##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294717_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2857308
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.977923626014416	33.0	33.0	33.0	27.0	33.0
2	30.956629106837624	33.0	33.0	33.0	27.0	33.0
3	30.869491843371453	33.0	33.0	33.0	27.0	33.0
4	34.35263331779423	37.0	37.0	37.0	27.0	37.0
5	34.526585163377554	37.0	37.0	37.0	27.0	37.0
6	34.772838979906965	37.0	37.0	37.0	27.0	37.0
7	34.705734208562745	37.0	37.0	37.0	27.0	37.0
8	34.63773698880205	37.0	37.0	37.0	27.0	37.0
9	34.374422708367455	37.0	37.0	37.0	27.0	37.0
10	34.44856032321332	37.0	37.0	37.0	27.0	37.0
11	34.68701029080519	37.0	37.0	37.0	27.0	37.0
12	34.604337719279826	37.0	37.0	37.0	27.0	37.0
13	34.53211204392386	37.0	37.0	37.0	27.0	37.0
14	35.643923931196774	37.0	37.0	40.0	27.0	40.0
15	35.973953455490275	40.0	37.0	40.0	27.0	40.0
16	35.98594936212687	40.0	37.0	40.0	27.0	40.0
17	35.92073903128399	40.0	37.0	40.0	27.0	40.0
18	35.91518835211325	37.0	37.0	40.0	27.0	40.0
19	35.99810345962003	40.0	37.0	40.0	27.0	40.0
20	35.91149466560833	40.0	37.0	40.0	27.0	40.0
21	35.85550035207965	40.0	37.0	40.0	27.0	40.0
22	35.70846160091946	37.0	37.0	40.0	27.0	40.0
23	35.30462519266386	37.0	33.0	40.0	27.0	40.0
24	35.6282035398354	37.0	37.0	40.0	27.0	40.0
25	34.41477887578098	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.3852785057435959
1101	2	0.5058959216428072
1101	3	0.308092237259487
1101	4	0.4739904681130582
1101	5	0.5123711054570776
1101	6	0.29617980637317487
1101	7	0.3585917583583438
1101	8	0.4601478827808734
1101	9	0.5347727986402617
1101	10	0.618204302053627
1101	11	0.38309838613786695
1101	12	0.4462270225675624
1101	13	0.4002694862110019
1101	14	1.1124639564190986
1101	15	0.8216085718659016
1101	16	0.7037935156649127
1101	17	0.7884247785405094
1101	18	0.6517635600431717
1101	19	0.657729631121029
1101	20	0.7494743700064461
1101	21	0.7930961637729936
1101	22	0.8803212349163232
1101	23	1.1732559493400032
1101	24	1.004287076361635
1101	25	0.7396625827817331
1102	1	0.3434555908937682
1102	2	0.47731715064461966
1102	3	0.21087344373919592
1102	4	0.3064451220392641
1102	5	0.21870831933562584
1102	6	0.1792939785697456
1102	7	0.2736455189139022
1102	8	0.33167458908929603
1102	9	0.5317806040056752
1102	10	0.664461199334788
1102	11	0.4200944340371251
1102	12	0.37033523955609127
1102	13	0.3053346553995553
1102	14	1.0241734468275254
1102	15	0.8101326319617996
1102	16	0.5189002049431224
1102	17	0.7199262458758611
1102	18	0.6501012918047877
1102	19	0.613466169962706
1102	20	0.7080962733295735
1102	21	0.7835357315597733
1102	22	0.8498180117874341
1102	23	1.2081071764724243
1102	24	0.9721749721195536
1102	25	0.7881599504330978
1103	1	0.06477992421125478
1103	2	0.3214621042633574
1103	3	0.08592675473869704
1103	4	-0.14636324434993497
1103	5	0.08418315759650596
1103	6	-0.013181139916689233
1103	7	-2.74291370409685E-4
1103	8	0.2080182783578266
1103	9	0.40238908791453554
1103	10	0.4969205037482638
1103	11	0.2139283195665982
1103	12	0.2384286742227104
