FastQCFastQC Report
Wed 25 May 2016
SRR1294715_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294715_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4898896
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1135142.317134309444414No Hit
CCCATGTACTCTGCGTTGATACCAC1123892.29416995176056No Hit
GAGTACATGGGAAGCAGTGGTATCA528981.0797943046759924No Hit
CATGTACTCTGCGTTGATACCACTG446690.9118176830044974No Hit
GCGTTGATACCACTGCTTCCCATGT368920.7530676299313152No Hit
ACGCAGAGTACATGGGAAGCAGTGG302310.6170982196805157No Hit
GTATCAACGCAGAGTACATGGGAAG292530.5971345380673523No Hit
GCTTCCCATGTACTCTGCGTTGATA261900.5346102468801134No Hit
ACTCTGCGTTGATACCACTGCTTCC252450.5153201864256763No Hit
TATCAACGCAGAGTACATGGGAAGC237650.48510929809491776No Hit
GGTATCAACGCAGAGTACATGGGAA197150.4024376104330445No Hit
CAGTGGTATCAACGCAGAGTACATG158160.3228482498914041No Hit
GTGGTATCAACGCAGAGTACATGGG154640.31566295753165613No Hit
ATACCACTGCTTCCCATGTACTCTG132540.27055075265937467No Hit
GCAGTGGTATCAACGCAGAGTACAT124690.25452673418664123No Hit
GTACTCTGCGTTGATACCACTGCTT116290.23738001378269719No Hit
ACATGGGAAGCAGTGGTATCAACGC112670.22999059379909267No Hit
GTACATGGTAAGCAGTGGTATCAAC110350.22525483292562243No Hit
CATGGGAAGCAGTGGTATCAACGCA109980.22449956071735347No Hit
CTGCTTCCCATGTACTCTGCGTTGA109230.2229686035384299No Hit
GGGAAGCAGTGGTATCAACGCAGAG101480.20714871268955293No Hit
TACCACTGCTTCCCATGTACTCTGC96740.1974730633187559No Hit
CTTCCCATGTACTCTGCGTTGATAC93220.1902877709590079No Hit
GATACCACTGCTTCCCATGTACTCT92690.1892058945525686No Hit
GTTGATACCACTGCTTCCCATGTAC91820.18742998422501722No Hit
GCAGAGTACATGGGAAGCAGTGGTA88740.18114285341023773No Hit
AAGCAGTGGTATCAACGCAGAGTAC88400.18044881948912572No Hit
CCACTGCTTCCCATGTACTCTGCGT87270.17814217733954754No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA84720.17293692293120735No Hit
CCATGTACTCTGCGTTGATACCACT84300.17207958691101016No Hit
ACCATGTACTCTGCGTTGATACCAC82970.16936468951371902No Hit
TTCCCATGTACTCTGCGTTGATACC74420.15191177767399022No Hit
ATGGGAAGCAGTGGTATCAACGCAG73700.15044205878222358No Hit
TGATACCACTGCTTCCCATGTACTC66050.13482629555720307No Hit
GTACATGGGTAAGCAGTGGTATCAA63310.1292331986635356No Hit
GGTAAGCAGTGGTATCAACGCAGAG50270.10261495651265101No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC4500.010.34326653
TAACGCT750.00265383910.13124754
GGACCGT1502.4638211E-810.1294896
TACGACA951.6488838E-49.9979424
CGCGCGC951.6517735E-49.99620710
CTATACT5500.09.8434294
CTTAACG800.00452034189.4994992
TAGACCG800.00452834279.4974635
TAGTCCG1002.7649623E-49.4974635
TACACCG1401.0638578E-69.4974635
AACGCTA900.001117149.4974625
GTCTTAG4050.09.38452151
GTATAGG4600.09.2952671
CCTACAC5150.09.222253
GTATTAG4450.09.1815411
GTGTAGG5800.09.1741791
CTAGACT3850.09.1279914
CCGCGCG950.00183139678.9964949
GTACAAA11200.08.9928021
ACGTCGC850.00746270558.9377856
Produced by FastQC (version 0.11.5)