FastQCFastQC Report
Wed 25 May 2016
SRR1294714_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294714_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3556943
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1387973.902142935661325No Hit
CCCATGTACTCTGCGTTGATACCAC1322163.717124508320769No Hit
GAGTACATGGGAAGCAGTGGTATCA655621.8432119941196696No Hit
CATGTACTCTGCGTTGATACCACTG522501.4689580350317675No Hit
GCGTTGATACCACTGCTTCCCATGT422471.1877333991576473No Hit
GCTTCCCATGTACTCTGCGTTGATA389001.0936357428274786No Hit
ACGCAGAGTACATGGGAAGCAGTGG360511.0135388731278516No Hit
GTATCAACGCAGAGTACATGGGAAG358971.0092093126035475No Hit
ACTCTGCGTTGATACCACTGCTTCC282590.7944743562098128No Hit
TATCAACGCAGAGTACATGGGAAGC266990.7506164703791992No Hit
GGTATCAACGCAGAGTACATGGGAA234350.658852278487454No Hit
CAGTGGTATCAACGCAGAGTACATG200040.5623930436894827No Hit
GTGGTATCAACGCAGAGTACATGGG188400.5296683134927942No Hit
GCAGTGGTATCAACGCAGAGTACAT160440.4510614873502331No Hit
GTACATGGTAAGCAGTGGTATCAAC151900.42705210626091006No Hit
ATACCACTGCTTCCCATGTACTCTG145090.4079064522540845No Hit
CATGGGAAGCAGTGGTATCAACGCA140940.3962391300619661No Hit
GTACTCTGCGTTGATACCACTGCTT140170.39407434979981404No Hit
ACATGGGAAGCAGTGGTATCAACGC133080.3741415029703878No Hit
CTGCTTCCCATGTACTCTGCGTTGA132870.3735511083534372No Hit
GGGAAGCAGTGGTATCAACGCAGAG130650.3673097938313884No Hit
TACCACTGCTTCCCATGTACTCTGC112370.3159173481273105No Hit
AAGCAGTGGTATCAACGCAGAGTAC111860.3144835326290019No Hit
GCAGAGTACATGGGAAGCAGTGGTA110390.31035077031034797No Hit
GATACCACTGCTTCCCATGTACTCT109790.308663928547632No Hit
ACCATGTACTCTGCGTTGATACCAC108340.3045873942877353No Hit
CTTCCCATGTACTCTGCGTTGATAC108290.3044468241408423No Hit
GTTGATACCACTGCTTCCCATGTAC104550.2939321771532465No Hit
CCATGTACTCTGCGTTGATACCACT101450.285216828045881No Hit
CCACTGCTTCCCATGTACTCTGCGT101080.2841766089588728No Hit
GTACATGGGTAAGCAGTGGTATCAA97870.27515200552834274No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA96020.269950910093302No Hit
ATGGGAAGCAGTGGTATCAACGCAG94520.2657338056865123No Hit
TTCCCATGTACTCTGCGTTGATACC79720.22412504220618662No Hit
TGATACCACTGCTTCCCATGTACTC71940.20225232734963705No Hit
GGTAAGCAGTGGTATCAACGCAGAG71400.20073416976319272No Hit
CCCCATGTACTCTGCGTTGATACCA66520.1870145234264367No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA61340.1724514562083227No Hit
GCTTACCATGTACTCTGCGTTGATA60010.16871229030096913No Hit
ATCAACGCAGAGTACATGGGAAGCA57500.16165566892694092No Hit
GAGTACATGGTAAGCAGTGGTATCA51420.14456233906475308No Hit
AAAAAGTACTCTGCGTTGATACCAC45760.12864979843646637No Hit
GGAAGCAGTGGTATCAACGCAGAGT43550.1224365979437961No Hit
ACTCTGCGTTGATACCACTGCTTAC38250.10753616237313895No Hit
GCGTTGATACCACTGCTTACCATGT37340.1049777856996865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAA9000.013.6203671
CGCAAAA3650.013.0148542
TAACCCG550.003073237812.0890935
ACATCGG658.038007E-411.6905515
TTAGGAC1451.2187229E-911.137983
CACGGAC600.005877988411.0833823
TCGTCAC600.005897707311.07839614
ACGCAAA4550.011.0689411
GTATCGA1158.784773E-710.74310718
CCGGGGT1659.313226E-1010.3629534
ACCGGAG951.6390409E-410.00370318
GCAGGAC1901.0004442E-1010.0000453
CTAGACA3250.09.9378074
GAACAAA17300.09.8870781
TACCGGG1252.5695572E-69.8808782
GTCTTAG1951.6370905E-109.7462381
CGGTGGT2054.1836756E-119.72737213
CCTAGAC2251.8189894E-129.7111543
GTGTAAC2002.6375346E-109.5025821
CAGGACC2800.09.4993744
Produced by FastQC (version 0.11.5)