1103	13	0.15654580263674944
1103	14	0.7741577402353101
1103	15	0.5494136722600942
1103	16	0.2851653368735114
1103	17	0.1773647605231048
1103	18	0.37640990578750433
1103	19	0.3954185586555994
1103	20	0.4931064889857879
1103	21	0.5852706146988496
1103	22	0.4437493058898525
1103	23	0.9470884895612599
1103	24	0.6179682148610368
1103	25	0.459887186927169
1104	1	0.2090839645932121
1104	2	0.4190146613817305
1104	3	0.1760799426816142
1104	4	0.15006278989336863
1104	5	0.12390792323456878
1104	6	0.20877078571464835
1104	7	0.10702351783596953
1104	8	0.34037925062173713
1104	9	0.524089355053249
1104	10	0.5451769471972057
1104	11	0.3491014584453822
1104	12	0.307800272190093
1104	13	0.377362880081364
1104	14	0.8712667591978089
1104	15	0.6840281098521714
1104	16	0.500221055733256
1104	17	0.5788451864345632
1104	18	0.5301483792610071
1104	19	0.4223012550142613
1104	20	0.6824375468811539
1104	21	0.7599776112068568
1104	22	0.6999125208646433
1104	23	1.1054011365930023
1104	24	0.9676943518567995
1104	25	0.7041323980511152
1105	1	0.22533150414702874
1105	2	0.3087312718487283
1105	3	0.30079557761960984
1105	4	0.20697543658128836
1105	5	0.16719075460294164
1105	6	0.10305530252561113
1105	7	0.26462958937082703
1105	8	0.32333201815700363
1105	9	-1.3674586441488117
1105	10	-0.09122718939612184
1105	11	0.14549476020705043
1105	12	0.21420882986772938
1105	13	0.2622931576768437
1105	14	-1.6153142974084886
1105	15	-0.22529969920604032
1105	16	-0.0086296528555323
1105	17	0.18621293765737335
1105	18	0.20264227152976133
1105	19	0.3512865756567365
1105	20	0.568313244559576
1105	21	0.7074892239435187
1105	22	0.7642131300721005
1105	23	0.9398141210482578
1105	24	0.7206952257818813
1105	25	0.3850413502103365
1106	1	0.13275450093533792
1106	2	0.08531123993908807
1106	3	0.1539802509984085
1106	4	-0.939330581604807
1106	5	-0.12515805691921855
1106	6	0.0571809081136152
1106	7	0.15969933583359364
1106	8	0.2170828395092812
1106	9	0.5551673662755405
1106	10	0.47645548747344435
1106	11	0.40333893356058326
1106	12	0.2189237420624366
1106	13	0.36999704714190074
1106	14	0.7366479685054088
1106	15	0.425002306355573
1106	16	0.39057511568753256
1106	17	0.14349460863717667
1106	18	0.29142164429165973
1106	19	0.25774187521491143
1106	20	0.3837296053256125
1106	21	0.5861089017347894
1106	22	0.6184554082963416
1106	23	0.8489132086512114
1106	24	0.455462326966547
1106	25	0.4405761358308098
1107	1	0.27549532212659855
1107	2	0.4074481019311271
1107	3	0.30407060344699843
1107	4	0.3585331395957354
1107	5	0.3573940799434254
1107	6	0.21828616374212828
1107	7	0.20068074849433515
1107	8	0.2117042252074839
1107	9	0.34997366446843614
1107	10	0.29960651093402646
1107	11	0.14487665584649534
1107	12	0.22738127399794195
1107	13	0.2922921148003965
1107	14	0.7691133342699032
1107	15	0.5313025280274672
1107	16	0.42972123302418197
1107	17	0.4971891043102943
1107	18	0.5926228709792056
1107	19	0.6558642951666727
1107	20	0.690420866106443
1107	21	0.6433379684968372
1107	22	0.6341925405661826
