FastQCFastQC Report
Wed 25 May 2016
SRR1294717_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294717_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857308
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44030.1540960932458104No Hit
GGTATCAACGCAGAGTACTTTTTTT35580.12452280258201077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGC500.001504736113.2945366
TACGTCG604.0996246E-412.6645665
CGTAGCA1103.8216967E-812.0901742
AGGCGTC700.001498062210.8519237
CTAGGAC2900.010.1545553
GTCTAGG3200.010.0952571
CAGAACG1054.111808E-59.9514274
GTCTAGC1856.475602E-109.7582131
CGATTTC1201.6899901E-59.50541216
GTCCTAT2700.09.5014181
TGAACCG900.00111580599.4984255
TCGTTTG900.00111884649.49559913
TGTGCGA800.00453535849.49543310
TATACTG8950.09.4453615
AGGACTG6600.09.3545095
TATTAGG2750.09.3267052
TTAGACT2252.7284841E-119.2879994
ACGTAGC1151.1093497E-49.0883131
TAGGACA3150.09.0467524
ATCCCGT950.00183126598.9963036
Produced by FastQC (version 0.11.5)