##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294714_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3556943
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.80794322540451	33.0	33.0	33.0	27.0	33.0
2	30.79097950121776	33.0	33.0	33.0	27.0	33.0
3	30.796176660688687	33.0	33.0	33.0	27.0	33.0
4	33.995413477247176	37.0	33.0	37.0	27.0	37.0
5	34.54562752340985	37.0	37.0	37.0	27.0	37.0
6	34.62598529130211	37.0	37.0	37.0	27.0	37.0
7	34.67571760357138	37.0	37.0	37.0	27.0	37.0
8	34.573367636197716	37.0	37.0	37.0	27.0	37.0
9	34.232340805011496	37.0	37.0	37.0	27.0	37.0
10	34.36917993906565	37.0	37.0	37.0	27.0	37.0
11	34.63953850258495	37.0	37.0	37.0	27.0	37.0
12	34.59110393391179	37.0	37.0	37.0	27.0	37.0
13	34.42023670325895	37.0	37.0	37.0	27.0	37.0
14	35.647449228171496	40.0	37.0	40.0	27.0	40.0
15	35.85447700455138	40.0	37.0	40.0	27.0	40.0
16	35.840725027080836	37.0	37.0	40.0	27.0	40.0
17	35.858975811532545	40.0	37.0	40.0	27.0	40.0
18	35.83832465125249	40.0	37.0	40.0	27.0	40.0
19	35.80019274978542	40.0	37.0	40.0	27.0	40.0
20	35.728277062634966	37.0	37.0	40.0	27.0	40.0
21	35.63605911030905	37.0	37.0	40.0	27.0	40.0
22	35.584011045439865	37.0	37.0	40.0	27.0	40.0
23	35.25644015099483	37.0	33.0	40.0	27.0	40.0
24	35.60170967035457	37.0	37.0	40.0	27.0	40.0
25	34.5054767534931	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5741928656103106
1101	2	0.7224376613965582
1101	3	0.5366555684974124
1101	4	0.6754628698921152
1101	5	0.6447770090411638
1101	6	0.5474929754955298
1101	7	0.5523026428625073
1101	8	0.668533870640907
1101	9	0.8902241877509667
1101	10	0.7719054737090545
1101	11	0.5033721245094469
1101	12	0.4841997255841548
1101	13	0.6244924078510152
1101	14	1.2880329918891675
1101	15	1.101151297227588
1101	16	1.0473544008180227
1101	17	1.0988859427505986
1101	18	1.0364661185818136
1101	19	1.0700061903428306
1101	20	1.227055738322619
1101	21	1.1813633057746813
1101	22	1.2453054991988068
1101	23	1.520117122845555
1101	24	1.311180132717901
1101	25	1.276481418422101
1102	1	0.36911027582901923
1102	2	0.5961931245179066
1102	3	0.39057867800894996
1102	4	0.34570690546638616
1102	5	0.28404206783671526
1102	6	0.25042604161039606
1102	7	0.3126300507354003
1102	8	0.3660068888227883
1102	9	0.4804092044458912
1102	10	0.5742643713434958
1102	11	0.4130022980695145
1102	12	0.3653707399397348
1102	13	0.44162321334055576
1102	14	0.9877601070339068
1102	15	0.835279307968328
1102	16	0.5774230099184194
1102	17	0.7113551107776317
1102	18	0.7238658784197156
1102	19	0.7201042102252089
1102	20	0.8036093069234553
1102	21	0.79884544054363
1102	22	0.8986167923016666
1102	23	1.225750596351169
1102	24	1.012378056728899
1102	25	0.8533209787063285
1103	1	0.3920700216674504
1103	2	0.5321672445070504
1103	3	0.23661409516196485
1103	4	0.12088273274236627
1103	5	0.2888779737853753
1103	6	0.21380868541083942
1103	7	0.25490828860733217
1103	8	0.5036416821302083
1103	9	0.6660928834011344
1103	10	0.6851361582991586
1103	11	0.45772356364569333
1103	12	0.3379795156009351
1103	13	0.4881864735192991
1103	14	1.0979075973937285
1103	15	0.793622026107073
1103	16	0.5790745582402153
1103	17	0.6601713706088645
1103	18	0.7038079194837152
1103	19	0.7070348579706902
1103	20	0.7713819865318996
1103	21	0.7606403729768303
1103	22	0.7105760887340224
1103	23	1.1541812718518472
1103	24	0.9577841532911364
1103	25	0.8154954349515862