1107	23	0.8288965256901619
1107	24	0.7768458659187516
1107	25	0.6000885711732451
1108	1	0.5498395799413309
1108	2	0.5519410229361625
1108	3	0.636775841103173
1108	4	0.7915159991161715
1108	5	0.6051341938501054
1108	6	0.48975164631783485
1108	7	0.44633048718829116
1108	8	0.5178487059411339
1108	9	0.16583984633022197
1108	10	0.5669076350774205
1108	11	0.41712962439684276
1108	12	0.45462646061712775
1108	13	0.4684797771626279
1108	14	-0.9154573700940389
1108	15	0.09445676431262484
1108	16	0.4524385855210795
1108	17	0.5318826054212664
1108	18	0.7195096945040405
1108	19	0.6140484425737114
1108	20	0.7742618281860345
1108	21	0.6983348116670882
1108	22	0.8085703197021701
1108	23	1.1977774906313456
1108	24	0.9715440494116976
1108	25	0.8630147393815406
1109	1	0.38816356917633854
1109	2	0.3689134139358856
1109	3	0.4071088391557467
1109	4	0.5288092965581299
1109	5	0.4919428905420915
1109	6	0.34968723748198727
1109	7	0.3498762339321786
1109	8	0.3764325403742532
1109	9	0.517566543934521
1109	10	0.5138878796928879
1109	11	0.3168589550496108
1109	12	0.3009120345208913
1109	13	0.3457673717862306
1109	14	0.6882661594124428
1109	15	0.4850769051749637
1109	16	0.5841577389296688
1109	17	0.4652572014905587
1109	18	0.5380761287287896
1109	19	0.5093467570622892
1109	20	0.6113251705070155
1109	21	0.7551389306586245
1109	22	0.699222487810438
1109	23	1.1103214044274239
1109	24	0.8184843016127559
1109	25	0.6655231819301406
1110	1	0.44850869835816454
1110	2	0.315358996177153
1110	3	0.4881856132139646
1110	4	0.645528922178606
1110	5	0.6569868879236651
1110	6	0.4335636196871633
1110	7	0.5003008673607354
1110	8	0.4421466549168471
1110	9	0.5332955709350529
1110	10	0.568680189118858
1110	11	0.35612333919011974
1110	12	0.38983397767596983
1110	13	0.26620411586237935
1110	14	0.8147387677622433
1110	15	0.6198716110046476
1110	16	0.5964190876755282
1110	17	0.5872453617146718
1110	18	0.7171266753796033
1110	19	0.6708996026440488
1110	20	0.7226110958544396
1110	21	0.7456962091368027
1110	22	0.9005592386383512
1110	23	0.9719968079409185
1110	24	0.8868816075304622
1110	25	0.7902278669644645
1111	1	0.16164473153701664
1111	2	0.15650770003556858
1111	3	0.3833804962552705
1111	4	0.6095802723907084
1111	5	0.41555292894976503
1111	6	0.29523132244524675
1111	7	0.2510103965474073
1111	8	0.30330022941272716
1111	9	0.3182553662317389
1111	10	0.22900145418160633
1111	11	0.18946683187606794
1111	12	0.23833169713700642
1111	13	0.22548513799012682
1111	14	-1.6965095861788058
1111	15	-0.5062029965501296
1111	16	-0.06346738960520071
1111	17	0.2255627856643585
1111	18	0.32650847503430924
1111	19	0.27493228370467904
1111	20	0.4277284251612201
1111	21	0.4551456652034389
1111	22	0.4334947544802148
1111	23	-1.7584411066460035
1111	24	-0.26933614058872735
1111	25	0.08241266874200193
1112	1	-0.1257294197043528
1112	2	-0.24611450754324338
1112	3	-0.005397818632882689
1112	4	0.0620419947765356
1112	5	-0.8000868044050193
1112	6	-0.9354561831117891
1112	7	-0.9709948581446568
1112	8	-0.6929242597155039
1112	9	-0.6605353386752739
