##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294718_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3561063
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.92913717055834	33.0	33.0	33.0	27.0	33.0
2	30.899790034604834	33.0	33.0	33.0	27.0	33.0
3	30.98773765024657	33.0	33.0	33.0	27.0	33.0
4	34.35363064343428	37.0	37.0	37.0	27.0	37.0
5	34.8162554271014	37.0	37.0	37.0	33.0	37.0
6	34.83192209741866	37.0	37.0	37.0	33.0	37.0
7	34.89831491327168	37.0	37.0	37.0	33.0	37.0
8	34.76160741890834	37.0	37.0	37.0	27.0	37.0
9	34.4819881029906	37.0	37.0	37.0	27.0	37.0
10	34.624471119999846	37.0	37.0	37.0	27.0	37.0
11	34.85064431603709	37.0	37.0	37.0	33.0	37.0
12	34.79899428906481	37.0	37.0	37.0	27.0	37.0
13	34.6326495768258	37.0	37.0	37.0	27.0	37.0
14	35.91143936515586	40.0	37.0	40.0	27.0	40.0
15	36.1159190387814	40.0	37.0	40.0	27.0	40.0
16	36.10861110853698	40.0	37.0	40.0	27.0	40.0
17	36.11715743304738	40.0	37.0	40.0	27.0	40.0
18	36.10212091164913	40.0	37.0	40.0	27.0	40.0
19	36.085541311681375	40.0	37.0	40.0	27.0	40.0
20	36.01287845792113	40.0	37.0	40.0	27.0	40.0
21	35.93857424033217	40.0	37.0	40.0	27.0	40.0
22	35.87084025191354	40.0	37.0	40.0	27.0	40.0
23	35.54001038453967	37.0	37.0	40.0	27.0	40.0
24	35.87472841676769	40.0	37.0	40.0	27.0	40.0
25	34.79736640435735	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5263930319143633
1101	2	0.6233557215987631
1101	3	0.4279890391711234
1101	4	0.6329780211893237
1101	5	0.5555849072250041
1101	6	0.3729775093169465
1101	7	0.4439671136485259
1101	8	0.45633262414176556
1101	9	0.763499871507932
1101	10	0.6795068452045996
1101	11	0.45839267306059384
1101	12	0.4951874254773969
1101	13	0.479804318831043
1101	14	1.1946536575606927
1101	15	0.9352001050478549
1101	16	0.8068213499498853
1101	17	0.9086984356901198
1101	18	0.8911411077517641
1101	19	0.9103396012406648
1101	20	1.1060188409471792
1101	21	1.0523583610242824
1101	22	1.0824584667851695
1101	23	1.41142269060731
1101	24	1.2423350453547002
1101	25	1.060056220602604
1102	1	0.3889157240619312
1102	2	0.5395156083404231
1102	3	0.4247316338823204
1102	4	0.4448253613679256
1102	5	0.45454473738600853
1102	6	0.37239043954293294
1102	7	0.3851296383949787
1102	8	0.46482048037474755
1102	9	0.5646120861675357
1102	10	0.5819732508199706
1102	11	0.38581066219141036
1102	12	0.5463839350028721
1102	13	0.5811215928806277
1102	14	1.2328661045769707
1102	15	0.9993542970345644
1102	16	0.6954770803179002
1102	17	0.9138557729669188
1102	18	0.9121569094004869
1102	19	0.8191363845121984
1102	20	1.0652980910382226
1102	21	1.0570569659442484
1102	22	1.0492254812338544
1102	23	1.3796005448906001
1102	24	1.1513558695219785
1102	25	1.1386880937042676
1103	1	0.45663628357861086
1103	2	0.5755049200754847
1103	3	0.4082035190052764
1103	4	0.3063106449513029
1103	5	0.4761666660482433
1103	6	0.31766576568309546
1103	7	0.35635651937161583
1103	8	0.4097835045107132
1103	9	0.7345099299791826
1103	10	0.6784456470537279
1103	11	0.498076132387439
1103	12	0.4593235362276147
1103	13	0.436675561063538
1103	14	1.1819311348442483
1103	15	0.9355687278620195
1103	16	0.688735248222919
1103	17	0.7074708499937685
1103	18	0.8028630976950737
1103	19	0.8194834612830704
1103	20	1.001749869897992
1103	21	1.0606598255855033
1103	22	0.9556417014970009
1103	23	1.3386357579385972
1103	24	1.0542766852910432
