FastQCFastQC Report
Wed 25 May 2016
SRR1294713_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294713_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3522017
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1208623.431613192099868No Hit
CCCATGTACTCTGCGTTGATACCAC1191163.382039325761346No Hit
GAGTACATGGGAAGCAGTGGTATCA578411.642269188365644No Hit
CATGTACTCTGCGTTGATACCACTG462321.3126569235753263No Hit
GCGTTGATACCACTGCTTCCCATGT389771.1066670035948152No Hit
GCTTCCCATGTACTCTGCGTTGATA327590.9301204395095196No Hit
GTATCAACGCAGAGTACATGGGAAG327500.9298649041160222No Hit
ACGCAGAGTACATGGGAAGCAGTGG313490.8900865611949061No Hit
ACTCTGCGTTGATACCACTGCTTCC257390.7308028325814441No Hit
TATCAACGCAGAGTACATGGGAAGC246410.6996275145747451No Hit
GGTATCAACGCAGAGTACATGGGAA213140.6051645974451572No Hit
CAGTGGTATCAACGCAGAGTACATG171210.4861134968968066No Hit
GTGGTATCAACGCAGAGTACATGGG162910.4625474550520341No Hit
GCAGTGGTATCAACGCAGAGTACAT139770.39684646610166846No Hit
ATACCACTGCTTCCCATGTACTCTG132570.3764036346218658No Hit
GTACTCTGCGTTGATACCACTGCTT124830.354427590781078No Hit
GTACATGGTAAGCAGTGGTATCAAC122710.3484083126231361No Hit
CATGGGAAGCAGTGGTATCAACGCA122470.3477268849071427No Hit
CTGCTTCCCATGTACTCTGCGTTGA120940.3433827832176847No Hit
ACATGGGAAGCAGTGGTATCAACGC118550.33659689887925015No Hit
GGGAAGCAGTGGTATCAACGCAGAG113490.3222301312003889No Hit
TACCACTGCTTCCCATGTACTCTGC100010.2839566078187584No Hit
CTTCCCATGTACTCTGCGTTGATAC100000.2839282149972587No Hit
GATACCACTGCTTCCCATGTACTCT99590.2827641093157699No Hit
AAGCAGTGGTATCAACGCAGAGTAC96950.2752684044398423No Hit
GTTGATACCACTGCTTCCCATGTAC96660.27444501261635024No Hit
GCAGAGTACATGGGAAGCAGTGGTA93680.26598395180943196No Hit
CCATGTACTCTGCGTTGATACCACT93400.2651889528074396No Hit
CCACTGCTTCCCATGTACTCTGCGT92710.2632298481239585No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA89140.2530936108485564No Hit
ACCATGTACTCTGCGTTGATACCAC86150.24460415722013834No Hit
GTACATGGGTAAGCAGTGGTATCAA84450.23977737756518494No Hit
ATGGGAAGCAGTGGTATCAACGCAG80260.22788078535679984No Hit
TTCCCATGTACTCTGCGTTGATACC75990.21575705057641686No Hit
TGATACCACTGCTTCCCATGTACTC67660.19210583026714523No Hit
GGTAAGCAGTGGTATCAACGCAGAG61170.17367888911382312No Hit
CCCCATGTACTCTGCGTTGATACCA59600.16922121613836616No Hit
ATCAACGCAGAGTACATGGGAAGCA51810.14710320819007972No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA48220.13691018527167811No Hit
GCTTACCATGTACTCTGCGTTGATA46610.13233894101022228No Hit
GAGTACATGGTAAGCAGTGGTATCA40830.11592789018338072No Hit
AAAAAGTACTCTGCGTTGATACCAC39350.11172575260142129No Hit
GGAAGCAGTGGTATCAACGCAGAGT35970.10212897893451395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA402.7724283E-416.6170756
CTAGGAC2350.011.3184123
CGCAAAA2900.011.1379982
GCACCGC700.001498161810.8519676
GGACAGT2750.010.7039866
GTATCGA1002.384076E-510.45659818
TCTACGG750.002648864210.1333932
ACGTAGC856.5849454E-410.0608841
CACGTAC1054.111155E-59.9517353
GAACAAA17150.09.5296481
CAGGACC2900.09.4991124
TAGACAG4600.09.4984385
ACACCGT800.00453565049.4954716
CTGTCGC1502.6701673E-79.4953379
GTCTTGC3850.09.3785441
GTACAAA7750.09.3180381
TACACTA2451.8189894E-129.3045925
GAAGCGG1752.694469E-89.2240419
TTAGGCT1651.0684016E-79.211264
GTGTAGG2900.09.17429351
Produced by FastQC (version 0.11.5)