##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294713_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3522017
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05941850933712	33.0	33.0	33.0	27.0	33.0
2	31.030419501098375	33.0	33.0	33.0	27.0	33.0
3	31.120932124972708	33.0	33.0	33.0	27.0	33.0
4	34.41126320514637	37.0	37.0	37.0	27.0	37.0
5	34.84447434524024	37.0	37.0	37.0	33.0	37.0
6	34.91106175807783	37.0	37.0	37.0	33.0	37.0
7	34.99665930062234	37.0	37.0	37.0	33.0	37.0
8	34.9075569481919	37.0	37.0	37.0	33.0	37.0
9	34.59064734781235	37.0	37.0	37.0	27.0	37.0
10	34.73616368120881	37.0	37.0	37.0	27.0	37.0
11	34.96632668155775	37.0	37.0	37.0	33.0	37.0
12	34.920875452900994	37.0	37.0	37.0	33.0	37.0
13	34.76100257324141	37.0	37.0	37.0	27.0	37.0
14	36.0619284347577	40.0	37.0	40.0	27.0	40.0
15	36.27062248705784	40.0	37.0	40.0	27.0	40.0
16	36.259922936203886	40.0	37.0	40.0	27.0	40.0
17	36.26334370333817	40.0	37.0	40.0	27.0	40.0
18	36.24766348373674	40.0	37.0	40.0	27.0	40.0
19	36.24319473756089	40.0	37.0	40.0	27.0	40.0
20	36.17666325858166	40.0	37.0	40.0	27.0	40.0
21	36.0993666413308	40.0	37.0	40.0	27.0	40.0
22	36.024811634923964	40.0	37.0	40.0	27.0	40.0
23	35.69140978024808	40.0	37.0	40.0	27.0	40.0
24	36.03473776532027	40.0	37.0	40.0	27.0	40.0
25	34.98478684231223	37.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.4287002264970532
1101	2	0.5165518294240101
1101	3	0.37933120161362055
1101	4	0.38310410278331375
1101	5	0.3866961132533291
1101	6	0.24031532719321547
1101	7	0.3012094855442058
1101	8	0.4415615493047724
1101	9	0.6979325175294591
1101	10	0.6385863469128736
1101	11	0.3592186160206481
1101	12	0.477868859150945
1101	13	0.4319980740247189
1101	14	1.0420085307365312
1101	15	0.6793252585796807
1101	16	0.7080410083671538
1101	17	0.760132577794586
1101	18	0.7841581170183289
1101	19	0.735270443181598
1101	20	0.7523815877668483
1101	21	0.8399856567649238
1101	22	0.879573029607414
1101	23	1.209224238109762
1101	24	1.029584236608109
1101	25	0.9253375464451068
1102	1	0.41165431980678946
1102	2	0.5486561724626888
1102	3	0.39162173394215927
1102	4	0.34987336264936175
1102	5	0.33068725186947745
1102	6	0.3511036702361423
1102	7	0.3405552618383183
1102	8	0.4453894506190892
1102	9	0.6704525193877089
1102	10	0.7139837534578035
1102	11	0.5195076172201354
1102	12	0.5235164013768738
1102	13	0.5748921368794484
1102	14	1.1672810383906977
1102	15	0.8794194455957438
1102	16	0.6475768698257056
1102	17	0.8416217727021191
1102	18	0.8694129437587108
1102	19	0.7370629624494356
1102	20	0.9009252981302893
1102	21	0.8731213995534901
1102	22	0.916290821367177
1102	23	1.1797057110115432
1102	24	0.9678776193290517
1102	25	0.948963641928728
1103	1	0.3504490416040902
1103	2	0.5465180773356053
1103	3	0.392037538023164
1103	4	0.1907400182047212
1103	5	0.36857985858682696
1103	6	0.30797900714667037
1103	7	0.3527815256014648
1103	8	0.4595729898266683
1103	9	0.5488602329380896
1103	10	0.49841893281048044
1103	11	0.4098809389210061
1103	12	0.40959520728807064
1103	13	0.3971081139859791
1103	14	1.0794363467628756
1103	15	0.8357493851868156
1103	16	0.5059531492834708
1103	17	0.6579295043731292
1103	18	0.6229890811587779
1103	19	0.7451643182800325
1103	20	0.8819703925951359
1103	21	0.9453777875587406
1103	22	0.7829429358575482
1103	23	1.135735251001556
