##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294719_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2446434
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.160549599948332	33.0	33.0	33.0	27.0	33.0
2	31.187635554443734	33.0	33.0	33.0	27.0	33.0
3	31.146064026252088	33.0	33.0	33.0	27.0	33.0
4	34.677572744656096	37.0	37.0	37.0	27.0	37.0
5	34.85152838784942	37.0	37.0	37.0	33.0	37.0
6	35.029557715433974	37.0	37.0	37.0	33.0	37.0
7	35.01912702325099	37.0	37.0	37.0	33.0	37.0
8	34.908182276734216	37.0	37.0	37.0	33.0	37.0
9	34.63083328632614	37.0	37.0	37.0	27.0	37.0
10	34.73279230095723	37.0	37.0	37.0	27.0	37.0
11	34.9349898668838	37.0	37.0	37.0	33.0	37.0
12	34.87174229919957	37.0	37.0	37.0	33.0	37.0
13	34.795266906852994	37.0	37.0	37.0	33.0	37.0
14	36.00885247670691	40.0	37.0	40.0	27.0	40.0
15	36.32148016255497	40.0	37.0	40.0	27.0	40.0
16	36.34889598493154	40.0	37.0	40.0	27.0	40.0
17	36.29540179706463	40.0	37.0	40.0	27.0	40.0
18	36.313622603348385	40.0	37.0	40.0	27.0	40.0
19	36.41416608827379	40.0	37.0	40.0	27.0	40.0
20	36.301676235696526	40.0	37.0	40.0	27.0	40.0
21	36.25677251051939	40.0	37.0	40.0	27.0	40.0
22	36.11782537358457	40.0	37.0	40.0	27.0	40.0
23	35.75920830073486	37.0	37.0	40.0	27.0	40.0
24	36.06215127814607	40.0	37.0	40.0	27.0	40.0
25	34.97426907899416	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5379711458254945
1101	2	0.6607629665269101
1101	3	0.48818505149705516
1101	4	0.6346290571867783
1101	5	0.5318461670514765
1101	6	0.41229028126819145
1101	7	0.46060446901189067
1101	8	0.6561293198507983
1101	9	0.8513041495943696
1101	10	0.840828827490192
1101	11	0.5244538070966058
1101	12	0.5412141934698624
1101	13	0.6512072301796508
1101	14	1.2370900415566126
1101	15	0.998507269569167
1101	16	0.9278793793059492
1101	17	0.8974182570232117
1101	18	0.8472416163305851
1101	19	0.9363932290434676
1101	20	1.0312778909405296
1101	21	1.0530701026867817
1101	22	1.096054695161385
1101	23	1.3082234069840482
1101	24	1.133979818940574
1101	25	1.0021697177072824
1102	1	0.2793252290033692
1102	2	0.4954304077332097
1102	3	0.3669969499744745
1102	4	0.4703181004489636
1102	5	0.37506737456694594
1102	6	0.3926635563687171
1102	7	0.41194804221166237
1102	8	0.40623170709677225
1102	9	0.7707362809706098
1102	10	0.7480753430775451
1102	11	0.5574442085462223
1102	12	0.6424503845352518
1102	13	0.6448757510625498
1102	14	1.2444783339046381
1102	15	0.9778046919119916
1102	16	0.6095140350047856
1102	17	0.8503710287202466
1102	18	0.8111864087133114
1102	19	0.761720388259242
1102	20	0.9549117829870539
1102	21	1.022156284268263
1102	22	1.0699995409185163
1102	23	1.2394717554644288
1102	24	1.111860827952647
1102	25	0.9470866141560705
1103	1	0.42889735279609553
1103	2	0.6268694758076272
1103	3	0.3877469636622344
1103	4	0.3434034661261052
1103	5	0.4191584790338112
1103	6	0.43792551490771103
1103	7	0.46773069569864134
1103	8	0.6230156060125083
1103	9	0.7956887514066224
1103	10	0.7635200914147191
1103	11	0.5700799769581408
1103	12	0.5836915873966575
1103	13	0.5951910729829137