1104	1	0.3039575805314385
1104	2	0.5716480471397958
1104	3	0.35196881670889013
1104	4	0.26442816428473037
1104	5	0.2666043029788554
1104	6	0.1344912277828314
1104	7	0.17380298541813488
1104	8	0.32208252256130976
1104	9	0.5269796891344853
1104	10	0.5901241746396266
1104	11	0.46273800300375
1104	12	0.3179981535316969
1104	13	0.31787885436007457
1104	14	0.8502291655177103
1104	15	0.6831064861631546
1104	16	0.6509510627287725
1104	17	0.624722989167978
1104	18	0.5980900232634809
1104	19	0.7174524685387027
1104	20	0.7957839791558499
1104	21	0.7824824305088427
1104	22	0.8051731041272987
1104	23	1.235240521363508
1104	24	0.9828380629647171
1104	25	0.8795711033083791
1105	1	0.33666261123765295
1105	2	0.3667165466031719
1105	3	0.3476112278768362
1105	4	0.4154352619252464
1105	5	0.42083188208417965
1105	6	0.30962701155431915
1105	7	0.3432737135256332
1105	8	0.44837670863312695
1105	9	-1.6252731758066332
1105	10	-0.04767979018615165
1105	11	0.24058977307991825
1105	12	0.32489961367469533
1105	13	0.37309335166524704
1105	14	-1.4592557933471326
1105	15	-0.07467351569111713
1105	16	0.1717164295054232
1105	17	0.41987414479068974
1105	18	0.32255172959747824
1105	19	0.3712453530503055
1105	20	0.5183106901703454
1105	21	0.7666750252118675
1105	22	0.7487287855770433
1105	23	1.0104905973907137
1105	24	0.827950478084432
1105	25	0.5761279439559033
1106	1	0.0684694293697028
1106	2	0.08005561962101027
1106	3	0.13955130206694122
1106	4	-1.151869485394812
1106	5	-0.3365663984922307
1106	6	-0.16286556870515767
1106	7	-0.008112131959819635
1106	8	0.1368170594667788
1106	9	0.3785408189991841
1106	10	0.4226752624637129
1106	11	0.2205693934286188
1106	12	0.11300186083782648
1106	13	0.09294705175444307
1106	14	0.5007679366838005
1106	15	0.36992981833807903
1106	16	0.11834050231158244
1106	17	-0.015266447065918953
1106	18	0.16606845302838735
1106	19	0.18226978089451507
1106	20	0.23128107544210508
1106	21	0.44044921692365335
1106	22	0.41629751215540267
1106	23	0.7670591298076417
1106	24	0.3311030466358318
1106	25	0.42430362939686006
1107	1	0.21692368725214806
1107	2	0.3064142354951329
1107	3	0.21836748723067245
1107	4	0.33236099594942914
1107	5	0.36752154951973637
1107	6	0.14274795410837982
1107	7	0.14088135366650079
1107	8	0.3054660741115569
1107	9	0.4425737003201533
1107	10	0.392144921563343
1107	11	0.16189804265485463
1107	12	0.21802696088093398
1107	13	0.17687184948618295
1107	14	0.6285136671638227
1107	15	0.43996164963616735
1107	16	0.463122258891417
1107	17	0.598425639676023
1107	18	0.5265850140315393
1107	19	0.435079319439879
1107	20	0.6245758065489682
1107	21	0.4624372117502418
1107	22	0.5573206100138322
1107	23	0.8166885801722401
1107	24	0.7483747327821391
1107	25	0.6889474150863961
1108	1	0.4028978581917322
1108	2	0.37715677610989573
1108	3	0.4150688230917048
1108	4	0.5759082954317876
1108	5	0.539337147036477
1108	6	0.3359340942291098
1108	7	0.390619083361905
1108	8	0.3905737550262316
1108	9	0.1276763060336492
1108	10	0.5075689608107368
1108	11	0.26395178934475894
1108	12	0.2912607986088034
1108	13	0.2617778762566303
1108	14	-1.0270077107285402
1108	15	-0.0914671138808103
1108	16	0.2568935480451202
1108	17	0.440263184343209
1108	18	0.48551503164929954
1108	19	0.449248259889508
1108	20	0.5326599676197645
1108	21	0.6520464568481046
1108	22	0.6928132789785764
1108	23	0.8169725516038113