1112	10	-0.9124132519509374
1112	11	-0.9664644806922738
1112	12	-0.9260956696010041
1112	13	-0.7965692110450533
1112	14	-0.6155792647349898
1112	15	-0.8967963202583249
1112	16	-0.942375740031423
1112	17	-0.8958695990505348
1112	18	-0.9357769465053849
1112	19	-0.8742945044475547
1112	20	-0.960062421774893
1112	21	-1.929516596123186
1112	22	-1.8575838930793651
1112	23	-1.447055375990928
1112	24	-1.6126497450911685
1112	25	-1.8621621080432789
1113	1	0.19302993588095774
1113	2	-0.0028780556638139387
1113	3	0.05583465880557981
1113	4	0.33357309859663786
1113	5	0.3137709879046682
1113	6	0.11754077830681098
1113	7	0.09737895508860106
1113	8	0.07671561786270331
1113	9	0.45988050279252946
1113	10	0.09616139876825969
1113	11	0.07508879187328432
1113	12	0.08281965711994843
1113	13	0.02966955538896343
1113	14	0.2643407532288933
1113	15	0.09595161100074279
1113	16	0.2258426328522134
1113	17	0.2762795766753783
1113	18	0.30635651082572934
1113	19	0.07639947178613937
1113	20	-0.45498932117244806
1113	21	-0.37444687193637094
1113	22	0.23743908725813867
1113	23	-0.088724140273861
1113	24	0.19426099259447938
1113	25	-0.9631542155152175
1114	1	-0.7458447584143784
1114	2	-0.6479408622141491
1114	3	-0.4587694625878278
1114	4	-1.3097135574245584
1114	5	-0.6502866908685974
1114	6	-0.5555275102978285
1114	7	-0.37496988773599327
1114	8	-0.3434713334149393
1114	9	-0.43556145091207554
1114	10	-0.34441202533169957
1114	11	-0.4657833952293089
1114	12	-0.5244099997151963
1114	13	-0.5557474674130347
1114	14	-0.4690800154974468
1114	15	-1.1800848119006986
1114	16	-0.6931462744016699
1114	17	-0.6764527602984955
1114	18	-0.5515119503628085
1114	19	-0.6974320565246899
1114	20	-1.892440093502536
1114	21	-6.593655911322887
1114	22	-7.685414043197639
1114	23	-7.931094497007599
1114	24	-5.83666098770664
1114	25	-7.388402862296239
1115	1	-1.092789021697154
1115	2	-1.0999865839333296
1115	3	-0.8068875852779342
1115	4	-0.6495259074952813
1115	5	-0.6510392722681004
1115	6	-0.9157466586498515
1115	7	-1.1326585523762844
1115	8	-0.7818307322695333
1115	9	-2.7681845514831345
1115	10	-1.5936579338005785
1115	11	-1.1900487064537941
1115	12	-1.0646207127642668
1115	13	-1.5613697361451457
1115	14	-1.1927064436191728
1115	15	-2.06304011219963
1115	16	-1.4445844068423526
1115	17	-2.997296249672914
1115	18	-2.952741889238524
1115	19	-2.195968495416089
1115	20	-1.8257466893872234
1115	21	-1.37972029008494
1115	22	-1.0399350752391214
1115	23	-0.29438354808570466
1115	24	-1.295073814043647
1115	25	-0.8912725020840568
1116	1	-1.4680254051743837
1116	2	-0.6951928470029927
1116	3	-0.60439825703709
1116	4	-0.22363519519701924
1116	5	-0.20230455158549887
1116	6	-0.3528134247303214
1116	7	-0.6066186206162953
1116	8	-1.7365291948943096
1116	9	-0.8137790962329277
1116	10	-2.4430570316696816
1116	11	-1.0251796191268312
1116	12	-0.8064776112017142
1116	13	-0.6113034465596527
1116	14	-1.062048954624231
1116	15	-1.2163377783618188
1116	16	-0.7029003797932418
1116	17	-0.6148823705841338
