FastQCFastQC Report
Wed 25 May 2016
SRR1294711_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294711_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3515539
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC1428284.062762495310108No Hit
CCCATGTACTCTGCGTTGATACCAC1371113.9001416283534334No Hit
GAGTACATGGGAAGCAGTGGTATCA650551.8504986006413242No Hit
CATGTACTCTGCGTTGATACCACTG517391.4717231127289443No Hit
GCGTTGATACCACTGCTTCCCATGT433511.2331252760956428No Hit
GCTTCCCATGTACTCTGCGTTGATA372941.0608330614452008No Hit
GTATCAACGCAGAGTACATGGGAAG365051.0383898457676048No Hit
ACGCAGAGTACATGGGAAGCAGTGG350160.996035031896958No Hit
TATCAACGCAGAGTACATGGGAAGC285670.8125923222584076No Hit
ACTCTGCGTTGATACCACTGCTTCC283490.806391281678286No Hit
GGTATCAACGCAGAGTACATGGGAA237060.6743204953778069No Hit
CAGTGGTATCAACGCAGAGTACATG189310.5384949505609239No Hit
GTGGTATCAACGCAGAGTACATGGG177270.5042470016688764No Hit
GCAGTGGTATCAACGCAGAGTACAT156960.446474921768753No Hit
GTACATGGTAAGCAGTGGTATCAAC146680.4172333175652439No Hit
ATACCACTGCTTCCCATGTACTCTG145930.41509993204455986No Hit
GTACTCTGCGTTGATACCACTGCTT137590.3913766850545535No Hit
CTGCTTCCCATGTACTCTGCGTTGA137260.39043799542545254No Hit
CATGGGAAGCAGTGGTATCAACGCA134720.38321292979540267No Hit
GGGAAGCAGTGGTATCAACGCAGAG130410.37095307433653846No Hit
ACATGGGAAGCAGTGGTATCAACGC124470.35405666101272093No Hit
TACCACTGCTTCCCATGTACTCTGC116720.332011677298986No Hit
AAGCAGTGGTATCAACGCAGAGTAC109780.31227074994758985No Hit
CTTCCCATGTACTCTGCGTTGATAC109440.3113036151782131No Hit
GCAGAGTACATGGGAAGCAGTGGTA109040.3101658095671816No Hit
GATACCACTGCTTCCCATGTACTCT107670.30626882534939875No Hit
GTTGATACCACTGCTTCCCATGTAC107180.3048750134758852No Hit
CCACTGCTTCCCATGTACTCTGCGT105750.3008073584164477No Hit
CCATGTACTCTGCGTTGATACCACT101500.2887181737992382No Hit
ACCATGTACTCTGCGTTGATACCAC101330.2882346064145498No Hit
GTACATGGGTAAGCAGTGGTATCAA91120.2591921181929713No Hit
ATGGGAAGCAGTGGTATCAACGCAG88890.2528488519114708No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA83140.23649289625289321No Hit
TTCCCATGTACTCTGCGTTGATACC79220.22534240126478472No Hit
TGATACCACTGCTTCCCATGTACTC73030.20773485943407255No Hit
GGTAAGCAGTGGTATCAACGCAGAG68890.19595857135989675No Hit
CCCCATGTACTCTGCGTTGATACCA68160.1938820761197643No Hit
ATCAACGCAGAGTACATGGGAAGCA58060.16515248444121938No Hit
GCTTACCATGTACTCTGCGTTGATA53530.15226683589628787No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA49630.14117323118873096No Hit
GAGTACATGGTAAGCAGTGGTATCA48260.13727624697094812No Hit
GGAAGCAGTGGTATCAACGCAGAGT43330.12325279281498512No Hit
AAAAAGTACTCTGCGTTGATACCAC41510.11807577728479189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGA400.005283795314.2488055
AACTGCG400.005295776814.243947
TAGGACC1600.013.6558824
CGCAAAA3500.012.2155212
GTACAAA9600.011.7785731
GTCTTAG2000.011.4024511
GTATCGA1201.2694545E-711.08808318
TCACGAC600.005875602411.08398153
ACGCAAA4450.010.8899811
GTTATAC1752.0008883E-1110.8577783
TATCGAC1252.1790584E-710.66231819
GAACAAA18050.010.6338641
TAGACAG3000.010.4491245
GTAGGAC1201.5241403E-610.2922693
TCTAGCA2151.8189894E-1210.1638182
AAAAGTA10050.010.0209132
GTCTAGC1356.4308006E-79.8539711
CTTAGAC1856.493792E-109.7573273
GTATAGG2950.09.66309451
GTCCTAG2002.6375346E-109.5020421
Produced by FastQC (version 0.11.5)