1108	24	0.8506158982958993
1108	25	0.6132330341015688
1109	1	0.30707679037974955
1109	2	0.264296518891328
1109	3	0.42141936478408226
1109	4	0.4452613956409692
1109	5	0.4791427102938073
1109	6	0.2613559774725758
1109	7	0.2311154105062272
1109	8	0.2822969436968279
1109	9	0.39057947605019905
1109	10	0.512908627738824
1109	11	0.3323870634430861
1109	12	0.3544883735123747
1109	13	0.3582737170248578
1109	14	0.7559750934165379
1109	15	0.5060500235376821
1109	16	0.46502869559381566
1109	17	0.4565018637100806
1109	18	0.4931619660298594
1109	19	0.5054441931443492
1109	20	0.6904943401816013
1109	21	0.5481942419532544
1109	22	0.6070615194382398
1109	23	0.9822735551013579
1109	24	0.8981059757451249
1109	25	0.6494854600805624
1110	1	0.42135810604970914
1110	2	0.2832281611035228
1110	3	0.42757720691116674
1110	4	0.6266534915483888
1110	5	0.4580286948744572
1110	6	0.3260167287341531
1110	7	0.28176745030929595
1110	8	0.282726688456151
1110	9	0.4656814414616832
1110	10	0.4542858563249865
1110	11	0.2917126236892784
1110	12	0.15553920281639222
1110	13	0.16752400595893135
1110	14	0.5945197234214774
1110	15	0.4552493105220705
1110	16	0.4161343426466857
1110	17	0.38085446994490013
1110	18	0.5025072650642528
1110	19	0.47517973736857755
1110	20	0.5003034732474276
1110	21	0.5877648490831859
1110	22	0.7221493213069934
1110	23	0.8782720243830724
1110	24	0.7132411726986447
1110	25	0.7456359015366374
1111	1	0.30461592458651054
1111	2	0.24880012653579087
1111	3	0.3161548008402697
1111	4	0.6628390914922662
1111	5	0.41549280676284894
1111	6	0.33726268583639296
1111	7	0.2569408037483285
1111	8	0.3464550184258499
1111	9	0.35717900805034475
1111	10	0.2639394639134238
1111	11	0.23886250643711548
1111	12	0.2602728459093129
1111	13	0.18487052249405167
1111	14	-1.6561598492729743
1111	15	-0.49533046441365514
1111	16	0.07851046665655304
1111	17	0.17369013685653556
1111	18	0.29429152532036085
1111	19	0.3252448001855299
1111	20	0.36804720784253675
1111	21	0.4851123090178149
1111	22	0.34335307186429986
1111	23	-1.6800335272962457
1111	24	-0.20204778044085714
1111	25	0.3094126563239712
1112	1	-0.20289456814418472
1112	2	-0.3260176373775856
1112	3	-0.022528734071382672
1112	4	0.12522605690432442
1112	5	-0.809536789414075
1112	6	-0.9325096318318913
1112	7	-0.9645967827828628
1112	8	-0.7970351001826046
1112	9	-0.644565827505204
1112	10	-0.9855909309019566
1112	11	-1.005785056903612
1112	12	-0.899381570637118
1112	13	-1.0019891935760867
1112	14	-0.7544436391137737
1112	15	-0.8753964197519721
1112	16	-0.903046145730606
1112	17	-0.8955245413459423
1112	18	-1.1990812262760144
1112	19	-0.9728221988321408
1112	20	-1.1002971890212336
1112	21	-1.9300243617316681
1112	22	-1.91043591002866
1112	23	-1.4362854914127325
1112	24	-1.7178505438092984
1112	25	-2.0623653012547223
1113	1	0.030236657802110045
1113	2	-0.010601395606052222
1113	3	-0.10986538118069333
1113	4	0.20542028937492063
1113	5	0.04779230529304357
1113	6	-0.0627795344998674
1113	7	0.016182722573077513
1113	8	0.008675740960100597
1113	9	0.2052991828767432
1113	10	-0.08382188684117153
1113	11	-0.14780744403348223
1113	12	-0.04321394489658559
1113	13	-0.12140521538825766
1113	14	-0.045495288495764896
1113	15	-0.16996379936076522
1113	16	-0.04388626520473338
1113	17	0.0018914790740822696
1113	18	-0.07136120948671021
1113	19	-0.1608297637636582
1113	20	-0.6408489946718205
