##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294711_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3515539 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.03430597697821 33.0 33.0 33.0 27.0 33.0 2 30.953138338104058 33.0 33.0 33.0 27.0 33.0 3 31.071448787796125 33.0 33.0 33.0 27.0 33.0 4 34.39000221587643 37.0 37.0 37.0 27.0 37.0 5 34.84444376808222 37.0 37.0 37.0 33.0 37.0 6 34.886713815434845 37.0 37.0 37.0 33.0 37.0 7 34.92029785475285 37.0 37.0 37.0 33.0 37.0 8 34.84016931685298 37.0 37.0 37.0 33.0 37.0 9 34.501467058109725 37.0 37.0 37.0 27.0 37.0 10 34.63989561771324 37.0 37.0 37.0 27.0 37.0 11 34.90237229625386 37.0 37.0 37.0 33.0 37.0 12 34.85054581957418 37.0 37.0 37.0 33.0 37.0 13 34.69022104434057 37.0 37.0 37.0 27.0 37.0 14 35.9796088167419 40.0 37.0 40.0 27.0 40.0 15 36.18094465741953 40.0 37.0 40.0 27.0 40.0 16 36.15993934358288 40.0 37.0 40.0 27.0 40.0 17 36.17713755984502 40.0 37.0 40.0 27.0 40.0 18 36.15502004102358 40.0 37.0 40.0 27.0 40.0 19 36.12618662458303 40.0 37.0 40.0 27.0 40.0 20 36.06908954786165 40.0 37.0 40.0 27.0 40.0 21 35.98343810152583 40.0 37.0 40.0 27.0 40.0 22 35.93239443510654 40.0 37.0 40.0 27.0 40.0 23 35.59780505919576 37.0 37.0 40.0 27.0 40.0 24 35.95544495452902 40.0 37.0 40.0 27.0 40.0 25 34.8996225045434 37.0 33.0 40.0 22.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.45429660427812735 1101 2 0.5824131964791803 1101 3 0.4609231433536074 1101 4 0.5680195089771374 1101 5 0.428446295468774 1101 6 0.3399479836082264 1101 7 0.3556741374840655 1101 8 0.49422734962649173 1101 9 0.7617599880634742 1101 10 0.6306193319477487 1101 11 0.5101203277528938 1101 12 0.545585858170476 1101 13 0.5050851209446492 1101 14 1.250610395172103 1101 15 0.9235717796212057 1101 16 0.9835184030625257 1101 17 0.9573824458457096 1101 18 0.9269140690299551 1101 19 0.912728561839117 1101 20 0.9567475243369117 1101 21 0.9655581598067897 1101 22 0.940885919494697 1101 23 1.275927452240353 1101 24 1.0200379610132089 1101 25 1.073887994270855 1102 1 0.47347159267959427 1102 2 0.5960642620456014 1102 3 0.5449188596246195 1102 4 0.5584264902658518 1102 5 0.5234711681082374 1102 6 0.4341548760581162 1102 7 0.48012149542215354 1102 8 0.5273518447068 1102 9 0.7249891984780987 1102 10 0.7888022898670854 1102 11 0.4857034016399311 1102 12 0.5105484719269029 1102 13 0.5343683180517829 1102 14 1.2359010919786186 1102 15 1.0319745484118599 1102 16 0.8350175612799191 1102 17 0.9461808542224688 1102 18 0.9317347334264028 1102 19 0.9472750894432806 1102 20 1.0338406585339754 1102 21 1.03000474901512 1102 22 1.081455797441805 1102 23 1.362548368907106 1102 24 1.1865903173286299 1102 25 1.139527172501083 1103 1 0.2834615046103437 1103 2 0.46654851834166067 1103 3 0.30225438092747936 1103 4 0.17716885975109875 1103 5 0.2760872576805582 1103 6 0.23791950477254886 1103 7 0.20676158892077012 1103 8 0.42004793191500056 1103 9 0.5869041006018065 1103 10 0.6050583935545859 1103 11 0.40234002428645965 1103 12 0.42529321943835185 1103 13 0.37327848208575887 1103 14 1.103244777606335 