0
25	34.14014100596808	37.0	33.0	40.0	15.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6423784217290169
1101	2	0.8519638580414366
1101	3	0.6499203468822934
1101	4	0.7640738007418193
1101	5	0.7477944181010869
1101	6	0.5601065897524222
1101	7	0.5712657517914295
1101	8	0.7764825575561787
1101	9	1.0167001816406156
1101	10	1.0408071167557083
1101	11	0.7357431209227769
1101	12	0.822003699634692
1101	13	0.8194790278744293
1101	14	1.5586209243950222
1101	15	1.2601381408943482
1101	16	1.1452777511875496
1101	17	1.2235487032566397
1101	18	1.1995838217548993
1101	19	1.268921990640635
1101	20	1.3767262534002427
1101	21	1.324856207646377
1101	22	1.3953945100290426
1101	23	1.6749630558964412
1101	24	1.4179314901081668
1101	25	1.419606492613262
1102	1	0.253734109688768
1102	2	0.5180185052723374
1102	3	0.2633669186017258
1102	4	0.19507169390272594
1102	5	0.262124648345214
1102	6	0.28209638937740067
1102	7	0.3379421510553655
1102	8	0.3854452220868865
1102	9	0.6557939557517827
1102	10	0.7246694742268005
1102	11	0.5086487196338396
1102	12	0.5164338644941608
1102	13	0.5098371813233058
1102	14	1.0973021130164398
1102	15	0.8259393880768755
1102	16	0.4740892585201024
1102	17	0.78732858961056
1102	18	0.8497687564300165
1102	19	0.7259697858165168
1102	20	0.8626480897620254
1102	21	0.8313081839460423
1102	22	0.9025082876163566
1102	23	1.2516192144234353
1102	24	0.9777751002978761
1102	25	0.8099192630376919
1103	1	0.28827477939907453
1103	2	0.5301053145215455
1103	3	0.2370517239872072
1103	4	0.029380117770834602
1103	5	0.36442791763159477
1103	6	0.21545806650334498
1103	7	0.17109155654634378
1103	8	0.30779920248797765
1103	9	0.574950258232299
1103	10	0.5877830330769171
1103	11	0.3901482046223492
1103	12	0.4793329589230737
1103	13	0.4087228894689616
1103	14	1.1072848400872388
1103	15	0.7180963105383711
1103	16	0.5675060133832517
1103	17	0.6741790595909762
1103	18	0.7363814940907076
1103	19	0.7057581169721772
1103	20	0.9299841337581611
1103	21	0.8836629408461363
1103	22	0.7068074390667363
1103	23	1.1843223788892203
1103	24	0.8235002251569696
1103	25	0.7912481393299444
1104	1	0.3818369944026898
1104	2	0.5587676954562291
1104	3	0.3795645456649446
1104	4	0.408446585570573
1104	5	0.404630147737123
1104	6	0.3036551725334746
1104	7	0.22301299947043418
1104	8	0.4821375167296722
1104	9	0.558650094349602
1104	10	0.6249573964705561
1104	11	0.4473479196538648
1104	12	0.4516542768615963
1104	13	0.45546580888325394
1104	14	0.9470994468797116
1104	15	0.6950271604248712
1104	16	0.6967538034747349
1104	17	0.6850883348055845
1104	18	0.5664329059798021
1104	19	0.6827459973206018
1104	20	0.7393588737454309
1104	21	0.7938207051364827
1104	22	0.9000920781022117
1104	23	1.258845173325831
1104	24	1.009917398652874
1104	25	0.8673020900593897
1105	1	0.3688118199611168
1105	2	0.3742867838863049
1105	3	0.37905604452035746
1105	4	0.30142458094816504
1105	5	0.28259869147174754
1105	6	0.22065944908789703
1105	7	0.28065984837216007
1105	8	0.3749908925475083
1105	9	-1.689162202181116
1105	10	-0.0818948984220782
1105	11	0.19207217188488812
1105	12	0.35014228929447455
1105	13	0.2173343486259256
1105	14	-1.625144494983651