1103	25	1.0162487865741596
1104	1	0.39841815512600576
1104	2	0.5332980745212197
1104	3	0.46432564459390946
1104	4	0.525041794815813
1104	5	0.41661173769061577
1104	6	0.2879640551891569
1104	7	0.3803719768177487
1104	8	0.48028633439890456
1104	9	0.6998620091289851
1104	10	0.6506256269430537
1104	11	0.423677502190813
1104	12	0.38170196070888807
1104	13	0.516220334841627
1104	14	1.034973477441568
1104	15	0.8116017976867411
1104	16	0.6970620686348568
1104	17	0.7997485088539378
1104	18	0.7893744505338276
1104	19	0.7665438959538378
1104	20	0.8994734490301468
1104	21	0.895312947597148
1104	22	1.0294152143867947
1104	23	1.3702949306105623
1104	24	1.0138105264885695
1104	25	0.9149559864666017
1105	1	0.29253228687132093
1105	2	0.39166858956339823
1105	3	0.39789332628753016
1105	4	0.4348591139284821
1105	5	0.39447969681794604
1105	6	0.29906914200638823
1105	7	0.28608245824037226
1105	8	0.4292633943204933
1105	9	-1.4397734678378313
1105	10	0.013495390862289014
1105	11	0.19596917853046847
1105	12	0.30270125127339753
1105	13	0.33588168298873455
1105	14	-1.5118095745888596
1105	15	-0.11332135512246566
1105	16	0.06076857701902583
1105	17	0.32200686019774594
1105	18	0.2260489704167412
1105	19	0.32564845955614885
1105	20	0.5545135613416221
1105	21	0.6905821461646937
1105	22	0.658798075403837
1105	23	0.9531940404961787
1105	24	0.749265725961763
1105	25	0.5042724670874321
1106	1	0.05262886786847076
1106	2	0.09791234814103333
1106	3	0.16853184077287509
1106	4	-1.0026561655874673
1106	5	-0.20083055503630476
1106	6	0.02749965904003915
1106	7	0.1479469602156911
1106	8	0.2688882384555953
1106	9	0.41577009314525526
1106	10	0.46694252283579374
1106	11	0.3078804314966419
1106	12	0.15601668448312012
1106	13	0.18979025322082066
1106	14	0.5371288879001739
1106	15	0.36114303772021117
1106	16	0.27246309599674845
1106	17	0.15222450189965286
1106	18	0.2838597875840847
1106	19	0.2718262630959032
1106	20	0.465883784249705
1106	21	0.4931221304426572
1106	22	0.515018120279791
1106	23	0.6844846580408088
1106	24	0.48387436992816646
1106	25	0.3376202745775032
1107	1	0.30688298479157083
1107	2	0.31142035988382233
1107	3	0.23542307309212518
1107	4	0.5349579468383467
1107	5	0.43459727344230004
1107	6	0.45690988203772775
1107	7	0.35715310907550446
1107	8	0.4068674119314153
1107	9	0.5426760938775672
1107	10	0.532434629129888
1107	11	0.3115523321355198
1107	12	0.33584332001125006
1107	13	0.41084890265973684
1107	14	0.8788162932814032
1107	15	0.7363849150302855
1107	16	0.7258523313511631
1107	17	0.7241771824118004
1107	18	0.8182390118885365
1107	19	0.5808058246461982
1107	20	0.7346381170439429
1107	21	0.8221051368963685
1107	22	0.7041213168874876
1107	23	0.9511722997467587
1107	24	0.8890687957431638
1107	25	0.7960473256523386
1108	1	0.44137396339860047
1108	2	0.3471478825419183
1108	3	0.450874863243822
1108	4	0.529212405306211
1108	5	0.5250339613423165
1108	6	0.38359497869528525
1108	7	0.26145069076453353
1108	8	0.3113031949548457
1108	9	0.22502256847715785
1108	10	0.5505946934906518
1108	11	0.30981604513736727
1108	12	0.32544990320861444
1108	13	0.2875370512306574
1108	14	-1.0237144036182926
1108	15	-0.06308494874111403
1108	16	0.31119995372187503
1108	17	0.41476453100386124
1108	18	0.5779268703703693
1108	19	0.4740405350322092
1108	20	0.6116717296221026
1108	21	0.6227815289936132
