FastQCFastQC Report
Wed 25 May 2016
SRR1294713_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294713_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3522017
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1272143.611964394266126No Hit
GTACATGGGAAGCAGTGGTATCAAC1196363.396803592941204No Hit
GAGTACATGGGAAGCAGTGGTATCA637691.810581834216019No Hit
CATGTACTCTGCGTTGATACCACTG526111.4937747319220775No Hit
GCGTTGATACCACTGCTTCCCATGT409491.1626576475922745No Hit
ACGCAGAGTACATGGGAAGCAGTGG388081.1018686167613614No Hit
GCTTCCCATGTACTCTGCGTTGATA362631.0296088860445591No Hit
GTATCAACGCAGAGTACATGGGAAG343720.9759180605885774No Hit
ACTCTGCGTTGATACCACTGCTTCC294960.8374746629559141No Hit
TATCAACGCAGAGTACATGGGAAGC291640.8280482462180052No Hit
GGTATCAACGCAGAGTACATGGGAA237800.6751812952634811No Hit
CAGTGGTATCAACGCAGAGTACATG205850.584466230571857No Hit
GTGGTATCAACGCAGAGTACATGGG175110.4971866972816996No Hit
GCAGTGGTATCAACGCAGAGTACAT159090.4517013972391388No Hit
CATGGGAAGCAGTGGTATCAACGCA154580.43889623474276246No Hit
ATACCACTGCTTCCCATGTACTCTG149530.4245578598854009No Hit
ACATGGGAAGCAGTGGTATCAACGC147910.41995822280244527No Hit
GGGAAGCAGTGGTATCAACGCAGAG134230.38111684299082027No Hit
CTGCTTCCCATGTACTCTGCGTTGA125020.35496705438957277No Hit
GTACTCTGCGTTGATACCACTGCTT119230.3385276107412315No Hit
AAGCAGTGGTATCAACGCAGAGTAC118110.3353476147332622No Hit
GTACATGGTAAGCAGTGGTATCAAC116940.33202565461779426No Hit
CTTCCCATGTACTCTGCGTTGATAC114680.32560887695885626No Hit
GATACCACTGCTTCCCATGTACTCT113630.322627630701385No Hit
TACCACTGCTTCCCATGTACTCTGC112320.3189081710849209No Hit
CCATGTACTCTGCGTTGATACCACT110210.3129172857484788No Hit
GCAGAGTACATGGGAAGCAGTGGTA108820.3089706835600169No Hit
CCACTGCTTCCCATGTACTCTGCGT105820.3004528371100991No Hit
GTATCAACGCAGAGTACTTTTTTTT100760.2860860694312378No Hit
GTTGATACCACTGCTTCCCATGTAC98330.27918661380680443No Hit
ATGGGAAGCAGTGGTATCAACGCAG97480.27677322397932774No Hit
ACCATGTACTCTGCGTTGATACCAC95530.27123662378688124No Hit
GTACATGGGTAAGCAGTGGTATCAA91920.2609868152254802No Hit
GGTATCAACGCAGAGTACTTTTTTT78190.22200347130635656No Hit
TTCCCATGTACTCTGCGTTGATACC78130.2218331143773582No Hit
TATCAACGCAGAGTACTTTTTTTTT77090.2188802609413867No Hit
GGTAAGCAGTGGTATCAACGCAGAG73580.20891438059498293No Hit
TGATACCACTGCTTCCCATGTACTC70450.20002742746556873No Hit
CCCCATGTACTCTGCGTTGATACCA67380.1913108312651529No Hit
ATCAACGCAGAGTACATGGGAAGCA62620.1777958482312834No Hit
ACGCAGAGTACTTTTTTTTTTTTTT55700.1581480157534731No Hit
GTACTTTTTTTTTTTTTTTTTTTTT53990.15329284327701995No Hit
GCTTACCATGTACTCTGCGTTGATA50610.1436960696101126No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA49080.13935196792065455No Hit
GAGTACATGGTAAGCAGTGGTATCA48040.13639911448468306No Hit
GGAAGCAGTGGTATCAACGCAGAGT44990.12773930392726668No Hit
AAAAAGTACTCTGCGTTGATACCAC43710.12410502277530176No Hit
GAGTACTTTTTTTTTTTTTTTTTTT39260.11147021720792376No Hit
ACTCTGCGTTGATACCACTGCTTAC35790.10161790814751889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGT655.4812794E-513.1474476
ACGTACT1800.011.6280814
GCGTCAG752.038367E-411.4220231
TACGGCT600.00581460811.0995314
TGTTCCG600.00581460811.0995315
AAGGGCG600.00581460811.0995315
ATTAACG909.382052E-510.5709833
CGTACTC1855.820766E-1110.285285
GTACTTG5150.010.1652311
TATACCG750.00261709410.1481435
CGCGATC750.002661588610.12754117
CCACGTA1701.5224941E-910.0736682
CACGTAC1909.640644E-1110.0146153
CCCACGT1553.949026E-89.8253971
CGCAAAA1553.9770384E-89.8209242
CCTACAC2151.0913936E-119.7351373
GTCTTAG2800.09.51835251
CTACGCA800.00446374349.5138844
TATACTG6900.09.3760025
CTAGGAC2650.09.3343773
Produced by FastQC (version 0.11.5)