1103	24	0.8300902375273012
1103	25	0.8213097786642081
1104	1	0.3253977636610834
1104	2	0.491643662077891
1104	3	0.352962542667246
1104	4	0.36426048639354036
1104	5	0.231563101134725
1104	6	0.22716648358287017
1104	7	0.26767732813821254
1104	8	0.38131463719805936
1104	9	0.5539184216911153
1104	10	0.5288514434940979
1104	11	0.2977371207177768
1104	12	0.3810787981235677
1104	13	0.41759538400368257
1104	14	0.8551361999193006
1104	15	0.6067645065558693
1104	16	0.5683554323751352
1104	17	0.5268359377373457
1104	18	0.5963591122693046
1104	19	0.6888473048428594
1104	20	0.7442276956834561
1104	21	0.8361632306665427
1104	22	0.8800881506264844
1104	23	1.1508781142537927
1104	24	0.9791895899989598
1104	25	0.8523073007809501
1105	1	0.2111176351040278
1105	2	0.3491059563290868
1105	3	0.23874212003666173
1105	4	0.1678922814012509
1105	5	0.20834448570060005
1105	6	0.17839955658865136
1105	7	0.19150792931863947
1105	8	0.23281165408609894
1105	9	-1.5462288771982102
1105	10	-0.1434113674317956
1105	11	0.03692175619853799
1105	12	0.11046015735082904
1105	13	0.14912899625592502
1105	14	-1.5948356946080011
1105	15	-0.1900655456212803
1105	16	-0.08670114991200961
1105	17	0.1856155486003317
1105	18	0.1608959034980657
1105	19	0.25223970821027564
1105	20	0.3179667720473347
1105	21	0.4967324174572667
1105	22	0.49598973195978857
1105	23	0.8968050050749525
1105	24	0.5678975246253373
1105	25	0.22205547283304128
1106	1	0.07025352454081357
1106	2	0.09713411342896094
1106	3	0.10980167931810669
1106	4	-1.104812789531131
1106	5	-0.20349225970852558
1106	6	-0.05523772057632215
1106	7	0.0834760968902799
1106	8	0.2598999917729472
1106	9	0.4721439002854453
1106	10	0.38739561510893594
1106	11	0.3141670245777277
1106	12	0.22288342393027705
1106	13	0.2794233288118946
1106	14	0.7452025608944197
1106	15	0.43878578296620674
1106	16	0.37948531758685533
1106	17	0.13731145017153068
1106	18	0.30264005104792346
1106	19	0.19751815080441304
1106	20	0.32517945834784
1106	21	0.4264674291906019
1106	22	0.6408304896025072
1106	23	0.7622398533145116
1106	24	0.36159666981977523
1106	25	0.2697043564520172
1107	1	0.3277249822309187
1107	2	0.29931223993773415
1107	3	0.36066400329493575
1107	4	0.4774354023786316
1107	5	0.4356317504100602
1107	6	0.32526109103493894
1107	7	0.3021573397093249
1107	8	0.3757420815403023
1107	9	0.5032139846644412
1107	10	0.4295906842750554
1107	11	0.31006250870053975
1107	12	0.34201327892613875
1107	13	0.282518397647209
1107	14	0.7228452088936947
1107	15	0.5410588604377367
1107	16	0.486359821264152
1107	17	0.4447928669614285
1107	18	0.5252605792249483
1107	19	0.3782531776373972
1107	20	0.60950157184989
1107	21	0.6064381125258791
1107	22	0.49099008765880825
1107	23	0.837262953376829
1107	24	0.8162848551063888
1107	25	0.6276174270914581
1108	1	0.5029618121952879
1108	2	0.38587413816773264
1108	3	0.46563388009204587
1108	4	0.6030227046799297
1108	5	0.5602796832411556
1108	6	0.44070426972462684
1108	7	0.4069837953237396
1108	8	0.5147340757938608
1108	9	0.2917395541310839
1108	10	0.5373345971331887
1108	11	0.41826297967933357
1108	12	0.3683114070378437
1108	13	0.47466102536223787
1108	14	-0.7841422636856308
1108	15	0.12489606627391936
1108	16	0.48055393003131996
1108	17	0.5735726032392634
1108	18	0.8014777874433605
1108	19	0.6362887225248173
1108	20	0.7982833314868145