1105	15	-0.16017760108331203
1105	16	-0.12772696700021413
1105	17	0.19601338790547373
1105	18	0.16236325358276815
1105	19	0.4013792498283095
1105	20	0.543741395656447
1105	21	0.6750263892681829
1105	22	0.6402620596979176
1105	23	0.9832866781003489
1105	24	0.7367259007610372
1105	25	0.35392615693978513
1106	1	0.10699533944286443
1106	2	0.20205451563274934
1106	3	0.03535253536374228
1106	4	-1.2018786975583566
1106	5	-0.4089392464442412
1106	6	-0.1286146345567687
1106	7	0.056441796602179295
1106	8	0.19087067277765613
1106	9	0.3761742596852926
1106	10	0.4329634363453039
1106	11	0.36470516345269743
1106	12	0.1586527504281321
1106	13	0.14228372911809117
1106	14	0.6031309237114968
1106	15	0.44402555279999234
1106	16	0.3206786052396211
1106	17	0.1071555083320277
1106	18	0.23826979033412954
1106	19	0.16847625748334139
1106	20	0.3343447288417494
1106	21	0.4886831092073436
1106	22	0.6322344094422903
1106	23	0.7464359539534158
1106	24	0.5502957105787942
1106	25	0.4557412774273999
1107	1	0.37456484765188947
1107	2	0.23102837854032643
1107	3	0.2814942895939794
1107	4	0.3708168917316712
1107	5	0.3579425272002794
1107	6	0.2333694004439124
1107	7	0.18400556010229252
1107	8	0.2830197722776191
1107	9	0.4630695449836111
1107	10	0.3751577900409373
1107	11	0.17598234904166077
1107	12	0.3371249203796296
1107	13	0.2816216847441666
1107	14	0.9481002322811207
1107	15	0.6232894917177987
1107	16	0.5974161130614277
1107	17	0.660505672438731
1107	18	0.6459223640396061
1107	19	0.4175779929760992
1107	20	0.6496892544787158
1107	21	0.5822466458724662
1107	22	0.5758936536551786
1107	23	0.7249574902538853
1107	24	0.740985641709969
1107	25	0.6322354606276193
1108	1	0.4952825705129946
1108	2	0.4989637738876027
1108	3	0.5296476884627026
1108	4	0.6517923903235712
1108	5	0.45840621646850366
1108	6	0.45527784280299954
1108	7	0.4585669383604767
1108	8	0.5208425676546398
1108	9	0.18637198169111002
1108	10	0.4259800528829487
1108	11	0.2806223601721243
1108	12	0.33151224124876677
1108	13	0.2983210711295001
1108	14	-1.067708285400478
1108	15	0.06670803065282627
1108	16	0.4243773051722286
1108	17	0.49690223350994245
1108	18	0.6505574816051052
1108	19	0.64100900570417
1108	20	0.6955994335736051
1108	21	0.7855285967737018
1108	22	0.8601003923819874
1108	23	1.1378730954487395
1108	24	0.974532516784933
1108	25	0.7536812212171498
1109	1	0.42152601838719406
1109	2	0.3193966995331081
1109	3	0.3780169371522746
1109	4	0.5159216856060382
1109	5	0.46790922559294046
1109	6	0.33328405518894044
1109	7	0.2667109880595646
1109	8	0.32095710852048853
1109	9	0.4044325524540682
1109	10	0.5046987482818963
1109	11	0.22033309595158102
1109	12	0.2673441185541421
1109	13	0.23081767373093243
1109	14	0.6462967621630966
1109	15	0.39963561752915666
1109	16	0.3344666082201826
1109	17	0.5111188904697599
1109	18	0.5220406887493496
1109	19	0.5084774584317913
1109	20	0.6141123436881415
1109	21	0.5948782376201365
1109	22	0.6813778142418201
1109	23	1.0964306570872608
1109	24	0.8053168305104208
1109	25	0.7445237760608734
1110	1	0.45821717976339116
1110	2	0.3786494854928186
1110	3	0.41226238027243767
1110	4	0.6587545818987692