1116	18	-1.1535912847318244
1116	19	-0.7763264838666402
1116	20	-1.0581832575371166
1116	21	-0.5723643927807203
1116	22	-0.053560016911696096
1116	23	0.06671456296972167
1116	24	-0.11346874196452461
1116	25	-0.043488401215256545
1201	1	0.3747382797388319
1201	2	0.40278702359369944
1201	3	0.2828914625890775
1201	4	0.4191603270377584
1201	5	0.4126158551106087
1201	6	0.23173469070564323
1201	7	0.30620822543485104
1201	8	0.474258522061632
1201	9	0.6535794330882183
1201	10	0.6916554054259336
1201	11	0.6267326741873305
1201	12	0.5085213617879134
1201	13	0.45542655241334273
1201	14	1.0716236344689207
1201	15	0.7921124629856138
1201	16	0.7696342818068445
1201	17	0.744448018652804
1201	18	0.5695272322652016
1201	19	0.6367406826117374
1201	20	0.7612996427401768
1201	21	0.7806468016323436
1201	22	0.876120069357178
1201	23	0.561671428534062
1201	24	0.7652541706368936
1201	25	0.6501477290096602
1202	1	-0.32871149539994704
1202	2	-0.027438364643249002
1202	3	-0.1959584074130305
1202	4	-0.19434803498880626
1202	5	-0.21757132428648163
1202	6	-0.31030908696282467
1202	7	-0.3931628658447508
1202	8	-0.15727167609731652
1202	9	0.05208050469246217
1202	10	0.16337515456513785
1202	11	-6.336387913776775E-4
1202	12	-0.09064331392004732
1202	13	0.025202481722594428
1202	14	0.19112990598883783
1202	15	0.2246093008747323
1202	16	0.0906667110106909
1202	17	0.037058780370180955
1202	18	-0.04066618351724571
1202	19	-0.06491020277930915
1202	20	0.1871485816763041
1202	21	0.10351545105964988
1202	22	0.03453787204145442
1202	23	0.3066234698523118
1202	24	-0.07600127522074018
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2211	2	0.055811130930116803
2211	3	0.16071772508676574
2211	4	0.231437546489893
2211	5	0.3163818184394529
2211	6	0.14787070493019172
2211	7	0.20505452003322944
2211	8	0.21462941028004678
2211	9	0.27204776896401484
2211	10	0.2444250840014064
2211	11	0.04694407331182049
2211	12	0.17385615165173363
2211	13	-0.009866619925972486
2211	14	0.2962261050681505
2211	15	0.27060042920631133
2211	16	0.11401984405611643
2211	17	0.11442711541826611
2211	18	0.1918311923441962
2211	19	0.21422848574344755
2211	20	0.3274743408763854
2211	21	0.2554017206546888
2211	22	0.2433545475619283
2211	23	0.38234996821142175
2211	24	0.3932827529759493
2211	25	0.5050391555012794
2212	1	-0.2310267550575027
2212	2	-0.18122507704536517
2212	3	-0.1104872176270888
2212	4	-0.4180102600952438
2212	5	-0.06983477199887744
2212	6	-0.4225984818027726
2212	7	-0.3797036733832968
2212	8	-0.06731443178232865
2212	9	-0.008902447184809148
2212	10	-0.3677929686987724
2212	11	-0.2821154604231282
2212	12	-0.390825197240936
2212	13	-0.5284216360447402
2212	14	-0.24902919263927004
2212	15	-0.44780155312128755
2212	16	-0.3566671615249888
2212	17	-0.283944974263008
2212	18	-0.2547505633421281
2212	19	-0.4265866592754719
2212	20	-0.29985473194885515
2212	21	-0.2161709112219441
2212	22	-0.2563926440679012
2212	23	0.1288774150980032
2212	24	-0.16921030392872183
2212	25	-0.8945567336663984
2213	1	-0.09559827918064912
