FastQCFastQC Report
Wed 25 May 2016
SRR1294718_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294718_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3561063
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1325723.7228209666607976No Hit
CCCATGTACTCTGCGTTGATACCAC1271393.570254162872154No Hit
GAGTACATGGGAAGCAGTGGTATCA618131.735801922066529No Hit
CATGTACTCTGCGTTGATACCACTG502091.409944165548321No Hit
GCGTTGATACCACTGCTTCCCATGT391531.0994750724713378No Hit
GCTTCCCATGTACTCTGCGTTGATA362241.0172243512681467No Hit
ACGCAGAGTACATGGGAAGCAGTGG337600.9480315287878929No Hit
GTATCAACGCAGAGTACATGGGAAG320770.900770359861648No Hit
ACTCTGCGTTGATACCACTGCTTCC272140.7642100125720888No Hit
TATCAACGCAGAGTACATGGGAAGC257650.7235199152612577No Hit
GGTATCAACGCAGAGTACATGGGAA217880.6118397793018545No Hit
CAGTGGTATCAACGCAGAGTACATG181790.5104936363102816No Hit
GTGGTATCAACGCAGAGTACATGGG168250.47247128174929787No Hit
GCAGTGGTATCAACGCAGAGTACAT147430.4140055932736938No Hit
GTACATGGTAAGCAGTGGTATCAAC135680.3810098276834754No Hit
ATACCACTGCTTCCCATGTACTCTG132410.3718271763234742No Hit
CATGGGAAGCAGTGGTATCAACGCA129650.3640766815976016No Hit
GTACTCTGCGTTGATACCACTGCTT127060.35680357241643856No Hit
CTGCTTCCCATGTACTCTGCGTTGA123260.34613260141704877No Hit
ACATGGGAAGCAGTGGTATCAACGC122710.34458811877240025No Hit
GGGAAGCAGTGGTATCAACGCAGAG118600.3330466211914813No Hit
AAGCAGTGGTATCAACGCAGAGTAC104760.2941818215515985No Hit
CTTCCCATGTACTCTGCGTTGATAC102950.2890990695755734No Hit
GTTGATACCACTGCTTCCCATGTAC102890.28893058055979354No Hit
TACCACTGCTTCCCATGTACTCTGC102140.28682446786254556No Hit
GATACCACTGCTTCCCATGTACTCT102110.2867402233546556No Hit
GCAGAGTACATGGGAAGCAGTGGTA101990.28640324532309597No Hit
CCACTGCTTCCCATGTACTCTGCGT95670.2686557356609529No Hit
ACCATGTACTCTGCGTTGATACCAC94970.2666900304768548No Hit
CCATGTACTCTGCGTTGATACCACT90130.2530985832039478No Hit
GTACATGGGTAAGCAGTGGTATCAA89980.2526773606644982No Hit
ATGGGAAGCAGTGGTATCAACGCAG86200.24206255267036839No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA78040.2191480465243103No Hit
TTCCCATGTACTCTGCGTTGATACC77630.21799670491648138No Hit
TGATACCACTGCTTCCCATGTACTC68700.1929199230679154No Hit
GGTAAGCAGTGGTATCAACGCAGAG65220.18314756015268474No Hit
CCCCATGTACTCTGCGTTGATACCA63560.1784860307161092No Hit
GCTTACCATGTACTCTGCGTTGATA52250.1467258512416096No Hit
ATCAACGCAGAGTACATGGGAAGCA49610.1393123345472967No Hit
GAGTACATGGTAAGCAGTGGTATCA45930.12897834157946658No Hit
AAAAAGTACTCTGCGTTGATACCAC41780.11732451798802775No Hit
GGAAGCAGTGGTATCAACGCAGAGT39930.1121294400014827No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA37750.10600767242814856No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAC350.002172805816.283623
GCCCGTA500.001504369513.2951157
CGCAAAA3100.011.950582
GGTATCG1406.7484507E-1011.54201217
GTACAAA8300.011.3319381
GAACAAA17450.011.161061
AAAAGTA10600.011.0226312
AGTACTC11300.010.8429775
TATCGAC1502.3808752E-810.15156419
AAGTACT12200.010.1213654
CCGTACT1151.0292477E-59.911494
AAAAAGT11200.09.7549911
AAAGTAC11900.09.4987783
CTATACT3800.09.4985114
TTCCGTA900.00111800699.49651057
TAGGCAG2650.09.3189015
TATACTG5250.09.2267365
CCCGTAC1252.7603133E-59.1188273
TAGACTG3150.09.045825
GACAGTC2106.730261E-109.0442957
Produced by FastQC (version 0.11.5)