1113	21	-0.5003118889916607
1113	22	0.0025702731960990377
1113	23	-0.14461024331458106
1113	24	0.09140186582464338
1113	25	-1.0514219609768887
1114	1	-0.8915007675121167
1114	2	-0.7110573084360752
1114	3	-0.5223403660931041
1114	4	-1.56352085509603
1114	5	-0.729298846522596
1114	6	-0.5592602772516315
1114	7	-0.39496261337131244
1114	8	-0.3362211479881765
1114	9	-0.46495182746068764
1114	10	-0.47120853083073655
1114	11	-0.5381734994389973
1114	12	-0.5403373204368194
1114	13	-0.6000388296928847
1114	14	-0.6341023734233957
1114	15	-1.4039195491475027
1114	16	-0.8540526666956225
1114	17	-0.8239557818872001
1114	18	-0.7501961888861786
1114	19	-0.8284232459700362
1114	20	-1.9637425063896998
1114	21	-6.45938094501307
1114	22	-7.420638855590841
1114	23	-7.6819652686316395
1114	24	-5.862649295255931
1114	25	-7.215535564096346
1115	1	-1.1321190778265944
1115	2	-1.2525094750339214
1115	3	-0.9078707347751447
1115	4	-0.6554874483233064
1115	5	-0.7658505071945783
1115	6	-0.9293966155328448
1115	7	-1.1778010458070725
1115	8	-0.8782069047308383
1115	9	-2.7985986478339164
1115	10	-1.7892024492161198
1115	11	-1.2998476232373477
1115	12	-1.2274458436621387
1115	13	-1.610339643683858
1115	14	-1.263430237740728
1115	15	-2.011759600238136
1115	16	-1.5153650947050963
1115	17	-3.2181049218041835
1115	18	-2.864675498175586
1115	19	-2.2762806562852376
1115	20	-1.9070304992757698
1115	21	-1.3682368661657875
1115	22	-0.8946180729678161
1115	23	-0.3375039094324279
1115	24	-1.4646303250735926
1115	25	-1.1504645628756762
1116	1	-1.6306939613777303
1116	2	-0.9733825168151071
1116	3	-0.727258636729939
1116	4	-0.44968501298884433
1116	5	-0.3817867139277311
1116	6	-0.540952168748305
1116	7	-0.9857411558148286
1116	8	-1.8224637907460917
1116	9	-1.0252525375944899
1116	10	-2.592242931649629
1116	11	-1.3590975416266105
1116	12	-0.9222655804719153
1116	13	-1.0018579192227506
1116	14	-1.397746058990407
1116	15	-1.3727384376098826
1116	16	-0.9400524290704126
1116	17	-0.8918971707151613
1116	18	-1.358666842172724
1116	19	-0.8355962142744886
1116	20	-1.0595525341735694
1116	21	-0.7430935647396169
1116	22	-0.30780812242566924
1116	23	-0.17797739208707242
1116	24	-0.2593812631582466
1116	25	-0.3553490968520663
1201	1	0.3124233997375825
1201	2	0.4177164736464398
1201	3	0.24857882818441013
1201	4	0.3851749370084363
1201	5	0.2831923103532574
1201	6	0.23594780360692624
1201	7	0.2219845475628759
1201	8	0.3989875393304061
1201	9	0.6823604679566557
1201	10	0.5969094233011063
1201	11	0.4457668274976143
1201	12	0.48762156027223824
1201	13	0.4689945081693736
1201	14	1.1473143054765842
1201	15	0.964190329695839
1201	16	0.879499382670744
1201	17	0.8038489655755541
1201	18	0.6901128532505538
1201	19	0.6498821108158594
1201	20	0.7177606971768071
1201	21	0.8719729340405848
1201	22	0.983175446089966
1201	23	0.7477955011092874
1201	24	0.8057001678317377
1201	25	0.7622913920365519
1202	1	-0.31041825028194125
1202	2	-0.05427747535084748
1202	3	-0.12987932675155278
1202	4	-0.23195553822937143
1202	5	-0.10742342245051617
1202	6	-0.1889039950221587
1202	7	-0.2745812723371728
1202	8	0.0534007287852134
1202	9	0.09944589509233737
1202	10	0.32053095629392203
1202	11	0.06379334686288729
1202	12	-0.0043632217313458455
1202	13	0.12683105831096242
1202	14	0.24930202839338023
1202	15	0.31634895016164677
1202	16	0.2615028625874558
1202	17	0.11908092887610877