1108	21	0.6854578922046457
1108	22	0.8452092771484558
1108	23	1.1032898571069794
1108	24	1.0261875647744887
1108	25	1.0278798188118756
1109	1	0.20238335097489468
1109	2	0.055893328050061086
1109	3	0.16754234100535115
1109	4	0.3075018036006725
1109	5	0.3393238471024418
1109	6	0.17691522934408965
1109	7	0.17774701567297058
1109	8	0.26760510652799496
1109	9	0.3325753066480104
1109	10	0.2937091275163013
1109	11	0.17488408198924787
1109	12	0.1439711681975453
1109	13	0.07819627775255356
1109	14	0.45550866754390285
1109	15	0.2789953680584034
1109	16	0.17682081752572287
1109	17	0.2192151033974028
1109	18	0.2457532892302794
1109	19	0.2717418279245507
1109	20	0.3529366660837283
1109	21	0.353778532765773
1109	22	0.45948210537646617
1109	23	0.7891992736315032
1109	24	0.5830308524934154
1109	25	0.4747870860167396
1110	1	0.30770812976520645
1110	2	0.18685025593454085
1110	3	0.30653565558425555
1110	4	0.41307622075657235
1110	5	0.30336079571905117
1110	6	0.18575091166709967
1110	7	0.13709263105874925
1110	8	0.23308793238020087
1110	9	0.30847834859118706
1110	10	0.3685791578518618
1110	11	0.19410095278774264
1110	12	0.09352575806683916
1110	13	0.13561231562437115
1110	14	0.5061716699965402
1110	15	0.1935722830760369
1110	16	0.12812932290107426
1110	17	0.2624488328678467
1110	18	0.35363807203593467
1110	19	0.25873160592849587
1110	20	0.35051255113082647
1110	21	0.30275632529779983
1110	22	0.420087391300612
1110	23	0.6650356038255154
1110	24	0.5852792960038684
1110	25	0.5030449979852207
1111	1	0.08175664130708782
1111	2	0.07667076761419267
1111	3	0.20742809126292272
1111	4	0.47579773901989597
1111	5	0.2955344119235761
1111	6	0.19167455134412137
1111	7	0.21810804624927016
1111	8	0.32386065789118135
1111	9	0.2551897654659925
1111	10	0.13017468413841016
1111	11	0.20655017627765204
1111	12	0.1536742287812345
1111	13	0.12320801748053611
1111	14	-1.8597457670843056
1111	15	-0.5627914600297999
1111	16	0.031162388109954975
1111	17	0.16946511431277145
1111	18	0.27522240676201903
1111	19	0.240368548018985
1111	20	0.3218307856627831
1111	21	0.3498109847669184
1111	22	0.2001847599734461
1111	23	-1.7353472822262006
1111	24	-0.30732819050750493
1111	25	0.028887910176273124
1112	1	-0.14400750910169435
1112	2	-0.2272435849141594
1112	3	0.04750737195091759
1112	4	0.20182882007752312
1112	5	-0.7344928738226457
1112	6	-0.912871867150379
1112	7	-1.004000449267103
1112	8	-0.7702318357071007
1112	9	-0.6356983475002664
1112	10	-0.8564608595886085
1112	11	-0.8592030680776048
1112	12	-0.8367361035663166
1112	13	-0.8698877229300592
1112	14	-0.5341255360742139
1112	15	-0.8168352031983872
1112	16	-0.8671156306387928
1112	17	-0.8399609386383418
1112	18	-0.9464299272627841
1112	19	-0.8217033207467921
1112	20	-0.8911761081113667
1112	21	-1.6247657043890769
1112	22	-1.5814713762933152
1112	23	-1.3370869333872335
1112	24	-1.6245349455093816
1112	25	-1.7817332858827442
1113	1	-0.02003732003743508
1113	2	-0.13653514543120693
1113	3	-0.12277113701014386
1113	4	0.0754496476605695
1113	5	0.03318155165739256
1113	6	-0.09227094849222794
1113	7	-0.0302950576915606
1113	8	-8.69454158355154E-4
1113	9	0.06305863994803218
1113	10	-0.127683829739361
1113	11	-0.11319526465558738
1113	12	-0.13125076240378775
1113	13	-0.1768635000869736
1113	14	-0.05033652107795206
1113	15	-0.3112720680609655