1110	5	0.6106034207090474
1110	6	0.4253810419774027
1110	7	0.40551036644941263
1110	8	0.43393291384095534
1110	9	0.5626089645225179
1110	10	0.5511250008142738
1110	11	0.3707668390162908
1110	12	0.3020488769556806
1110	13	0.3256266345031733
1110	14	0.8325562797557282
1110	15	0.4961695185972843
1110	16	0.47359674414398967
1110	17	0.5573234737763855
1110	18	0.5975440657124338
1110	19	0.6046913398818603
1110	20	0.698952468567505
1110	21	0.6812861725312729
1110	22	0.8435284813209094
1110	23	1.0129992798938119
1110	24	0.8108070265888543
1110	25	0.8446831913832114
1111	1	0.230413684986015
1111	2	0.09368582307036277
1111	3	0.3274607168510606
1111	4	0.5454489119337254
1111	5	0.34643089683989103
1111	6	0.2525794654187621
1111	7	0.1848506439758495
1111	8	0.3276241819645094
1111	9	0.4167057006552426
1111	10	0.2994458355651588
1111	11	0.22200499492042525
1111	12	0.16852152605194703
1111	13	0.1486648758223268
1111	14	-1.8359444694353684
1111	15	-0.6030211378678345
1111	16	-0.07757843627715033
1111	17	0.08671431265324969
1111	18	0.1765240800447856
1111	19	0.2042044018656597
1111	20	0.30728730993443065
1111	21	0.3522482135160132
1111	22	0.16956243741179833
1111	23	-1.9822744824931746
1111	24	-0.4236180868755923
1111	25	0.016681577184044727
1112	1	-0.04923212721301695
1112	2	-0.18518845605595402
1112	3	0.0858976204931956
1112	4	0.25880238455975757
1112	5	-0.6052148725661155
1112	6	-0.7275021643745845
1112	7	-0.8616374051628455
1112	8	-0.6032407140218723
1112	9	-0.5956830899215646
1112	10	-0.7399912585478958
1112	11	-0.7330873610985265
1112	12	-0.5705093349912715
1112	13	-0.8644809037924759
1112	14	-0.42905027533011975
1112	15	-0.8719374730284031
1112	16	-0.8676130594705782
1112	17	-0.9612917351479595
1112	18	-0.9658440496119312
1112	19	-0.7677760257793054
1112	20	-0.802178996711433
1112	21	-1.6421585912462646
1112	22	-1.5345555731656333
1112	23	-1.3102080982045408
1112	24	-1.7254325740767058
1112	25	-1.9140785038597912
1113	1	0.10925657408596123
1113	2	-0.16951999736992818
1113	3	-0.03713729202367233
1113	4	0.25901380929219187
1113	5	0.12667256569901042
1113	6	-0.06585844094724536
1113	7	-0.02568898910753603
1113	8	0.0045786390560280665
1113	9	0.22977496254709706
1113	10	-0.17473787465029034
1113	11	-0.1472208857937929
1113	12	-0.03310388204401704
1113	13	-0.13896466852212086
1113	14	0.05342926664491898
1113	15	-0.04729354928057461
1113	16	0.0422347922809152
1113	17	0.011802998473108062
1113	18	0.05456786713060069
1113	19	-0.0501479693756437
1113	20	-0.7230254445794699
1113	21	-0.590392058681239
1113	22	-0.12260585958840409
1113	23	-0.25950975937495
1113	24	0.06287134002445072
1113	25	-1.141133154727271
1114	1	-1.1183326096588324
1114	2	-0.8768732770462506
1114	3	-0.6346780715093772
1114	4	-1.6709495445738796
1114	5	-0.9016998396754445
1114	6	-0.7041818416827041
1114	7	-0.5993594586029118
1114	8	-0.6014391482952348
1114	9	-0.6864355793084442
1114	10	-0.6989544482677843
1114	11	-0.7551507734833436
1114	12	-0.7661717208953363
1114	13	-0.8481906743066716
1114	14	-0.6522237248045002
1114	15	-1.5228156801851185
1114	16	-0.9756222948903073
1114	17	-1.0548064447694543