1108	22	0.7440100604871205
1108	23	1.0064075267556234
1108	24	0.8870846587707746
1108	25	0.7095743495585722
1109	1	0.35814606158191964
1109	2	0.3287053501933528
1109	3	0.33711383327340627
1109	4	0.5157882141029475
1109	5	0.43879906966382975
1109	6	0.2153977445542239
1109	7	0.1522604632560629
1109	8	0.26186544837977266
1109	9	0.3891958930625705
1109	10	0.4394214471689182
1109	11	0.3278069096309082
1109	12	0.23107577891063613
1109	13	0.2740869415344491
1109	14	0.6231529907651208
1109	15	0.47994514923587417
1109	16	0.3830573872727072
1109	17	0.4314696984906874
1109	18	0.4526976695798055
1109	19	0.4146408759366196
1109	20	0.5958378808834439
1109	21	0.498010920361331
1109	22	0.6051093628730939
1109	23	0.990468161799356
1109	24	0.7950263009969802
1109	25	0.6377506481640083
1110	1	0.30240098970637064
1110	2	0.15197916645723808
1110	3	0.26454295995887733
1110	4	0.47427598923443526
1110	5	0.40779007833915415
1110	6	0.18920373844463967
1110	7	0.21916653743803494
1110	8	0.2937559790049846
1110	9	0.40129586639068293
1110	10	0.33519610265069133
1110	11	0.18691485957302234
1110	12	0.10421299420196561
1110	13	0.12105635864134712
1110	14	0.4930638098355118
1110	15	0.29237239559676453
1110	16	0.3031090457265151
1110	17	0.35005515519889485
1110	18	0.41235376642724475
1110	19	0.3290576423877667
1110	20	0.43427085010217326
1110	21	0.45299968669159796
1110	22	0.5559204730771725
1110	23	0.706519399923188
1110	24	0.6216160467763387
1110	25	0.5477137601958617
1111	1	0.2015406887268476
1111	2	0.22831266519765947
1111	3	0.27909016035953726
1111	4	0.5885802345500295
1111	5	0.30063464140206264
1111	6	0.29158894546588243
1111	7	0.3184623144631473
1111	8	0.2571612107176051
1111	9	0.27913383311715734
1111	10	0.1664889446973632
1111	11	0.13141123795569598
1111	12	0.21153899377172536
1111	13	0.21018650455863508
1111	14	-1.8612261411162265
1111	15	-0.5420877620094942
1111	16	-0.019553627847358257
1111	17	0.14465168312307242
1111	18	0.186916550078152
1111	19	0.2396130388783746
1111	20	0.19591509474877
1111	21	0.37669902386642207
1111	22	0.16838839284829277
1111	23	-1.7110704316224314
1111	24	-0.34498694276496167
1111	25	0.05706871245029532
1112	1	-0.19317221956659125
1112	2	-0.21581202101175023
1112	3	-0.034233472077858096
1112	4	0.005248134882258171
1112	5	-0.7113086300751306
1112	6	-0.7623962892563512
1112	7	-0.8464186988623652
1112	8	-0.6027014431526254
1112	9	-0.5627244247668131
1112	10	-0.7310551743597884
1112	11	-0.7142135264843574
1112	12	-0.6902871415293248
1112	13	-0.7761841355601433
1112	14	-0.4566785691251951
1112	15	-0.7873606584360999
1112	16	-0.6190820277890268
1112	17	-0.6625284868627404
1112	18	-0.8333808977706667
1112	19	-0.6155097684657633
1112	20	-0.8065661243551148
1112	21	-1.7515737689913777
1112	22	-1.5771229482318745
1112	23	-1.200526136897217
1112	24	-1.516772789651256
1112	25	-1.7313946972506287
1113	1	-0.058771028731953834
1113	2	-0.08135797022023894
1113	3	-0.19421318145519706
1113	4	-0.023710570608493242
1113	5	-0.010703539039404575
1113	6	-0.13362321549649891
1113	7	-0.12795678886628536
1113	8	-0.0373516803919145
1113	9	0.0650382637389626
1113	10	-0.2980630295912263
1113	11	-0.2882641936167474
1113	12	-0.16568558209149842
1113	13	-0.2483525909144646
1113	14	-0.14097662258318877
1113	15	-0.36955426372873035
1113	16	-0.2076775611917725
1113	17	-0.24341812684670572