1103	14	1.3236496706669385
1103	15	1.1133802165622342
1103	16	0.782844332907068
1103	17	0.7720182828928088
1103	18	0.8503378053996755
1103	19	1.0151976297635485
1103	20	1.0720867899567992
1103	21	1.0612542672274685
1103	22	0.9484841769594254
1103	23	1.3873243348361228
1103	24	1.1836234683623061
1103	25	1.086428475225631
1104	1	0.2741813448931474
1104	2	0.5349501211080749
1104	3	0.36675707405943925
1104	4	0.337737243579042
1104	5	0.3111865731937371
1104	6	0.28814638327040853
1104	7	0.3156498248462398
1104	8	0.45839041050333407
1104	9	0.5719975998221543
1104	10	0.5647516605537888
1104	11	0.3909641467852083
1104	12	0.39942472985305244
1104	13	0.41017499470282814
1104	14	0.7146820392213016
1104	15	0.6493391143403571
1104	16	0.5797042922584126
1104	17	0.601468805051752
1104	18	0.6635863328412199
1104	19	0.6976757863508354
1104	20	0.7615074480508426
1104	21	0.8342705809197071
1104	22	0.8937862871643532
1104	23	1.211443881069961
1104	24	0.9122276160532365
1104	25	0.888857595831837
1105	1	0.3574398050978118
1105	2	0.4108647740011442
1105	3	0.3730942928416283
1105	4	0.4745354208045782
1105	5	0.3590867146190604
1105	6	0.21206182420507247
1105	7	0.3481753543652246
1105	8	0.3557051648995895
1105	9	-1.6026342217011091
1105	10	-0.08982777578233225
1105	11	0.16146095255528792
1105	12	0.3222908762776697
1105	13	0.3408459955982437
1105	14	-1.1736496222769048
1105	15	-0.06513576576320901
1105	16	0.17954955955777763
1105	17	0.2895378675973532
1105	18	0.35347973002549793
1105	19	0.4753259968368724
1105	20	0.5441798940970699
1105	21	0.6263040800293354
1105	22	0.6217889596532302
1105	23	0.9306376802594656
1105	24	0.7060436921628792
1105	25	0.5584050806912515
1106	1	0.059853661740525865
1106	2	0.15721718702022258
1106	3	0.19978156355874788
1106	4	-0.9420600530048233
1106	5	-0.11032967146399386
1106	6	-0.08477051242825695
1106	7	0.06888577532439655
1106	8	0.2672961996249512
1106	9	0.4211065022883673
1106	10	0.4420959389772037
1106	11	0.29494337210736177
1106	12	0.15907078146553033
1106	13	0.24867218451895923
1106	14	0.7001756955782668
1106	15	0.4550704422539411
1106	16	0.27835686980787244
1106	17	0.1916979426429961
1106	18	0.3629609434868115
1106	19	0.28683662551167544
1106	20	0.42695038507651617
1106	21	0.6505682809104556
1106	22	0.6491395802874322
1106	23	0.8729086486498332
1106	24	0.5804846188426112
1106	25	0.40392914387700785
1107	1	0.22819250971434002
1107	2	0.24996179609528468
1107	3	0.1007727433683705
1107	4	0.29511153479116103
1107	5	0.26846871910193215
1107	6	0.056758449946045175
1107	7	0.1716707496271468
1107	8	0.14426715800801304
1107	9	0.33741576850157173
1107	10	0.3549201464700005
1107	11	0.06397049896349927
1107	12	0.11296207997738605
1107	13	0.142537187413744
1107	14	0.613586945444851
1107	15	0.5553050974837959
1107	16	0.48637040250920904
1107	17	0.38684910201125433
1107	18	0.4432175602467865
1107	19	0.4657365760254635
1107	20	0.6400637473253283
1107	21	0.6565244160692814
1107	22	0.42679849411349124
1107	23	0.7358439575505642
1107	24	0.664625741888635