1114	18	-0.9392523663095815
1114	19	-1.0330612669441095
1114	20	-2.343695585750652
1114	21	-6.999820312305971
1114	22	-7.9957977762680095
1114	23	-8.124157605205337
1114	24	-6.214047414353107
1114	25	-7.46020464390212
1115	1	-1.0994900668930967
1115	2	-1.0553439041064898
1115	3	-0.7613334014821724
1115	4	-0.720789425320838
1115	5	-0.7886142287202489
1115	6	-0.9639711047000858
1115	7	-1.1345935761418886
1115	8	-0.8474669245882893
1115	9	-2.966015848885963
1115	10	-1.7872141880399965
1115	11	-1.2589224494480504
1115	12	-1.3009579283137214
1115	13	-1.5811367475851767
1115	14	-1.1978894776875961
1115	15	-2.1831319432964236
1115	16	-1.4711659227951088
1115	17	-3.226008705480126
1115	18	-2.924281910854134
1115	19	-2.2369318218724104
1115	20	-2.039353233139586
1115	21	-1.5146532933014285
1115	22	-0.8982393373414581
1115	23	-0.3827049056212033
1115	24	-1.308062091639698
1115	25	-1.0821248402347123
1116	1	-1.6830910019557734
1116	2	-0.9622580981677267
1116	3	-0.6075461221496106
1116	4	-0.2940442906353127
1116	5	-0.3664823087277327
1116	6	-0.4032251711871453
1116	7	-0.9391140701201763
1116	8	-1.7312184876251422
1116	9	-0.9671171233093645
1116	10	-2.535257283624432
1116	11	-1.2305474377079406
1116	12	-0.8382674692098036
1116	13	-0.9512192333804421
1116	14	-1.2720727775758292
1116	15	-1.1953644313510594
1116	16	-0.8137994674913074
1116	17	-0.853512917144478
1116	18	-1.292104613354745
1116	19	-0.8547544107224141
1116	20	-1.2441039014279056
1116	21	-0.6344821336624165
1116	22	-0.19763837109305626
1116	23	-0.04812878012328525
1116	24	-0.10037255492413522
1116	25	-0.2860860004565282
1201	1	0.30693118663814545
1201	2	0.40693078740781274
1201	3	0.3368360511724049
1201	4	0.4623685919233438
1201	5	0.3998764507716075
1201	6	0.39758477279521287
1201	7	0.42018299272445603
1201	8	0.502794149731514
1201	9	0.775365398552438
1201	10	0.6702612166119764
1201	11	0.48049848390681404
1201	12	0.5572636392809045
1201	13	0.5528216728142183
1201	14	1.1948867453916208
1201	15	0.9299535888038832
1201	16	0.9868456282415323
1201	17	0.8353824487007131
1201	18	0.8247466229656197
1201	19	0.7663300221974083
1201	20	0.9667836906699421
1201	21	0.9348115301636639
1201	22	0.9904040661980815
1201	23	0.8222937084592914
1201	24	0.7909471837347439
1201	25	0.8371320600364314
1202	1	-0.2715414879468163
1202	2	0.07998334866523749
1202	3	-0.049181296757019766
1202	4	0.01694184639605112
1202	5	0.10204193382458016
1202	6	0.057433052272955365
1202	7	0.1256386574359638
1202	8	0.2833006571298853
1202	9	0.3347456549378123
1202	10	0.5193120635961677
1202	11	0.24434454381792392
1202	12	0.10001106742341648
1202	13	0.27306630143072397
1202	14	0.5898817354307866
1202	15	0.6277884459555381
1202	16	0.5457329907854316
1202	17	0.46805307833353993
1202	18	0.3724246905460191
1202	19	0.27809246303313984
1202	20	0.5518035138566404
1202	21	0.64090521285015
1202	22	0.5915159265551821
1202	23	0.8096703708513147
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2211	6	0.05673977188600787
2211	7	0.017610615711653566
2211	8	0.1753723316560425
2211	9	0.308884750271595
2211	10	0.3500074392866068
2211	11	0.14247145609791545
2211	12	0.1364930149953878
