FastQCFastQC Report
Wed 25 May 2016
SRR1294712_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294712_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4190681
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1082992.5842816477799193No Hit
GTACATGGGAAGCAGTGGTATCAAC1004402.396746495378675No Hit
GAGTACATGGGAAGCAGTGGTATCA519161.2388439969541942No Hit
CATGTACTCTGCGTTGATACCACTG433301.033960828800856No Hit
GCGTTGATACCACTGCTTCCCATGT371100.8855362648696No Hit
GTATCAACGCAGAGTACATGGGAAG331640.7913749579125684No Hit
ACGCAGAGTACATGGGAAGCAGTGG322640.7698987348356986No Hit
GCTTCCCATGTACTCTGCGTTGATA266370.6356246156650912No Hit
ACTCTGCGTTGATACCACTGCTTCC246220.5875417384429881No Hit
TATCAACGCAGAGTACATGGGAAGC227760.5434916186653195No Hit
GGTATCAACGCAGAGTACATGGGAA184800.4409784471783942No Hit
CAGTGGTATCAACGCAGAGTACATG178520.42599281596475613No Hit
GTATCAACGCAGAGTACTTTTTTTT165220.39425573075115955No Hit
GTGGTATCAACGCAGAGTACATGGG163890.39108202222979993No Hit
GCAGTGGTATCAACGCAGAGTACAT134290.3204491107769835No Hit
ATACCACTGCTTCCCATGTACTCTG133550.3186832879906631No Hit
CATGGGAAGCAGTGGTATCAACGCA132740.3167504279137448No Hit
ACATGGGAAGCAGTGGTATCAACGC118160.28195894652921566No Hit
GGGAAGCAGTGGTATCAACGCAGAG115720.2761365038283754No Hit
GGTATCAACGCAGAGTACTTTTTTT112440.2683096136403606No Hit
CTGCTTCCCATGTACTCTGCGTTGA108380.25862145078568377No Hit
TATCAACGCAGAGTACTTTTTTTTT106420.2539444066489432No Hit
CCATGTACTCTGCGTTGATACCACT105690.2522024463327082No Hit
GTACTCTGCGTTGATACCACTGCTT104490.24933894992245892No Hit
TACCACTGCTTCCCATGTACTCTGC101880.24311084523016666No Hit
GTACATGGTAAGCAGTGGTATCAAC100740.24039052364042982No Hit
GATACCACTGCTTCCCATGTACTCT99700.2379088267515471No Hit
AAGCAGTGGTATCAACGCAGAGTAC93720.22363906964047134No Hit
CTTCCCATGTACTCTGCGTTGATAC93070.2220880090849196No Hit
GCAGAGTACATGGGAAGCAGTGGTA90450.21583604192254194No Hit
ACCATGTACTCTGCGTTGATACCAC86120.205503592375559No Hit
CCACTGCTTCCCATGTACTCTGCGT85470.2039525318200073No Hit
ATGGGAAGCAGTGGTATCAACGCAG79690.19016002411063976No Hit
GTTGATACCACTGCTTCCCATGTAC75810.18090138571750033No Hit
ACGCAGAGTACTTTTTTTTTTTTTT69150.16500898064061664No Hit
GTACATGGGTAAGCAGTGGTATCAA68990.16462718111925007No Hit
TTCCCATGTACTCTGCGTTGATACC63960.15262435866628837No Hit
ATCAACGCAGAGTACATGGGAAGCA62610.1494029252047579No Hit
TGATACCACTGCTTCCCATGTACTC59460.14188624712785344No Hit
GGTAAGCAGTGGTATCAACGCAGAG57070.13618311677744022No Hit
CCCCATGTACTCTGCGTTGATACCA55680.1328662334355681No Hit
GTACTTTTTTTTTTTTTTTTTTTTT49330.11771356493133216No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGAC752.047294E-411.4167583
ACACCGT1604.5474735E-1111.2758586
TAAACCG600.00581455411.0996265
ATTAACG803.7226788E-410.703213
GGACCGA1201.5349306E-610.2867486
CCTAGAC2800.010.1935343
ACCGTCC856.6320645E-410.0531778
AACACCG1252.525032E-69.8945245
TCTATAC4750.09.8144053
ACGCAAA1701.6168997E-89.5179481
TACGCTG900.00109947229.5139655
TACGACC800.00446373279.5139654
TATTCCG1701.6279955E-89.5139655
TTACACT4900.09.5139654
GTACCGT900.00111922619.495466
AGACCGT800.0045369.495466
TATACTG6700.09.0879665
TACTGCG950.001834158.9949477
GTCTTAG2651.8189894E-128.9791961
CTAGACA3400.08.954324
Produced by FastQC (version 0.11.5)