1107	25	0.5587587398689422
1108	1	0.5035339729511499
1108	2	0.4788952488622904
1108	3	0.4509698399367643
1108	4	0.7584851941687063
1108	5	0.5849147410228497
1108	6	0.41117016745204893
1108	7	0.35897714233716727
1108	8	0.45802072658344883
1108	9	0.31857496398522045
1108	10	0.48737931447518434
1108	11	0.37068743637973256
1108	12	0.34855137060153396
1108	13	0.3824145927532143
1108	14	-0.8774193687349339
1108	15	0.03943241083033655
1108	16	0.39400137793253265
1108	17	0.5133904705860743
1108	18	0.5491596369261913
1108	19	0.5398938927667984
1108	20	0.605772321618943
1108	21	0.8604731688664629
1108	22	0.7756319752304961
1108	23	1.065208291682275
1108	24	0.9293369209793951
1108	25	0.8291559238756605
1109	1	0.4134705573815083
1109	2	0.26141209803430243
1109	3	0.40296953897183485
1109	4	0.5379609641446876
1109	5	0.4290402137381406
1109	6	0.36451584098252
1109	7	0.15109473200264034
1109	8	0.2511480182181316
1109	9	0.4080661107929089
1109	10	0.4225930073999322
1109	11	0.2144099671220019
1109	12	0.2888596297146506
1109	13	0.29619460276315124
1109	14	0.7635993349701948
1109	15	0.3339610874343393
1109	16	0.29610416630050906
1109	17	0.4159405565182297
1109	18	0.4049428746897803
1109	19	0.42372605529167373
1109	20	0.5865773715166043
1109	21	0.4142996651686559
1109	22	0.6031627423298289
1109	23	0.9612526641448795
1109	24	0.7901967649987398
1109	25	0.6217996579306941
1110	1	0.32423092835492895
1110	2	0.24941809242023538
1110	3	0.27339717240925765
1110	4	0.5390349560543868
1110	5	0.37692644386818586
1110	6	0.26367406644137503
1110	7	0.27926076366359354
1110	8	0.3723064640311904
1110	9	0.4743813201045768
1110	10	0.44460554719151446
1110	11	0.2824803607629178
1110	12	0.21987688645508285
1110	13	0.2147437300088484
1110	14	0.6504185300492864
1110	15	0.5754987545167083
1110	16	0.37464789339195903
1110	17	0.4840711382600418
1110	18	0.5539400282019145
1110	19	0.49555911107979966
1110	20	0.4424777982549344
1110	21	0.521549358826654
1110	22	0.6264627170065395
1110	23	0.8789208442593477
1110	24	0.6573816219141122
1110	25	0.5872267270627347
1111	1	0.08031704750071356
1111	2	0.06839902057648573
1111	3	0.16561570405042403
1111	4	0.4391416135849724
1111	5	0.24442410680576643
1111	6	0.23501024799176662
1111	7	0.2198974137789591
1111	8	0.26147312873339246
1111	9	0.28177175450613134
1111	10	0.18575398058045067
1111	11	0.1456344533920486
1111	12	0.14073291267104082
1111	13	0.07753924169652038
1111	14	-1.6195858467860162
1111	15	-0.499709472571638
1111	16	-0.018800395385923707
1111	17	0.12393892168763188
1111	18	0.16201695258848048
1111	19	0.17082491018489065
1111	20	0.3094857847352017
1111	21	0.3995497239558219
1111	22	0.09315738389427963
1111	23	-1.9084090949966779
1111	24	-0.41091337940469685
1111	25	-0.040703637513765045
1112	1	-0.1271899123428888
1112	2	-0.1941948998936276
1112	3	0.07670893103074405
1112	4	0.16006474175738816
1112	5	-0.8122025776989759
1112	6	-0.9156121274352813
1112	7	-0.8743128099568906
1112	8	-0.7398796944647046
1112	9	-0.5477648469410212
1112	10	-0.795464995633651