1113	16	-0.16307765043185185
1113	17	-0.18331666784300182
1113	18	-0.09053578724657996
1113	19	-0.23246457569696588
1113	20	-0.7564504836297203
1113	21	-0.6909763890862664
1113	22	-0.23484667907083434
1113	23	-0.35844595989951955
1113	24	-0.09650788899699592
1113	25	-1.3462022217356946
1114	1	-0.9739471977665524
1114	2	-0.7881122212700795
1114	3	-0.5823722927856636
1114	4	-1.6388080539228724
1114	5	-0.8662427154678056
1114	6	-0.6178691056518701
1114	7	-0.48092526735109686
1114	8	-0.3711218629744266
1114	9	-0.5518122700449481
1114	10	-0.5586692425923516
1114	11	-0.5340914543295909
1114	12	-0.5353864934148405
1114	13	-0.6091327895711203
1114	14	-0.59994311966053
1114	15	-1.3998292095401936
1114	16	-0.9291064077324549
1114	17	-0.9127331917054775
1114	18	-0.837615816110592
1114	19	-0.8042256685039604
1114	20	-1.976339315570776
1114	21	-6.587956121177747
1114	22	-7.571273506326957
1114	23	-7.720731909061282
1114	24	-5.700917686003848
1114	25	-7.096901169973549
1115	1	-1.0854724213868678
1115	2	-1.1724277448607623
1115	3	-0.9492125265428655
1115	4	-0.7307426028732706
1115	5	-0.6620957736626849
1115	6	-0.9145821201125415
1115	7	-1.0066260598666332
1115	8	-0.8969298497642768
1115	9	-2.805455025923976
1115	10	-1.7086811011615808
1115	11	-1.1753567523186206
1115	12	-1.1027324236031077
1115	13	-1.499022017536852
1115	14	-1.0852895267852105
1115	15	-1.9471639699495
1115	16	-1.3755552046646216
1115	17	-3.0123672781407222
1115	18	-2.732225954189609
1115	19	-2.0799473796711894
1115	20	-1.7619789433929611
1115	21	-1.3810457658011686
1115	22	-0.9528169217322358
1115	23	-0.4188176586450254
1115	24	-1.385311187018658
1115	25	-1.0072410141383585
1116	1	-1.5891388588567033
1116	2	-0.9219769581535786
1116	3	-0.7342326093529827
1116	4	-0.45628575526748705
1116	5	-0.3883406192304264
1116	6	-0.4764512994704262
1116	7	-0.9278786525411817
1116	8	-1.8598859007833113
1116	9	-1.0329600034197455
1116	10	-2.4050386966368436
1116	11	-1.2780834592345869
1116	12	-0.8965858273695773
1116	13	-0.9036299829618599
1116	14	-1.273572227836297
1116	15	-1.2856387517488201
1116	16	-0.9248584597214844
1116	17	-0.8196966443072853
1116	18	-1.4593424932955728
1116	19	-0.9641412140071353
1116	20	-1.2874760331544763
1116	21	-0.7225572033107923
1116	22	-0.2628705491200165
1116	23	-0.20937124871752388
1116	24	-0.23245277667538744
1116	25	-0.3032700262230108
1201	1	0.2977958858789407
1201	2	0.39428323623597805
1201	3	0.3749550518799438
1201	4	0.42971987364855835
1201	5	0.45324809661707377
1201	6	0.3405990180092715
1201	7	0.3920191937628985
1201	8	0.5082109866514486
1201	9	0.6347957745362862
1201	10	0.6227121673591256
1201	11	0.4444724076005073
1201	12	0.4316015507691162
1201	13	0.46492104048237337
1201	14	1.0961785061924516
1201	15	0.8290072605732703
1201	16	0.8249672288868481
1201	17	0.7646406427181134
1201	18	0.629591941643973
1201	19	0.618655913689885
1201	20	0.7904016170053509
1201	21	0.7797657565306935
1201	22	0.8674057592984852
1201	23	0.6907193400748923
1201	24	0.6717913140390124
1201	25	0.6179084264095209
1202	1	-0.0517702543110552
1202	2	0.1558258972042985
1202	3	0.13993000709304226
1202	4	0.11010105944031068
1202	5	0.05892272327126591
1202	6	-0.021622158441999773
1202	7	0.04957148771578801
1202	8	0.22284909753810211
1202	9	0.29217692505798
1202	10	0.49399284775517316
