FastQCFastQC Report
Wed 25 May 2016
SRR1294720_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294720_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3208233
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1026173.198551975495546No Hit
GTACATGGGAAGCAGTGGTATCAAC973743.035128682985307No Hit
GAGTACATGGGAAGCAGTGGTATCA506981.5802468212252663No Hit
CATGTACTCTGCGTTGATACCACTG431401.3446654279785788No Hit
GCGTTGATACCACTGCTTCCCATGT332411.036115519041167No Hit
ACGCAGAGTACATGGGAAGCAGTGG309430.9644873050055904No Hit
GCTTCCCATGTACTCTGCGTTGATA286090.8917369779564015No Hit
GTATCAACGCAGAGTACATGGGAAG252560.7872246186608018No Hit
TATCAACGCAGAGTACATGGGAAGC235620.7344229674091626No Hit
ACTCTGCGTTGATACCACTGCTTCC235530.7341424391557595No Hit
GGTATCAACGCAGAGTACATGGGAA184860.5762050324898472No Hit
CAGTGGTATCAACGCAGAGTACATG158070.4927011223935419No Hit
GTGGTATCAACGCAGAGTACATGGG132330.412470041920272No Hit
GCAGTGGTATCAACGCAGAGTACAT125690.39177329078031425No Hit
CATGGGAAGCAGTGGTATCAACGCA120490.3755649916948052No Hit
ACATGGGAAGCAGTGGTATCAACGC117080.36493608787142334No Hit
ATACCACTGCTTCCCATGTACTCTG116630.3635334466044081No Hit
GGGAAGCAGTGGTATCAACGCAGAG104020.32422832132204865No Hit
CTGCTTCCCATGTACTCTGCGTTGA100480.31319421002152903No Hit
GTACTCTGCGTTGATACCACTGCTT95470.29757813724875964No Hit
AAGCAGTGGTATCAACGCAGAGTAC95320.2971105901597546No Hit
CTTCCCATGTACTCTGCGTTGATAC92260.28757262954405116No Hit
GTACATGGTAAGCAGTGGTATCAAC91090.28392576224981164No Hit
GCAGAGTACATGGGAAGCAGTGGTA89480.2789074234944906No Hit
TACCACTGCTTCCCATGTACTCTGC89170.27794115951054676No Hit
GTTGATACCACTGCTTCCCATGTAC84630.26379006761666No Hit
GATACCACTGCTTCCCATGTACTCT83620.26064191721735924No Hit
CCACTGCTTCCCATGTACTCTGCGT82830.25817950254859917No Hit
GTATCAACGCAGAGTACTTTTTTTT81180.2530364845695434No Hit
CCATGTACTCTGCGTTGATACCACT80260.25016886242364567No Hit
ATGGGAAGCAGTGGTATCAACGCAG76750.23922826054092705No Hit
ACCATGTACTCTGCGTTGATACCAC76490.23841784558665158No Hit
TATCAACGCAGAGTACTTTTTTTTT65390.20381936100027648No Hit
GGTATCAACGCAGAGTACTTTTTTT65390.20381936100027648No Hit
TTCCCATGTACTCTGCGTTGATACC61330.19116441979120594No Hit
TGATACCACTGCTTCCCATGTACTC56190.1751431395412989No Hit
GTACATGGGTAAGCAGTGGTATCAA53380.16638442407393728No Hit
GGTAAGCAGTGGTATCAACGCAGAG48770.1520151435385148No Hit
CCCCATGTACTCTGCGTTGATACCA46580.1451889560390408No Hit
GTACTTTTTTTTTTTTTTTTTTTTT45850.1429135602058828No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44930.14004593805998505No Hit
ATCAACGCAGAGTACATGGGAAGCA43570.13580684445300575No Hit
GCTTACCATGTACTCTGCGTTGATA39050.12171809217098634No Hit
GAGTACATGGTAAGCAGTGGTATCA35750.1114320562128748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA33830.10544745347360993No Hit
GGAAGCAGTGGTATCAACGCAGAGT33100.10317205764045193No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG657.9367077E-411.7081735
GTCCTAG1950.011.2236621
CTAATAC1950.011.2203323
CCGTTTT600.00590127511.0774429
GTATTAG1900.011.0181951
TGTCCGC700.001498784910.85137210
GTAAGGC1158.6729597E-710.7537023
ACGCAAA1605.293259E-1010.7051771
TTAGACC1451.4151738E-810.4969824
CCGTCAC1106.0901802E-610.35796613
CTTCTAG2850.010.3504251
CGTATAT856.5152894E-410.0724723
TATACTG6650.010.0135695
AGTACCG1151.0108095E-59.9264945
TAGGCTC1252.5279896E-69.8934065
TAATACA5600.09.8526354
TAGACTG2450.09.707035
TCTTGTA3350.09.6550242
GGACCGC1401.0644944E-69.4967266
TCACCCG1304.2716238E-69.49480218
Produced by FastQC (version 0.11.5)