1112	11	-0.772461216729404
1112	12	-0.7960328689230423
1112	13	-0.8521154871209546
1112	14	-0.5613108287675601
1112	15	-0.9228671960215706
1112	16	-0.8592428625977107
1112	17	-0.8165391602738836
1112	18	-0.97786112829845
1112	19	-0.8806437760837014
1112	20	-1.0086932423220247
1112	21	-1.842281718495272
1112	22	-1.578376376048496
1112	23	-1.394507061655439
1112	24	-1.608363010247075
1112	25	-2.012754502130896
1113	1	0.1019004486662709
1113	2	-0.1227958546315513
1113	3	-0.08723666340361191
1113	4	0.045152231227945094
1113	5	0.01548308051263092
1113	6	-0.0666501404903812
1113	7	-0.07849272726825518
1113	8	0.024989520167437718
1113	9	0.12068923213180938
1113	10	-0.14747293411122087
1113	11	-0.1975952982711604
1113	12	-0.05224943497572809
1113	13	-0.2191845328722053
1113	14	-0.14430730951926307
1113	15	-0.17787759804977554
1113	16	0.013288432486177726
1113	17	-0.13465528547931882
1113	18	0.009898976830804429
1113	19	-0.12009582751016268
1113	20	-0.6101477847032157
1113	21	-0.5398398350097651
1113	22	-0.09960954974661718
1113	23	-0.2724320958529205
1113	24	-0.019679028807068732
1113	25	-1.162628737857787
1114	1	-0.9901926845974991
1114	2	-0.7679689612057494
1114	3	-0.5914879537646485
1114	4	-1.533138095982757
1114	5	-0.7391644424996429
1114	6	-0.6307363933488261
1114	7	-0.5702594111933692
1114	8	-0.5522426242166887
1114	9	-0.41497436682234934
1114	10	-0.5408244346266287
1114	11	-0.5772517365972689
1114	12	-0.5343967644797729
1114	13	-0.6080817969128276
1114	14	-0.6179857559705653
1114	15	-1.296046013173644
1114	16	-0.8112367185800977
1114	17	-0.8101439428564916
1114	18	-0.792540329042815
1114	19	-0.8449974296823157
1114	20	-2.048855524690481
1114	21	-6.948156837852913
1114	22	-8.070674969971119
1114	23	-8.40709875052693
1114	24	-6.078387716382927
1114	25	-7.616317878885191
1115	1	-1.1482213593477901
1115	2	-1.1108381582936104
1115	3	-0.9350741051424869
1115	4	-0.7712756418512683
1115	5	-0.7348652510626508
1115	6	-0.8969870246278404
1115	7	-1.1328649609492416
1115	8	-0.8376100724771547
1115	9	-2.789965282930396
1115	10	-1.6616703241108794
1115	11	-1.1966435381147207
1115	12	-1.2321285570498688
1115	13	-1.5185041365901384
1115	14	-1.4879986992890437
1115	15	-2.3005178539956006
1115	16	-1.519001752967732
1115	17	-3.1859239141719797
1115	18	-3.0143992871034584
1115	19	-2.265054523725169
1115	20	-1.9630304066695814
1115	21	-1.626268184098052
1115	22	-1.0858863628284254
1115	23	-0.45035809721025544
1115	24	-1.5147104099312827
1115	25	-1.1588586556604312
1116	1	-1.6111685402894302
1116	2	-1.0885673184542028
1116	3	-0.9426928870552373
1116	4	-0.589121576035339
1116	5	-0.7333118428957732
1116	6	-0.7055225711648134
1116	7	-1.061775911538838
1116	8	-1.9495478951261163
1116	9	-1.2069802401394583
1116	10	-2.6547251985416054
1116	11	-1.2815009989106514
1116	12	-1.0324698771293868
1116	13	-0.9796067143025766
1116	14	-1.57973410177339
1116	15	-1.5039361698534464
1116	16	-1.202827464089637
1116	17	-1.0702839026721804
1116	18	-1.5235936446181668
1116	19	-1.1549947988778868
