FastQCFastQC Report
Wed 25 May 2016
SRR1294711_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294711_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3515539
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC1502294.273284978491207No Hit
GTACATGGGAAGCAGTGGTATCAAC1380193.9259698157238474No Hit
GAGTACATGGGAAGCAGTGGTATCA715362.0348515547686996No Hit
CATGTACTCTGCGTTGATACCACTG593501.6882190753679593No Hit
GCGTTGATACCACTGCTTCCCATGT466941.3282173800375987No Hit
ACGCAGAGTACATGGGAAGCAGTGG425651.2107673958388743No Hit
GCTTCCCATGTACTCTGCGTTGATA416191.1838582931379797No Hit
GTATCAACGCAGAGTACATGGGAAG377771.074572064198406No Hit
ACTCTGCGTTGATACCACTGCTTCC328080.9332281621680203No Hit
TATCAACGCAGAGTACATGGGAAGC322860.9183797989440594No Hit
GGTATCAACGCAGAGTACATGGGAA255790.7275982431143561No Hit
CAGTGGTATCAACGCAGAGTACATG229240.6520763956821415No Hit
GTGGTATCAACGCAGAGTACATGGG189580.5392629693483703No Hit
GCAGTGGTATCAACGCAGAGTACAT177560.5050719107368742No Hit
CATGGGAAGCAGTGGTATCAACGCA167840.47742323438880924No Hit
ATACCACTGCTTCCCATGTACTCTG164530.4680078929575237No Hit
ACATGGGAAGCAGTGGTATCAACGC159270.45304574917245977No Hit
GGGAAGCAGTGGTATCAACGCAGAG153820.4375431477221558No Hit
CTGCTTCCCATGTACTCTGCGTTGA145980.4152421577459388No Hit
GTACATGGTAAGCAGTGGTATCAAC138240.39322561917247967No Hit
GTACTCTGCGTTGATACCACTGCTT136970.38961308635745473No Hit
CTTCCCATGTACTCTGCGTTGATAC131460.3739398140654961No Hit
AAGCAGTGGTATCAACGCAGAGTAC130280.3705832875129532No Hit
TACCACTGCTTCCCATGTACTCTGC130110.3700997201282648No Hit
GCAGAGTACATGGGAAGCAGTGGTA125490.3569580653208512No Hit
GATACCACTGCTTCCCATGTACTCT123160.3503303476365929No Hit
CCATGTACTCTGCGTTGATACCACT121430.3454093383688817No Hit
CCACTGCTTCCCATGTACTCTGCGT120060.3415123541510989No Hit
ACCATGTACTCTGCGTTGATACCAC115630.3289111570089252No Hit
GTTGATACCACTGCTTCCCATGTAC110510.3143472451877223No Hit
ATGGGAAGCAGTGGTATCAACGCAG107170.3048465683356094No Hit
GTATCAACGCAGAGTACTTTTTTTT101170.2877794841701372No Hit
GTACATGGGTAAGCAGTGGTATCAA99020.281663779010843No Hit
TTCCCATGTACTCTGCGTTGATACC82540.23478618783634603No Hit
GGTAAGCAGTGGTATCAACGCAGAG80900.23012118483111693No Hit
TATCAACGCAGAGTACTTTTTTTTT78060.2220427649927934No Hit
TGATACCACTGCTTCCCATGTACTC77000.21902758012356002No Hit
CCCCATGTACTCTGCGTTGATACCA75020.21339544234895416No Hit
GGTATCAACGCAGAGTACTTTTTTT73790.20989669009503237No Hit
ATCAACGCAGAGTACATGGGAAGCA66790.18998509190198146No Hit
GCTTACCATGTACTCTGCGTTGATA59520.1693054749214843No Hit
GTACTTTTTTTTTTTTTTTTTTTTT59130.16819611445072863No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA58640.16680230257721504No Hit
ACGCAGAGTACTTTTTTTTTTTTTT56790.16153995162619444No Hit
GAGTACATGGTAAGCAGTGGTATCA54490.15499756936276343No Hit
GGAAGCAGTGGTATCAACGCAGAGT49640.14120167632900674No Hit
AAAAAGTACTCTGCGTTGATACCAC46000.13084764526862025No Hit
GAGTACTTTTTTTTTTTTTTTTTTT41700.11861623495003183No Hit
ACTCTGCGTTGATACCACTGCTTAC40700.11577172092245315No Hit
CTGCGTTGATACCACTGCTTCCCAT37710.10726662397999283No Hit
ATGGTAAGCAGTGGTATCAACGCAG36850.10482034191627514No Hit
TGGGAAGCAGTGGTATCAACGCAGA36300.10325585920110684No Hit
TCCATGTACTCTGCGTTGATACCAC36270.1031705237802795No Hit
GCGTTGATACCACTGCTTACCATGT35510.10100869311931969No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGC755.7394573E-815.2241883
GTTCCGG500.00147661313.3280031
TATTCCG1001.8950741E-611.418145
TCTAGAC2500.011.4181393
TACCGGG855.239954E-511.1945742
CCGGGGT855.241476E-511.1942554
CGCAAAA1752.0008883E-1110.874732
GTCTTAC1951.8189894E-1210.7405161
AGGACCG803.718191E-410.7045075
TAGACAG3950.010.5991175
GTACCGG1105.9010836E-610.3854571
GTATTAG2700.010.2251871
ATCTACG951.6185115E-410.0161982
GCTACAC2105.456968E-129.9682183
ACGCAAA2800.09.86000251
CTAGACT2800.09.8549424
GGCTAGA1901.0131771E-99.5200021
AAACCCG800.0044589719.5151175
CCGCGTT900.00109816199.5151174
CGGGTTC1201.7148259E-59.4938718
Produced by FastQC (version 0.11.5)