2211	13	-0.07499200859341215
2211	14	0.36789095729060506
2211	15	0.1572880470905389
2211	16	-0.003666313472834304
2211	17	0.1559412143717509
2211	18	0.11525837393809013
2211	19	0.20775243061904547
2211	20	0.22724731428277067
2211	21	0.2714767005661187
2211	22	0.365529240567966
2211	23	0.3846491022586491
2211	24	0.4348115176068177
2211	25	0.4342117777511447
2212	1	-0.13011554014827453
2212	2	-0.22494271178358716
2212	3	-0.08748734368781186
2212	4	-0.44064549171183387
2212	5	-0.0388113750215382
2212	6	-0.4473719944598429
2212	7	-0.26250950841256326
2212	8	-0.22085545568425147
2212	9	-0.047667075227202815
2212	10	-0.4137627430208397
2212	11	-0.3148606913480805
2212	12	-0.5664126615058578
2212	13	-0.3884035458427988
2212	14	-0.3187061420330153
2212	15	-0.4650082510015352
2212	16	-0.2989465020834672
2212	17	-0.35895551039733675
2212	18	-0.26852880878149676
2212	19	-0.4153528264780988
2212	20	-0.4018388453817039
2212	21	-0.47100311712643617
2212	22	-0.4519739110235861
2212	23	0.10212331375468864
2212	24	-0.14793791268240142
2212	25	-0.8292623367728424
2213	1	-0.07414584538248903
2213	2	-1.244545464320474
2213	3	-0.31200430167467275
2213	4	0.14690708072885883
2213	5	0.13954821055519062
2213	6	-0.024750656348430766
2213	7	0.08032609384629552
2213	8	0.19524168386072915
2213	9	0.146147724323491
2213	10	-0.298349256412898
2213	11	-0.040418445916238
2213	12	-0.04919810276908265
2213	13	-0.31572644880390044
2213	14	0.07948481502967297
2213	15	-0.12532853792125564
2213	16	-0.0549614504715592
2213	17	0.0714057398662149
2213	18	0.02978160021280729
2213	19	0.09510840049747316
2213	20	0.20059607131416612
2213	21	-0.2855002546144334
2213	22	-0.8047325086608481
2213	23	-0.051495653684007436
2213	24	0.15573898086212523
2213	25	-0.6072791041214742
2214	1	-0.3433492152890203
2214	2	-0.5384623535272546
2214	3	-0.4864214952903865
2214	4	-0.7227989082444921
2214	5	-0.47435492041071825
2214	6	-0.4018822710583194
2214	7	-0.5657622479935043
2214	8	-0.25962429263406506
2214	9	-0.11792729439368088
2214	10	-2.2373074758352445
2214	11	-1.0200856606928212
2214	12	-0.6075287517143764
2214	13	-0.3397389517005607
2214	14	-0.9877975827258965
2214	15	-0.6832107636887912
2214	16	-0.8188337861913553
2214	17	-0.7275135047824293
2214	18	-0.6822654987292509
2214	19	-1.5161526975917425
2214	20	-2.599738176783731
2214	21	-2.3274878610990584
2214	22	-2.410648231312152
2214	23	-2.7882621220870547
2214	24	-1.8805783824015663
2214	25	-2.2155948635719582
2215	1	-0.3691829097343877
2215	2	-0.5580886646569176
2215	3	-1.0353421772298859
2215	4	-0.542910076804894
2215	5	-0.4258180449892066
2215	6	-0.42719752674656775
2215	7	-0.538866074981172
2215	8	-0.4667995910642091
2215	9	-0.4616710946837088
2215	10	-0.44071349011547056
2215	11	-0.7417662923733346
2215	12	-0.6944600211889593
2215	13	-0.5598217149512337
2215	14	-0.34740447891007165
2215	15	-0.9195359648628383
2215	16	-0.6953027773871057
2215	17	-0.5507258188469351
2215	18	-0.5655229543557212
2215	19	-2.4942856316509605
2215	20	-2.797832495805494
2215	21	-3.5872367698803416
2215	22	-2.6038103366199508
2215	23	-3.6931829513546823
2215	24	-2.7077389787121717