1116	20	-1.3501099791931495
1116	21	-0.8794028963475427
1116	22	-0.5231452838579003
1116	23	-0.44937221432870444
1116	24	-0.37681260589646826
1116	25	-0.519370281360402
1201	1	0.337429163744428
1201	2	0.4054187559197473
1201	3	0.41250375913292814
1201	4	0.45581795257474766
1201	5	0.45455402308275694
1201	6	0.35205648817731827
1201	7	0.3362825827332401
1201	8	0.3786693498067706
1201	9	0.5305253069538693
1201	10	0.6389316944772219
1201	11	0.4160580955084825
1201	12	0.544795020679544
1201	13	0.4661886120233518
1201	14	1.1564198458657415
1201	15	0.8295398579387623
1201	16	0.8591320691149633
1201	17	0.7077142724379115
1201	18	0.7318697233462501
1201	19	0.6481062223408429
1201	20	0.8392486430426445
1201	21	0.8854713864513428
1201	22	0.8797014705966149
1201	23	0.7209855951022348
1201	24	0.7411400484511148
1201	25	0.6968625664734844
1202	1	-0.30023094888111856
1202	2	0.032872183484037976
1202	3	-0.15066815639223208
1202	4	-0.05796962347682921
1202	5	0.07326124218669605
1202	6	-0.0023182383723607813
1202	7	0.07437205369290467
1202	8	0.16420330184166687
1202	9	0.1744637571633234
1202	10	0.36673246959580297
1202	11	0.11808764444374731
1202	12	0.22627837937734085
1202	13	0.2034962572565462
1202	14	0.4161584898042676
1202	15	0.38838753334164977
1202	16	0.3309786009576712
1202	17	0.25014321853947763
1202	18	0.21237142701516376
1202	19	0.13174479943357653
1202	20	0.27797558052591853
1202	21	0.2413191946743467
1202	22	0.27932039280223364
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2211	6	0.08719454020933881
2211	7	0.026861989748539372
2211	8	0.05950740736015803
2211	9	0.3560067068588353
2211	10	0.2505897903212002
2211	11	0.0771173262532372
2211	12	0.1149589701320437
2211	13	0.0052294867830866565
2211	14	0.27672555591853154
2211	15	0.15509605478363397
2211	16	0.0993469974418133
2211	17	0.24103284130861624
2211	18	0.18244812128283883
2211	19	0.15354484383595945
2211	20	0.3136124678698451
2211	21	0.28072527582422424
2211	22	0.4307246111378902
2211	23	0.4465251598275586
2211	24	0.4051564102137206
2211	25	0.5721776315835427
2212	1	-0.3649511148769946
2212	2	-0.39322416181957465
2212	3	-0.23359390175969352
2212	4	-0.7237180029133938
2212	5	-0.43678915068657886
2212	6	-0.6879402480337546
2212	7	-0.5253043957753079
2212	8	-0.37210378402001254
2212	9	-0.28639204294336906
2212	10	-0.6686631833975483
2212	11	-0.5605673256243904
2212	12	-0.7126548047971895
2212	13	-0.6703967756446829
2212	14	-0.42993302983141035
2212	15	-0.7125141209659631
2212	16	-0.5975798409581259
2212	17	-0.6366850305833367
2212	18	-0.6311622075353185
2212	19	-0.6597890824656645
2212	20	-0.6726695381970487
2212	21	-0.5465086718863148
2212	22	-0.5834458658671835
2212	23	-0.11699389669093563
2212	24	-0.36122657973236727
2212	25	-1.0487369634001524
2213	1	-0.18177527871741717
2213	2	-1.2623252591503906
2213	3	-0.4190341197420153
2213	4	-0.09626140779970171
2213	5	-0.07006524773790801
2213	6	-0.030337291328372373
2213	7	-0.0015342305303178705
2213	8	0.15826718636110115
2213	9	0.20509924762215093
2213	10	-0.40923012240660483
