FastQCFastQC Report
Wed 25 May 2016
SRR1294712_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294712_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4190681
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1034982.469717928899861No Hit
CCCATGTACTCTGCGTTGATACCAC1012022.4149296975837578No Hit
GAGTACATGGGAAGCAGTGGTATCA472401.1272630868348128No Hit
CATGTACTCTGCGTTGATACCACTG379920.9065829634849323No Hit
GCGTTGATACCACTGCTTCCCATGT348850.8324422689295606No Hit
GTATCAACGCAGAGTACATGGGAAG316410.7550324159724875No Hit
ACGCAGAGTACATGGGAAGCAGTGG261430.6238365554428982No Hit
GCTTCCCATGTACTCTGCGTTGATA242400.5784262748703611No Hit
ACTCTGCGTTGATACCACTGCTTCC215130.5133533189474455No Hit
TATCAACGCAGAGTACATGGGAAGC198550.4737893435458342No Hit
GGTATCAACGCAGAGTACATGGGAA170920.4078573386998438No Hit
GTGGTATCAACGCAGAGTACATGGG154960.36977283644352793No Hit
CAGTGGTATCAACGCAGAGTACATG148000.35316455726408186No Hit
ATACCACTGCTTCCCATGTACTCTG120320.2871132400676644No Hit
GCAGTGGTATCAACGCAGAGTACAT118140.2819112215890448No Hit
GTACATGGTAAGCAGTGGTATCAAC106430.2539682691190286No Hit
GTACTCTGCGTTGATACCACTGCTT105770.2523933460933915No Hit
CATGGGAAGCAGTGGTATCAACGCA103240.24635614116178253No Hit
CTGCTTCCCATGTACTCTGCGTTGA101740.2427767706489709No Hit
GGGAAGCAGTGGTATCAACGCAGAG98870.23592824173445792No Hit
ACATGGGAAGCAGTGGTATCAACGC94650.22585827935841454No Hit
TACCACTGCTTCCCATGTACTCTGC92110.21979721195672014No Hit
CCATGTACTCTGCGTTGATACCACT91100.21738710247809365No Hit
GATACCACTGCTTCCCATGTACTCT88520.21123058519605764No Hit
GCAGAGTACATGGGAAGCAGTGGTA81430.19431209390550128No Hit
CTTCCCATGTACTCTGCGTTGATAC80120.19118611032431246No Hit
AAGCAGTGGTATCAACGCAGAGTAC78220.18665224100808436No Hit
CCACTGCTTCCCATGTACTCTGCGT75570.18032868643545047No Hit
ACCATGTACTCTGCGTTGATACCAC75280.17963667480297354No Hit
GTTGATACCACTGCTTCCCATGTAC73870.1762720665209306No Hit
ATGGGAAGCAGTGGTATCAACGCAG69080.16484194335001878No Hit
GTACATGGGTAAGCAGTGGTATCAA64210.15322092041842364No Hit
TTCCCATGTACTCTGCGTTGATACC60950.14544175517057967No Hit
TGATACCACTGCTTCCCATGTACTC59690.1424350839398179No Hit
GTATCAACGCAGAGTACTTTTTTTT54030.1289289258714753No Hit
ATCAACGCAGAGTACATGGGAAGCA53120.1267574410937029No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA50600.12074409863217934No Hit
CCCCATGTACTCTGCGTTGATACCA50170.11971801241850669No Hit
GGTAAGCAGTGGTATCAACGCAGAG47870.11422964429886216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGGC1158.838397E-710.7381663
TACGTCG803.7800192E-410.685525
ACGTCGC803.7846068E-410.6841166
GTCCTAG2900.09.8294021
GTCTAGT1554.0581654E-89.8082641
GTATTAG3750.09.6284451
TTAGTAC2106.730261E-119.4991463
TAGGTCG900.00111623359.49823955
GTACAAA8200.09.38587951
TAGACAG4350.09.1707145
GTCTTAC2800.09.1624071
AGTACCG950.00182788118.9983335
GTCTTAG3300.08.9258911
TAGTACC2451.6370905E-118.9172474
GGACCGA1401.0904823E-58.8186366
GTTAGAC1201.7667892E-48.7075513
TAACACG1201.7674753E-48.7072394
GTCCTAC2304.0381565E-108.6755151
TAACCCG1107.161893E-48.6347645
TACACCG1107.161893E-48.6347645
Produced by FastQC (version 0.11.5)