FastQCFastQC Report
Wed 25 May 2016
SRR1294715_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294715_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4898896
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1237202.525466962352334No Hit
GTACATGGGAAGCAGTGGTATCAAC1120642.2875358039852243No Hit
GAGTACATGGGAAGCAGTGGTATCA596281.2171721955314014No Hit
CATGTACTCTGCGTTGATACCACTG508631.038254333221199No Hit
GCGTTGATACCACTGCTTCCCATGT396650.8096722200267162No Hit
ACGCAGAGTACATGGGAAGCAGTGG369370.7539862042386692No Hit
GTATCAACGCAGAGTACATGGGAAG312170.6372252033927643No Hit
ACTCTGCGTTGATACCACTGCTTCC297570.6074225703097187No Hit
GCTTCCCATGTACTCTGCGTTGATA288350.5886020033901516No Hit
TATCAACGCAGAGTACATGGGAAGC279330.5701896917182974No Hit
GGTATCAACGCAGAGTACATGGGAA218570.4461617474631019No Hit
CAGTGGTATCAACGCAGAGTACATG193180.3943337437659423No Hit
GTGGTATCAACGCAGAGTACATGGG164720.336239022016389No Hit
ATACCACTGCTTCCCATGTACTCTG146940.2999451304947074No Hit
ACATGGGAAGCAGTGGTATCAACGC144910.2958013397304209No Hit
CATGGGAAGCAGTGGTATCAACGCA143910.2937600634918561No Hit
GCAGTGGTATCAACGCAGAGTACAT141960.28977957482665484No Hit
GTATCAACGCAGAGTACTTTTTTTT140210.2862073414091665No Hit
GGGAAGCAGTGGTATCAACGCAGAG122750.25056665828382557No Hit
CTGCTTCCCATGTACTCTGCGTTGA119430.24378962117179054No Hit
GTACTCTGCGTTGATACCACTGCTT117550.23995202184328876No Hit
CTTCCCATGTACTCTGCGTTGATAC112710.23007224484863528No Hit
TACCACTGCTTCCCATGTACTCTGC111010.22660207524307519No Hit
AAGCAGTGGTATCAACGCAGAGTAC109960.22445873519258216No Hit
GTACATGGTAAGCAGTGGTATCAAC108960.22241745895401738No Hit
GATACCACTGCTTCCCATGTACTCT108250.2209681528246364No Hit
GGTATCAACGCAGAGTACTTTTTTT106660.2177225236053184No Hit
TATCAACGCAGAGTACTTTTTTTTT103770.21182323527586625No Hit
GCAGAGTACATGGGAAGCAGTGGTA103230.21072094610704123No Hit
CCATGTACTCTGCGTTGATACCACT102290.20880214644279038No Hit
CCACTGCTTCCCATGTACTCTGCGT102050.20831224014553484No Hit
GTTGATACCACTGCTTCCCATGTAC99030.2021475859050692No Hit
ACCATGTACTCTGCGTTGATACCAC90990.18573572494700846No Hit
ATGGGAAGCAGTGGTATCAACGCAG88950.18157152142033633No Hit
TTCCCATGTACTCTGCGTTGATACC76090.15532070899239336No Hit
TGATACCACTGCTTCCCATGTACTC70380.14366502167018855No Hit
GTACATGGGTAAGCAGTGGTATCAA68200.13921503947011735No Hit
ACGCAGAGTACTTTTTTTTTTTTTT67060.1368879845581535No Hit
GGTAAGCAGTGGTATCAACGCAGAG62240.1270490330882713No Hit
ATCAACGCAGAGTACATGGGAAGCA57470.11731214543031737No Hit
GTACTTTTTTTTTTTTTTTTTTTTT56670.11567912443946554No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA56200.1147197246073401No Hit
CCCCATGTACTCTGCGTTGATACCA53870.10996355097148418No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGC803.7200082E-410.7041174
TAGGACG1002.3604493E-510.4662474
TATACTG8250.09.6877665
AGTACTC13350.09.5504065
TAAGGGT2303.6379788E-129.51477054
TACACCG1201.6711962E-59.514775
ACGCAAA2550.09.332121
GTATTAG3950.09.157291
GTAGGAC2201.6370905E-109.082283
TAGAACA5300.08.9761984
GTTCTAT3750.08.8841781
CTACACT7500.08.8804524
GTCCTAC2800.08.838851
ACACCGT1401.0935761E-58.8167466
ACTGTCC6050.08.7882738
CTAGGAC3900.08.7828653
TACACTG10300.08.6833825
CCAGGAC8900.08.659513
TTTAGAC2751.8189894E-128.6497913
TCTTACG1000.00286611968.56329252
Produced by FastQC (version 0.11.5)