2213	11	-0.14631122282064268
2213	12	0.047397963023385614
2213	13	-0.4656140312911532
2213	14	-0.0657138706000353
2213	15	-0.13241034357461245
2213	16	-0.1817143806066568
2213	17	0.046557629231912756
2213	18	0.04560008217342926
2213	19	0.030799351909436723
2213	20	0.13098706240482016
2213	21	-0.5163765389057176
2213	22	-0.8989706526158727
2213	23	-0.16371277897255254
2213	24	-0.07776291909693356
2213	25	-0.5785896058056821
2214	1	-0.1497688368109742
2214	2	-0.41637148753114417
2214	3	-0.34569303805303875
2214	4	-0.4958806496653736
2214	5	-0.22270229460676916
2214	6	-0.16920612406001823
2214	7	-0.4410015050201608
2214	8	-0.10573313098560533
2214	9	-0.05703370443185918
2214	10	-1.7367047028198286
2214	11	-0.9709084878479928
2214	12	-0.4842709795055882
2214	13	-0.2260635401565949
2214	14	-0.6938809964928652
2214	15	-0.4555846445142606
2214	16	-0.7063404848052457
2214	17	-0.5966826543416914
2214	18	-0.41592255657599253
2214	19	-1.308442884388775
2214	20	-2.19068748581045
2214	21	-1.7746785718935598
2214	22	-2.0069781554331954
2214	23	-2.460556569111411
2214	24	-1.5165106041453882
2214	25	-2.049004322353696
2215	1	-0.14804846174361685
2215	2	-0.25341720390586886
2215	3	-0.6250585014502441
2215	4	-0.18758866445239164
2215	5	-0.08772703349626454
2215	6	-0.06356908307581222
2215	7	-0.12079659563278256
2215	8	-0.10789692986061539
2215	9	-0.17831714742143845
2215	10	-0.1757769186491842
2215	11	-0.334317792416428
2215	12	-0.29932832700742296
2215	13	-0.19613233316472645
2215	14	-0.05622962971379053
2215	15	-0.4761171063295251
2215	16	-0.21569314136574036
2215	17	-0.21335406983539684
2215	18	-0.13619924823315444
2215	19	-2.00757313255081
2215	20	-2.3420045105053546
2215	21	-3.2930301390564694
2215	22	-2.428267230365016
2215	23	-3.310858077972057
2215	24	-1.948481307381691
2215	25	-1.1347694050449348
2216	1	-1.627815197828891
2216	2	-1.3140834323013308
2216	3	-0.9078526787663677
2216	4	-0.9565253911535123
2216	5	-1.8113690699191594
2216	6	-1.6041842000624271
2216	7	-1.2186397360316619
2216	8	-5.103191470666445
2216	9	-4.1322396294025445
2216	10	-2.0285997321600746
2216	11	-1.158855603363186
2216	12	-1.2697650636280855
2216	13	-1.9360303087376707
2216	14	-3.515604117600958
2216	15	-2.1091369462092544
2216	16	-3.1291425051432498
2216	17	-2.7454125032178567
2216	18	-2.6865208992564007
2216	19	-1.8144212922329928
2216	20	-1.1594005300280799
2216	21	-0.8904186795236768
2216	22	-0.7907012552845103
2216	23	-1.1170679904926502
2216	24	-0.8620442022436166
2216	25	-0.557208786511822
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	28.0
8	153.0
9	457.0
10	954.0
11	2028.0
12	3590.0
13	5659.0
14	7321.0
15	9783.0
16	10772.0
17	12026.0
18	12417.0
19	13067.0
20	13402.0
21	13913.0
22	15361.0
23	16682.0
24	17443.0
25	18985.0
26	20491.0
27	23268.0
28	26590.0
29	31377.0
30	38060.0
31	46415.0
32	62869.0
33	89817.0
34	130778.0
35	207230.0
36	364248.0
37	1231246.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.32634990313588	20.995144936365712	13.250758271476318	23.427746889022096
