FastQCFastQC Report
Wed 25 May 2016
SRR1294718_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294718_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3561063
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1394993.917341535378621No Hit
GTACATGGGAAGCAGTGGTATCAAC1287173.6145667740222516No Hit
GAGTACATGGGAAGCAGTGGTATCA676811.9005841794992113No Hit
CATGTACTCTGCGTTGATACCACTG576821.6197972347021101No Hit
GCGTTGATACCACTGCTTCCCATGT424161.1911050155529403No Hit
ACGCAGAGTACATGGGAAGCAGTGG411781.1563401152970336No Hit
GCTTCCCATGTACTCTGCGTTGATA410841.153700454049816No Hit
GTATCAACGCAGAGTACATGGGAAG335890.9432295918381675No Hit
ACTCTGCGTTGATACCACTGCTTCC315490.8859433264730222No Hit
TATCAACGCAGAGTACATGGGAAGC296420.8323919009576636No Hit
GGTATCAACGCAGAGTACATGGGAA242720.6815942318347078No Hit
CAGTGGTATCAACGCAGAGTACATG216210.607150168362649No Hit
GTGGTATCAACGCAGAGTACATGGG176800.49648096649792495No Hit
GCAGTGGTATCAACGCAGAGTACAT166580.46778167081009236No Hit
CATGGGAAGCAGTGGTATCAACGCA164070.4607332136499691No Hit
ACATGGGAAGCAGTGGTATCAACGC156680.43998098320641893No Hit
ATACCACTGCTTCCCATGTACTCTG151020.42408685271785423No Hit
GGGAAGCAGTGGTATCAACGCAGAG140700.3951067420037219No Hit
CTGCTTCCCATGTACTCTGCGTTGA134060.3764606242574198No Hit
GTACATGGTAAGCAGTGGTATCAAC130450.3663232018079995No Hit
AAGCAGTGGTATCAACGCAGAGTAC127710.35862887008738686No Hit
CTTCCCATGTACTCTGCGTTGATAC126330.35475362272445055No Hit
GTACTCTGCGTTGATACCACTGCTT123980.34815446960640684No Hit
TACCACTGCTTCCCATGTACTCTGC115940.32557694149190847No Hit
GATACCACTGCTTCCCATGTACTCT114460.3214208791026724No Hit
GCAGAGTACATGGGAAGCAGTGGTA113470.31864081034230507No Hit
GTTGATACCACTGCTTCCCATGTAC108740.3053582595983278No Hit
CCATGTACTCTGCGTTGATACCACT108620.30502128156676817No Hit
CCACTGCTTCCCATGTACTCTGCGT107290.3012864417169817No Hit
ACCATGTACTCTGCGTTGATACCAC107250.3011741157064618No Hit
ATGGGAAGCAGTGGTATCAACGCAG103180.2897449441360628No Hit
GTACATGGGTAAGCAGTGGTATCAA98390.2762939043763056No Hit
GTATCAACGCAGAGTACTTTTTTTT94620.26570717788480575No Hit
TTCCCATGTACTCTGCGTTGATACC79700.22380957596088583No Hit
TATCAACGCAGAGTACTTTTTTTTT76370.2144584355851048No Hit
GGTAAGCAGTGGTATCAACGCAGAG75720.21263313791415653No Hit
GGTATCAACGCAGAGTACTTTTTTT74570.20940376511170963No Hit
CCCCATGTACTCTGCGTTGATACCA74260.20853323853018044No Hit
TGATACCACTGCTTCCCATGTACTC72360.20319775303048554No Hit
GCTTACCATGTACTCTGCGTTGATA58800.16511923546424198No Hit
ATCAACGCAGAGTACATGGGAAGCA57410.1612159065986757No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA55380.15551536156479118No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55280.15523454653849145No Hit
ACGCAGAGTACTTTTTTTTTTTTTT54140.15203325523867453No Hit
GAGTACATGGTAAGCAGTGGTATCA51420.14439508652332184No Hit
GGAAGCAGTGGTATCAACGCAGAGT48780.13698156982900894No Hit
AAAAAGTACTCTGCGTTGATACCAC47890.1344823160949413No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39500.11092193538839387No Hit
ACTCTGCGTTGATACCACTGCTTAC38470.10802954061750662No Hit
ATGGTAAGCAGTGGTATCAACGCAG37590.105558368386069No Hit
TGGGAAGCAGTGGTATCAACGCAGA37400.10502481983609951No Hit
TCCATGTACTCTGCGTTGATACCAC35780.10047561641004386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG500.001485453613.3174585
CGCAAAA1156.9559974E-811.5803992
GTTACTC1354.640242E-911.2740383
GGCGCGA600.005901385611.07746915
GGACCGC803.7878155E-410.6830456
CGAGTGC856.6295755E-410.05350115
AAAAAGT11000.010.0350031
GAACAAA9450.09.9690911
CTACACT3650.09.9033944
TGGACGG1252.5297104E-69.8929685
AGTACTC14700.09.8360235
CACGTAC1752.5193003E-99.7842553
CGTACTC1856.348273E-109.7695645
AAAAGTA11500.09.7606212
CTAGGAC2251.8189894E-129.7238593
GTGTAAG3700.09.515951
GTCTTAA2002.564775E-109.515951
GCTAGAC1401.0408694E-69.512473
CTTAGAC1901.029548E-99.5124693
CGTGTGG1002.7709926E-49.4952410
Produced by FastQC (version 0.11.5)