1103 15 0.8331525521330292 1103 16 0.5910939571100329 1103 17 0.5801067819511019 1103 18 0.6380752674740648 1103 19 0.7153404689395231 1103 20 0.8305839678726556 1103 21 0.7844548855024698 1103 22 0.8071022653717677 1103 23 1.2046769804909445 1103 24 0.8696344226455182 1103 25 0.7252662003496013 1104 1 0.4049056806983806 1104 2 0.5652100790512833 1104 3 0.38065893805037376 1104 4 0.40163359181487834 1104 5 0.30063540203818206 1104 6 0.20067394741441547 1104 7 0.10420326826329074 1104 8 0.33243559476812834 1104 9 0.649256758408022 1104 10 0.6034008319489885 1104 11 0.41954038905123525 1104 12 0.2727216371633716 1104 13 0.45363938552524985 1104 14 0.9454401516917272 1104 15 0.7410826245142843 1104 16 0.7682761023805114 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2213 16 -0.20451080584865622 2213 17 -0.003115559716157179 2213 18 -0.11511888823834937 2213 19 -0.033792676267879074 2213 20 0.051717373926933874 2213 21 -0.5121801238379575 2213 22 -0.9975147244552076 2213 23 -0.2518887529235414 2213 24 -0.1364471631454407 2213 25 -0.7032487443324129 2214 1 -0.28287758289244636 2214 2 -0.399492746192216 2214 3 -0.4372456954206534 2214 4 -0.60509282640718 2214 5 -0.31400738790097904 2214 6 -0.23305446899762927 2214 7 -0.4248123767578136 2214 8 -0.20740006978684988 2214 9 -0.06996954780787235 2214 10 -1.8744875830293708 2214 11 -0.8552537436178298 2214 12 -0.5005003769762908 2214 13 -0.3756267052803892 2214 14 -0.8448979725186305 2214 15 -0.5737814896065601 2214 16 -0.7232763926688222 2214 17 -0.583653761595393 2214 18 -0.5629880597122607 2214 19 -1.4804889001638912 2214 20 -2.5362174826113417 2214 21 -1.967910405723238 2214 22 -2.0984858305841314 2214 23 -2.529779714457156 2214 24 -1.628850556348958 2214 25 -2.0238192534388943 2215 1 -0.38802903088698315 2215 2 -0.43198253464609593 2215 3 -0.8618899354239673 2215 4 -0.47733949267745857 2215 5 -0.29738423404221237 2215 6 -0.2652190375001169 2215 7 -0.22112851757714935 2215 8 -0.2863983382833908 2215 9 -0.41192436295501267 2215 10 -0.30481799208580185 2215 11 -0.6770239761726202 2215 12 -0.585673770560895 2215 13 -0.3565414291857678 2215 14 -0.34444460149221356 2215 15 -0.8005681273007212 2215 16 -0.5355440766162829 2215 17 -0.3743179670407102 2215 18 -0.5404940551105355 2215 19 -2.3197333989017466 2215 20 -2.437295888547098 2215 21 -3.488883038599461 2215 22 -2.444906789523536 2215 23 -3.4348913883219083 2215 24 -2.5074351829045582 2215 25 -1.797598597557645 2216 1 -1.4615520842260814 2216 2 -1.144371002080554 2216 3 -0.8739931022282086 2216 4 -0.9670362420190699 2216 5 -1.8164250212230044 2216 6 -1.733305328657437 2216 7 -1.13979654687369 2216 8 -4.898962702891705 2216 9 -4.111593859699113 2216 10 -2.0868152275194234 2216 11 -1.0244405478482577 2216 12 -1.2123623179188385 2216 13 -1.9602012040897918 2216 14 -3.294001091672108 2216 15 -2.005262321711321 2216 16 -3.0164623450281667 2216 17 -2.7094200987703303 2216 18 -2.7282929492839827 2216 19 -1.800669682852849 2216 20 -0.9487635981497107 2216 21 -0.8199038318072809 2216 22 -0.6923092763230585 2216 23 -1.0420985866762678 2216 24 -0.6009145403721661 2216 25 -0.37131818405188 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 5.0 7 50.0 8 291.0 9 671.0 10 1366.0 11 2685.0 12 4348.0 13 6945.0 14 10396.0 15 15394.0 16 20026.0 17 21896.0 18 22156.0 19 22432.0 20 22929.0 21 24384.0 22 25028.0 23 26013.0 24 27271.0 25 28753.0 26 30386.0 27 33197.0 28 37342.0 29 43579.0 30 52451.0 31 63746.0 32 86528.0 33 124442.0 34 182723.0 35 294493.0 36 523998.0 37 1759615.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.69326487734146 19.152834337561945 11.007201081698758 24.146699703397836 2 12.359813004346009 23.07294446461228 36.89852595258877 27.66871657845294 3 21.250579348642837 29.380579920522898 26.986280284392254 22.38256044644201 4 13.02581763821163 19.704123458490276 32.96595832098001 34.304100582318085 5 11.428566550929713 40.84218906446547 34.512554704276106 13.216689680328711 6 32.4262869539565 33.02991933077285 18.434783152913088 16.109010562357565 7 24.133540534336227 33.919407229904074 22.980110239808706 18.96694199595099 8 26.535220466420288 36.507098936809335 21.30542950900253 15.652251087767846 9 26.758004843783155 17.99103609966792 20.257498789623128 34.993460266925794 10 16.294075848662228 32.33416034366916 31.720644214691955 19.651119592976652 11 28.72985445226667 28.465736817088082 19.278197935473372 23.526210795171867 12 23.30626536245072 31.70431501212782 28.41516826621525 16.574251359206205 13 28.399521424938474 22.938776299724985 22.745457462459846 25.916244812876695 14 21.00233422481037 27.162030929189875 25.653233069189884 26.182401776809876 15 31.194177046747985 25.154751843780645 21.224349424983934 22.42672168448744 16 18.915535398181 29.456806228734933 31.24673405872194 20.38092431436212 17 25.445248323545123 30.141313184584412 27.2481164594957 17.165322032374768 18 25.73716108852191 26.37781948577791 28.40515826441368 19.479861161286504 19 22.800706693477252 31.518859050150944 28.9461580013466 16.734276255025208 20 23.555798920502628 28.08294635936311 27.161121027442558 21.200133692691704 21 19.266507676807418 30.426069857794513 30.036329287224568 20.2710931781735 22 21.46131785580689 29.57662838848455 22.62082280726027 26.341230948448292 23 17.73168405619689 31.05726445845729 27.241427412352497 23.969624072993323 24 20.609899018631776 35.58537903569905 24.207225145782964 19.59749679988622 25 22.04923094819785 27.000951727622287 21.983539502968945 28.966277821210923 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10947.0 1 10947.0 2 13149.0 3 15351.0 4 15351.0 5 15351.0 6 18677.5 7 22004.0 8 22004.0 9 22004.0 10 23039.0 11 24074.0 12 24074.0 13 24074.0 14 28666.5 15 33259.0 16 33259.0 17 33259.0 18 43724.0 19 54189.0 20 54189.0 21 54189.0 22 71248.5 23 88308.0 24 88308.0 25 88308.0 26 109889.0 27 131470.0 28 131470.0 29 131470.0 30 155682.0 31 179894.0 32 179894.0 33 179894.0 34 200921.5 35 221949.0 36 221949.0 37 221949.0 38 243357.0 39 264765.0 40 264765.0 41 264765.0 42 308508.0 43 352251.0 44 352251.0 45 352251.0 46 614415.5 47 876580.0 