1113	18	-0.09197379335791567
1113	19	-0.17491667283985635
1113	20	-0.7821939702203125
1113	21	-0.5721581790008372
1113	22	-0.16688606479964818
1113	23	-0.3631824430123345
1113	24	-0.07318101431505397
1113	25	-1.2972405835281293
1114	1	-1.0563443387194411
1114	2	-0.7981189563497715
1114	3	-0.6010733142899767
1114	4	-1.6825600132048706
1114	5	-0.876134972551796
1114	6	-0.761207844046325
1114	7	-0.6036882445077936
1114	8	-0.5942324596950286
1114	9	-0.618278448662906
1114	10	-0.6718799398964208
1114	11	-0.6681784315381094
1114	12	-0.5795027781531203
1114	13	-0.7455830498954086
1114	14	-0.5905502246772443
1114	15	-1.4220920273751716
1114	16	-0.9087806263319322
1114	17	-0.9408897891932924
1114	18	-0.9371780380427452
1114	19	-0.9690828081073022
1114	20	-2.208566390552818
1114	21	-6.901022514382717
1114	22	-7.891914596500953
1114	23	-7.994014744305737
1114	24	-5.866461531470673
1114	25	-7.264312684125279
1115	1	-1.3200918797594738
1115	2	-1.2474799073546414
1115	3	-0.9120186698206076
1115	4	-0.8955618786098825
1115	5	-0.9824200330745683
1115	6	-1.0439450284595324
1115	7	-1.2201765391475021
1115	8	-0.9322654713027418
1115	9	-2.8495112247192793
1115	10	-1.754109656329419
1115	11	-1.369322292200657
1115	12	-1.13416557982233
1115	13	-1.6758035837170056
1115	14	-1.4743417782011932
1115	15	-2.2572184341920973
1115	16	-1.550492294429013
1115	17	-3.22974696860814
1115	18	-3.1916195052552396
1115	19	-2.3348372495745195
1115	20	-1.9932303370554223
1115	21	-1.5184113364688727
1115	22	-1.143877087867935
1115	23	-0.5793658153235697
1115	24	-1.5022728180314502
1115	25	-1.3173162256262358
1116	1	-1.5841594257356206
1116	2	-0.8707710986789436
1116	3	-0.6978041138045086
1116	4	-0.43447295716595136
1116	5	-0.42603937411573156
1116	6	-0.6202721646193083
1116	7	-0.9615090829459803
1116	8	-1.8750805939673398
1116	9	-1.0160510653768995
1116	10	-2.6232863340146864
1116	11	-1.1733075918636402
1116	12	-0.9740791012595551
1116	13	-0.9452578799990903
1116	14	-1.3652624871260457
1116	15	-1.3251330950624833
1116	16	-0.9415680140433267
1116	17	-0.8632161442186685
1116	18	-1.4993267592158688
1116	19	-0.9896333554925647
1116	20	-1.3481685871390496
1116	21	-0.7579074598676883
1116	22	-0.4337267951060113
1116	23	-0.209452647382264
1116	24	-0.28219129083691286
1116	25	-0.3660885973552723
1201	1	0.34482623572099413
1201	2	0.4058610921879513
1201	3	0.41647878056743437
1201	4	0.5197722279210524
1201	5	0.5064300059415459
1201	6	0.37924530935460155
1201	7	0.39817770746000747
1201	8	0.4163331875162015
1201	9	0.7819382642896215
1201	10	0.6539616321823445
1201	11	0.4538497624607061
1201	12	0.46756395381587623
1201	13	0.49960366857833804
1201	14	1.0933361397291534
1201	15	0.9008724034821185
1201	16	0.9473940941912318
1201	17	0.8259446985161532
1201	18	0.7806384739762819
1201	19	0.8175898225133906
1201	20	0.9236677682377774
1201	21	0.8669117311153229
1201	22	0.9688347015759078
1201	23	0.7829103845823155
1201	24	0.825567984375347
1201	25	0.7693845893222786
1202	1	0.026827875823155267
1202	2	0.2220443339066307
1202	3	0.14823757202422883
1202	4	0.23286013728724697
1202	5	0.30929691765619793
1202	6	0.20607311706220344
1202	7	0.3109964765365021
1202	8	0.31740604356995306
1202	9	0.3749178385748735
1202	10	0.5374395102413203
1202	11	0.406909841560136
1202	12	0.24376914873816702
1202	13	0.42050445809424275
