FastQCFastQC Report
Wed 25 May 2016
SRR1294719_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294719_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2446434
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT103540.4232282579460554No Hit
GGTATCAACGCAGAGTACTTTTTTT89080.3641218197588817No Hit
TATCAACGCAGAGTACTTTTTTTTT72320.2956139425792807No Hit
GTACATGGGAAGCAGTGGTATCAAC62460.25531038237696174No Hit
CCCATGTACTCTGCGTTGATACCAC55370.22632942478726178No Hit
ACGCAGAGTACTTTTTTTTTTTTTT42820.17503026854597342No Hit
GAGTACATGGGAAGCAGTGGTATCA32640.1334186820490559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC1001.4558282E-712.34208317
GTCCTAG2300.011.58811951
GCGCGAT1157.1429895E-811.55780316
GGCGCGA1157.1429895E-811.55780315
TTAGACT2400.011.1005034
CTAAGAC2600.010.2466183
TATTCCG856.502616E-410.0744075
GTTCTAG2950.010.0028181
TGGCGCG1252.5924128E-69.87366614
CTAGCCT2350.09.7171574
GACTGTG4850.09.5923737
GGCTAGA3100.09.5188111
AGCACCG800.0044596989.5147175
TTAGGCT2500.09.5147164
TATACTG6700.09.3727065
GTCTTGC3950.09.1573371
GTCCTGA3150.09.0655361
CTAATAC3050.09.046783
GGACAGT3050.09.0286576
AGCGTCA950.00179872079.0139423
Produced by FastQC (version 0.11.5)