48 876580.0 49 876580.0 50 749420.5 51 622261.0 52 622261.0 53 622261.0 54 448509.0 55 274757.0 56 274757.0 57 274757.0 58 210729.5 59 146702.0 60 146702.0 61 146702.0 62 121433.0 63 96164.0 64 96164.0 65 96164.0 66 75382.0 67 54600.0 68 54600.0 69 54600.0 70 41109.5 71 27619.0 72 27619.0 73 27619.0 74 19680.0 75 11741.0 76 11741.0 77 11741.0 78 8068.5 79 4396.0 80 4396.0 81 4396.0 82 2971.0 83 1546.0 84 1546.0 85 1546.0 86 1040.0 87 534.0 88 534.0 89 534.0 90 335.0 91 136.0 92 136.0 93 136.0 94 80.5 95 25.0 96 25.0 97 25.0 98 21.0 99 17.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030350964674264742 2 0.016811077902990124 3 0.023211234465042203 4 0.027449560366134468 5 0.02730733466475553 6 0.009102444888251843 7 0.0031858557108881452 8 0.002360946642890322 9 0.0029867397289576364 10 0.0018489341179261558 11 0.0017067084165472207 12 0.0019342695387535168 13 0.0029298494484060623 14 7.111285068946753E-4 15 0.0020480500998566646 16 0.002560062624820831 17 0.002417836923441896 18 0.002645398045648192 19 3.413416833094441E-4 20 3.9823196386101816E-4 21 0.08379938325246854 22 0.0805850824013046 23 0.08217801025674867 24 0.0011093604707556935 25 0.23418883989055447 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3515539.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.69290179325944 #Duplication Level Percentage of deduplicated Percentage of total 1 88.19761577088723 45.59190690444103 2 8.39111048473027 8.67521700447103 3 1.698492500140563 2.634000180190615 4 0.5726821919787127 1.184144172348166 5 0.28007449664092415 0.7238931724827932 6 0.170243906282279 0.5280240917010427 7 0.11941183435896954 0.4320920958529823 8 0.0803392744653006 0.33223761800612 9 0.05862362840111092 0.2727382919132846 >10 0.3641367098279975 3.571637602245565 >50 0.0334037375283631 1.1964878766857792 >100 0.024840610037993646 2.5295636724000903 >500 0.0033982477650826167 1.2656540011696538 >1k 0.0034539567448262725 3.9688836050231315 >5k 5.013808177973622E-4 1.9122750464951095 >10k+ 0.0016712693926578736 25.181244664573622 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 142828 4.062762495310108 No Hit CCCATGTACTCTGCGTTGATACCAC 137111 3.9001416283534334 No Hit GAGTACATGGGAAGCAGTGGTATCA 65055 1.8504986006413242 No Hit CATGTACTCTGCGTTGATACCACTG 51739 1.4717231127289443 No Hit GCGTTGATACCACTGCTTCCCATGT 43351 1.2331252760956428 No Hit GCTTCCCATGTACTCTGCGTTGATA 37294 1.0608330614452008 No Hit GTATCAACGCAGAGTACATGGGAAG 36505 1.0383898457676048 No Hit ACGCAGAGTACATGGGAAGCAGTGG 35016 0.996035031896958 No Hit TATCAACGCAGAGTACATGGGAAGC 28567 0.8125923222584076 No Hit ACTCTGCGTTGATACCACTGCTTCC 28349 0.806391281678286 No Hit GGTATCAACGCAGAGTACATGGGAA 23706 0.6743204953778069 No Hit CAGTGGTATCAACGCAGAGTACATG 18931 0.5384949505609239 No Hit GTGGTATCAACGCAGAGTACATGGG 17727 0.5042470016688764 No Hit GCAGTGGTATCAACGCAGAGTACAT 15696 0.446474921768753 No Hit GTACATGGTAAGCAGTGGTATCAAC 14668 0.4172333175652439 No Hit ATACCACTGCTTCCCATGTACTCTG 14593 0.41509993204455986 No Hit GTACTCTGCGTTGATACCACTGCTT 13759 0.3913766850545535 