FastQCFastQC Report
Wed 25 May 2016
SRR1294716_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294716_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323532
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT107260.4616248022407266No Hit
GTACATGGGAAGCAGTGGTATCAAC98360.4233210474398459No Hit
CCCATGTACTCTGCGTTGATACCAC90340.38880463019231065No Hit
GGTATCAACGCAGAGTACTTTTTTT84870.36526288426412895No Hit
TATCAACGCAGAGTACTTTTTTTTT68770.29597182220860313No Hit
GAGTACATGGGAAGCAGTGGTATCA51810.2229794984532169No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45080.19401497375547228No Hit
CATGTACTCTGCGTTGATACCACTG36010.154979574199968No Hit
ACGCAGAGTACATGGGAAGCAGTGG32400.13944288264590288No Hit
GCGTTGATACCACTGCTTCCCATGT30680.1320403592461821No Hit
GTATCAACGCAGAGTACATGGGAAG29130.1253694806010849No Hit
GGTATCAACGCAGAGTACATGGGAA23610.10161254503919033No Hit
TATCAACGCAGAGTACATGGGAAGC23380.10062267272411139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCA602.5256795E-514.2759222
TATACCG400.00521685714.2759225
TATCGCG400.005304846414.2399777
GACTGCG550.00308544712.0824047
GTTATAC2000.011.8966023
AGAACCG657.904595E-411.7135775
TGTACCG600.00579816711.1034955
GCGCGCC600.005908000711.07553811
CGTTCCG600.005908000711.07553810
GTCTTAG1900.011.0271461
ATCGCGC700.001500665110.8495068
CGCGCCT803.797546E-410.67975312
TAGACAG2900.010.5018275
CCTACAC2400.09.9138353
TAGTACT1606.454502E-89.5172824
CTTACAC3050.09.361263
GCTACAC2054.0927262E-109.2851523
CTACACT4450.09.1964734
TACACTG5500.09.1711985
GTAGGGC1356.6932134E-69.1647893
Produced by FastQC (version 0.11.5)