FastQCFastQC Report
Wed 25 May 2016
SRR1294714_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294714_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3556943
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1458854.101415175896831No Hit
GTACATGGGAAGCAGTGGTATCAAC1345463.782630196772903No Hit
GAGTACATGGGAAGCAGTGGTATCA720052.024350685405979No Hit
CATGTACTCTGCGTTGATACCACTG606831.706043644781488No Hit
GCGTTGATACCACTGCTTCCCATGT465121.3076397344573698No Hit
GCTTCCCATGTACTCTGCGTTGATA443141.2458450978832103No Hit
ACGCAGAGTACATGGGAAGCAGTGG440591.2386760203916678No Hit
GTATCAACGCAGAGTACATGGGAAG375161.0547259261674984No Hit
ACTCTGCGTTGATACCACTGCTTCC331720.9325985825468668No Hit
TATCAACGCAGAGTACATGGGAAGC314710.8847766185738709No Hit
GGTATCAACGCAGAGTACATGGGAA255140.71730134556556No Hit
CAGTGGTATCAACGCAGAGTACATG237060.6664711804490542No Hit
GTGGTATCAACGCAGAGTACATGGG197160.5542962032284464No Hit
GCAGTGGTATCAACGCAGAGTACAT183170.5149646761277872No Hit
CATGGGAAGCAGTGGTATCAACGCA180780.5082454231063022No Hit
ACATGGGAAGCAGTGGTATCAACGC166820.4689982380937789No Hit
ATACCACTGCTTCCCATGTACTCTG166720.4687170977999928No Hit
GGGAAGCAGTGGTATCAACGCAGAG153680.4320564034903005No Hit
CTGCTTCCCATGTACTCTGCGTTGA146590.41212355666087425No Hit
GTACATGGTAAGCAGTGGTATCAAC145260.4083843907535207No Hit
GTACTCTGCGTTGATACCACTGCTT137700.3871301845433002No Hit
AAGCAGTGGTATCAACGCAGAGTAC135460.38083264196249417No Hit
CTTCCCATGTACTCTGCGTTGATAC131450.36955891618167624No Hit
GATACCACTGCTTCCCATGTACTCT129150.3630926894245986No Hit
TACCACTGCTTCCCATGTACTCTGC127840.3594097515760022No Hit
CCATGTACTCTGCGTTGATACCACT125120.35176273558502347No Hit
GCAGAGTACATGGGAAGCAGTGGTA122230.3436377810946085No Hit
ACCATGTACTCTGCGTTGATACCAC121480.34152922889121357No Hit
CCACTGCTTCCCATGTACTCTGCGT120640.33916765042341135No Hit
ATGGGAAGCAGTGGTATCAACGCAG112630.316648312891154No Hit
GTTGATACCACTGCTTCCCATGTAC111740.31414616427645875No Hit
GTACATGGGTAAGCAGTGGTATCAA107430.30202901761428286No Hit
GTATCAACGCAGAGTACTTTTTTTT99560.27990327649332586No Hit
GGTAAGCAGTGGTATCAACGCAGAG85990.24175253862656781No Hit
TTCCCATGTACTCTGCGTTGATACC83370.23438666292937504No Hit
TGATACCACTGCTTCCCATGTACTC79180.2226068846197423No Hit
CCCCATGTACTCTGCGTTGATACCA77170.21695596471464398No Hit
GGTATCAACGCAGAGTACTTTTTTT75310.2117267552502247No Hit
TATCAACGCAGAGTACTTTTTTTTT74970.21077087825135235No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA73540.2067505720502128No Hit
GCTTACCATGTACTCTGCGTTGATA68840.19353697824227153No Hit
ATCAACGCAGAGTACATGGGAAGCA67450.18962912815864633No Hit
GAGTACATGGTAAGCAGTGGTATCA60500.17008987774052045No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57090.16050299372241839No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55580.15625777528625No Hit
GGAAGCAGTGGTATCAACGCAGAGT50440.14180716418565043No Hit
AAAAAGTACTCTGCGTTGATACCAC49320.1386583928952474No Hit
ACTCTGCGTTGATACCACTGCTTAC43070.1210871245336234No Hit
ATGGTAAGCAGTGGTATCAACGCAG43000.12089032632797321No Hit
GAGTACTTTTTTTTTTTTTTTTTTT42740.12015936156412965No Hit
CTGCGTTGATACCACTGCTTCCCAT39710.11164081066241432No Hit
TGGGAAGCAGTGGTATCAACGCAGA39690.11158458260365713No Hit
GCGTTGATACCACTGCTTACCATGT38240.10750804834376035No Hit
ACGCAGAGTACATGGTAAGCAGTGG37340.1049777856996865No Hit
GCTTACCCATGTACTCTGCGTTGAT36250.10191335649741926No Hit
CTCTGCGTTGATACCACTGCTTCCC36120.10154787411549748No Hit
TCCATGTACTCTGCGTTGATACCAC35880.10087313741041114No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC658.067056E-411.6855256
TCCGTTG752.0891872E-411.39178512
ATTAACG700.001470689410.8760743
CTAGACT2150.010.6231424
TCTAGAC1659.0221874E-1010.3817073
TAGGGGT1409.457108E-810.196324
TAGGACC1409.457108E-810.196324
TTAGGAC1606.008122E-910.1113513
ACGTACT1701.5152182E-910.0763634
CACGTAC1803.8198777E-1010.0452633
AAAAGTA9950.010.0427482
AGCACCG951.6165039E-410.0174375
TACGCAG1054.03035E-59.9697355
AGTACTC13050.09.8447235
AAAAAGT10100.09.7100751
ACGCAAA3450.09.6595231
GTACTAG1401.0274507E-69.5215291
AGGACAG4400.09.5165665
CCGGTAC1002.7092555E-49.5165653
CTTAGAC1401.0347794E-69.5165653
Produced by FastQC (version 0.11.5)