FastQCFastQC Report
Wed 25 May 2016
SRR1294716_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294716_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323532
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC95950.4129489070948883No Hit
CCCATGTACTCTGCGTTGATACCAC80720.3474021446659654No Hit
GAGTACATGGGAAGCAGTGGTATCA46060.19823269057624343No Hit
GTATCAACGCAGAGTACTTTTTTTT40890.17598208245033853No Hit
CATGTACTCTGCGTTGATACCACTG31020.13350364875542922No Hit
GGTATCAACGCAGAGTACTTTTTTT30930.13311630741474617No Hit
GTATCAACGCAGAGTACATGGGAAG27490.11831126061530461No Hit
GCGTTGATACCACTGCTTCCCATGT27140.11680493317931494No Hit
ACGCAGAGTACATGGGAAGCAGTGG25930.11159734404346486No Hit
TATCAACGCAGAGTACTTTTTTTTT24730.10643279283435736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC2000.010.9281211
GTCTTAG2350.010.9180111
TGCACCG803.777997E-410.685725
GTATAGG2500.010.643041
GTAGGAC1553.6652636E-910.4191893
GTCCTAG2950.010.3080281
TCTAGAC1856.002665E-1110.2701073
TAGGCTG3650.09.8887635
GTATTAG3400.09.7822051
CTAGACA2151.07320375E-109.2781244
GTGTTAC2054.2018655E-109.270941
TAGGACT3200.09.2021874
TAAGACT3150.09.0466974
GTCCTAT1901.053013E-89.002571
CCTACAC2850.08.9998573
CTTAGAC1901.0584699E-88.9998573
CGAGTGC850.00746303058.93737910
GTCTCGC1606.714854E-78.9087941
TCGCTCT1606.752507E-78.9053424
CCATAAG1502.6919515E-68.86921
Produced by FastQC (version 0.11.5)