2213	2	-1.328714055979514
2213	3	-0.2113375487740221
2213	4	0.0939995730701284
2213	5	0.14634581996537577
2213	6	0.0803450356962685
2213	7	0.04583407845529308
2213	8	0.14647369597536652
2213	9	0.03601391008621846
2213	10	-0.4633527898743779
2213	11	-0.18635616602007588
2213	12	-0.06697035623071201
2213	13	-0.3429186273316631
2213	14	-0.09566726717172003
2213	15	-0.1793588298230091
2213	16	-0.13025421482835497
2213	17	0.06777704420007069
2213	18	0.19256154220515498
2213	19	0.1426511489558493
2213	20	0.20853412289021378
2213	21	-0.479996844645882
2213	22	-0.8359455403258877
2213	23	0.04607829367409266
2213	24	0.037613944320526116
2213	25	-0.41478465976278045
2214	1	-0.1836337137797237
2214	2	-0.38961095115403666
2214	3	-0.4083490836585426
2214	4	-0.5359220181663318
2214	5	-0.20784862175865015
2214	6	-0.15701598774593606
2214	7	-0.31432984114650253
2214	8	0.0826653790777172
2214	9	0.14263724706768954
2214	10	-1.7331890954515998
2214	11	-0.6794783767490316
2214	12	-0.4603068087104347
2214	13	-0.20044160810137157
2214	14	-0.6694830042541255
2214	15	-0.36100207548902574
2214	16	-0.538384124535142
2214	17	-0.39637713694536814
2214	18	-0.41294563957578845
2214	19	-1.2407227404290708
2214	20	-2.179679328231998
2214	21	-1.7083535668114322
2214	22	-1.8200705983184164
2214	23	-2.2585203801287292
2214	24	-1.3972102230353798
2214	25	-1.6477809632526288
2215	1	-0.12077275098969409
2215	2	-0.2731393933636852
2215	3	-0.8018236222710513
2215	4	-0.2143390921892916
2215	5	-0.06316799049679389
2215	6	-0.08567766402691035
2215	7	-0.15595520822940045
2215	8	-0.03880619800975893
2215	9	-0.08108875460334986
2215	10	-0.17943808022096164
2215	11	-0.5867662899195167
2215	12	-0.33618474928911724
2215	13	-0.3333473141889485
2215	14	0.021822304330662234
2215	15	-0.3927894190353527
2215	16	-0.45290356737423565
2215	17	-0.12021842770930391
2215	18	-0.19647118272923336
2215	19	-2.1568563203227953
2215	20	-2.349214474890587
2215	21	-3.3172265008187765
2215	22	-2.504060187924253
2215	23	-3.4068120416981102
2215	24	-2.3625270095491544
2215	25	-1.5478390072046508
2216	1	-1.4716191792535938
2216	2	-1.176618454836369
2216	3	-0.7875266281266278
2216	4	-0.9412899245022359
2216	5	-1.7742395421612684
2216	6	-1.4808677652118476
2216	7	-0.9964994840893908
2216	8	-4.838674363641417
2216	9	-3.820443392023435
2216	10	-1.9898614462410862
2216	11	-1.0566077163801708
2216	12	-1.1257205904446934
2216	13	-1.7910680185572332
2216	14	-3.221496066304354
2216	15	-1.874705430127527
2216	16	-2.655370002942476
2216	17	-2.4760189686139356
2216	18	-2.5031262373735714
2216	19	-1.5290244223898242
2216	20	-0.96810205502873
2216	21	-0.7023699643396881
2216	22	-0.663778256061974
2216	23	-0.8578012448470602
2216	24	-0.5137639160461092
2216	25	-0.17702136232103527
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	87.0
8	437.0
9	1096.0
10	2155.0
11	3780.0
12	5892.0
13	8652.0
14	10988.0
15	13492.0
16	14868.0
17	16228.0
18	17078.0
19	17221.0
20	17917.0
21	18930.0
22	20017.0
23	21570.0
24	22952.0
25	24916.0