2215	25	-1.9811672096356858
2216	1	-1.7906994114689887
2216	2	-1.3357951026359807
2216	3	-1.0367338695659143
2216	4	-1.1067015153468063
2216	5	-2.0917506462765942
2216	6	-1.813003026301402
2216	7	-1.4227653350894442
2216	8	-5.710683664459829
2216	9	-4.672219433253247
2216	10	-2.4515016641281804
2216	11	-1.4866897754929553
2216	12	-1.509353683499512
2216	13	-2.2329550284995463
2216	14	-3.853514326735933
2216	15	-2.3275315029984753
2216	16	-3.4635474431054547
2216	17	-3.0925169503798315
2216	18	-3.0300519513572652
2216	19	-2.1799883445873007
2216	20	-1.2816491090867075
2216	21	-1.0721290608839595
2216	22	-0.9394163007817653
2216	23	-1.4143929352878786
2216	24	-0.837192973411149
2216	25	-0.5953012907065371
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	26.0
8	165.0
9	631.0
10	1406.0
11	2890.0
12	5045.0
13	7664.0
14	10630.0
15	14327.0
16	17699.0
17	19893.0
18	21089.0
19	22038.0
20	22663.0
21	24584.0
22	26024.0
23	27627.0
24	29341.0
25	31095.0
26	33005.0
27	37068.0
28	41790.0
29	48917.0
30	58142.0
31	71258.0
32	93862.0
33	131697.0
34	186986.0
35	289778.0
36	488064.0
37	1442826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01131047126704	19.232602842662068	11.369837600028685	24.38624908604221
2	13.481927808498554	22.290231209079522	36.90744217279049	27.320398809631435
3	20.833347622558687	27.662776360113423	27.43621679508783	24.067659222240064
4	13.209465399824664	18.518959606628847	33.378684581033035	34.89289041251345
5	12.35335032029029	39.55761998454276	34.45102646592004	13.638003229246914
6	33.26955567608681	33.57126825892607	17.61201613456275	15.547159930424373
7	25.306516461262586	33.38991699158632	22.447836485487787	18.855730061663305
8	26.760270119053743	36.28799815473713	20.85025473666821	16.101476989540913
9	26.445456917212635	17.378149227902064	20.08420903015298	36.09218482473232
10	16.335289346822822	31.46411755281579	31.875684568356466	20.324908532004915
11	30.572271412175933	27.052694378588853	19.827516880192555	22.54751732904266
12	22.94014822055538	30.158606467443345	28.68777803822215	18.213467273779123
13	28.492566897609805	22.58738605968738	23.270334389082304	25.649712653620515
14	21.241697847144383	25.489557397222868	25.703010157708178	27.565734597924568
15	29.44811321636902	26.159590643602964	21.30786575972879	23.084430380299224
16	20.44049172387008	29.108686585568844	29.255687559964617	21.195134130596465
17	24.90883355261907	29.88928486043172	26.699370944499666	18.50251064244954
18	25.200223679477535	26.74846220359196	27.82103896249628	20.23027515443422
19	23.034227422895643	30.453582161057064	28.182476434729946	18.32971398131735
20	23.61091370178641	27.75292498279422	26.818856788054703	21.817304527364673
21	20.358234396579128	29.3198968397744	29.179825044400264	21.14204371924621
22	22.08687270129554	28.84259158163345	23.4982733395516	25.57226237751941
23	19.39331718016461	29.790679515579633	27.129898866439373	23.686104437816383
24	21.447266831016403	32.94758211694474	24.746501074278747	20.85864997776011
25	22.552996730612122	26.601632975674068	22.994871762829575	27.85049853088423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10448.0