2	15.414276463393097	20.621185758858196	40.47568043118504	23.48885734656367
3	18.691993420963872	25.186849613912955	29.390081812759128	26.73107515236405
4	12.13019674732995	16.620637802650855	38.39269251069839	32.85647293932081
5	13.03770826383029	37.97830360238371	35.39364889351152	13.59033924027448
6	31.690761221699205	38.26400655693475	16.9914543191355	13.053777902230543
7	26.495677584466854	33.008179664025135	22.545845318469787	17.950297433038223
8	23.917757544171597	37.938542975975146	20.519819736963118	17.623879742890136
9	23.77111767855756	17.285941440723345	21.991530446654487	36.951410434064606
10	14.25252889006793	30.8795799918984	33.857185309690166	21.01070580834351
11	32.74824375021971	25.665690813380937	22.618077293793426	18.96798814260593
12	20.46260188523451	27.60695394828277	31.568480775676715	20.361963390806007
13	26.611507141838135	23.80717629750989	26.564498505738527	23.016818054913447
14	19.984630571673247	23.436293515204902	28.19419436195412	28.38488155116773
15	22.814994248640236	31.055053634393374	23.857433315701826	22.272518801264564
16	21.587349082394798	31.334083833666888	25.803190689217647	21.275376394720663
17	19.954912881303972	30.490133360584537	28.42732614582801	21.12762761228348
18	20.228378723621685	29.98125798375147	29.3662255377855	20.424137754841347
19	20.777879831705036	29.03149460497159	29.536436726496973	20.6541888368264
20	21.890776860747305	28.73174426499424	28.826167837696097	20.55131103656236
21	22.008988804495548	28.894023582315963	28.30712485077979	20.7898627624087
22	20.93812233169533	28.680603623903426	29.283810253162695	21.097463791238553
23	21.090804807893317	28.929997868595	28.895592655366535	21.08360466814515
24	21.070627976827954	28.47519371352798	29.031644917666643	21.42253339197742
25	21.501699913191562	28.456575503470493	28.25903407896823	21.78269050436971
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1984.0
1	1984.0
2	2327.0
3	2670.0
4	2670.0
5	2670.0
6	3993.5
7	5317.0
8	5317.0
9	5317.0
10	8183.5
11	11050.0
12	11050.0
13	11050.0
14	18028.5
15	25007.0
16	25007.0
17	25007.0
18	38597.0
19	52187.0
20	52187.0
21	52187.0
22	72631.5
23	93076.0
24	93076.0
25	93076.0
26	119756.5
27	146437.0
28	146437.0
29	146437.0
30	173767.0
31	201097.0
32	201097.0
33	201097.0
34	223908.5
35	246720.0
36	246720.0
37	246720.0
38	261692.0
39	276664.0
40	276664.0
41	276664.0
42	277277.5
43	277891.0
44	277891.0
45	277891.0
46	280749.5
47	283608.0
48	283608.0
49	283608.0
50	269175.5
51	254743.0
52	254743.0
53	254743.0
54	232178.5
55	209614.0
56	209614.0
57	209614.0
58	183116.0
59	156618.0
60	156618.0
61	156618.0
62	130018.5
63	103419.0
64	103419.0
65	103419.0
66	79792.0
67	56165.0
68	56165.0
69	56165.0
70	41366.0
71	26567.0
72	26567.0
73	26567.0
74	18473.0
75	10379.0
76	10379.0
77	10379.0
78	7001.0
79	3623.0
80	3623.0
81	3623.0
82	2400.0
83	1177.0
84	1177.0
85	1177.0
86	751.0
87	325.0
88	325.0
89	325.0
90	197.0
91	69.0
92	69.0
93	69.0
94	43.5
95	18.0
96	18.0
97	18.0
98	13.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030248108062592326
