FastQCFastQC Report
Wed 25 May 2016
SRR1294717_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294717_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2857308
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT122240.42781527227726235No Hit
GGTATCAACGCAGAGTACTTTTTTT98490.3446950766245711No Hit
TATCAACGCAGAGTACTTTTTTTTT83470.2921281149949533No Hit
ACGCAGAGTACTTTTTTTTTTTTTT48980.1714200919186871No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA551.1357506E-515.5356349
ACCGTCC653.396719E-614.6061518
CGCACGA400.005302721614.24099712
ATACCGT759.738578E-713.9259926
GTCCGGA604.0237358E-412.6926461
GTCGTAC701.0708375E-412.2393381
TATACCG550.003033125312.1101855
TCGCGCG658.069705E-411.684929
TGGACCG907.335342E-611.62962155
ACGTCGC600.00590080211.0774946
GCGACGT803.7948551E-410.6807488
GTGCTAG2500.010.2810431
CTATCGG1951.4551915E-1110.22430512
TATCGGT1951.4551915E-1110.22430513
ATCGGTC1803.9835868E-1010.02144214
GTCCTAG3300.09.8079541
TTAGGCT1856.311893E-109.772314
GGTATCA29700.09.6797461
CTAGCAC3150.09.6661793
TATACTG9400.09.6163695
Produced by FastQC (version 0.11.5)