1202	14	0.562739181602133
1202	15	0.6758638205971792
1202	16	0.6296651301075755
1202	17	0.4632876673127839
1202	18	0.5097713498112739
1202	19	0.5398337947364169
1202	20	0.6721134401731774
1202	21	0.7382625996339272
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2211	5	0.33132132940003345
2211	6	0.2948668262613623
2211	7	0.28496538212881006
2211	8	0.3971988576653587
2211	9	0.5517583652963012
2211	10	0.5460759494194534
2211	11	0.33582324780409323
2211	12	0.31359054589696456
2211	13	0.20542502128069628
2211	14	0.49961026430835886
2211	15	0.35255049832387186
2211	16	0.33328412885481384
2211	17	0.4452329095107572
2211	18	0.4135982342184903
2211	19	0.4126799817013378
2211	20	0.42878536472399986
2211	21	0.5389805292132621
2211	22	0.47265834152629793
2211	23	0.6743701424050101
2211	24	0.6651918949887943
2211	25	0.6212518902244355
2212	1	-0.3450789878984679
2212	2	-0.3148026494225391
2212	3	-0.23439913093193
2212	4	-0.5128032393176483
2212	5	-0.2721686156045351
2212	6	-0.6067805460840106
2212	7	-0.523892727167798
2212	8	-0.3194840756941275
2212	9	-0.19928729507694953
2212	10	-0.43864472266736954
2212	11	-0.4083416793382497
2212	12	-0.546944278056948
2212	13	-0.6550866090155125
2212	14	-0.33444253940013624
2212	15	-0.5754363077537974
2212	16	-0.46382366872837366
2212	17	-0.5074503164935393
2212	18	-0.38281048472886425
2212	19	-0.4702018536174677
2212	20	-0.5768844727803
2212	21	-0.4727425416270137
2212	22	-0.5418711618959335
2212	23	0.014216846337191669
2212	24	-0.263080862425781
2212	25	-0.8227544172051608
2213	1	-0.1730803233837932
2213	2	-1.2652772987841843
2213	3	-0.29561356586436816
2213	4	0.10110340629920245
2213	5	-0.020879484113820013
2213	6	0.017891725724055618
2213	7	-0.07011575167091877
2213	8	0.1936549442148987
2213	9	0.09707793015939359
2213	10	-0.32232995081715643
2213	11	-0.11610306664708503
2213	12	-0.007781727564513119
2213	13	-0.3409870788632503
2213	14	-0.027044900190560384
2213	15	-0.1045008510049854
2213	16	-0.045218422015395277
2213	17	-0.001413585698522013
2213	18	0.05457487747978007
2213	19	0.09091835815500104
2213	20	0.2302376413937992
2213	21	-0.5720021637786914
2213	22	-0.8946374459327586
2213	23	-0.11159286123079681
2213	24	-0.059576589754193776
2213	25	-0.562556163511509
2214	1	-0.31579519487570806
2214	2	-0.5614418111965804
2214	3	-0.5051991025784659
2214	4	-0.7274066452394052
2214	5	-0.44233375928876484
2214	6	-0.2489846773056712
2214	7	-0.6006429628750212
2214	8	-0.23445981137886918
2214	9	-0.16971143088699137
2214	10	-1.9506773382972895
2214	11	-0.9185378746387372
2214	12	-0.6326438426178598
2214	13	-0.3636727543849432
2214	14	-0.9835242149069359
2214	15	-0.6765949126109305
2214	16	-0.8694943615361197
2214	17	-0.7267361598071176
2214	18	-0.7553425329084078
2214	19	-1.6657980728507198
2214	20	-2.7743971422818063
2214	21	-2.0838287460661604
2214	22	-2.278958636143443
2214	23	-2.7133796869182945
2214	24	-1.8537126340066195
2214	25	-2.098053290917157
2215	1	-0.24375221442848272
2215	2	-0.3912321634780831
2215	3	-0.9032836453391617
2215	4	-0.40190708237173567
2215	5	-0.3240309601070521
2215	6	-0.29091832304514753
2215	7	-0.3216861478661315
2215	8	-0.33502435809091935
2215	9	-0.34439517617767024
2215	10	-0.39466688730452404
2215	11	-0.7732019357195057
2215	12	-0.506631329443195
2215	13	-0.24614270136259364