1202	18	0.19072710084397215
1202	19	0.20402835584015833
1202	20	0.3729278030132903
1202	21	0.308951819463978
1202	22	0.21122359444576233
1202	23	0.5849682507451135
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2211	6	0.21570440738582164
2211	7	0.15876338492481779
2211	8	0.37500863669733775
2211	9	0.472835906276039
2211	10	0.42194520385987033
2211	11	0.19229678650621906
2211	12	0.24887831898968926
2211	13	0.17869028600315318
2211	14	0.5075867521755058
2211	15	0.2836909251341737
2211	16	0.11564484084075133
2211	17	0.3202562759426115
2211	18	0.32875844907674434
2211	19	0.3245049027404079
2211	20	0.43642924641732606
2211	21	0.4918793037235858
2211	22	0.48735336137655594
2211	23	0.5903845546095141
2211	24	0.5522673040566914
2211	25	0.6979052763000553
2212	1	-0.38403625602679625
2212	2	-0.36982773606612085
2212	3	-0.24571317374117996
2212	4	-0.6452659798570721
2212	5	-0.2938987981490371
2212	6	-0.5473281323407662
2212	7	-0.530167443345043
2212	8	-0.3826196975584466
2212	9	-0.22245495037227414
2212	10	-0.5859344916967189
2212	11	-0.3979326709883537
2212	12	-0.5789549390977413
2212	13	-0.633786309022419
2212	14	-0.5443185439858524
2212	15	-0.6876548865190912
2212	16	-0.5262028034626383
2212	17	-0.5669923690062362
2212	18	-0.5088662753757731
2212	19	-0.6007611702586146
2212	20	-0.5967605697376968
2212	21	-0.5380656892742977
2212	22	-0.5873138192620999
2212	23	-0.06409927602172161
2212	24	-0.3088572646543142
2212	25	-1.0733548150249632
2213	1	-0.10995536983229925
2213	2	-1.1318237100300514
2213	3	-0.13110455314453517
2213	4	0.14268077090728326
2213	5	0.0995893655284803
2213	6	0.005988657502967953
2213	7	0.07112449432818124
2213	8	0.21217209212733934
2213	9	0.1448457537952379
2213	10	-0.454797204547134
2213	11	-0.09432914028496242
2213	12	-0.021347485390599275
2213	13	-0.46144047232959906
2213	14	0.025593859111531003
2213	15	-0.20151662007656768
2213	16	-0.07478982712652993
2213	17	0.09722424281516595
2213	18	0.02032637414378513
2213	19	-0.006080674906492334
2213	20	0.2749684582059331
2213	21	-0.48536709328390515
2213	22	-0.8105709815870767
2213	23	-0.1110349882349837
2213	24	0.12471617769887189
2213	25	-0.6082186155466403
2214	1	-0.18461468303850737
2214	2	-0.42353954443638386
2214	3	-0.35431865730558343
2214	4	-0.5141408143153043
2214	5	-0.19221846365923767
2214	6	-0.13514598634330355
2214	7	-0.425610756825769
2214	8	-0.11266754834196746
2214	9	-0.021196244801501507
2214	10	-1.8563770550334766
2214	11	-0.7737740624682061
2214	12	-0.42924966766204875
2214	13	-0.3675270285413035
2214	14	-0.7153775336271408
2214	15	-0.518988916146057
2214	16	-0.7777699442282895
2214	17	-0.5282525940686327
2214	18	-0.5250366187880857
2214	19	-1.3183446888875139
2214	20	-2.3968316593812204
2214	21	-1.8690913197132915
2214	22	-2.1445529245911956
2214	23	-2.5724639213644522
2214	24	-1.5771122281469019
2214	25	-1.9537570818280088
2215	1	-0.29456359031475543
2215	2	-0.4315331718444462
2215	3	-0.8241086749442808
2215	4	-0.3231776090317382
2215	5	-0.17032223951529346
2215	6	-0.11373765623611831
2215	7	-0.15971409524824765
2215	8	-0.2113077238326042
2215	9	-0.23037967619395516
2215	10	-0.3029037590273731
2215	11	-0.5182059615008185
2215	12	-0.45551721417799484
2215	13	-0.2226397032996914
2215	14	-0.14770326121932698
2215	15	-0.6162721928478874
2215	16	-0.515827114561219
2215	17	-0.23999372982019906