1202	11	0.20984232673475844
1202	12	0.16430283794407075
1202	13	0.26130824559726307
1202	14	0.5878721397632276
1202	15	0.5533167426262793
1202	16	0.5409406614060899
1202	17	0.2943699632099026
1202	18	0.3332129459408435
1202	19	0.3595722375882744
1202	20	0.5695924228898548
1202	21	0.6176275559486157
1202	22	0.6118107791409386
1202	23	0.8441481149144394
1202	24	0.559219337261311
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2211	6	0.34290889009522374
2211	7	0.2714444077394873
2211	8	0.35227955884355566
2211	9	0.47148462670341473
2211	10	0.3687351350455046
2211	11	0.18023153799286717
2211	12	0.23897977287845862
2211	13	0.18662017789505825
2211	14	0.6140926162396738
2211	15	0.39532217334420494
2211	16	0.3176320155147252
2211	17	0.40954422997422313
2211	18	0.3352179139635538
2211	19	0.3852938791439797
2211	20	0.4471434105485699
2211	21	0.4857511954650491
2211	22	0.47210711336120426
2211	23	0.5550210078250046
2211	24	0.578315644696076
2211	25	0.527477038634423
2212	1	-0.2113693559708345
2212	2	-0.26209957240624604
2212	3	-0.22597684196912482
2212	4	-0.4312940934117222
2212	5	-0.16565792010951697
2212	6	-0.5596425383244323
2212	7	-0.5598175624075168
2212	8	-0.28974034156507145
2212	9	-0.1199727242801032
2212	10	-0.4200982557666819
2212	11	-0.44571967236424825
2212	12	-0.6161375365886883
2212	13	-0.6494699522078378
2212	14	-0.4514512986254644
2212	15	-0.6451259525299946
2212	16	-0.5479462118247724
2212	17	-0.551959837662487
2212	18	-0.4362635816343641
2212	19	-0.5057375031646032
2212	20	-0.5247588563094681
2212	21	-0.44206872870228864
2212	22	-0.43457872344817616
2212	23	0.012385882907288703
2212	24	-0.21799865388164363
2212	25	-0.8705745888287453
2213	1	-0.11012671476036928
2213	2	-1.166740503985963
2213	3	-0.20356433531826568
2213	4	0.11614431945016435
2213	5	0.008887698737709115
2213	6	0.01262021788452472
2213	7	-0.019880023992243423
2213	8	0.0981335418114071
2213	9	0.08025527842152513
2213	10	-0.3776480291116755
2213	11	-0.0816882904425853
2213	12	-0.08051450604109078
2213	13	-0.3987814335943938
2213	14	-0.025288105465861577
2213	15	-0.19698971180127245
2213	16	-0.09261905194166076
2213	17	0.06250155386445755
2213	18	0.02985227845106664
2213	19	0.10054710758785745
2213	20	0.1881087754554187
2213	21	-0.4007912031096268
2213	22	-0.7812849226319187
2213	23	-0.05379204925435488
2213	24	0.1351772193380114
2213	25	-0.5271530681719412
2214	1	-0.1991052171409784
2214	2	-0.3862928218888051
2214	3	-0.37926261950414286
2214	4	-0.47087292747835363
2214	5	-0.21630569104953423
2214	6	-0.1678193346276018
2214	7	-0.4716715570155614
2214	8	-0.13698873491634345
2214	9	0.006918264115675754
2214	10	-1.8353809014910638
2214	11	-0.7104600849444296
2214	12	-0.3807393598195432
2214	13	-0.18800970568300102
2214	14	-0.728920448451106
2214	15	-0.5108015438157452
2214	16	-0.6208188066668043
2214	17	-0.4263346114130613
2214	18	-0.4162206602968155
2214	19	-1.3360725943088596
2214	20	-2.312192518619142
2214	21	-1.7508118461514783
2214	22	-1.890129106510372
2214	23	-2.250753776388855
2214	24	-1.4426300123856848
2214	25	-1.6863101717090032
2215	1	-0.2826449874117216
2215	2	-0.3544414039007471
2215	3	-0.8815317217371685
2215	4	-0.26401873427675326
2215	5	-0.2908508481558343
2215	6	-0.2725164565850875
2215	7	-0.2681512716651824
2215	8	-0.2788014167658588