26	26715.0
27	30023.0
28	34100.0
29	40240.0
30	47816.0
31	59376.0
32	78726.0
33	110516.0
34	159522.0
35	248758.0
36	430379.0
37	1352856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.02003707392555	19.83300979710865	13.009482032799397	24.1374710961664
2	16.027548924858966	19.617302925813153	39.74134729447954	24.613800854848346
3	19.62173376864999	23.711666073339448	28.62564062712762	28.04095953088294
4	12.81904891254885	16.06141477163736	36.41438658737904	34.705149728434755
5	13.71204725272148	37.68281323672671	34.46072173533478	14.144417775217033
6	33.38299947813797	36.90159558177816	16.202608470125238	13.512796469958632
7	28.34498126380016	31.961573553802307	20.992208386719923	18.7012367956776
8	25.64925917694883	37.24130690040756	18.994258555731907	18.11517536691171
9	24.643805843999033	17.157936366277138	20.72925744324167	37.469000346482154
10	15.220361571607361	30.21281599717776	32.58869199777132	21.978130433443553
11	34.447386242534435	24.43413940156477	21.895645691626843	19.22282866427395
12	21.628900458766328	26.357469547407486	30.23563397810745	21.77799601571873
13	27.70507151144376	22.585156628698723	25.805262780275335	23.904509079582184
14	21.06384021222907	22.287727182050123	26.888240255626833	29.760192350093973
15	23.278108923577243	30.416333741767875	23.02240860183442	23.283148732820464
16	22.844049316071473	29.964444746211644	24.858018726443635	22.333487211273244
17	21.466595082534077	29.443897686021913	26.946071109082737	22.14343612236127
18	21.862427810806828	28.895066548277732	27.734509662966023	21.507995977949417
19	22.347659343778403	28.227307136779974	27.95999155845542	21.465041960986206
20	22.82210972265727	27.82512831191739	27.836222721140096	21.51653924428524
21	23.074807972053886	27.482282359279044	27.49811466018523	21.94479500848184
22	22.584083393549477	27.317264875955914	27.87607535289929	22.22257637759532
23	22.631828189897885	27.489644360037914	27.81872008283111	22.059807367233095
24	22.517048586502874	27.424801602925864	27.705278630863322	22.35287117970794
25	22.673019222526296	27.427649823250412	27.47745820130021	22.421872752923086
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2298.0
1	2298.0
2	2452.5
3	2607.0
4	2607.0
5	2607.0
6	3583.0
7	4559.0
8	4559.0
9	4559.0
10	7132.5
11	9706.0
12	9706.0
13	9706.0
14	16186.5
15	22667.0
16	22667.0
17	22667.0
18	34776.5
19	46886.0
20	46886.0
21	46886.0
22	66331.5
23	85777.0
24	85777.0
25	85777.0
26	112872.0
27	139967.0
28	139967.0
29	139967.0
30	168990.0
31	198013.0
32	198013.0
33	198013.0
34	224951.5
35	251890.0
36	251890.0
37	251890.0
38	273576.5
39	295263.0
40	295263.0
41	295263.0
42	305703.5
43	316144.0
44	316144.0
45	316144.0
46	322392.0
47	328640.0
48	328640.0
49	328640.0
50	322670.5
51	316701.0
52	316701.0
53	316701.0
54	298777.5
55	280854.0
56	280854.0
57	280854.0
58	251730.0
59	222606.0
60	222606.0
61	222606.0
62	190870.0
63	159134.0
64	159134.0
65	159134.0
66	126406.5
67	93679.0
68	93679.0
69	93679.0
70	70541.5
71	47404.0
72	47404.0
73	47404.0