2215	18	-0.29892619551959854
2215	19	-2.1247460339738424
2215	20	-2.285335297958518
2215	21	-3.120689590448073
2215	22	-2.45460413346715
2215	23	-3.5115838405137545
2215	24	-2.429890791534831
2215	25	-1.8418522153027794
2216	1	-1.4551689260896374
2216	2	-1.1976050637785356
2216	3	-0.8213414735393769
2216	4	-0.8921999984826385
2216	5	-1.8462758286295298
2216	6	-1.5242460581884103
2216	7	-1.0689638609013556
2216	8	-5.26517643011017
2216	9	-4.305944838737322
2216	10	-2.2616945341084715
2216	11	-1.1619489991682457
2216	12	-1.3321015321036214
2216	13	-2.0884078701930093
2216	14	-3.364264970338951
2216	15	-2.1459871374383823
2216	16	-3.1278585029666033
2216	17	-2.7462907049285405
2216	18	-2.703521788848171
2216	19	-1.6946943739649143
2216	20	-1.002516461835441
2216	21	-0.7502954925671403
2216	22	-0.6788375385344168
2216	23	-1.1273585689515215
2216	24	-0.6519455390384508
2216	25	-0.6292079027736364
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	16.0
7	141.0
8	653.0
9	1362.0
10	2343.0
11	3860.0
12	6266.0
13	8972.0
14	12979.0
15	18393.0
16	23050.0
17	25061.0
18	25235.0
19	25645.0
20	26231.0
21	27410.0
22	28254.0
23	29220.0
24	30866.0
25	32518.0
26	33398.0
27	36967.0
28	41268.0
29	48187.0
30	56777.0
31	69663.0
32	93329.0
33	131110.0
34	190572.0
35	301184.0
36	529092.0
37	1696918.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.69047218633449	19.21345211626388	11.03600778662269	24.060067910778937
2	12.630398539609466	23.159854782022595	36.46955312375837	27.740193554609572
3	21.663828312971802	29.26616371189173	26.711898476175268	22.358109498961205
4	13.521569346493711	19.803392983993483	32.704308244884736	33.97072942462807
5	11.608568563298782	40.94224723992529	34.25127717961395	13.197907017161986
6	32.391608635430366	32.96232332644813	18.29461553731873	16.351452500802775
7	24.189539646373202	34.294427712537086	22.393430620814993	19.122602020274723
8	26.898802508344655	36.066142070553944	21.24813875968295	15.786916661418458
9	26.8705125678325	18.44909058550543	20.20196027878346	34.47843656787861
10	16.500160536388343	32.579299633999526	31.43918201227724	19.481357817334892
11	28.618565048519123	28.56534372456489	19.208736956488124	23.607354270427866
12	23.415737931503724	32.06666031249587	27.855110285631223	16.662491470369183
13	28.424886015427735	23.088577757622563	22.523272758045692	25.963263468904007
14	21.283954560252987	27.538414219629193	25.394179065620882	25.78345215449694
15	31.00968357649345	25.363503125099278	21.245923707148496	22.380889591258775
16	18.854214578991936	29.76582610876384	31.011452849773562	20.368506462470663
17	25.54260272747873	30.329860398477525	26.882556922749927	17.244979951293818
18	25.50769955702103	26.582140041632602	28.280113629334902	19.630046772011468
19	22.905545140790508	31.784247927136878	28.838690596010704	16.471516336061914
20	23.426330327459816	28.29975329814376	27.1461444927852	21.127771881611224
21	19.0373512076021	30.55364292525791	30.101627497530153	20.30737836960984
22	21.605936273745684	29.694874584061854	22.563670261287662	26.1355188809048
23	17.806223355718444	31.063430237318578	27.13909035990162	23.991256047061352
24	20.63712873166062	35.64173246609754	24.277129350177614	19.444009452064233
25	22.110180035592855	27.34909319945385	21.970401286189944	28.570325478763355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12966.0