No Hit CTGCTTCCCATGTACTCTGCGTTGA 13726 0.39043799542545254 No Hit CATGGGAAGCAGTGGTATCAACGCA 13472 0.38321292979540267 No Hit GGGAAGCAGTGGTATCAACGCAGAG 13041 0.37095307433653846 No Hit ACATGGGAAGCAGTGGTATCAACGC 12447 0.35405666101272093 No Hit TACCACTGCTTCCCATGTACTCTGC 11672 0.332011677298986 No Hit AAGCAGTGGTATCAACGCAGAGTAC 10978 0.31227074994758985 No Hit CTTCCCATGTACTCTGCGTTGATAC 10944 0.3113036151782131 No Hit GCAGAGTACATGGGAAGCAGTGGTA 10904 0.3101658095671816 No Hit GATACCACTGCTTCCCATGTACTCT 10767 0.30626882534939875 No Hit GTTGATACCACTGCTTCCCATGTAC 10718 0.3048750134758852 No Hit CCACTGCTTCCCATGTACTCTGCGT 10575 0.3008073584164477 No Hit CCATGTACTCTGCGTTGATACCACT 10150 0.2887181737992382 No Hit ACCATGTACTCTGCGTTGATACCAC 10133 0.2882346064145498 No Hit GTACATGGGTAAGCAGTGGTATCAA 9112 0.2591921181929713 No Hit ATGGGAAGCAGTGGTATCAACGCAG 8889 0.2528488519114708 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 8314 0.23649289625289321 No Hit TTCCCATGTACTCTGCGTTGATACC 7922 0.22534240126478472 No Hit TGATACCACTGCTTCCCATGTACTC 7303 0.20773485943407255 No Hit GGTAAGCAGTGGTATCAACGCAGAG 6889 0.19595857135989675 No Hit CCCCATGTACTCTGCGTTGATACCA 6816 0.1938820761197643 No Hit ATCAACGCAGAGTACATGGGAAGCA 5806 0.16515248444121938 No Hit GCTTACCATGTACTCTGCGTTGATA 5353 0.15226683589628787 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 4963 0.14117323118873096 No Hit GAGTACATGGTAAGCAGTGGTATCA 4826 0.13727624697094812 No Hit GGAAGCAGTGGTATCAACGCAGAGT 4333 0.12325279281498512 No Hit AAAAAGTACTCTGCGTTGATACCAC 4151 0.11807577728479189 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.844514027578701E-5 0.0 0.0 0.0 2.844514027578701E-5 6 2.844514027578701E-5 0.0 0.0 0.0 2.844514027578701E-5 7 2.844514027578701E-5 2.844514027578701E-5 0.0 0.0 2.844514027578701E-5 8 2.844514027578701E-5 2.844514027578701E-5 0.0 0.0 2.844514027578701E-5 9 2.844514027578701E-5 2.844514027578701E-5 0.0 5.689028055157402E-5 2.844514027578701E-5 10 2.844514027578701E-5 2.844514027578701E-5 0.0 1.4222570137893508E-4 2.844514027578701E-5 11 2.844514027578701E-5 2.844514027578701E-5 0.0 1.9911598193050908E-4 2.844514027578701E-5 12 2.844514027578701E-5 5.689028055157402E-5 0.0 3.1289654303365717E-4 2.844514027578701E-5 13 2.844514027578701E-5 5.689028055157402E-5 0.0 3.697868235852312E-4 2.844514027578701E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACGA 40 0.0052837953 14.248805 5 AACTGCG 40 0.0052957768 14.24394 7 TAGGACC 160 0.0 13.655882 4 CGCAAAA 350 0.0 12.215521 2 GTACAAA 960 0.0 11.778573 1 GTCTTAG 200 0.0 11.402451 1 GTATCGA 120 1.2694545E-7 11.088083 18 TCACGAC 60 0.0058756024 11.0839815 3 ACGCAAA 445 0.0 10.889981 1 GTTATAC 175 2.0008883E-11 10.857778 3 TATCGAC 125 2.1790584E-7 10.662318 19 GAACAAA 1805 0.0 10.633864 1 TAGACAG 300 0.0 10.449124 5 GTAGGAC 120 1.5241403E-6 10.292269 3 TCTAGCA 215 1.8189894E-12 10.163818 2 AAAAGTA 1005 0.0 10.020913 2 GTCTAGC 135 6.4308006E-7 9.853971 1 CTTAGAC 185 6.493792E-10 9.757327 3 GTATAGG 295 0.0 9.6630945 1 GTCCTAG 200 2.6375346E-10 9.502042 1 >>END_MODULE