74	34177.0
75	20950.0
76	20950.0
77	20950.0
78	14262.5
79	7575.0
80	7575.0
81	7575.0
82	5155.5
83	2736.0
84	2736.0
85	2736.0
86	1815.0
87	894.0
88	894.0
89	894.0
90	581.0
91	268.0
92	268.0
93	268.0
94	165.0
95	62.0
96	62.0
97	62.0
98	45.0
99	28.0
100	28.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029853274480735013
2	0.014944136228925967
3	0.018793913711787458
4	0.018758915734670534
5	0.0174639905813444
6	0.00808453271400913
7	1.749898855846132E-4
8	4.5497370251999434E-4
9	6.299635881046076E-4
10	1.3999190846769057E-4
11	8.049534736892207E-4
12	0.002799838169353811
13	0.002554852329535353
14	6.299635881046076E-4
15	0.0019948846956645908
16	0.002274868512599972
17	0.002239870535483049
18	0.002064880649898436
19	3.84977748286149E-4
20	4.5497370251999434E-4
21	0.08347017542386051
22	0.08102031702567591
23	0.08196526240783282
24	0.0011549332448584472
25	0.2233570899602003
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2857308.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.32190751510109
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.76640014607126	59.03057536575944
2	11.051347567499722	15.764063782529053
3	3.0699358556188017	6.568610435152107
4	1.2192942839927985	3.4784957662650315
5	0.5888485287552325	2.0998900154142017
6	0.3364878019702437	1.4399371135248822
7	0.2084062756438317	1.0404753181925221
8	0.1533224161092768	0.8748197745390149
9	0.10793922813622128	0.6928588481744674
>10	0.4654063574181497	5.74848289548722
>50	0.02359496399848423	1.1415078160442338
>100	0.007687165673071074	0.952536112841989
>500	7.877979930794051E-4	0.35808840332684744
>1k	5.4161111991423E-4	0.8096583527489869
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4403	0.1540960932458104	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3558	0.12452280258201077	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.499797711692264E-5	0.0	0.0	0.0	0.0
7	3.499797711692264E-5	0.0	0.0	0.0	0.0
8	3.499797711692264E-5	0.0	0.0	0.0	0.0
9	3.499797711692264E-5	0.0	0.0	3.499797711692264E-5	0.0
10	3.499797711692264E-5	0.0	0.0	6.999595423384528E-5	0.0
11	3.499797711692264E-5	0.0	0.0	6.999595423384528E-5	0.0
12	3.499797711692264E-5	3.499797711692264E-5	0.0	1.3999190846769057E-4	0.0
13	3.499797711692264E-5	3.499797711692264E-5	0.0	2.0998786270153585E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTCGC	50	0.0015047361	13.294536	6
TACGTCG	60	4.0996246E-4	12.664566	5
CGTAGCA	110	3.8216967E-8	12.090174	2
AGGCGTC	70	0.0014980622	10.851923	7
CTAGGAC	290	0.0	10.154555	3
GTCTAGG	320	0.0	10.095257	1
CAGAACG	105	4.111808E-5	9.951427	4
GTCTAGC	185	6.475602E-10	9.758213	1
CGATTTC	120	1.6899901E-5	9.505412	16
GTCCTAT	270	0.0	9.501418	1
TGAACCG	90	0.0011158059	9.498425	5
TCGTTTG	90	0.0011188464	9.495599	13
TGTGCGA	80	0.0045353584	9.495433	10
TATACTG	895	0.0	9.445361	5
AGGACTG	660	0.0	9.354509	5
TATTAGG	275	0.0	9.326705	2
TTAGACT	225	2.7284841E-11	9.287999	4
ACGTAGC	115	1.1093497E-4	9.088313	1
TAGGACA	315	0.0	9.046752	4
ATCCCGT	95	0.0018312659	8.996303	6
>>END_MODULE