2215	9	-0.33940939804443815
2215	10	-0.27667344989663434
2215	11	-0.8155077601918634
2215	12	-0.4738696838476386
2215	13	-0.29281491490489486
2215	14	-0.22280767545773728
2215	15	-0.6484820787600611
2215	16	-0.4683127888353269
2215	17	-0.3129817444714007
2215	18	-0.41404430862175445
2215	19	-2.354685513366107
2215	20	-2.6063775698583527
2215	21	-3.5423290407903565
2215	22	-2.559727518503969
2215	23	-3.6283836563827805
2215	24	-2.5838138892713687
2215	25	-1.934366791907884
2216	1	-1.572525705576858
2216	2	-1.122482662249304
2216	3	-0.8112308164447626
2216	4	-0.9424204134809884
2216	5	-1.7849413708049724
2216	6	-1.4247658456748482
2216	7	-1.1094645641844565
2216	8	-5.207878860798171
2216	9	-3.922858482894366
2216	10	-1.9866932723315855
2216	11	-0.9868269296434917
2216	12	-1.1993803372441079
2216	13	-1.9224841953331975
2216	14	-3.3992717207669614
2216	15	-2.027711530889434
2216	16	-3.0624614687501435
2216	17	-2.6742059849282143
2216	18	-2.4430869830602973
2216	19	-1.6118599200731296
2216	20	-0.9558545370034395
2216	21	-0.7153941640982353
2216	22	-0.6686789939694506
2216	23	-0.9440412495665242
2216	24	-0.5502785336910634
2216	25	-0.3972598433917156
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	2.0
7	40.0
8	201.0
9	577.0
10	1186.0
11	2438.0
12	4178.0
13	6702.0
14	10017.0
15	14734.0
16	19231.0
17	20980.0
18	21472.0
19	21749.0
20	22060.0
21	23374.0
22	24440.0
23	25075.0
24	26374.0
25	28233.0
26	29906.0
27	32704.0
28	36608.0
29	42711.0
30	51817.0
31	62850.0
32	85336.0
33	122683.0
34	180955.0
35	293313.0
36	524820.0
37	1785251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.44952512951013	19.894091384167954	11.675622557484322	23.98076092883759
2	12.551661194569816	23.22190928757104	37.33029258537976	26.89613693247938
3	20.781399005019203	28.976557916254176	27.496213018356425	22.745830060370196
4	12.860304502157346	19.81260488188337	34.31353254252855	33.01355807343074
5	11.35857239655424	40.62154070895126	35.0800013858463	12.93988550864819
6	31.58395223400522	34.351457592005985	18.37002063696322	15.694569537025574
7	23.937448342810384	34.242736477421886	23.010593077773333	18.809222101994397
8	25.675587811154266	36.98981858923052	21.518696584078338	15.815897015536882
9	25.94062711881731	18.571259836706975	20.83465360140738	34.653459443068336
10	15.592518602981928	32.50791592088167	32.50374217493617	19.39582330120022
11	28.630117033367814	28.352830376716327	20.092464850097404	22.92458773981846
12	22.486471353682354	31.652361703148607	28.975792248147414	16.885374695021625
13	27.831487418371964	23.4048691041054	23.47982819603279	25.283815281489847
14	20.64138117253498	27.21172358945631	26.303061931128198	25.84383330688051
15	29.460425986209327	26.519749740555287	21.954346249018652	22.065478024216734
16	18.67315630499483	30.312113669751913	30.758404075956253	20.256325949297
17	24.208627230957678	30.67765916185442	27.600216700104884	17.513496907083017
18	24.377877501998192	27.399086013796392	28.930147773879984	19.292888710325432
19	22.070004503120668	31.897511699311927	29.325508616974094	16.70697518059331
20	22.771585114021896	28.658923526099812	27.86959905440064	20.699892305477658
21	19.042520392084985	30.670463054131197	30.116804271504222	20.170212282279596
22	20.986221652662895	30.056176103702736	23.810058278800554	25.14754396483382