2	0.013816027736697576
3	0.019743021884097423
4	0.020642289961633954
5	0.020356159209690514
6	0.006908013868348788
7	4.087582170620585E-5
8	3.6788239535585264E-4
9	9.810197209489404E-4
10	2.0437910853102922E-4
11	0.0011445230077737638
12	0.002207294372135116
13	0.0030656866279654385
14	7.766406124179112E-4
15	0.0022890460155475276
16	0.002697804232609586
17	0.0024116734806661454
18	0.0024116734806661454
19	6.131373255930878E-4
20	4.496340387682643E-4
21	0.08420419271478405
22	0.08166989176899928
23	0.08326404881554132
24	0.0015532812248358224
25	0.22183308439957913
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2446434.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.65454457579622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.33862021931974	72.94904347038573
2	8.1557540733407	13.319087690616598
3	1.4125342356123798	3.46019519119948
4	0.4341537229037182	1.4180249807835819
5	0.18969262546726884	0.7744632470958462
6	0.11252802894965047	0.5513054973537433
7	0.07095185280359481	0.4055478859240526
8	0.04653237811782454	0.30396641145917674
9	0.030905592479886074	0.22712238709112254
>10	0.1859617686741501	2.678881333684062
>50	0.014012278576235502	0.794026449480963
>100	0.006646956488344776	1.0138392362446478
>500	7.527649705569494E-4	0.3821178659088351
>1k	8.531336332802979E-4	1.2689591370930662
>5k	1.0036866273885858E-4	0.4534192156791028
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	5796	0.2369162626091691	No Hit
CCCATGTACTCTGCGTTGATACCAC	5269	0.2153747045699986	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3740	0.15287557318120987	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3026	0.1236902364829789	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2718	0.1111004833974675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.087582170620585E-5	0.0
2	0.0	0.0	0.0	4.087582170620585E-5	0.0
3	0.0	0.0	0.0	8.17516434124117E-5	0.0
4	0.0	0.0	0.0	8.17516434124117E-5	0.0
5	0.0	0.0	0.0	8.17516434124117E-5	0.0
6	0.0	0.0	0.0	8.17516434124117E-5	0.0
7	0.0	0.0	0.0	8.17516434124117E-5	0.0
8	0.0	0.0	0.0	1.2262746511861755E-4	0.0
9	0.0	0.0	0.0	1.2262746511861755E-4	0.0
10	0.0	0.0	0.0	2.0437910853102925E-4	0.0
11	0.0	0.0	0.0	2.8613075194344094E-4	0.0
12	0.0	0.0	0.0	2.8613075194344094E-4	0.0
13	0.0	0.0	0.0	3.270065736496468E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	70	1.0932703E-4	12.212038	5
GGCCCGT	55	0.0030787243	12.085967	6
CCTAGAC	230	0.0	11.564744	3
GGACCGT	70	0.0014970297	10.852704	6
TGGCCCG	80	3.778761E-4	10.685534	5
CTAGACT	215	0.0	10.603785	4
CCAGTAC	325	0.0	10.522646	3
GTCTAGT	190	9.094947E-12	10.501074	1
CTAGACA	290	0.0	10.154342	4
CTAAGAC	205	4.1836756E-11	9.731309	3
GTCCAGG	450	0.0	9.712105	1
GGACTGT	325	0.0	9.642211	6
GGGTAGC	110	6.8313486E-5	9.500972	1
GTCCTAA	230	3.6379788E-12	9.500971	1
GTCTCGC	150	2.6456473E-7	9.500971	1
GGGCCGT	80	0.004532273	9.496117	6
TAAGACA	400	0.0	9.261743	4
TAAGACT	330	0.0	9.211368	4
AGGACCC	165	1.0691656E-7	9.210426	5
GTAAGAC	300	0.0	9.182958	3
>>END_MODULE