1	12966.0
2	15539.5
3	18113.0
4	18113.0
5	18113.0
6	21484.0
7	24855.0
8	24855.0
9	24855.0
10	25222.0
11	25589.0
12	25589.0
13	25589.0
14	29368.0
15	33147.0
16	33147.0
17	33147.0
18	43345.0
19	53543.0
20	53543.0
21	53543.0
22	70816.0
23	88089.0
24	88089.0
25	88089.0
26	110305.0
27	132521.0
28	132521.0
29	132521.0
30	156155.5
31	179790.0
32	179790.0
33	179790.0
34	199813.5
35	219837.0
36	219837.0
37	219837.0
38	239683.0
39	259529.0
40	259529.0
41	259529.0
42	308201.5
43	356874.0
44	356874.0
45	356874.0
46	621413.5
47	885953.0
48	885953.0
49	885953.0
50	758019.0
51	630085.0
52	630085.0
53	630085.0
54	455254.0
55	280423.0
56	280423.0
57	280423.0
58	216127.5
59	151832.0
60	151832.0
61	151832.0
62	125636.0
63	99440.0
64	99440.0
65	99440.0
66	78017.5
67	56595.0
68	56595.0
69	56595.0
70	42109.0
71	27623.0
72	27623.0
73	27623.0
74	20057.5
75	12492.0
76	12492.0
77	12492.0
78	8732.0
79	4972.0
80	4972.0
81	4972.0
82	3375.0
83	1778.0
84	1778.0
85	1778.0
86	1205.0
87	632.0
88	632.0
89	632.0
90	418.0
91	204.0
92	204.0
93	204.0
94	126.0
95	48.0
96	48.0
97	48.0
98	30.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030953546345836865
2	0.017824294626031398
3	0.025049600176331194
4	0.028591967878034592
5	0.029828985170692924
6	0.014759865423764172
7	0.0055946918463410855
8	0.004919955141254723
9	0.005735261993234078
10	0.0032893414372960153
11	0.0027551748791026453
12	0.006297542580806045
13	0.002867630996617039
14	0.0016024996745801097
15	0.0026989468203454485
16	0.0027832889084812434
17	0.0029519730847528338
18	0.0032331133785388185
19	7.590787932221574E-4
20	5.060525288147715E-4
21	0.08664743854484033
22	0.08234599204991477
23	0.08276770249059376
24	0.0013494734101727242
25	0.2383507410717574
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3556943.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.622309520720904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.43280441999516	48.492158294357985
2	6.894429345169881	7.393904486308809
3	1.2435830108028674	2.000513793599441
4	0.4651717473602703	0.99774333668988
5	0.2309577631731965	0.6192244331543248
6	0.13908551356024054	0.4474851874785386
7	0.09399637210323256	0.3528211791121074
8	0.06652737029863484	0.285388099340241
9	0.050592231334967194	0.24415850591887733
>10	0.3229579895766504	3.2256471720251367
>50	0.027747251392821894	1.0289465710113106
>100	0.023042727675390704	2.487626402297474
>500	0.003460682697014447	1.3249683629464826
>1k	0.003460682696993274	3.9688526584631845
>5k	5.856539948757848E-4	2.307517623422473
>10k+	0.0015972381678430495	24.82304389387378
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	138797	3.902142935661325	No Hit
CCCATGTACTCTGCGTTGATACCAC	132216	3.717124508320769	No Hit
GAGTACATGGGAAGCAGTGGTATCA	65562	1.8432119941196696	No Hit
CATGTACTCTGCGTTGATACCACTG	52250	1.4689580350317675	No Hit
GCGTTGATACCACTGCTTCCCATGT	42247	1.1877333991576473	No Hit
GCTTCCCATGTACTCTGCGTTGATA	38900	1.0936357428274786	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	36051	1.0135388731278516	No Hit
GTATCAACGCAGAGTACATGGGAAG	35897	1.0092093126035475	No Hit
ACTCTGCGTTGATACCACTGCTTCC	28259	0.7944743562098128	No Hit
TATCAACGCAGAGTACATGGGAAGC	26699	0.7506164703791992	No Hit
GGTATCAACGCAGAGTACATGGGAA	23435	0.658852278487454	No Hit
CAGTGGTATCAACGCAGAGTACATG	20004	0.5623930436894827	No Hit
GTGGTATCAACGCAGAGTACATGGG	18840	0.5296683134927942	No Hit
