FastQCFastQC Report
Wed 25 May 2016
SRR1294719_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294719_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2446434
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC57960.2369162626091691No Hit
CCCATGTACTCTGCGTTGATACCAC52690.2153747045699986No Hit
GTATCAACGCAGAGTACTTTTTTTT37400.15287557318120987No Hit
GGTATCAACGCAGAGTACTTTTTTT30260.1236902364829789No Hit
GAGTACATGGGAAGCAGTGGTATCA27180.1111004833974675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG701.0932703E-412.2120385
GGCCCGT550.003078724312.0859676
CCTAGAC2300.011.5647443
GGACCGT700.001497029710.8527046
TGGCCCG803.778761E-410.6855345
CTAGACT2150.010.6037854
CCAGTAC3250.010.5226463
GTCTAGT1909.094947E-1210.5010741
CTAGACA2900.010.1543424
CTAAGAC2054.1836756E-119.7313093
GTCCAGG4500.09.7121051
GGACTGT3250.09.6422116
GGGTAGC1106.8313486E-59.5009721
GTCCTAA2303.6379788E-129.5009711
GTCTCGC1502.6456473E-79.5009711
GGGCCGT800.0045322739.4961176
TAAGACA4000.09.2617434
TAAGACT3300.09.2113684
AGGACCC1651.0691656E-79.2104265
GTAAGAC3000.09.1829583
Produced by FastQC (version 0.11.5)