2215	14	-0.22997149964960784
2215	15	-0.6969004838785722
2215	16	-0.5127441760374154
2215	17	-0.3662973475904039
2215	18	-0.4631678295458812
2215	19	-2.350956558087425
2215	20	-2.5031846993162077
2215	21	-3.4086130192363413
2215	22	-2.5453749853671113
2215	23	-3.4408133754919916
2215	24	-2.564895659486986
2215	25	-1.8625045119437118
2216	1	-1.650223259318178
2216	2	-1.2334113559264033
2216	3	-0.9884302057020236
2216	4	-0.9959509989641546
2216	5	-1.927730687148184
2216	6	-1.6185027365181384
2216	7	-1.2750094396003462
2216	8	-5.282810690503524
2216	9	-4.210351337280279
2216	10	-2.210421189188324
2216	11	-1.1953442415838396
2216	12	-1.3943550860487974
2216	13	-2.1768369684726494
2216	14	-3.4593825058228305
2216	15	-2.1459516217599344
2216	16	-3.168546014522093
2216	17	-2.8644187803957095
2216	18	-2.8345762391976663
2216	19	-1.9809834770951724
2216	20	-1.1752719953616833
2216	21	-1.0003865885592305
2216	22	-0.961776701360364
2216	23	-1.2600239464686922
2216	24	-0.7221980556654088
2216	25	-0.6291778793233149
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	45.0
8	235.0
9	685.0
10	1358.0
11	2748.0
12	4834.0
13	7487.0
14	10911.0
15	16069.0
16	20767.0
17	22359.0
18	22958.0
19	23347.0
20	23757.0
21	25281.0
22	25938.0
23	26989.0
24	28629.0
25	30097.0
26	31546.0
27	34510.0
28	38958.0
29	45504.0
30	54597.0
31	66058.0
32	88659.0
33	127679.0
34	186432.0
35	298340.0
36	526528.0
37	1767753.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.44987738660719	19.192970727281413	11.139335443838009	24.217816442273392
2	12.92809784564001	22.819274678862545	36.48748352430118	27.765143951196265
3	21.308777660591883	28.535998618111446	27.087487308111996	23.06773641318468
4	13.182195445640495	19.296159760696003	32.92219109210536	34.59945370155814
5	11.67745728155149	40.480801449971025	34.63420840721424	13.20753286126324
6	32.72716345917572	33.038434568055166	18.075039252904407	16.15936271986471
7	24.673228401735443	33.74577722862639	22.471799048033585	19.109195321604584
8	26.814154918265725	36.15212583247872	21.213787386424013	15.819931862831545
9	26.596917536635285	17.886672687366193	20.14443500901982	35.37197476697871
10	16.231089554489518	32.20952968792166	31.916302472774266	19.643078284814557
11	29.342908107330924	27.907702952858504	19.346232993655757	23.403155946154815
12	23.14508411063784	31.345983627181397	28.340836793012347	17.168095469168417
13	28.6564980461702	22.672709989314665	22.72491508598115	25.94587687853399
14	21.107642089028996	26.700274807205304	25.596721409070717	26.59536169469498
15	30.636054528748236	25.501350188599552	21.224161636221897	22.63843364643031
16	19.334729960460102	29.380756874550684	30.638114441049602	20.646398723939612
17	25.290876290802156	30.08207889773668	27.019649735296397	17.60739507616477
18	25.56377274137769	26.394362202380535	28.2026080009211	19.839257055320676
19	22.875366219335934	31.355901028039774	28.709574060901716	17.05915869172258
20	23.470975514210014	28.023796385611526	27.220380534365614	21.284847565812846
21	19.37731502235378	30.14428566971828	29.901228523464376	20.577170784463565
22	21.6676461074046	29.293765613788953	22.864137657936574	26.17445062086987
23	18.217102630170743	30.59026513056421	27.139683695999206	24.05294854326584
24	20.7920694766059	34.90291842856525	24.42357904716225	19.8814330476666