GCAGTGGTATCAACGCAGAGTACAT	16044	0.4510614873502331	No Hit
GTACATGGTAAGCAGTGGTATCAAC	15190	0.42705210626091006	No Hit
ATACCACTGCTTCCCATGTACTCTG	14509	0.4079064522540845	No Hit
CATGGGAAGCAGTGGTATCAACGCA	14094	0.3962391300619661	No Hit
GTACTCTGCGTTGATACCACTGCTT	14017	0.39407434979981404	No Hit
ACATGGGAAGCAGTGGTATCAACGC	13308	0.3741415029703878	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	13287	0.3735511083534372	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	13065	0.3673097938313884	No Hit
TACCACTGCTTCCCATGTACTCTGC	11237	0.3159173481273105	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	11186	0.3144835326290019	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	11039	0.31035077031034797	No Hit
GATACCACTGCTTCCCATGTACTCT	10979	0.308663928547632	No Hit
ACCATGTACTCTGCGTTGATACCAC	10834	0.3045873942877353	No Hit
CTTCCCATGTACTCTGCGTTGATAC	10829	0.3044468241408423	No Hit
GTTGATACCACTGCTTCCCATGTAC	10455	0.2939321771532465	No Hit
CCATGTACTCTGCGTTGATACCACT	10145	0.285216828045881	No Hit
CCACTGCTTCCCATGTACTCTGCGT	10108	0.2841766089588728	No Hit
GTACATGGGTAAGCAGTGGTATCAA	9787	0.27515200552834274	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	9602	0.269950910093302	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	9452	0.2657338056865123	No Hit
TTCCCATGTACTCTGCGTTGATACC	7972	0.22412504220618662	No Hit
TGATACCACTGCTTCCCATGTACTC	7194	0.20225232734963705	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	7140	0.20073416976319272	No Hit
CCCCATGTACTCTGCGTTGATACCA	6652	0.1870145234264367	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	6134	0.1724514562083227	No Hit
GCTTACCATGTACTCTGCGTTGATA	6001	0.16871229030096913	No Hit
ATCAACGCAGAGTACATGGGAAGCA	5750	0.16165566892694092	No Hit
GAGTACATGGTAAGCAGTGGTATCA	5142	0.14456233906475308	No Hit
AAAAAGTACTCTGCGTTGATACCAC	4576	0.12864979843646637	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	4355	0.1224365979437961	No Hit
ACTCTGCGTTGATACCACTGCTTAC	3825	0.10753616237313895	No Hit
GCGTTGATACCACTGCTTACCATGT	3734	0.1049777856996865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.811402937859842E-5	0.0
4	0.0	0.0	0.0	2.811402937859842E-5	0.0
5	0.0	0.0	0.0	2.811402937859842E-5	0.0
6	0.0	0.0	0.0	2.811402937859842E-5	0.0
7	0.0	0.0	0.0	2.811402937859842E-5	0.0
8	0.0	0.0	0.0	2.811402937859842E-5	0.0
9	0.0	0.0	0.0	8.434208813579527E-5	0.0
10	2.811402937859842E-5	0.0	0.0	1.405701468929921E-4	0.0
11	2.811402937859842E-5	0.0	0.0	1.6868417627159053E-4	0.0
12	2.811402937859842E-5	0.0	0.0	3.092543231645826E-4	0.0
13	2.811402937859842E-5	0.0	0.0	3.3736835254318106E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAA	900	0.0	13.620367	1
CGCAAAA	365	0.0	13.014854	2
TAACCCG	55	0.0030732378	12.089093	5
ACATCGG	65	8.038007E-4	11.690551	5
TTAGGAC	145	1.2187229E-9	11.13798	3
CACGGAC	60	0.0058779884	11.083382	3
TCGTCAC	60	0.0058977073	11.078396	14
ACGCAAA	455	0.0	11.068941	1
GTATCGA	115	8.784773E-7	10.743107	18
CCGGGGT	165	9.313226E-10	10.362953	4
ACCGGAG	95	1.6390409E-4	10.003703	18
GCAGGAC	190	1.0004442E-10	10.000045	3
CTAGACA	325	0.0	9.937807	4
GAACAAA	1730	0.0	9.887078	1
TACCGGG	125	2.5695572E-6	9.880878	2
GTCTTAG	195	1.6370905E-10	9.746238	1
CGGTGGT	205	4.1836756E-11	9.727372	13
CCTAGAC	225	1.8189894E-12	9.711154	3
GTGTAAC	200	2.6375346E-10	9.502582	1
CAGGACC	280	0.0	9.499374	4
>>END_MODULE