1	10448.0
2	11973.0
3	13498.0
4	13498.0
5	13498.0
6	15187.0
7	16876.0
8	16876.0
9	16876.0
10	17306.5
11	17737.0
12	17737.0
13	17737.0
14	21729.5
15	25722.0
16	25722.0
17	25722.0
18	35242.5
19	44763.0
20	44763.0
21	44763.0
22	60020.5
23	75278.0
24	75278.0
25	75278.0
26	96419.5
27	117561.0
28	117561.0
29	117561.0
30	141522.5
31	165484.0
32	165484.0
33	165484.0
34	188915.5
35	212347.0
36	212347.0
37	212347.0
38	234937.5
39	257528.0
40	257528.0
41	257528.0
42	292557.5
43	327587.0
44	327587.0
45	327587.0
46	509551.5
47	691516.0
48	691516.0
49	691516.0
50	604545.5
51	517575.0
52	517575.0
53	517575.0
54	396482.5
55	275390.0
56	275390.0
57	275390.0
58	225083.0
59	174776.0
60	174776.0
61	174776.0
62	150104.5
63	125433.0
64	125433.0
65	125433.0
66	100397.0
67	75361.0
68	75361.0
69	75361.0
70	56817.5
71	38274.0
72	38274.0
73	38274.0
74	27551.0
75	16828.0
76	16828.0
77	16828.0
78	11271.5
79	5715.0
80	5715.0
81	5715.0
82	3771.5
83	1828.0
84	1828.0
85	1828.0
86	1188.0
87	548.0
88	548.0
89	548.0
90	339.5
91	131.0
92	131.0
93	131.0
94	73.5
95	16.0
96	16.0
97	16.0
98	14.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03204256049981407
2	0.01517969548969791
3	0.02144482648236584
4	0.02110195861709545
5	0.02100844919929444
6	0.0066391686638719825
7	1.2467922373468512E-4
8	5.61056506806083E-4
9	8.104149542754531E-4
10	2.1818864153569893E-4
11	0.0010909432076784947
12	0.0012779620432805223
13	0.002618263698428387
14	9.03924372076467E-4
15	0.0023377354450253456
16	0.002836452339964086
17	0.002805282534030415
18	0.002213056221290661
19	4.987168949387405E-4
20	2.805282534030415E-4
21	0.08521824942265727
22	0.08175840096401976
23	0.0832233818429023
24	0.0010597734017448234
25	0.2284123378819431
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3208233.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.44562120505334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.6281079347123	45.764474211840565
2	9.2559488290655	9.893798700231834
3	2.347557147213712	3.7639995014159906
4	0.9073626872105209	1.9397824990501116
5	0.4660643111068426	1.245454831430522
6	0.29837283867049297	0.9568033028075793
7	0.18505785148578136	0.6923372282071061
8	0.13125293737478738	0.5611915818386779
9	0.10444914080033572	0.5024114292967223
>10	0.5837695091140985	5.892186499543742
>50	0.05483927318491495	2.020116385485486
>100	0.029230622015456478	2.8150870195613114
>500	0.0031947672181100025	1.1875090260157204
>1k	0.0027806307268580066	2.957674054319452
>5k	0.0011240847619213218	4.627984105949522
>10k+	8.874353383589384E-4	15.179189623005641
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	97335	3.0339130605538935	No Hit
CCCATGTACTCTGCGTTGATACCAC	93876	2.9260967018293247	No Hit
GAGTACATGGGAAGCAGTGGTATCA	45820	1.428200507880818	No Hit
CATGTACTCTGCGTTGATACCACTG	37890	1.1810239468268047	No Hit
GCGTTGATACCACTGCTTCCCATGT	30309	0.9447256480436428	No Hit
GCTTCCCATGTACTCTGCGTTGATA	25089	0.7820192610698786	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	24774	0.7722007722007722	No Hit
GTATCAACGCAGAGTACATGGGAAG	23079	0.7193679511431994	No Hit
ACTCTGCGTTGATACCACTGCTTCC	20808	0.6485813218678319	No Hit