25	22.1534326383856	27.029925204579197	22.303389365399905	28.513252791635296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	10254.0
1	10254.0
2	12485.5
3	14717.0
4	14717.0
5	14717.0
6	18032.0
7	21347.0
8	21347.0
9	21347.0
10	22275.0
11	23203.0
12	23203.0
13	23203.0
14	27587.5
15	31972.0
16	31972.0
17	31972.0
18	42652.5
19	53333.0
20	53333.0
21	53333.0
22	70182.5
23	87032.0
24	87032.0
25	87032.0
26	110095.0
27	133158.0
28	133158.0
29	133158.0
30	156762.5
31	180367.0
32	180367.0
33	180367.0
34	202549.5
35	224732.0
36	224732.0
37	224732.0
38	246582.5
39	268433.0
40	268433.0
41	268433.0
42	314028.5
43	359624.0
44	359624.0
45	359624.0
46	607989.0
47	856354.0
48	856354.0
49	856354.0
50	736405.0
51	616456.0
52	616456.0
53	616456.0
54	451900.0
55	287344.0
56	287344.0
57	287344.0
58	226032.5
59	164721.0
60	164721.0
61	164721.0
62	138136.5
63	111552.0
64	111552.0
65	111552.0
66	88121.0
67	64690.0
68	64690.0
69	64690.0
70	47839.5
71	30989.0
72	30989.0
73	30989.0
74	22277.0
75	13565.0
76	13565.0
77	13565.0
78	9258.5
79	4952.0
80	4952.0
81	4952.0
82	3289.5
83	1627.0
84	1627.0
85	1627.0
86	1045.5
87	464.0
88	464.0
89	464.0
90	298.5
91	133.0
92	133.0
93	133.0
94	82.5
95	32.0
96	32.0
97	32.0
98	22.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03069308237456063
2	0.014490055357066134
3	0.020162518888320706
4	0.02072414894092017
5	0.02010635588306076
6	0.005054670473395163
7	3.650595341896507E-4
8	3.088965289297044E-4
9	4.21222539449597E-4
10	5.616300525994626E-5
11	0.001095178602568952
12	0.001095178602568952
13	0.003032802284037098
14	5.897115552294357E-4
15	0.002218438707767877
16	0.0028923947708872325
17	0.0027800687603673395
18	0.0027519872577373666
19	3.9314103681962375E-4
20	5.054670473395163E-4
21	0.08570474602667799
22	0.0817171726532218
23	0.08331781830313027
24	0.0010390155973090058
25	0.225719118139724
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3561063.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.00034125780814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.90499884564336	47.11995278344136
2	7.616278974523569	8.073307695288362
3	1.5520779703606642	2.467819862635243
4	0.5916626442742969	1.254332882241395
5	0.2980331037769085	0.7897928103149949
6	0.2015234361414919	0.6408486532167106
7	0.1374776139489544	0.510045231822265
8	0.09424616355173628	0.39960630643849765
9	0.07248273799106435	0.34574488639440343
>10	0.45931666793354914	4.59878678824776
>50	0.039471920423913766	1.412388149793509
>100	0.023825955889602644	2.451481328585192
>500	0.00328026186249993	1.245542807893215
>1k	0.0032802618624713353	3.7715989290167014
>5k	5.915226309374539E-4	2.457768157933057
>10k+	0.001451919185028296	22.460982726737395
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	132572	3.7228209666607976	No Hit
CCCATGTACTCTGCGTTGATACCAC	127139	3.570254162872154	No Hit
GAGTACATGGGAAGCAGTGGTATCA	61813	1.735801922066529	No Hit
CATGTACTCTGCGTTGATACCACTG	50209	1.409944165548321	No Hit
GCGTTGATACCACTGCTTCCCATGT	39153	1.0994750724713378	No Hit
GCTTCCCATGTACTCTGCGTTGATA	36224	1.0172243512681467	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	33760	0.9480315287878929	No Hit
GTATCAACGCAGAGTACATGGGAAG	32077	0.900770359861648	No Hit
ACTCTGCGTTGATACCACTGCTTCC	27214	0.7642100125720888	No Hit
TATCAACGCAGAGTACATGGGAAGC	25765	0.7235199152612577	No Hit