23	17.823921474133112	30.9951444172164	27.80201906770992	23.378915040940566
24	20.246282270601014	35.15067540229559	25.227038806351548	19.37600352075185
25	21.512590933730955	27.85300356976696	23.24116059398418	27.393244902517903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11907.0
1	11907.0
2	13904.5
3	15902.0
4	15902.0
5	15902.0
6	18757.5
7	21613.0
8	21613.0
9	21613.0
10	23086.0
11	24559.0
12	24559.0
13	24559.0
14	30536.5
15	36514.0
16	36514.0
17	36514.0
18	49921.0
19	63328.0
20	63328.0
21	63328.0
22	84258.0
23	105188.0
24	105188.0
25	105188.0
26	131650.0
27	158112.0
28	158112.0
29	158112.0
30	185136.0
31	212160.0
32	212160.0
33	212160.0
34	232778.5
35	253397.0
36	253397.0
37	253397.0
38	269903.0
39	286409.0
40	286409.0
41	286409.0
42	321790.0
43	357171.0
44	357171.0
45	357171.0
46	585651.5
47	814132.0
48	814132.0
49	814132.0
50	699015.0
51	583898.0
52	583898.0
53	583898.0
54	424642.5
55	265387.0
56	265387.0
57	265387.0
58	203683.0
59	141979.0
60	141979.0
61	141979.0
62	115720.0
63	89461.0
64	89461.0
65	89461.0
66	68206.0
67	46951.0
68	46951.0
69	46951.0
70	34157.0
71	21363.0
72	21363.0
73	21363.0
74	14930.0
75	8497.0
76	8497.0
77	8497.0
78	5650.5
79	2804.0
80	2804.0
81	2804.0
82	1877.0
83	950.0
84	950.0
85	950.0
86	592.5
87	235.0
88	235.0
89	235.0
90	156.0
91	77.0
92	77.0
93	77.0
94	44.5
95	12.0
96	12.0
97	12.0
98	11.5
99	11.0
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030011212325210243
2	0.014622303072358822
3	0.02109586637429632
4	0.02254390027078234
5	0.019960153514307286
6	0.005139100691450382
7	0.0
8	2.839282149972586E-4
9	1.419641074986293E-4
10	2.839282149972587E-5
11	8.51784644991776E-4
12	0.00130606978898739
13	0.002725710863973683
14	6.814277159934208E-4
15	0.0020442831479802626
16	0.0026973180424739577
17	0.002810889328472861
18	0.002328211362977521
19	4.25892322495888E-4
20	5.110707869950657E-4
21	0.08182811156220995
22	0.07742722422975244
23	0.07861972273274093
24	0.0011924985029884865
25	0.22095293691086668
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3522017.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.92987745906287
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.23890902518353	52.275289418684046
2	7.3286442129957745	8.49094922399831
3	1.2396611164463736	2.1544024969951048
4	0.38770081973275966	0.8983784391158797
5	0.18633881850152365	0.5397292460829911
6	0.11356379898260228	0.394724217530868
7	0.08196266511141251	0.33236610022856344
8	0.06052649955911898	0.2805034161988629
9	0.04171932367002058	0.21751157770913462
>10	0.27177526968877297	2.885465136035832
>50	0.023780902248592743	0.9635225739803253
>100	0.018018854211599662	2.1203001714735223
>500	0.0025324387722528304	1.037829185196941
>1k	0.0029793397320206343	3.6071029223310442
>5k	7.944905952055025E-4	3.8235630409187387
>10k+	0.0010924245684075658	19.97836283351987
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	120862	3.431613192099868	No Hit
CCCATGTACTCTGCGTTGATACCAC	119116	3.382039325761346	No Hit
GAGTACATGGGAAGCAGTGGTATCA	57841	1.642269188365644	No Hit
CATGTACTCTGCGTTGATACCACTG	46232	1.3126569235753263	No Hit
GCGTTGATACCACTGCTTCCCATGT	38977	1.1066670035948152	No Hit
GCTTCCCATGTACTCTGCGTTGATA	32759	0.9301204395095196	No Hit
GTATCAACGCAGAGTACATGGGAAG	32750	0.9298649041160222	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	31349	0.8900865611949061	No Hit