TATCAACGCAGAGTACATGGGAAGC	19483	0.6072813290057175	No Hit
GGTATCAACGCAGAGTACATGGGAA	16058	0.5005247436828933	No Hit
CAGTGGTATCAACGCAGAGTACATG	12881	0.40149827023161966	No Hit
GTGGTATCAACGCAGAGTACATGGG	11698	0.36462438981208656	No Hit
GCAGTGGTATCAACGCAGAGTACAT	10631	0.3313662068808594	No Hit
ATACCACTGCTTCCCATGTACTCTG	10324	0.32179707645922223	No Hit
GTACTCTGCGTTGATACCACTGCTT	9617	0.2997600236641167	No Hit
GTACATGGTAAGCAGTGGTATCAAC	9612	0.29960417463444833	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	9117	0.284175120697281	No Hit
CATGGGAAGCAGTGGTATCAACGCA	9081	0.28305300768366887	No Hit
ACATGGGAAGCAGTGGTATCAACGC	8726	0.27198772657721554	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	8563	0.26690704821002714	No Hit
TACCACTGCTTCCCATGTACTCTGC	8134	0.25353520146448216	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7998	0.24929610785750286	No Hit
GTTGATACCACTGCTTCCCATGTAC	7856	0.24486999541492152	No Hit
CTTCCCATGTACTCTGCGTTGATAC	7791	0.24284395802923292	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	7604	0.2370152043196364	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	7427	0.23149814866937657	No Hit
GATACCACTGCTTCCCATGTACTCT	7414	0.23109294119223883	No Hit
CCACTGCTTCCCATGTACTCTGCGT	7174	0.22361218776815772	No Hit
ACCATGTACTCTGCGTTGATACCAC	7029	0.2190925659077754	No Hit
CCATGTACTCTGCGTTGATACCACT	6509	0.20288426682226632	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	5978	0.18633309987148688	No Hit
TTCCCATGTACTCTGCGTTGATACC	5686	0.17723151653885488	No Hit
TGATACCACTGCTTCCCATGTACTC	5048	0.1573451803531726	No Hit
GTACATGGGTAAGCAGTGGTATCAA	4884	0.1522333321800505	No Hit
CCCCATGTACTCTGCGTTGATACCA	4035	0.1257701669423636	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	3956	0.12330775227360356	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	3618	0.11277235786802267	No Hit
ATCAACGCAGAGTACATGGGAAGCA	3543	0.11043462242299733	No Hit
GCTTACCATGTACTCTGCGTTGATA	3477	0.10837741523137502	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	6.233961186734255E-5	0.0	0.0	3.116980593367127E-5	0.0
10	6.233961186734255E-5	0.0	0.0	6.233961186734255E-5	0.0
11	6.233961186734255E-5	0.0	3.116980593367127E-5	6.233961186734255E-5	0.0
12	6.233961186734255E-5	0.0	3.116980593367127E-5	9.350941780101383E-5	0.0
13	6.233961186734255E-5	0.0	3.116980593367127E-5	9.350941780101383E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	70	1.0902532E-4	12.215899	1
GGTATCG	110	3.7880454E-8	12.097978	17
GTATCGA	110	3.788955E-8	12.09779	18
CTAGACT	135	3.7289283E-10	11.961745	4
TATCGAC	120	9.740688E-9	11.898279	19
TTACGCT	65	8.034364E-4	11.691118	4
CTAGGAC	205	0.0	11.12134	3
CCGTATA	70	0.0014920603	10.857223	2
AGAAACG	80	3.7581212E-4	10.6920805	15
CTTAGAC	160	5.456968E-10	10.686913	3
CGCAAAA	220	0.0	10.363712	2
TAGACAG	300	0.0	10.131828	5
ATCGTCG	75	0.0026590175	10.128671	9
GTCTCGC	95	1.6428696E-4	10.001321	1
GCTCTAG	105	4.101952E-5	9.953695	1
CACACCG	125	2.5770714E-6	9.878532	5
ACTGATC	290	0.0	9.823064	8
AAGCGGT	175	2.5956979E-9	9.767237	10
CCGGTAC	90	0.0011147537	9.499478	3
GCTAGAC	130	4.2449374E-6	9.499477	3
>>END_MODULE