GGTATCAACGCAGAGTACATGGGAA	21788	0.6118397793018545	No Hit
CAGTGGTATCAACGCAGAGTACATG	18179	0.5104936363102816	No Hit
GTGGTATCAACGCAGAGTACATGGG	16825	0.47247128174929787	No Hit
GCAGTGGTATCAACGCAGAGTACAT	14743	0.4140055932736938	No Hit
GTACATGGTAAGCAGTGGTATCAAC	13568	0.3810098276834754	No Hit
ATACCACTGCTTCCCATGTACTCTG	13241	0.3718271763234742	No Hit
CATGGGAAGCAGTGGTATCAACGCA	12965	0.3640766815976016	No Hit
GTACTCTGCGTTGATACCACTGCTT	12706	0.35680357241643856	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	12326	0.34613260141704877	No Hit
ACATGGGAAGCAGTGGTATCAACGC	12271	0.34458811877240025	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	11860	0.3330466211914813	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	10476	0.2941818215515985	No Hit
CTTCCCATGTACTCTGCGTTGATAC	10295	0.2890990695755734	No Hit
GTTGATACCACTGCTTCCCATGTAC	10289	0.28893058055979354	No Hit
TACCACTGCTTCCCATGTACTCTGC	10214	0.28682446786254556	No Hit
GATACCACTGCTTCCCATGTACTCT	10211	0.2867402233546556	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	10199	0.28640324532309597	No Hit
CCACTGCTTCCCATGTACTCTGCGT	9567	0.2686557356609529	No Hit
ACCATGTACTCTGCGTTGATACCAC	9497	0.2666900304768548	No Hit
CCATGTACTCTGCGTTGATACCACT	9013	0.2530985832039478	No Hit
GTACATGGGTAAGCAGTGGTATCAA	8998	0.2526773606644982	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	8620	0.24206255267036839	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	7804	0.2191480465243103	No Hit
TTCCCATGTACTCTGCGTTGATACC	7763	0.21799670491648138	No Hit
TGATACCACTGCTTCCCATGTACTC	6870	0.1929199230679154	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	6522	0.18314756015268474	No Hit
CCCCATGTACTCTGCGTTGATACCA	6356	0.1784860307161092	No Hit
GCTTACCATGTACTCTGCGTTGATA	5225	0.1467258512416096	No Hit
ATCAACGCAGAGTACATGGGAAGCA	4961	0.1393123345472967	No Hit
GAGTACATGGTAAGCAGTGGTATCA	4593	0.12897834157946658	No Hit
AAAAAGTACTCTGCGTTGATACCAC	4178	0.11732451798802775	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	3993	0.1121294400014827	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	3775	0.10600767242814856	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.808150262997313E-5	0.0
3	0.0	0.0	0.0	5.616300525994626E-5	0.0
4	0.0	0.0	0.0	5.616300525994626E-5	0.0
5	0.0	0.0	0.0	5.616300525994626E-5	0.0
6	0.0	0.0	0.0	5.616300525994626E-5	0.0
7	0.0	0.0	0.0	5.616300525994626E-5	0.0
8	0.0	0.0	0.0	5.616300525994626E-5	0.0
9	0.0	0.0	0.0	1.1232601051989251E-4	0.0
10	0.0	0.0	0.0	1.1232601051989251E-4	0.0
11	0.0	0.0	0.0	1.1232601051989251E-4	0.0
12	0.0	2.808150262997313E-5	0.0	1.4040751314986564E-4	0.0
13	0.0	2.808150262997313E-5	0.0	1.965705184098119E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGGTAC	35	0.0021728058	16.28362	3
GCCCGTA	50	0.0015043695	13.295115	7
CGCAAAA	310	0.0	11.95058	2
GGTATCG	140	6.7484507E-10	11.542012	17
GTACAAA	830	0.0	11.331938	1
GAACAAA	1745	0.0	11.16106	1
AAAAGTA	1060	0.0	11.022631	2
AGTACTC	1130	0.0	10.842977	5
TATCGAC	150	2.3808752E-8	10.151564	19
AAGTACT	1220	0.0	10.121365	4
CCGTACT	115	1.0292477E-5	9.91149	4
AAAAAGT	1120	0.0	9.754991	1
AAAGTAC	1190	0.0	9.498778	3
CTATACT	380	0.0	9.498511	4
TTCCGTA	90	0.0011180069	9.4965105	7
TAGGCAG	265	0.0	9.318901	5
TATACTG	525	0.0	9.226736	5
CCCGTAC	125	2.7603133E-5	9.118827	3
TAGACTG	315	0.0	9.04582	5
GACAGTC	210	6.730261E-10	9.044295	7
>>END_MODULE