ACTCTGCGTTGATACCACTGCTTCC	25739	0.7308028325814441	No Hit
TATCAACGCAGAGTACATGGGAAGC	24641	0.6996275145747451	No Hit
GGTATCAACGCAGAGTACATGGGAA	21314	0.6051645974451572	No Hit
CAGTGGTATCAACGCAGAGTACATG	17121	0.4861134968968066	No Hit
GTGGTATCAACGCAGAGTACATGGG	16291	0.4625474550520341	No Hit
GCAGTGGTATCAACGCAGAGTACAT	13977	0.39684646610166846	No Hit
ATACCACTGCTTCCCATGTACTCTG	13257	0.3764036346218658	No Hit
GTACTCTGCGTTGATACCACTGCTT	12483	0.354427590781078	No Hit
GTACATGGTAAGCAGTGGTATCAAC	12271	0.3484083126231361	No Hit
CATGGGAAGCAGTGGTATCAACGCA	12247	0.3477268849071427	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	12094	0.3433827832176847	No Hit
ACATGGGAAGCAGTGGTATCAACGC	11855	0.33659689887925015	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	11349	0.3222301312003889	No Hit
TACCACTGCTTCCCATGTACTCTGC	10001	0.2839566078187584	No Hit
CTTCCCATGTACTCTGCGTTGATAC	10000	0.2839282149972587	No Hit
GATACCACTGCTTCCCATGTACTCT	9959	0.2827641093157699	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	9695	0.2752684044398423	No Hit
GTTGATACCACTGCTTCCCATGTAC	9666	0.27444501261635024	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	9368	0.26598395180943196	No Hit
CCATGTACTCTGCGTTGATACCACT	9340	0.2651889528074396	No Hit
CCACTGCTTCCCATGTACTCTGCGT	9271	0.2632298481239585	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	8914	0.2530936108485564	No Hit
ACCATGTACTCTGCGTTGATACCAC	8615	0.24460415722013834	No Hit
GTACATGGGTAAGCAGTGGTATCAA	8445	0.23977737756518494	No Hit
ATGGGAAGCAGTGGTATCAACGCAG	8026	0.22788078535679984	No Hit
TTCCCATGTACTCTGCGTTGATACC	7599	0.21575705057641686	No Hit
TGATACCACTGCTTCCCATGTACTC	6766	0.19210583026714523	No Hit
GGTAAGCAGTGGTATCAACGCAGAG	6117	0.17367888911382312	No Hit
CCCCATGTACTCTGCGTTGATACCA	5960	0.16922121613836616	No Hit
ATCAACGCAGAGTACATGGGAAGCA	5181	0.14710320819007972	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA	4822	0.13691018527167811	No Hit
GCTTACCATGTACTCTGCGTTGATA	4661	0.13233894101022228	No Hit
GAGTACATGGTAAGCAGTGGTATCA	4083	0.11592789018338072	No Hit
AAAAAGTACTCTGCGTTGATACCAC	3935	0.11172575260142129	No Hit
GGAAGCAGTGGTATCAACGCAGAGT	3597	0.10212897893451395	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.839282149972587E-5	0.0
8	0.0	0.0	0.0	2.839282149972587E-5	0.0
9	0.0	0.0	0.0	8.51784644991776E-5	0.0
10	2.839282149972587E-5	0.0	0.0	8.51784644991776E-5	0.0
11	2.839282149972587E-5	0.0	0.0	1.1357128599890347E-4	0.0
12	2.839282149972587E-5	0.0	0.0	1.4196410749862934E-4	0.0
13	2.839282149972587E-5	0.0	0.0	1.4196410749862934E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGA	40	2.7724283E-4	16.617075	6
CTAGGAC	235	0.0	11.318412	3
CGCAAAA	290	0.0	11.137998	2
GCACCGC	70	0.0014981618	10.851967	6
GGACAGT	275	0.0	10.703986	6
GTATCGA	100	2.384076E-5	10.456598	18
TCTACGG	75	0.0026488642	10.133393	2
ACGTAGC	85	6.5849454E-4	10.060884	1
CACGTAC	105	4.111155E-5	9.951735	3
GAACAAA	1715	0.0	9.529648	1
CAGGACC	290	0.0	9.499112	4
TAGACAG	460	0.0	9.498438	5
ACACCGT	80	0.0045356504	9.495471	6
CTGTCGC	150	2.6701673E-7	9.495337	9
GTCTTGC	385	0.0	9.378544	1
GTACAAA	775	0.0	9.318038	1
TACACTA	245	1.8189894E-12	9.304592	5
GAAGCGG	175	2.694469E-8	9.224041	9
TTAGGCT	165	1.0684016E-7	9.21126	4
GTGTAGG	290	0.0	9.1742935	1
>>END_MODULE